427 Works

LncRNA SNHG16 promotes development of oesophageal squamous cell carcinoma by interacting with EIF4A3 and modulating RhoU mRNA stability

Lihua Ren, Xin Fang, Sachin Mulmi Shrestha, Qinghua Ji, Hui Ye, Yan Liang, Yang Liu, Yadong Feng, Jingwu Dong & Ruihua Shi
Abstract Background Numerous studies have revealed that long noncoding RNAs (lncRNAs) are closely related to the development of many diseases and carcinogenesis. However, their specific biological function and molecular mechanism in oesophageal squamous cell carcinoma (ESCC) remains unclear. Methods RNA-Seq was performed to determine the differential expressions of lncRNAs in ESCC, and the level of SNHG16 expression was detected in ESCC and intraepithelial neoplasia (IEN) samples. In vitro and in vivo experiments were performed to...

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72

Zhenguang Ying, Kaifang Wang, Junfeng Wu, Mingyu Wang, Jing Yang, Xia Wang, Guowei Zhou, Haibin Chen, Hongwu Xu, Stephen Cho Wing Sze, Feng Gao, Chunman Li & Ou Sha
Abstract Background The centrosome is one of the most important non-membranous organelles regulating microtubule organization and progression of cell mitosis. The coiled-coil alpha-helical rod protein 1 (CCHCR1, also known as HCR) gene is considered to be a psoriasis susceptibility gene, and the protein is suggested to be localized to the P-bodies and centrosomes in mammalian cells. However, the exact cellular function of HCR and its potential regulatory role in the centrosomes remain unexplored. Results We...

Simplified regimen of combined low-dose rituximab for autoimmune encephalitis with neuronal surface antibodies

Ying Du, Chao Zhao, Juntong Liu, Chuan Li, Qi Yan, Lin Li, Yunfeng Hao, Dan Yao, Huaxing Si, Yingjun Zhao & Wei Zhang
Abstract Background Autoimmune encephalitis (AE) with neuronal surface antibodies (NSAbs) presents pathogenesis mediated by B cell-secreting antibodies. Rituximab is a second-line choice for the treatment for AE with NSAbs, which can cause B cell depletion via targeting CD20. However, the optimal protocol and dosage of rituximab combined with first-line therapy for NSAbs-associated AE remains unclear so far. In this study, we explored the efficacy and safety of low-dose rituximab combined with first-line treatment for NSAbs-associated...

Simplified regimen of combined low-dose rituximab for autoimmune encephalitis with neuronal surface antibodies

Ying Du, Chao Zhao, Juntong Liu, Chuan Li, Qi Yan, Lin Li, Yunfeng Hao, Dan Yao, Huaxing Si, Yingjun Zhao & Wei Zhang
Abstract Background Autoimmune encephalitis (AE) with neuronal surface antibodies (NSAbs) presents pathogenesis mediated by B cell-secreting antibodies. Rituximab is a second-line choice for the treatment for AE with NSAbs, which can cause B cell depletion via targeting CD20. However, the optimal protocol and dosage of rituximab combined with first-line therapy for NSAbs-associated AE remains unclear so far. In this study, we explored the efficacy and safety of low-dose rituximab combined with first-line treatment for NSAbs-associated...

Development and validation of an extended Cox prognostic model for patients with ER/PR+ and HER2− breast cancer: a retrospective cohort study

Yiqun Xie, Xizhou Li, Ying Wu, Wenting Cui & Yang Liu
Abstract Background The purpose of this study was to explore a new estrogen receptor (ER) and/or progesterone receptor (PR)+ and human epidermal growth factor receptor 2 (HER2)− breast cancer prognostic model, called the extended Cox prognostic model, for determining the cutoff values for multiple continuous prognostic factors and their interaction via the new model concept and variable selection method. Methods A total of 335 patients with ER/PR+ and HER2− breast cancer were enrolled for the...

