230 Works

A general method for calculating power for GEE analysis of complete and incomplete stepped wedge cluster randomized trials

Ying Zhang, John S Preisser, Elizabeth L Turner, Paul J Rathouz, Mark Toles & Fan Li
Stepped wedge designs have uni-directional crossovers at randomly assigned time points (steps) where clusters switch from control to intervention condition. Incomplete stepped wedge designs are increasingly used in cluster randomized trials of health care interventions and have periods without data collection due to logistical, resource and patient-centered considerations. The development of sample size formulae for stepped wedge trials has primarily focused on complete designs and continuous responses. Addressing this gap, a general, fast, non-simulation based...

Floral organ transcriptome in Camellia sasanqua provided insight into stamen petaloid

Menglong Fan, Xinlei Li, Ying Zhang, Si Wu, Zhixin Song, Hengfu Yin, Weixin Liu, Zhengqi Fan & Jiyuan Li
Abstract Background The cultivated Camellia sasanqua forms a divergent double flower pattern, and the stamen petaloid is a vital factor in the phenomenon. However, the regulation mechanism remains largely unclear. Results Here, a comprehensive comparative transcriptome analysis of the wild-type, “semi-double”, “peony double”, and “rose double” was performed. The cluster analysis of global gene expression level showed petal and stamen difficulty separable in double flower. The crucial pathway and genes related to double flower patterns...

Brewster metasurfaces for ultrabroadband reflectionless absorption at grazing incidence

huiying fan, Hongchen Chu, hao luo, Yun Lai, Lei Gao & jie luo
Previous reflectionless metasurfaces based on balanced electric and magnetic responses in engineered resonant meta-atoms become ineffective at oblique incident angles and usually have strong reflection at grazing incidence, where the impedance becomes near-zero or divergent. Here, by introducing the concept of anomalous generalized Brewster effect to metasurfaces, we demonstrate an exceptional resonance-free Brewster metasurface that exhibits ultra-broadband zero reflection at grazing incidence. The anomalous generalized Brewster effect is obtained via combining the mechanisms of the...

Relationship between body composition and bone mineral density in postmenopausal women with type 2 diabetes mellitus

Lei Gao, Ping Zhang, Yan Wang, Wei Zhang, Jian Zhao, Ying Liu, Jing Liu & Shaoqiang He
Abstract Background The aim of the study were to analyze the lumbar volumetric bone mineral density (BMD), fat distribution and changes of skeletal muscle with quantitative computed tomography (QCT) in postmenopausal women with type 2 diabetes mellitus (T2DM), and to evaluate the relationship between body composition and BMD. Methods One hundred seventy-seven postmenopausal women with T2DM and 136 postmenopausal women without diabetes were included in the study and were divided into two groups according to...

Low lipoprotein(a) concentration is associated with atrial fibrillation: a large retrospective cohort study

Junjie Tao, Xinlei Yang, Qingkai Qiu, Feng Gao, Wenchong Chen, Lijuan Hu, Yuan Xu, Yingping Yi, Hui Hu & Long Jiang
Abstract Background and aims The role of serum lipoprotein(a) [Lp(a)] levels in atrial fibrillation (AF) is still uncertain, especially in the Chinese population. Here, we aimed to elucidate the potential relationship between Lp(a) quantiles and AF. Methods All data were collected through inpatients with electronic health records from the Second Affiliated Hospital of Nanchang University, Jiangxi Province, China. The propensity score matching (PSM) method was used to match control and case groups. Interactions between AF,...

Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana

Bihan Chen, Feng Wang, Xi Xie, Huifan Liu, Dongjie Liu, Lukai Ma, Gengsheng Xiao & Qin Wang
Abstract Background Polyunsaturated fatty acid (PUFA) synthase is a multi-domain mega-enzyme that effectively synthesizes a series of PUFAs in marine microorganisms. The dehydratase (DH) domain of a PUFA synthase plays a crucial role in double bond positioning in fatty acids. Sequencing results of the coccolithophore Emiliania huxleyi (E. huxleyi, Eh) indicated that this species contains a PUFA synthase with multiple DH domains. Therefore, the current study, sought to define the functions of these DH domains...

