24 Works

Psychological stress and strain in laboratory animal professionals – a systematic review

Sonja Rumpel, Regina Kempen, Roswitha Merle & Christa Thoene-Reineke
Laboratory animal professionals (LAP) are faced with various situations and tasks influencing their mental well-being. A systematic review has been conducted to investigate whether there are specific stressors for LAP and which moderators are relevant for the development of psychological strain. A comprehensive search following PRISMA Guidelines was carried out in June 2021. Results include 12 studies and have been summarized qualitatively in narrative synthesis and tabular presentation. Available literature indicates that LAP are facing...

Domain-Specific Greed

Martin Weiß, Julian Schulze, Stefan Krumm, Anja S. Göritz, Johannes Hewig & Patrick Mussel
Greed, the insatiable and excessive desire and striving for more even at the expense of others, may be directed toward various goods. In this article, we propose that greed may be conceptualized as a domain-specific construct. Based on a literature review and an expert survey, we identified 10 domains of greed which we operationalized with the DOmain-SPEcific Greed (DOSPEG) questionnaire. In Study 1 (N = 725), we found support for the proposed structure and convergent...

Do narcissistic admiration and rivalry matter for the mental health of romantic partners? Insights from actor-partner interdependence models

Leopold Maria Lautenbacher, Michael Eid & David Richter
Personality traits of romantic partners may be part of the puzzle of how romantic relationships are related to mental health. We investigated the role of narcissistic admiration and rivalry in this context. Positive associations of admiration and negative associations of rivalry with the mental health of individuals and their partners were hypothesized. Furthermore, we expected admiration to be particularly relevant for the narcissist’s mental health and rivalry to matter more for the partner. Actor-partner interdependence...

Individual immune cell and cytokine profiles determine platelet-rich plasma composition

Marcel Niemann, Melanie Ort, Luis Lauterbach, Mathias Streitz, Andreas Wilhelm, Gerald Grütz, Florian N. Fleckenstein, Frank Graef, Antje Blankenstein, Simon Reinke, Ulrich Stöckle, Carsten Perka, Georg N. Duda, Sven Geißler, Tobias Winkler & Tazio Maleitzke
Abstract Objective Platelet-rich plasma (PRP) therapy is increasingly popular to treat musculoskeletal diseases, including tendinopathies and osteoarthritis (OA). To date, it remains unclear to which extent PRP compositions are determined by the immune cell and cytokine profile of individuals or by the preparation method. To investigate this, we compared leukocyte and cytokine distributions of different PRP products to donor blood samples and assessed the effect of pro-inflammatory cytokines on chondrocytes. Design For each of three...

FKBP12 is a major regulator of ALK2 activity in multiple myeloma cells

Ingrid Quist-Løkken, Clara Andersson-Rusch, Martin Haugrud Kastnes, Jürgen Markus Kolos, Jerome Jatzlau, Hanne Hella, Oddrun Elise Olsen, Anders Sundan, Petra Knaus, Felix Hausch & Toril Holien
Abstract Background The immunophilin FKBP12 binds to TGF-β family type I receptors, including the BMP type I receptor ALK2. FKBP12 keeps the type I receptor in an inactive state and controls signaling activity. Removal of FKBP12 with drugs such as the FKBP-ligand FK506 enhances BMP activity in various cell types. In multiple myeloma cells, activation of SMAD1/5/8 leads to apoptosis. We hypothesized that removing FKBP12 from ALK2 in myeloma cells would potentiate BMP-induced ALK2-SMAD1/5/8 activity...

LINC00478-derived novel cytoplasmic lncRNA LacRNA stabilizes PHB2 and suppresses breast cancer metastasis via repressing MYC targets

Rong Guo, Yonghui Su, Qi Zhang, Bingqiu Xiu, Sheng Huang, Weiru Chi, Liyi Zhang, Lun Li, Jianjing Hou, Jia Wang, Jiajian Chen, Yayun Chi, Jingyan Xue & Jiong Wu
Abstract Background Metastasis is the predominant cause of mortality in patients with breast cancer. Long noncoding RNAs (lncRNAs) have been shown to drive important phenotypes in tumors, including invasion and metastasis. However, the lncRNAs involved in metastasis and their molecular and cellular mechanisms are still largely unknown. Methods The transcriptional and posttranscriptional processing of LINC00478-associated cytoplasmic RNA (LacRNA) was determined by RT-qPCR, semiquantitative PCR and 5′/3′ RACE. Paired-guide CRISPR/cas9 and CRISPR/dead-Cas9 systems was used to...