Endoplasmic reticulum-quality control pathway and endoplasmic reticulum-associated degradation mechanism regulate the N-glycoproteins and N-glycan structures in the diatom Phaeodactylum tricornutum

Jichen Chen, Hong Du, Zidong Liu, Tangcheng Li, Hua Du, Wanna Wang, Muhammad Aslam, Weizhou Chen, Ping Li, Haodong Luo, Hao Fang & Xiaojuan Liu
Abstract Tunicamycin inhibits the first step of protein N-glycosylation modification. However, the physiological, transcriptomic, and N-glycomic effects of tunicamycin on important marine diatom Phaeodactylum tricornutum are still unknown. In this study, comprehensive approaches were used to study the effects of tunicamycin stress. The results showed that cell growth and photosynthesis were significantly inhibited in P. tricornutum under the tunicamycin stress. The soluble protein content was significantly decreased, while the soluble sugar and neutral lipid were...

Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Abstract Background Flavonoids are necessary for plant growth and resistance to adversity and stress. They are also an essential nutrient for human diet and health. Among the metabolites produced in Cannabis sativa (C. sativa), phytocannabinoids have undergone extensive research on their structures, biosynthesis, and biological activities. Besides the phytocannabinoids, C. sativa is also rich in terpenes, alkaloids, and flavonoids, although little research has been conducted in this area. Results In this study, we identified 11...

Association between frequency of dairy product consumption and hypertension: a cross-sectional study in Zhejiang Province, China

Hao Wang, Lingli Chen, Yuan Cao, Kaixu Xie, Chunmei Wang, Pei Pei, Yu Guo, Fiona Bragg, Min Yu, Zhengming Chen & Liming Li
Abstract Background Hypertension, a well-known risk factor, contributes to millions of deaths from cardiovascular and renal diseases worldwide. However, evidence on the association between frequency of dairy product consumption and hypertension is inconsistent. Methods The data for the present study are from the Tongxiang baseline dataset of the China Kadoorie Biobank prospective study. A total of 53,916 participants aged 30–79 years were included in the final analysis. Multivariable logistic regression was utilized to evaluate the...

Local CpG density affects the trajectory and variance of age-associated DNA methylation changes

Jonathan Higham, Lyndsay Kerr, Qian Zhang, Rosie M. Walker, Sarah E. Harris, David M. Howard, Emma L. Hawkins, Anca-Larisa Sandu, J. Douglas Steele, Gordon D. Waiter, Alison D. Murray, Kathryn L. Evans, Andrew M. McIntosh, Peter M. Visscher, Ian J. Deary, Simon R. Cox & Duncan Sproul
Abstract Background DNA methylation is an epigenetic mark associated with the repression of gene promoters. Its pattern in the genome is disrupted with age and these changes can be used to statistically predict age with epigenetic clocks. Altered rates of aging inferred from these clocks are observed in human disease. However, the molecular mechanisms underpinning age-associated DNA methylation changes remain unknown. Local DNA sequence can program steady-state DNA methylation levels, but how it influences age-associated...

Risk factors and outcomes of postoperative stroke in surgical treatment for giant intracranial aneurysms

Hao Wang, Junlin Lu, Xin Chen & Qiang Hao
Abstract Background Giant intracranial aneurysms (GIAs) are challenges for surgical treatment. Risk factors of postoperative stroke remain unclear. This study aims to investigate the predictors of postoperative stroke in GIAs and the impact of stroke on outcomes. Methods We performed a retrospective medical record review of patients with GIAs who received microsurgery at our institution between 2011 and 2018. Multivariate logistic regression analyses were carried out to identify risk factors for postoperative stroke. The clinical...