Integrated analysis to identify the AC005154.6/hsa-miR-29c-3p/CCNL2 axis as a novel prognostic biomarker associated with immune infiltration in prostate cancer

Qinyu Li, Bingliang Chen, Guoda Song, Kai Zeng, Xin Chen, Jianping Miao, Xianglin Yuan, Jihong Liu, Zhihua Wang & Bo Liu
Abstract Background Prostate cancer (PCa) is currently the major malignancy in men. It is becoming increasingly clear that competitive endogenous RNA (ceRNA) regulation networks are important in a wide variety of cancers. Nevertheless, there is still much to learn about the biological functions of the ceRNA network in prostate cancer. Methods The ceRNA network was constructed using the "GDCRNATools" package. Based on survival analysis, we obtained AC005154.6/hsa-miR-29c-3p/CCNL2 for further analysis. The prognostic model based on...

LncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation

Xiaoying Wang, Qian Li, Siyu He, June Bai, Cui Ma, Lixin Zhang, Xiaoyu Guan, Hao Yuan, Yiying Li, Xiangrui Zhu, Jian Mei, Feng Gao & Daling Zhu
Abstract Background Pyroptosis is a form of programmed cell death involved in the pathophysiological progression of hypoxic pulmonary hypertension (HPH). Emerging evidence suggests that N6-methyladenosine (m6A)-modified transcripts of long noncoding RNAs (lncRNAs) are important regulators that participate in many diseases. However, whether m6A modified transcripts of lncRNAs can regulate pyroptosis in HPH progression remains unexplored. Methods The expression levels of FENDRR in hypoxic pulmonary artery endothelial cells (HPAECs) were detected by using quantitative real-time polymerase...

Pump-guided nonlinear film for phase conjugation

Pengyu Fu & Yue Li
It is known that phase conjugation technique is achieved by the nonlinearity of materials, and widely adopted in various applications, such as high-resolution imaging, signal amplification, and target detecting. Here, we have proposed a field-enhancement method for the degenerate four wave mixing (FWM) for phase conjugation purpose. In this method, a thin metasurface with nonlinear property is utilized to confine and guide the pumps, achieving the enhanced FWM within a flexible structure. Compared to existing...

Associations between inflammatory bowel disease, social isolation, and mortality: evidence from a longitudinal cohort study

Jie Chen, Jiawei Geng, Jiayi Wang, Zhenhua Wu, Tian Fu, Yuhao Sun, Xuejie Chen, Xiaoyan Wang & Therese Hesketh
Background:Social well-being of patients with inflammatory bowel disease (IBD) is garnering increased attention; however, the impact of social isolation remained poorly understood.Objectives:We investigated the joint association of social isolation and IBD with premature deaths to articulate the profound impact of social isolation in IBD prognosis.Design:Longitudinal cohort study.Methods:We leveraged data of 486,014 participants from UK Biobank (including 5791 with IBD), the mean follow-up was 11.84 years. Diagnoses of IBD and its subtypes of Crohn’s disease (CD)...

Transcriptome analysis of intestine from alk-SMase knockout mice reveals the effect of alk-SMase

Jiang Zhu, Lingqi Wang, Zhongwu Guo, Tao Zhang & Ping Zhang
Abstract Objective Intestinal alkaline sphingomyelinase (alk-SMase) generates ceramide and inactivates platelet-activating factor associated with digestion and inhibition of cancer. There is few study to analyze the correlated function and characterize the genes related to alk-SMase comprehensively. We characterised transcriptome landscapes of intestine tissues from alk-SMase knockout (KO) mice aiming to identify novel associated genes and research targets. Methods We performed the high-resolution RNA sequencing of alk-SMase KO mice and compared them to wild type (WT)...