UmetaFlow: an untargeted metabolomics workflow for high-throughput data processing and analysis

Eftychia E. Kontou, Axel Walter, Oliver Alka, Julianus Pfeuffer, Timo Sachsenberg, Omkar S. Mohite, Matin Nuhamunada, Oliver Kohlbacher & Tilmann Weber
Abstract Metabolomics experiments generate highly complex datasets, which are time and work-intensive, sometimes even error-prone if inspected manually. Therefore, new methods for automated, fast, reproducible, and accurate data processing and dereplication are required. Here, we present UmetaFlow, a computational workflow for untargeted metabolomics that combines algorithms for data pre-processing, spectral matching, molecular formula and structural predictions, and an integration to the GNPS workflows Feature-Based Molecular Networking and Ion Identity Molecular Networking for downstream analysis. UmetaFlow...

Do narcissistic admiration and rivalry matter for the mental health of romantic partners? Insights from actor-partner interdependence models

Leopold Maria Lautenbacher, Michael Eid & David Richter
Personality traits of romantic partners may be part of the puzzle of how romantic relationships are related to mental health. We investigated the role of narcissistic admiration and rivalry in this context. Positive associations of admiration and negative associations of rivalry with the mental health of individuals and their partners were hypothesized. Furthermore, we expected admiration to be particularly relevant for the narcissist’s mental health and rivalry to matter more for the partner. Actor-partner interdependence...

Psychological stress and strain in laboratory animal professionals – a systematic review

Sonja Rumpel, Regina Kempen, Roswitha Merle & Christa Thoene-Reineke
Laboratory animal professionals (LAP) are faced with various situations and tasks influencing their mental well-being. A systematic review has been conducted to investigate whether there are specific stressors for LAP and which moderators are relevant for the development of psychological strain. A comprehensive search following PRISMA Guidelines was carried out in June 2021. Results include 12 studies and have been summarized qualitatively in narrative synthesis and tabular presentation. Available literature indicates that LAP are facing...

Humoral immune responses remain quantitatively impaired but improve qualitatively in anti-CD20-treated patients with multiple sclerosis after three or four COVID-19 vaccinations

Carolin Otto, Tatjana Schwarz, Lara M Jeworowski, Marie L Schmidt, Felix Walper, Florence Pache, Patrick Schindler, Moritz Niederschweiberer, Andi Krumbholz, Ruben Rose, Christian Drosten, Klemens Ruprecht & Victor M Corman
Objective:To analyze anti-SARS-CoV-2-S1-IgG levels, avidity, Omicron BA.2 variant neutralizing capacity, and SARS-CoV-2-specific T cells in anti-CD20-treated patients with multiple sclerosis (aCD20pwMS) after two, three, or four COVID-19 vaccinations.Results:Frequencies of aCD20pwMS with detectable SARS-CoV-2-S1-IgG increased moderately between two (31/61 (51%)), three (31/57 (54%)), and four (17/26 (65%)) vaccinations. However, among patients with detectable SARS-CoV-2-S1-IgG, frequencies of high avidity (6/31 (19%) vs 11/17 (65%)) and Omicron neutralizing antibodies (0/10 (0%) vs 6/10 (60%)) increased strongly between two...

Daily Emotion Regulation in Major Depression and Borderline Personality Disorder

Ulrike Zetsche, Paul-Christian Bürkner, Julian Bohländer, Babette Renneberg, Stefan Roepke & Lars Schulze
Emotional disturbances are an inherent aspect of most mental disorders and possibly driven by impaired emotion regulation. In the present study, we examined how exactly affected individuals differ from healthy individuals in regulating their emotions and whether individuals suffering from different mental disorders face similar or distinct difficulty in emotion regulation. We overcome earlier methodological constraints by using a 7-day experience sampling assessing the employment and effectiveness of six regulation strategies real time in 55...