The impact of circadian rhythms on the immune response to influenza vaccination in middle-aged and older adults (IMPROVE): a randomised controlled trial

Yihao Liu, Hui Zhang, Gang Yuan, Mi Yao, Bin Li, Jianying Chen, Yuling Fan, Ruohui Mo, Fenghua Lai, Xinwen Chen, Mengyuan Li, Binfeng Chen, Janet M. Lord, Sui Peng, KarKeung Cheng & Haipeng Xiao
Abstract Background Vaccination is important in influenza prevention but the immune response wanes with age. The circadian nature of the immune system suggests that adjusting the time of vaccination may provide an opportunity to improve immunogenicity. Our previous cluster trial in Birmingham suggested differences between morning and afternoon vaccination for some strains in the influenza vaccine in older adults. Whether this effect is also seen in a younger age group with less likelihood of compromised...

LncRNA PCED1B-AS1 knockdown inhibits osteosarcoma via methylation-mediated miR-10a downregulation

Bing Wang, Li Yao, Yuefu Dong, Jian Liu & Jian Wu
Abstract Background LncRNA PCED1B-AS1 (PCED1B-AS1) promotes glioma. This study aimed to investigate its role in osteosarcoma (OS). Methods The study included 60 OS patients. Accumulation of miR-10a and PCED1B-AS1 in tissues from OS patients and cell lines was determined by RT-qPCR. Cell transfections were performed for interaction analysis. Participation of PCED1B-AS1 siRNA silencing and miR-10a overexpression in proliferation, invasion, and migration of U2OS and MG-63 cells was analyzed by cell proliferation assay and Transwell assay....

PGE2 promotes macrophage recruitment and neovascularization in murine wet-type AMD models

Pengfei Zhan, Yuqing Cui, Yujuan Cao, Xun Bao, Meili Wu, Qian Yang, Jiahui Yang, Haohan Zheng, Jian Zou, Tianhua Xie, Jiping Cai, Yong Yao & Xiaolu Wang
Abstract Age-related macular degeneration (AMD), a progressive chronic disease of the central retina, is a leading cause of blindness worldwide. Activated macrophages recruited to the injured eyes greatly contribute to the pathogenesis of choroidal neovascularization (CNV) in exudative AMD (wet AMD). This study describes the effects of cyclooxygenase-2 (COX2)/prostaglandin E2 (PGE2) signalling on the macrophage activation and CNV formation of wet AMD. In a mouse model of laser-induced wet AMD, the mice received an intravitreal...

Saccharomyces cerevisiae employs complex regulation strategies to tolerate low pH stress during ethanol production

Yajing Wu, Bo Li, Bu Miao, Caiyun Xie & Yue-Qin Tang
Abstract Background Industrial bioethanol production may involve a low pH environment caused by inorganic acids, improving the tolerance of Saccharomyces cerevisiae to a low pH environment is of industrial importance to increase ethanol yield, control bacterial contamination, and reduce production cost. In our previous study, acid tolerance of a diploid industrial Saccharomyces cerevisiae strain KF-7 was chronically acclimatized by continuous ethanol fermentation under gradually increasing low-pH stress conditions. Two haploid strains B3 and C3 having...

Impact of antiretroviral therapy (ART) duration on ART adherence among men who have sex with men (MSM) living with HIV in Jinan of China

Kedi Jiao, Meizhen Liao, Guangmei Liu, Yanmei Bi, Xiuhong Zhao, Qian Chen, Jing Ma, Yu Yan, Chunxiao Cheng, Yijun Li, Wenwen Jia, Lina Wang, Yanwen Cao, Zhonghui Zhao, Xuan Yang, Jing Meng, Jianzhuo Li, Xinrui Li, Chunmei Wang, Dianmin Kang & Wei Ma
Abstract Background Consistent and complete adherence is considered an essential requirement for patients on antiretroviral therapy (ART). This study aimed to evaluate the impact of ART duration on ART adherence, identify the trend of complete adherence, and compare the factors associated with ART adherence between short-term and long-term ART group among men who have sex with men (MSM) living with HIV in Jinan of China. Methods MSM living with HIV aged 18 or above and...