Comprehensive analysis of the differences between left- and right-side colorectal cancer and respective prognostic prediction

Mengye Niu, Chengyang Chen, Xian Gao, Yi Guo, Bingzhou Zhang, Xin Wang, Shihao Chen, Xupeng Niu, Chao Zhang, Like Li, Zhongxin Li, Zengren Zhao & Xia Jiang
Abstract Background Previous studies have reported that the tumor heterogeneity and complex oncogenic mechanisms of proximal and distal colon cancer (CRC) are divergent. Therefore, we aim to analyze the differences between left-sided CRC (L_cancer) and right-sided CRC (R_cancer), as well as constructing respective nomograms. Methods We enrolled 335 colon cancer patients (146 L_cancer patients and 189 R_cancer patients) from The Cancer Genome Atlas (TCGA) data sets, and 102 pairs of color cancer tissue and adjacent...

SWI/SNF complex gene variations are associated with a higher tumor mutational burden and a better response to immune checkpoint inhibitor treatment: a pan-cancer analysis of next-generation sequencing data corresponding to 4591 cases

Yue Li, Xinhua Yang, Weijie Zhu, Yuxia Xu, Jiangjun Ma, Caiyun He & Fang Wang
Abstract Background Genes related to the SWItch/sucrose nonfermentable (SWI/SNF) chromatin remodeling complex are frequently mutated across cancers. SWI/SNF-mutant tumors are vulnerable to synthetic lethal inhibitors. However, the landscape of SWI/SNF mutations and their associations with tumor mutational burden (TMB), microsatellite instability (MSI) status, and response to immune checkpoint inhibitors (ICIs) have not been elucidated in large real-world Chinese patient cohorts. Methods The mutational rates and variation types of six SWI/SNF complex genes (ARID1A, ARID1B, ARID2,...

Echinococcus granulosus sensu stricto and antigen B may decrease inflammatory bowel disease through regulation of M1/2 polarization

Jianling Bao, Wenjing Qi, Chang Sun, Mengxiao Tian, Hongjie Jiao, Gang Guo, Baoping Guo, Yuan Ren, Huajun Zheng, Yuezhu Wang, Mei Yan, Zhaoxia Zhang, Donald P. McManus, Jun Li & Wenbao Zhang
Abstract Background Inflammatory bowel disease (IBD) is a chronic idiopathic disease characterized by inflammation-related epithelial barrier damage in the intestinal tract. Helminth infection reduces autoimmune disease symptoms through regulation of inflammatory responses based on hygiene theory. However, the underlying mechanisms remain unclear. Methods BALB/c mice were infected with microcysts of E. granulosus sensu stricto and drank water containing 3.5% dextran sodium sulfate (DSS) at the 100th day post-infection. After 7 days of drinking DSS, the...

Predictive Value of Adenoid-Nasopharyngeal Ratio in the Diagnosis of Secretory Otitis Media

Ping Li, Tao Li, Liang Yu, Aiping Chen, Yisha Wu, Yuzhu Wan & Li Shi
ObjectivesTo explore the associated factors of otitis media with effusion (OME) and analyze the diagnostic value of the adenoid-nasopharyngeal (A/N) ratio to OME.MethodsPatients with adenoid hypertrophy (AH) recently in 2 years were collected, including sex, age, duration, with/without rhinosinusitis, and examination results, including Cone Beam Computerized Tomography (CBCT) sinus imaging, tympanometry, pure tone audiometry (PTA), blood test, and allergen detection. According to Liden/Jerger’s classification, the patients were divided into two groups: Type B and type...

The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells

Qi Wang, Yongqiang Qi, Fei Xiong, Da Wang, Bing Wang & Yongjun Chen
Abstract Background Intrahepatic cholangiocarcinoma (ICC), originating from the bile ducts, is the second most common primary liver malignancy, and its incidence has recently increased. H2A.Z, a highly conserved H2A variant, is emerging as a key regulatory molecule in cancer. However, its underlying mechanism of action in ICC cells remains unclear. Methods Here, we examined the expression of H2A.Z and SFRP1 in normal intrahepatic cholangiocytes, ICC cell lines, ICC tissue microarrays, and fresh specimens. The correlations...