Humoral immune responses remain quantitatively impaired but improve qualitatively in anti-CD20-treated patients with multiple sclerosis after three or four COVID-19 vaccinations

Carolin Otto, Tatjana Schwarz, Lara M Jeworowski, Marie L Schmidt, Felix Walper, Florence Pache, Patrick Schindler, Moritz Niederschweiberer, Andi Krumbholz, Ruben Rose, Christian Drosten, Klemens Ruprecht & Victor M Corman
Objective:To analyze anti-SARS-CoV-2-S1-IgG levels, avidity, Omicron BA.2 variant neutralizing capacity, and SARS-CoV-2-specific T cells in anti-CD20-treated patients with multiple sclerosis (aCD20pwMS) after two, three, or four COVID-19 vaccinations.Results:Frequencies of aCD20pwMS with detectable SARS-CoV-2-S1-IgG increased moderately between two (31/61 (51%)), three (31/57 (54%)), and four (17/26 (65%)) vaccinations. However, among patients with detectable SARS-CoV-2-S1-IgG, frequencies of high avidity (6/31 (19%) vs 11/17 (65%)) and Omicron neutralizing antibodies (0/10 (0%) vs 6/10 (60%)) increased strongly between two...

Genome-wide association reveals host-specific genomic traits in Escherichia coli

Sumeet K. Tiwari, Boas C. L. van der Putten, Thilo M. Fuchs, Trung N. Vinh, Martin Bootsma, Rik Oldenkamp, Roberto La Ragione, Sebastien Matamoros, Ngo T. Hoa, Christian Berens, Joy Leng, Julio Álvarez, Marta Ferrandis-Vila, Jenny M. Ritchie, Angelika Fruth, Stefan Schwarz, Lucas Domínguez, María Ugarte-Ruiz, Astrid Bethe, Charlotte Huber, Vanessa Johanns, Ivonne Stamm, Lothar H. Wieler, Christa Ewers, Amanda Fivian-Hughes … & Constance Schultsz
Abstract Background Escherichia coli is an opportunistic pathogen which colonizes various host species. However, to what extent genetic lineages of E. coli are adapted or restricted to specific hosts and the genomic determinants of such adaptation or restriction is poorly understood. Results We randomly sampled E. coli isolates from four countries (Germany, UK, Spain, and Vietnam), obtained from five host species (human, pig, cattle, chicken, and wild boar) over 16 years, from both healthy and...

Identification of ferroptosis related biomarkers and immune infiltration in Parkinson’s disease by integrated bioinformatic analysis

Na Xing, Ziye Dong, Qiaoli Wu, Yufeng Zhang, Pengcheng Kan, Yuan Han, Xiuli Cheng, Yaru Wang & Biao Zhang
Abstract Background Increasing evidence has indicated that ferroptosis engages in the progression of Parkinson’s disease (PD). This study aimed to explore the role of ferroptosis-related genes (FRGs), immune infiltration and immune checkpoint genes (ICGs) in the pathogenesis and development of PD. Methods The microarray data of PD patients and healthy controls (HC) from the Gene Expression Omnibus (GEO) database was downloaded. Weighted gene co-expression network analysis (WGCNA) was processed to identify the significant modules related...

Identification of ferroptosis related biomarkers and immune infiltration in Parkinson’s disease by integrated bioinformatic analysis

Na Xing, Ziye Dong, Qiaoli Wu, Yufeng Zhang, Pengcheng Kan, Yuan Han, Xiuli Cheng, Yaru Wang & Biao Zhang
Abstract Background Increasing evidence has indicated that ferroptosis engages in the progression of Parkinson’s disease (PD). This study aimed to explore the role of ferroptosis-related genes (FRGs), immune infiltration and immune checkpoint genes (ICGs) in the pathogenesis and development of PD. Methods The microarray data of PD patients and healthy controls (HC) from the Gene Expression Omnibus (GEO) database was downloaded. Weighted gene co-expression network analysis (WGCNA) was processed to identify the significant modules related...