MiRNA/mRNA network topology in hepatitis virus B-related liver cirrhosis reveals miR-20a-5p/340-5p as hubs initiating fibrosis

Heng Yao, Peng Li, Jiaojiao Xin, Xi Liang, Jing Jiang, Dongyan Shi, Jiang Li, Hozeifa Mohamed Hassan, Xin Chen & Jun Li
Abstract Background The pathophysiology of hepatitis B-related liver cirrhosis (HBV-LC) remains unclear. This study aimed to explore the disease mechanisms using topological analysis of the miRNA/mRNA network. Methods Paired miRNA/mRNA sequencing was performed with thirty-three peripheral blood mononuclear cell samples (LC, n = 9; chronic hepatitis B, n = 12; normal controls, n = 12) collected from a prospective cohort to identify the miRNA/mRNA network. Topological features and functional implications of the network were analyzed...

Multidimensional self-rating biological rhythm disorder and its association with depression and anxiety symptoms among adolescents aged 11–23 years: a school-based cross-sectional study from China

Xin Zeng, Yiyang Chen, Qian Zhang, Yexin Jin, Yalin Song, Kunyu Xue, Hao Lou, Ran Li, Xiaomin Lou & Xian Wang
Abstract Background Depression and anxiety are topical concerns worldwide, especially among adolescents. Besides, biological rhythm disorder as a candidate mechanism for mood disorders is highly prevalent, but relevant research among adolescents in China is presently limited. We conducted the present study to investigate the distribution of multi-dimensional self-rating biological rhythm disorder and the association of self-rating biological rhythm disorders with depression and anxiety symptoms among Chinese adolescents in different academic stages. Methods In the cross-sectional...

Prevalence Difference of Helicobacter pylori Infection Between Tibetan and Han Ethnics in China: A Meta-analysis on Epidemiologic Studies (SIGES)

Dan Bai, Kai Liu, Rui Wang, Wei-Han Zhang, Xin-Zu Chen & Jian-Kun Hu
China is a multi-ethnic country, and the prevalence of Helicobacter pylori (H pylori) infection may be diverse among ethnics. This meta-analysis was conducted to compare the prevalence of H pylori infection between Tibetans and Han ethnics. Ten studies that reported the prevalence of H pylori infection between Tibetans and Hans in China were eligible. The pooled prevalence of H pylori infection was 62.2% versus 55.3% among Tibetans and Hans, respectively. Tibetans had a higher risk...

Identification of circular RNA BTBD7_hsa_circ_0000563 as a novel biomarker for coronary artery disease and the functional discovery of BTBD7_hsa_circ_0000563 based on peripheral blood mononuclear cells: a case control study

Hanxiao Zhou, Xiongkang Gan, Shu He, Yanjun Wang, Sheng Zhang, Jiaxin Chen, Yaqing Zhou, Can Hou, Lei Hua, Qian Zhang & Enzhi Jia
Abstract Background BTBD7_hsa_circ_0000563 is a novel circRNA and contains conserved binding sites with RNA-binding proteins. However, BTBD7_hsa_circ_0000563 has not been fully studied in coronary artery disease (CAD). We aimed to clarify the diagnostic value and the possible functional role of BTBD7_hsa_circ_0000563 in CAD. Methods A total of 276 human peripheral blood mononuclear cell (PBMC) samples were employed. The circularization of BTBD7_hsa_circ_0000563 was verified via Sanger sequencing. The expression level of BTBD7_hsa_circ_0000563 in CAD samples and...

Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1–7) as a sensitizer for chemotherapy and immunotherapy in breast cancer

Jie Mei, Yun Cai, Rui Xu, Xinqian Yu, Xu Han, Miaomiao Weng, Lingyan Chen, Tao Ma, Tianshu Gao, Fei Gao, Tiansong Xia, Yichao Zhu & Yan Zhang
Abstract Background Angiotensin-converting enzyme 2 (ACE2) is known as a tumor suppressor and lowly expressed in most cancers. The expression pattern and role of ACE2 in breast cancer (BC) have not been deeply elucidated. Methods A systematic pan-cancer analysis was conducted to assess the expression pattern and immunological role of ACE2 based on RNA-sequencing (RNA-seq) data downloaded from The Cancer Genome Atlas (TCGA). The correlation of ACE2 expression and immunological characteristics in the BC tumor...