Proteomic profiling of circulating plasma exosomes reveals novel biomarkers of Alzheimer’s disease

Huimin Cai, Yana Pang, Qi Wang, Wei Qin, Cuibai Wei, Ying Li, Tingting Li, Fangyu Li, Qigeng Wang, Yan Li, Yiping Wei & Longfei Jia
Abstract Background Neuronal- and astrocyte-derived exosomes have been identified as an optimal source for screening biomarkers for Alzheimer’s disease (AD). However, few studies focus on the bulk exosome population isolated from plasma of AD. This study investigated whether proteins in bulk exosomes can aid in the diagnosis of AD. Methods The plasma exosomes were collected by ultracentrifuge. Protein samples were extracted from exosomes. Cerebrospinal fluid levels of amyloid β (Aβ)42 and phosphorylated tau (P-tau)181 were...

Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in septic mice

Yan Li, Zhaoying Li, Fujuan He, Chenguang Qin, Rui Fan, Fangxiang Zhang & Bin Wang
Background:Sepsis is defined as organ dysfunction caused by an uncontrolled response to infection and is followed by a high incidence of cognitive dysfunction, which can severely affect patients’ quality of life. Previous studies have suggested that electroacupuncture (EA) is protective against sepsis-associated cognitive dysfunction and that pyroptosis plays a vital role in cognitive function. The aim of this study was to investigate the effect of EA on cognition and neuronal pyroptosis in a mouse model...

Evaluate the immune-related eRNA models and signature score to predict the response to immunotherapy in thyroid carcinoma

Pu Wu, Jinyuan Shi, Zhiyuan Wang, Wei Sun & Hao Zhang
Abstract Background The functional alterations of eRNAs have been reported to be correlated with tumorigenesis. However, the roles of eRNAs in thyroid cancer (THCA) remain still unclear. This study aimed to construct an immune-related eRNA prognostic signature that could effectively predict the survival and prognosis for THCA. Methods The Weighted Gene Co-Expression Network Analysis (WGCNA) was performed to identify THCA-specific immune-related hub genes and immune-related eRNAs were obtained using Pearson correlation analysis. Univariate and least...

Evidence-based expert consensus on the management of primary central nervous system lymphoma in China

Tong Chen, Yuanbo Liu, Yang Wang, Qing Chang, Jinsong Wu, Zhiliang Wang, Daoying Geng, Jin-Tai Yu, Yuan Li, Xiao-Qiu Li, Hong Chen, Dongxiao Zhuang, Jianyong Li, Bin Wang, Tao Jiang, Lanting Lyu, Yuqin Song, Xiaoguang Qiu, Wenbin Li, Song Lin, Xinghu Zhang, Dehong Lu, Junqiang Lei, Yaolong Chen & Ying Mao
Abstract Primary central nervous system lymphoma (PCNSL) is a type of central nervous system restricted non-Hodgkin lymphoma, whose histopathological diagnosis is majorly large B cell lymphoma. To provide specific, evidence-based recommendations for medical professionals and to promote more standardized, effective and safe treatment for patients with PCNSL, a panel of experts from the Chinese Neurosurgical Society of the Chinese Medical Association and the Society of Hematological Malignancies of the Chinese Anti-Cancer Association jointly developed an...