Domain-Specific Greed

Martin Weiß, Julian Schulze, Stefan Krumm, Anja S. Göritz, Johannes Hewig & Patrick Mussel
Greed, the insatiable and excessive desire and striving for more even at the expense of others, may be directed toward various goods. In this article, we propose that greed may be conceptualized as a domain-specific construct. Based on a literature review and an expert survey, we identified 10 domains of greed which we operationalized with the DOmain-SPEcific Greed (DOSPEG) questionnaire. In Study 1 (N = 725), we found support for the proposed structure and convergent...

Individual immune cell and cytokine profiles determine platelet-rich plasma composition

Marcel Niemann, Melanie Ort, Luis Lauterbach, Mathias Streitz, Andreas Wilhelm, Gerald Grütz, Florian N. Fleckenstein, Frank Graef, Antje Blankenstein, Simon Reinke, Ulrich Stöckle, Carsten Perka, Georg N. Duda, Sven Geißler, Tobias Winkler & Tazio Maleitzke
Abstract Objective Platelet-rich plasma (PRP) therapy is increasingly popular to treat musculoskeletal diseases, including tendinopathies and osteoarthritis (OA). To date, it remains unclear to which extent PRP compositions are determined by the immune cell and cytokine profile of individuals or by the preparation method. To investigate this, we compared leukocyte and cytokine distributions of different PRP products to donor blood samples and assessed the effect of pro-inflammatory cytokines on chondrocytes. Design For each of three...

Genome-wide association reveals host-specific genomic traits in Escherichia coli

Sumeet K. Tiwari, Boas C. L. van der Putten, Thilo M. Fuchs, Trung N. Vinh, Martin Bootsma, Rik Oldenkamp, Roberto La Ragione, Sebastien Matamoros, Ngo T. Hoa, Christian Berens, Joy Leng, Julio Álvarez, Marta Ferrandis-Vila, Jenny M. Ritchie, Angelika Fruth, Stefan Schwarz, Lucas Domínguez, María Ugarte-Ruiz, Astrid Bethe, Charlotte Huber, Vanessa Johanns, Ivonne Stamm, Lothar H. Wieler, Christa Ewers, Amanda Fivian-Hughes … & Constance Schultsz
Abstract Background Escherichia coli is an opportunistic pathogen which colonizes various host species. However, to what extent genetic lineages of E. coli are adapted or restricted to specific hosts and the genomic determinants of such adaptation or restriction is poorly understood. Results We randomly sampled E. coli isolates from four countries (Germany, UK, Spain, and Vietnam), obtained from five host species (human, pig, cattle, chicken, and wild boar) over 16 years, from both healthy and...

UmetaFlow: an untargeted metabolomics workflow for high-throughput data processing and analysis

Eftychia E. Kontou, Axel Walter, Oliver Alka, Julianus Pfeuffer, Timo Sachsenberg, Omkar S. Mohite, Matin Nuhamunada, Oliver Kohlbacher & Tilmann Weber
Abstract Metabolomics experiments generate highly complex datasets, which are time and work-intensive, sometimes even error-prone if inspected manually. Therefore, new methods for automated, fast, reproducible, and accurate data processing and dereplication are required. Here, we present UmetaFlow, a computational workflow for untargeted metabolomics that combines algorithms for data pre-processing, spectral matching, molecular formula and structural predictions, and an integration to the GNPS workflows Feature-Based Molecular Networking and Ion Identity Molecular Networking for downstream analysis. UmetaFlow...

Daily Emotion Regulation in Major Depression and Borderline Personality Disorder

Ulrike Zetsche, Paul-Christian Bürkner, Julian Bohländer, Babette Renneberg, Stefan Roepke & Lars Schulze
Emotional disturbances are an inherent aspect of most mental disorders and possibly driven by impaired emotion regulation. In the present study, we examined how exactly affected individuals differ from healthy individuals in regulating their emotions and whether individuals suffering from different mental disorders face similar or distinct difficulty in emotion regulation. We overcome earlier methodological constraints by using a 7-day experience sampling assessing the employment and effectiveness of six regulation strategies real time in 55...

