2,005 Works

Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway

Mingjun Yu, Yixue Xue, Jian Zheng, Xiaobai Liu, Hai Yu, Libo Liu, Zhen Li & Yunhui Liu
Abstract Background Glioma is one of the most frequent intracranial malignant tumors. LncRNAs have been identified as new modulators in the origination and progression of glioma. Methods Quantitative real-time PCR were conducted to evaluate the expression of linc00152 and miRNA-103a-3p in glioma tissues and cells. Western blot were used to determine the expression of FEZF1 and CDC25A in glioma tissues and cells. Stable knockdown of linc00152 or over-expression of miR-103a-3p in glioma stem cells (GSCs)...

A successful hybrid deep learning model aiming at promoter identification

Ying Wang, Qinke Peng, Xu Mou, Xinyuan Wang, Haozhou Li, Tian Han, Zhao Sun & Xiao Wang
Abstract Background The zone adjacent to a transcription start site (TSS), namely, the promoter, is primarily involved in the process of DNA transcription initiation and regulation. As a result, proper promoter identification is critical for further understanding the mechanism of the networks controlling genomic regulation. A number of methodologies for the identification of promoters have been proposed. Nonetheless, due to the great heterogeneity existing in promoters, the results of these procedures are still unsatisfactory. In...

Ultraviolet-to-Infrared Broadband Photodetector and Imaging Application based on Perovskite Single Crystal

Meng Xu, Xinyue Wang, Jiexin Weng, Jingling Shen, Yanbing Hou & Bo Zhang
The organic-inorganic hybrid perovskite CH3NH3PbBr3(MAPbBr3) has been well developed in the X-ray to visible light band due to its superior optoelectronic properties, but this material is rarely studied in the infrared band. In this paper, a UV-NIR broadband optical detector based on MAPbBr3 single crystal is studied, and the response range can reach the near-infrared region. In the visible light band, the optical response of the device is mainly caused by the photoelectric effect; in...

Ultraviolet-to-Infrared Broadband Photodetector and Imaging Application based on Perovskite Single Crystal

Meng Xu, Xinyue Wang, Jiexin Weng, Jingling Shen, Yanbing Hou & Bo Zhang
The organic-inorganic hybrid perovskite CH3NH3PbBr3(MAPbBr3) has been well developed in the X-ray to visible light band due to its superior optoelectronic properties, but this material is rarely studied in the infrared band. In this paper, a UV-NIR broadband optical detector based on MAPbBr3 single crystal is studied, and the response range can reach the near-infrared region. In the visible light band, the optical response of the device is mainly caused by the photoelectric effect; in...

Study on the prognosis, immune and drug resistance of m6A-related genes in lung cancer

Yang Yang, Zhouyao Qian, Mingyang Feng, Weiting Liao, Qiuji Wu, Feng Wen & Qiu Li
Abstract Background Few studies have demonstrated that the relationship between m6A-related genes and the prognosis, tumor microenvironment and drug resistance of LC. Methods The main results were analyzed with bioinformatics methods. Results Hence, we found 10 m6A-related genes expressed less in tumor samples in comparison with normal ones. Using consensus clustering, all LC patients were grouped into 2 subgroups according to the overall expression of 10 differential expressed m6A-related genes. In two clusters, the OS...

Genome-wide association study uncovers major genetic loci associated with flowering time in response to active accumulated temperature in wild soybean population

Guang Yang, Wei Li, Chao Fan, Miao Liu, Jianxin Liu, Wenwei Liang, Ling Wang, Shufeng Di, Chao Fang, Haiyang Li, Guohua Ding, Yingdong Bi & Yongcai Lai
Abstract Flowering time and active accumulated temperature (AAT) are two key factors that limit the expanded production especially for soybean across different regions. Wild soybean provides an important germplasm for functional genomics study in cultivar soybean. However, the studies on genetic basis underlying flowering time in response to AAT especially in wild soybean were rarely reported. In this study, we used 294 wild soybean accessions derived from major soybean production region characterized by different AAT...

Quantitative assessment of the effects of massive nucleic acid testing in controlling a COVID-19 outbreak

Wenlong Zhu, Yue Zhu, Zexuan Wen, Bo Zheng, Ao Xu, Ye Yao & Weibing Wang
Abstract Background From 20 July to 26 August 2021, local outbreaks of COVID-19 occurred in Nanjing City and Yangzhou City (Jiangsu Province, China). We analyzed the characteristics of these outbreaks in an effort to develop specific and effective intervention strategies. Methods Publicly available data on the characteristics of the COVID-19 outbreaks in Jiangsu Province were collected. Logistic regression was used to assess the association of age and sex with clinical severity. Analysis of onset dates,...

Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus

Congcong Lian, Min Zhang, Jiaqi Mao, Yuanyu Liu, Xiuwen Wang, Linghui Kong, Qingshou Yao & Jiayang Qin
Abstract ε-poly-l-lysine (ε-PL) is the main secondary metabolite of Streptomyces albulus, and it is widely used in the food industry. Polylysine synthetase (Pls) is the last enzyme in the ε-PL biosynthetic pathway. Our previous study revealed that Pls overexpressed in S. albulus CICC11022 result in the efficient production of ε-PL. In this study, a Pls gene knockout strain was initially constructed. Then, genomic, transcriptomic and metabolomic approaches were integrated to study the effects of the...

Diagnostic models for fever of unknown origin based on 18F-FDG PET/CT: a prospective study in China

Jia Chen, MingYou Xing, Dong Xu, NaNa Xie, WenCong Zhang, QiuRong Ruan & JianXin Song
Abstract Background This study aims to analyze the 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) characteristics of different causes of fever of unknown origin (FUO) and identify independent predictors to develop a suitable diagnostic model for distinguishing between these causes. A total of 524 patients with classical FUO who underwent standard diagnostic procedures and PET/CT were prospectively studied. The diagnostic performance of PET/CT imaging was analyzed, and relevant clinical parameters that could improve diagnostic efficacy...

Analysis of different adipose depot gene expression in cachectic patients with gastric cancer

Jun Han, Zuoyou Ding, Qiulin Zhuang, Lei Shen, Fan Yang, Szechun Sah & Guohao Wu
Abstract Purpose This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. Methods RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of...

Analysis of different adipose depot gene expression in cachectic patients with gastric cancer

Jun Han, Zuoyou Ding, Qiulin Zhuang, Lei Shen, Fan Yang, Szechun Sah & Guohao Wu
Abstract Purpose This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. Methods RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of...

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calves

Hongwei Chen, Yalu Liu, Kailang Huang, Bin Yang, Yuanyuan Zhang, Zhongtang Yu & Jiakun Wang
Abstract Background Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples...

Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus

Congcong Lian, Min Zhang, Jiaqi Mao, Yuanyu Liu, Xiuwen Wang, Linghui Kong, Qingshou Yao & Jiayang Qin
Abstract ε-poly-l-lysine (ε-PL) is the main secondary metabolite of Streptomyces albulus, and it is widely used in the food industry. Polylysine synthetase (Pls) is the last enzyme in the ε-PL biosynthetic pathway. Our previous study revealed that Pls overexpressed in S. albulus CICC11022 result in the efficient production of ε-PL. In this study, a Pls gene knockout strain was initially constructed. Then, genomic, transcriptomic and metabolomic approaches were integrated to study the effects of the...

Association of physical activity and air pollution exposure with the risk of type 2 diabetes: a large population-based prospective cohort study

Zhi-Hao Li, Wen-Fang Zhong, Xi-Ru Zhang, Vincent CH Chung, Wei-Qi Song, Qing Chen, Xiao-Meng Wang, Qing-Mei Huang, Dong Shen, Pei-Dong Zhang, Dan Liu, Yu-Jie Zhang, Pei-Liang Chen, Xin Cheng, Hai-Lian Yang, Miao-Chun Cai, Xiang Gao, Virginia Byers Kraus & Chen Mao
Abstract Background The interplay between physical activity (PA) and air pollution in relation to type 2 diabetes (T2D) remains largely unknown. Based on a large population-based cohort study, this study aimed to examine whether the benefits of PA with respect to the risk of T2D are moderated by exposure to air pollution. Methods UK Biobank participants (n = 359,153) without diabetes at baseline were included. Information on PA was obtained using the International Physical Activity...

Low barrier medication for opioid use disorder at a federally qualified health center: a retrospective cohort study

Jamie Carter, Zhen Li, Hillary Chen, Melissa Greiner, Christopher Bush, Debanjan Bhattacharya, Stephanie Poley, Nidhi Sachdeva, Jane Carolyn Crowder & Jacob Feigal
Abstract Background Medication for opioid use disorder (MOUD) reduces mortality, but few patients access MOUD. At a Federally Qualified Health Center (FQHC), we implemented a low barrier model of MOUD, including same-day MOUD initiation and a harm reduction philosophy. Objective To investigate whether low barrier MOUD improved retention in care compared to traditional treatment. Design and participants Retrospective cohort study of patients with at least one visit seeking MOUD at the FQHC during a historical...