Antivirus isoindolinone alkaloids with rare oxocyclopenta[f]isoindole frameworks isolated from the stems of flue cured tobacco

Qiu-Fen Hu, Yue-Yu Ma, Hua-Yin Liu, Jia-Meng Dai, Feng-Xian Yang, Jian-Duo Zhang, Jin Wang, Xue-Mei Li, Xin Liu, Jing Li, Yin-Ke Li, Wei-Guang Wang, Min Zhou & Guang-Yu Yang
Abstract Background Since Nicotiana tabacum (tobacco) has important significance to humans for their medicinal uses, to find antivirus activities inhibitors from tobacco, increase its medicinal value, and comprehensive utilization of its by-products, our group had investigated the chemical constituents of the stems of Y-202, a cultivar of tobacco which high resistance to tobacco mosaic virus (TMV). Results Four new isoindolinone-type alkaloids, nicoisoindoles A–D (1–4), along with four known isoindole derivatives (5–8) were isolated. Compounds 1–4...

Targeted OUM1/PTPRZ1 silencing and synergetic CDT/enhanced chemical therapy toward uveal melanoma based on a dual-modal imaging-guided manganese metal–organic framework nanoparticles

Yue Li, Fang Li, Hui Pan, Xiaolin Huang, Jie Yu, Xueru Liu, Qinghao Zhang, Caiwen Xiao, He Zhang & Leilei Zhang
Abstract Metastasis and chemical resistance are the most serious problems in the treatment of highly aggressive uveal melanoma (UM). The newly identified lncRNA OUM1 is overexpressed in UM, functions as a catalyst and regulates protein tyrosine phosphatase (PTP) activity by binding to PTP receptor type Z1 (PTPRZ1), which plays an important role in cell proliferation, metastasis and chemotherapy resistance in the UM microenvironment. Hence, siRNAs that selectively knocking down the lncRNA OUM1 (siOUM1) and its...

Deciphering chloramphenicol biotransformation mechanisms and microbial interactions via integrated multi-omics and cultivation-dependent approaches

Jiayu Zhang, Xiaoyan Li, Uli Klümper, Huaxin Lei, Thomas U. Berendonk, Fangliang Guo, Ke Yu, Chao Yang & Bing Li
Abstract Background As a widely used broad-spectrum antibiotic, chloramphenicol is prone to be released into environments, thus resulting in the disturbance of ecosystem stability as well as the emergence of antibiotic resistance genes. Microbes play a vital role in the decomposition of chloramphenicol in the environment, and the biotransformation processes are especially dependent on synergistic interactions and metabolite exchanges among microbes. Herein, the comprehensive chloramphenicol biotransformation pathway, key metabolic enzymes, and interspecies interactions in an...

OCT based four-dimensional cardiac imaging of living chick embryo using impedance signal as the gating for post-acquisition synchronization

Yushu Ma, Chuanxi Li, Huiwen Jiang, Yuqian Zhao, JIAN LIU, Yao Yu, Yi Wang, Wenbo Shi & Zhenhe Ma
Four-dimensional (4-D) imaging of embryonic heart is valuable for the study of in vivo cardiac morphology and function during development. Optical coherence tomography (OCT) is a non-invasive imaging modality with high spatial resolution suitable for early embryonic heart imaging. However, most commonly used OCT systems cannot provide direct 4-D imaging due to acquisition speed limitations. We proposed a retrospective gating 4-D reconstruction method based on spectral domain OCT. A special circuit was designed to measure...

Complete chloroplast genomes of Sorbus sensu stricto (Rosaceae): comparative analyses and phylogenetic relationships

Chenqian Tang, Xin Chen, Yunfei Deng, Liyang Geng, Jianhui Ma & Xueyan Wei
Abstract Background Sorbus sensu stricto (Sorbus s.s.) is a genus with important economical values because of its beautiful leaves, and flowers and especially the colorful fruits. It belongs to the tribe Maleae of the family Rosaceae, and comprises about 90 species mainly distributed in China. There is on-going dispute about its infrageneric classification and species delimitation as the species are morphologically similar. With the aim of shedding light on the circumscription of taxa within the...

Registration Year

  • 2022

Resource Types

  • Collection


  • Second Hospital of Anhui Medical University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Sichuan University
  • Fudan University
  • Shanghai Jiao Tong University
  • Zhejiang University
  • Huazhong University of Science and Technology
  • Sun Yat-sen University
  • Tongji University
  • Shandong University