Guts within guts: the microbiome of the intestinal helminth parasite Ascaris suum is derived but distinct from its host

Ankur Midha, Víctor Hugo Jarquín-Díaz, Friederike Ebner, Ulrike Löber, Rima Hayani, Arkadi Kundik, Alessio Cardilli, Emanuel Heitlinger, Sofia Kirke Forslund & Susanne Hartmann
Abstract Background Intestinal helminths are extremely prevalent among humans and animals. In particular, intestinal roundworms affect more than 1 billion people around the globe and are a major issue in animal husbandry. These pathogens live in intimate contact with the host gut microbiota and harbor bacteria within their own intestines. Knowledge of the bacterial host microbiome at the site of infection is limited, and data on the parasite microbiome is, to the best of our...

Guts within guts: the microbiome of the intestinal helminth parasite Ascaris suum is derived but distinct from its host

Ankur Midha, Víctor Hugo Jarquín-Díaz, Friederike Ebner, Ulrike Löber, Rima Hayani, Arkadi Kundik, Alessio Cardilli, Emanuel Heitlinger, Sofia Kirke Forslund & Susanne Hartmann
Abstract Background Intestinal helminths are extremely prevalent among humans and animals. In particular, intestinal roundworms affect more than 1 billion people around the globe and are a major issue in animal husbandry. These pathogens live in intimate contact with the host gut microbiota and harbor bacteria within their own intestines. Knowledge of the bacterial host microbiome at the site of infection is limited, and data on the parasite microbiome is, to the best of our...

FKBP12 is a major regulator of ALK2 activity in multiple myeloma cells

Ingrid Quist-Løkken, Clara Andersson-Rusch, Martin Haugrud Kastnes, Jürgen Markus Kolos, Jerome Jatzlau, Hanne Hella, Oddrun Elise Olsen, Anders Sundan, Petra Knaus, Felix Hausch & Toril Holien
Abstract Background The immunophilin FKBP12 binds to TGF-β family type I receptors, including the BMP type I receptor ALK2. FKBP12 keeps the type I receptor in an inactive state and controls signaling activity. Removal of FKBP12 with drugs such as the FKBP-ligand FK506 enhances BMP activity in various cell types. In multiple myeloma cells, activation of SMAD1/5/8 leads to apoptosis. We hypothesized that removing FKBP12 from ALK2 in myeloma cells would potentiate BMP-induced ALK2-SMAD1/5/8 activity...

LINC00478-derived novel cytoplasmic lncRNA LacRNA stabilizes PHB2 and suppresses breast cancer metastasis via repressing MYC targets

Rong Guo, Yonghui Su, Qi Zhang, Bingqiu Xiu, Sheng Huang, Weiru Chi, Liyi Zhang, Lun Li, Jianjing Hou, Jia Wang, Jiajian Chen, Yayun Chi, Jingyan Xue & Jiong Wu
Abstract Background Metastasis is the predominant cause of mortality in patients with breast cancer. Long noncoding RNAs (lncRNAs) have been shown to drive important phenotypes in tumors, including invasion and metastasis. However, the lncRNAs involved in metastasis and their molecular and cellular mechanisms are still largely unknown. Methods The transcriptional and posttranscriptional processing of LINC00478-associated cytoplasmic RNA (LacRNA) was determined by RT-qPCR, semiquantitative PCR and 5′/3′ RACE. Paired-guide CRISPR/cas9 and CRISPR/dead-Cas9 systems was used to...

Registration Year

  • 2023
    24

Resource Types

  • Collection
    24

Affiliations

  • Freie Universität Berlin
    24
  • Charité - University Medicine Berlin
    8
  • Humboldt-Universität zu Berlin
    5
  • Chengdu University of Traditional Chinese Medicine
    4
  • Berlin Institute of Health at Charité - Universitätsmedizin Berlin
    4
  • Inner Mongolia Agricultural University
    3
  • Friedrich-Loeffler-Institut
    3
  • Max Delbrück Center for Molecular Medicine
    3
  • First Affiliated Hospital of Xiamen University
    2
  • Jiangsu Academy of Agricultural Sciences
    2