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calves

Hongwei Chen, Yalu Liu, Kailang Huang, Bin Yang, Yuanyuan Zhang, Zhongtang Yu & Jiakun Wang
Abstract Background Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples...

Multiomics analyses reveals Anaplasma phagocytophilum Ats-1 induces anti-apoptosis and energy metabolism by upregulating the respiratory chain-mPTP axis in eukaryotic mitochondria

Ruirui Li, Zhongchen Ma, Wei Zheng, Zhen Wang, Jihai Yi, Yangyang Xiao, Yong Wang & Chuangfu Chen
Abstract Background Anaplasma translocated substrate 1 (Ats-1) is an effector of type 4 secretory systems (T4SS) and the main virulence factor of Anaplasma phagocytophilum. Ats-1 is involved in the regulation of host cell biological processes, but the specific molecular mechanism of its action is unclear. Results In this study, we identified Ats-1 as involved in mitochondrial respiratory regulation of HEK293T cells by multi-omics analysis. After intracellular expression of Ats-1, adenosine triphosphate levels and the proliferation...

Combination of polythyleneimine regulating autophagy prodrug and Mdr1 siRNA for tumor multidrug resistance

Changduo Wang, Zhipeng Li, Ping Xu, Lisa Xu, Shangcong Han & Yong Sun
Abstract Multidrug resistance (MDR) has been restricting the efficacy of chemotherapy, which mainly include pump resistance and non-pump resistance. In order to fight overall MDR, a novel targeted gene/drug co-deliver nano system is developed, which can suppress the drug efflux pumps and modulate autophagy to overcoming both pump and non-pump resistance. Here, small interfere RNA (siRNA) is incorporated into polymer-drug conjugates (PEI-PTX, PP) which are composed of polyethyleneimine (PEI) and paclitaxel (PTX) via covalent bonds,...

Optical trapping of multiple particles based on rotationally-symmetric power-exponent-phase vortex beam

Ziheng Wu, Jiang Zhao, Jiantai Dou, Jun Liu, Qingli Jing, Bo Li & Youyou Hu
In this paper, the optical trapping of multiple particles based on rotationally-symmetric power-exponent-phase vortex beam (RSPEPVB) was introduced and demonstrated. Based on the theories of tight focusing and optical force, the optical force model of RSPEPVB was established to analyze the optical trap force of tightly focused RSPEPVB. Then, an experimental setup of optical tweezer, by utilizing the RSPEPVB, was built to demonstrate that the optical tweezer of RSPEPVBs can achieve the optical trapping of...

Immunogenicity and antigenicity of a conserved fragment of the rhoptry-associated membrane antigen of Plasmodium vivax

Jieyun Ge, Qiubo Wang, Gangcheng Chen, Kokouvi Kassegne, Hangye Zhang, Jiali Yu, Jianxia Tang, Bo Wang, Feng Lu, Jun Cao, Eun-Taek Han & Yang Cheng
Abstract Background Plasmodium vivax rhoptry-associated membrane antigen (RAMA) is a glycophosphatidylinositol-anchored membrane protein currently under consideration as a malaria vaccine candidate. Immunoglobulin G (IgG) antibodies induced by P. vivax RAMA (PvRAMA) have been proved to persist over 12 months in the sera of people infected with P. vivax. It has also been shown that through stimulation of peripheral blood mononuclear cells with PvRAMA in vitro, the antigen can induce CD4+ T cells to produce interleukin-10....

Registration Year

  • 2023
  • 2022
  • 2021

Resource Types

  • Collection


  • Guizhou University
  • Zhejiang University
  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Sun Yat-sen University
  • Sichuan University
  • Capital Medical University
  • Fudan University
  • Chinese Academy of Sciences
  • University of Chinese Academy of Sciences