IntroductionTo validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6).MethodsAll hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20–25 mmHg to achieve...

A large amount of literature demonstrates that social behaviour can be triggered by environmental cues. A long-standing debate involves the question of whether such stimuli trigger behaviour directly (i.e. habits) or whether these effects mediate goals. As studies on automatic goal pursuit typically use real-world cues that are already associated with the behaviour and potentially the goal, it is impossible to make strong claims about the nature of the effects. In the present paper, we...

A large amount of literature demonstrates that social behaviour can be triggered by environmental cues. A long-standing debate involves the question of whether such stimuli trigger behaviour directly (i.e. habits) or whether these effects mediate goals. As studies on automatic goal pursuit typically use real-world cues that are already associated with the behaviour and potentially the goal, it is impossible to make strong claims about the nature of the effects. In the present paper, we...

We report on a spectral splitting effect of the cavity-less lasing emission of nitrogen ions at 391.4 nm pumped by the 800 nm femtosecond laser pulses. It was found that with the increase of the nitrogen gas pressure and pump pulse energy, both R and P branches experience spectral splitting. With an external injected seeding pulse, similar split spectral line is observed for the amplified emission. In contrast, for the fluorescence radiation, no such spectral...

The use of lightweight sheets such as carbon fiber reinforced composites (CFRP), magnesium alloys, and aluminum alloys is of great significance to realize the lightweight of automobiles. However, the differences between the sheets make the joining processes of dissimilar sheets a great challenge. A pre-holed screw-drilling riveting (PH-SDR) process is proposed based on a study of existing joining processes, the rivet with threads is riveted into the sheets by rotating, and the effective joining between...

The use of lightweight sheets such as carbon fiber reinforced composites (CFRP), magnesium alloys, and aluminum alloys is of great significance to realize the lightweight of automobiles. However, the differences between the sheets make the joining processes of dissimilar sheets a great challenge. A pre-holed screw-drilling riveting (PH-SDR) process is proposed based on a study of existing joining processes, the rivet with threads is riveted into the sheets by rotating, and the effective joining between...

IntroductionTo validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6).MethodsAll hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20–25 mmHg to achieve...

The tricarboxylic acid (TCA) cycle, is the central pathway of energy production in eukaryotic cells and plays a key part in aerobic respiration throughout all kingdoms of life. One of the pivotal enzymes in this cycle is 2-oxoglutarate dehydrogenase complex (OGDHC), which generates NADH by oxidative decarboxylation of 2-oxoglutarate to succinyl-CoA. OGDHC is a megadalton protein complex originally thought to be assembled just from three catalytically active subunits (E1o, E2o, E3). In fungi and animals,...

The tricarboxylic acid (TCA) cycle, is the central pathway of energy production in eukaryotic cells and plays a key part in aerobic respiration throughout all kingdoms of life. One of the pivotal enzymes in this cycle is 2-oxoglutarate dehydrogenase complex (OGDHC), which generates NADH by oxidative decarboxylation of 2-oxoglutarate to succinyl-CoA. OGDHC is a megadalton protein complex originally thought to be assembled just from three catalytically active subunits (E1o, E2o, E3). In fungi and animals,...

AimsMultinomial logistic regression models allow one to predict the risk of a categorical outcome with > 2 categories. When developing such a model, researchers should ensure the number of participants (n) is appropriate relative to the number of events (Ek) and the number of predictor parameters (pk) for each category k. We propose three criteria to determine the minimum n required in light of existing criteria developed for binary outcomes.Proposed criteriaThe first criterion aims to...

Objectives:We identified factors associated with healthcare costs and health-related quality of life (HRQoL) of multimorbid older adults with polypharmacy.Methods:Using data from the OPERAM (OPtimising thERapy to prevent Avoidable hospital admissions in the Multimorbid older people) trial, we described the magnitude and composition of healthcare costs, and time trends of HRQoL, during 1-year after an acute-care hospitalization. We performed a cluster analysis to identify groups with different cost and HRQoL trends. Using multilevel models, we also...

We report on a spectral splitting effect of the cavity-less lasing emission of nitrogen ions at 391.4 nm pumped by the 800 nm femtosecond laser pulses. It was found that with the increase of the nitrogen gas pressure and pump pulse energy, both R and P branches experience spectral splitting. With an external injected seeding pulse, similar split spectral line is observed for the amplified emission. In contrast, for the fluorescence radiation, no such spectral...

Objectives:We identified factors associated with healthcare costs and health-related quality of life (HRQoL) of multimorbid older adults with polypharmacy.Methods:Using data from the OPERAM (OPtimising thERapy to prevent Avoidable hospital admissions in the Multimorbid older people) trial, we described the magnitude and composition of healthcare costs, and time trends of HRQoL, during 1-year after an acute-care hospitalization. We performed a cluster analysis to identify groups with different cost and HRQoL trends. Using multilevel models, we also...

AimsMultinomial logistic regression models allow one to predict the risk of a categorical outcome with > 2 categories. When developing such a model, researchers should ensure the number of participants (n) is appropriate relative to the number of events (Ek) and the number of predictor parameters (pk) for each category k. We propose three criteria to determine the minimum n required in light of existing criteria developed for binary outcomes.Proposed criteriaThe first criterion aims to...