SIRPα antibody combined with oncolytic virus OH2 protects against tumours by activating innate immunity and reprogramming the tumour immune microenvironment

Defeng Kong, Zhenrong Yang, Guoliang Li, Quanyou Wu, Zhaoru Gu, Duo Wan, Qi Zhang, Xiaoli Zhang, Shujun Cheng, Binlei Liu, Kaitai Zhang & Wen Zhang
Abstract Background The combination of oncolytic viruses (OVs) with immune checkpoint blockades is a research hotspot and has shown good efficacy. Here, we present the first attempt to combine oncolytic herpes simplex virus 2 (OH2) with an anti-SIRPα antibody as an antitumour treatment. Our results provide unique insight into the combination of innate immunity with OV. Methods We verified the polarization and activation of OH2 in RAW264.7 cells in vitro. Subsequently, we evaluated the antitumour...

Myofascial release for the treatment of pain and dysfunction in patients with chronic mechanical neck pain: Systematic review and meta-analysis of randomised controlled trials

Yaorui Guo, Xiuying Lv, Yu Zhou, Zhijun Li, Huiping She, Li Bai & Jing Bao
ObjectiveTo explore the effects of myofascial release (MFR) on pain and dysfunction in individuals with chronic mechanical neck pain (MNP).Data sourcesPubMed, Embase, Medline, Wiley Online Library, Web of Science, CNKI, VIP, WanFang Data, and the Cochrane Library were searched until 12 September 2022.Review methodsThis study was registered in PROSPERO (CRD42022302485). Methodological quality was assessed using Cochrane risk of bias assessment, and the quality of the evidence followed the GRADE recommendation. The outcomes pain, cervical mobility...

Application of exome sequencing for prenatal diagnosis of fetal structural anomalies: clinical experience and lessons learned from a cohort of 1618 fetuses

Fang Fu, Ru Li, Qiuxia Yu, Dan Wang, Qiong Deng, Lushan Li, Tingying Lei, Guilan Chen, Zhiqiang Nie, Xin Yang, Jin Han, Min Pan, Li Zhen, Yongling Zhang, Xiangyi Jing, Fucheng Li, Fatao Li, Lina Zhang, Cuixing Yi, Yingsi Li, Yan Lu, Hang Zhou, Ken Cheng, Jian Li, Lina Xiang … & Can Liao
Abstract Background Exome sequencing (ES) is becoming more widely available in prenatal diagnosis. However, data on its clinical utility and integration into clinical management remain limited in practice. Herein, we report our experience implementing prenatal ES (pES) in a large cohort of fetuses with anomalies detected by ultrasonography using a hospital-based in-house multidisciplinary team (MDT) facilitated by a three-step genotype-driven followed by phenotype-driven analysis framework. Methods We performed pES in 1618 fetal cases with positive...

Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Abstract Background Flavonoids are necessary for plant growth and resistance to adversity and stress. They are also an essential nutrient for human diet and health. Among the metabolites produced in Cannabis sativa (C. sativa), phytocannabinoids have undergone extensive research on their structures, biosynthesis, and biological activities. Besides the phytocannabinoids, C. sativa is also rich in terpenes, alkaloids, and flavonoids, although little research has been conducted in this area. Results In this study, we identified 11...

Establishment and evaluation of a CT-based radiomic model for AIDS-associated pulmonary cryptococcosis

Zi-xin Zhang, Xing-yu Mu, Jing Yu, Chun-shuang Guan, Bu-dong Chen & Ru-Ming Xie
Abstract Background Establish a CT-based diagnostic radiomic model for AIDS complicated with pulmonary cryptococcosis and evaluate the diagnostic efficacy of this model. Methods This retrospective study enrolled 98 AIDS patients with pulmonary cryptococcosis and 103 AIDS patients with other infections or neoplastic lesions, comprising a total of 699 lesions. Patients were randomly divided into a training group and test group at a ratio of 2.75:1. Features from all lesions, cavity lesions and solid nodule lesions...

H2O2/pH Dual-Responsive Biomimetic Nanoenzyme Drugs Delivery System for Enhanced Tumor Photodynamic Therapy

Xinyuan Li, Qing Ji, Chao Yan, Ziyu Zhu, Zhihui Yan, Ping Chen, Yisen Wang & Li Song
Abstract Phototherapy has been recognized as a photochemical process to treat tumor via induce cancer cells necrosis and death, with minimal invasiveness, higher selectivity, and few side effects. However, the therapy effects of phototherapy are often compromised by the hypoxia, high levels of hydrogen peroxide, and glutathione of tumor microenvironment (TME). Therefore, we constructed a catalase-like activity bionic metal–organic framework drugs delivery system (FA-EM@MnO2/ZIF-8/ICG) with tumor microenvironment controllable releasing. In this system, photosensitizer indocyanine green...

Using beam-offset optical coherence tomography to reconstruct backscattered photon profiles in scattering media

weiming xu & Hui Wang
To image the underlying structures of a scattering medium, raster scanning imaging technologies capture least scattered photons (LSPs) and reject multiple scattered photons (MSPs) in backscattered photons. However, MSPs can still squeeze into the images, resulting in limited imaging depth, degraded contrast, and significantly reduced lateral resolution. Great efforts have been made to understand how MSPs affect imaging performance through modeling, but the techniques for visualizing the backscattered photon profile (BSPP) in scattering media during...

Genomic characteristics of two breast malignant phyllodes tumors during pregnancy and lactation identified through whole-exome sequencing

Tinge Lei, Mengjia Shen, Xu Deng, Yongqiang Shi, Yan Peng, Hui Wang & Tongbing Chen
Abstract Background The genomic landscape of breast malignant phyllodes tumors (PTs) is not well defined, especially pregnancy-related malignant PTs. To clarify this topic, whole-exome next-generation sequencing (NGS) was performed on tumor samples and paired normal breast tissues from two pregnancy-related malignant PTs, followed by a functional analysis of the genetic alterations. Methods DNA from malignant PT samples and matched normal breast tissues of both patients were subjected to molecular profiling. NGS of the whole-exome was...

The multiple de novo copy number variant (MdnCNV) phenomenon presents with peri-zygotic DNA mutational signatures and multilocus pathogenic variation

Haowei Du, Angad Jolly, Christopher M. Grochowski, Bo Yuan, Moez Dawood, Shalini N. Jhangiani, He Li, Donna Muzny, Jawid M. Fatih, Zeynep Coban-Akdemir, Mary Esther Carlin, Angela E. Scheuerle, Karin Witzl, Jennifer E. Posey, Matthew Pendleton, Eoghan Harrington, Sissel Juul, P. J. Hastings, Weimin Bi, Richard A. Gibbs, Fritz J. Sedlazeck, James R. Lupski, Claudia M. B. Carvalho & Pengfei Liu
Abstract Background The multiple de novo copy number variant (MdnCNV) phenotype is described by having four or more constitutional de novo CNVs (dnCNVs) arising independently throughout the human genome within one generation. It is a rare peri-zygotic mutational event, previously reported to be seen once in every 12,000 individuals referred for genome-wide chromosomal microarray analysis due to congenital abnormalities. These rare families provide a unique opportunity to understand the genetic factors of peri-zygotic genome instability...

Efficacy and evaluation of therapeutic exercises on adults with Parkinson’s disease: a systematic review and network meta-analysis

Yong Yang, Guotuan Wang, Shikun Zhang, Huan Wang, Wensheng Zhou, Feifei Ren, Huimin Liang, Dongdong Wu, Xinying Ji, Makoto Hashimoto & Jianshe Wei
Abstract Background Exercises are an effective treatment in Parkinson’s disease (PD), but there is still controversy over which types should be used. We aimed to compare and rank the types of exercise that improve PD symptoms by quantifying information from randomised controlled trials. Methods We performed a systematic review and network meta-analysis and searched PubMed, MEDLINE, Embase, PsycINFO, Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science, and China National Knowledge Infrastructure (CNKI) from...

Registration Year

  • 2022
    2,005

Resource Types

  • Collection
    2,005

Affiliations

  • Chinese Academy of Medical Sciences & Peking Union Medical College
    2,005
  • Shanghai Jiao Tong University
    1,192
  • Zhejiang University
    1,137
  • Fudan University
    1,082
  • Sichuan University
    1,074
  • Huazhong University of Science and Technology
    963
  • Capital Medical University
    961
  • Sun Yat-sen University
    920
  • Chinese Academy of Sciences
    911
  • Nanjing Medical University
    853