213 Works

Nonstructural proteins 2B and 4A of Tembusu virus induce complete autophagy to promote viral multiplication in vitro

Wangyang Tan, Senzhao Zhang, Yu He, Zhen Wu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Qun Gao, Di Sun, Bin Tian, Shun Chen & Anchun Cheng
Abstract Tembusu virus (TMUV) is an emerging flavivirus that has broken out in different regions of China. TMUV infection has been reported to induce autophagy in duck embryo fibroblast cells. However, the molecular mechanisms underlying this autophagy induction remain unclear. Here, we explored the interactions between autophagy and TMUV and the effects of the structural and nonstructural proteins of TMUV on autophagy in vitro. Among our results, TMUV infection enhanced autophagy to facilitate viral replication...

Echocardiographic assessment of Xenopus tropicalis heart regeneration

Luocheng Lv, Weimin Guo, Wei Guan, Yilin Chen, Ruijin Huang, Ziqiang Yuan, Qin Pu, Shanshan Feng, Xin Zheng, Yanmei Li, Luanjuan Xiao, Hui Zhao, Xufeng Qi & Dongqing Cai
Abstract Background Recently, it was reported that the adult X. tropicalis heart can regenerate in a nearly scar-free manner after injury via apical resection. Thus, a cardiac regeneration model in adult X. tropicalis provides a powerful tool for recapitulating a perfect regeneration phenomenon, elucidating the underlying molecular mechanisms of cardiac regeneration in an adult heart, and developing an interventional strategy for the improvement in the regeneration of an adult heart, which may be more applicable...

RUNX3 mediates keloid fibroblast proliferation through deacetylation of EZH2 by SIRT1

Hanye Liu, Guanghai Yan, Li Li, Dandan Wang, Yu Wang, Shan Jin, Zhehu Jin, Liangchang Li & Lianhua Zhu
Abstract Background Keloid is a benign proliferative fibrous disease featured by excessive fibroblast proliferation after skin injury. However, the mechanism of abnormal cell proliferation is still unclear. Herein, we investigated the mechanism of abnormal proliferation in keloids involving Sirtuin 1(SIRT1)/ Zeste Homolog 2 (EZH2)/ Runt-related transcription factor 3 (RUNX3). Methods HE staining was used to observe the histopathological changes. Western blot was performed to detect SIRT1/EZH2/RUNX3 and cell cycle related proteins. RT-PCR detected EZH2 mRNA....

Multiple founding paternal lineages inferred from the newly-developed 639-plex Y-SNP panel suggested the complex admixture and migration history of Chinese people

Guanglin He, Mengge Wang, Lei Miao, Jing Chen, Jie Zhao, Qiuxia Sun, Shuhan Duan, Zhiyong Wang, Xiaofei Xu, Yuntao Sun, Yan Liu, Jing Liu, Zheng Wang, Lanhai Wei, Chao Liu, Jian Ye & Le Wang
Abstract Background Non-recombining regions of the Y-chromosome recorded the evolutionary traces of male human populations and are inherited haplotype-dependently and male-specifically. Recent whole Y-chromosome sequencing studies have identified previously unrecognized population divergence, expansion and admixture processes, which promotes a better understanding and application of the observed patterns of Y-chromosome genetic diversity. Results Here, we developed one highest-resolution Y-chromosome single nucleotide polymorphism (Y-SNP) panel targeted for uniparental genealogy reconstruction and paternal biogeographical ancestry inference, which included...

Reflection on the teaching of student-centred formative assessment in medical curricula: an investigation from the perspective of medical students

Tianjiao Ma, Yin Li, Hua Yuan, Feng Li, Shujuan Yang, Yongzhi Zhan, Jiannan Yao & Dongmei Mu
Abstract Background Formative assessment (FA) is becoming increasingly common in higher education, although the teaching practice of student-centred FA in medical curricula is still very limited. In addition, there is a lack of theoretical and pedagogical practice studies observing FA from medical students’ perspectives. The aim of this study is to explore and understand ways to improve student-centred FA, and to provide a practical framework for the future construction of an FA index system in...

Transcriptome analysis of barley (Hordeum vulgare L.) under waterlogging stress, and overexpression of the HvADH4 gene confers waterlogging tolerance in transgenic Arabidopsis

Haiye Luan, Hongtao Li, Yu Li, Changyu Chen, Shufeng Li, Yu Wang, Ju Yang, Meng Xu, Huiquan Shen, Hailong Qiao & Jun Wang
Abstract Background Waterlogging is one of the major abiotic stresses in barley and greatly reduces grain yield and quality. To explore the mechanism controlling waterlogging tolerance in barley, physiological, anatomical and transcriptional analyses were performed in two contrasting barley varieties, viz. Franklin (susceptible) and TX9425 (tolerant). Results Compared to Franklin, TX9425 had more adventitious roots and aerenchymas and higher antioxidant enzyme activities. A total of 3064 and 5693 differentially expressed genes (DEGs) were identified in...

SPOCK1 and POSTN are valuable prognostic biomarkers and correlate with tumor immune infiltrates in colorectal cancer

Caiqin Gan, Mengting Li, Yuanyuan Lu, Ganjing Peng, Wenjie Li, Haizhou Wang, Yanan Peng, Qian Hu, Wanhui Wei, Fan Wang, Lan Liu & Qiu Zhao
Abstract Background Immune cells and stromal cells in the tumor microenvironment play a vital role in the progression of colorectal cancer (CRC). The study aimed to screen valuable prognostic biomarkers in CRC based on stromal and immune scores. Method The ESTIMATE algorithm was used to calculate the immune and stromal scores of CRC samples in TCGA. Then samples were divided into high and low score groups based on the median value of the scores. Differentially...

SPOCK1 and POSTN are valuable prognostic biomarkers and correlate with tumor immune infiltrates in colorectal cancer

Caiqin Gan, Mengting Li, Yuanyuan Lu, Ganjing Peng, Wenjie Li, Haizhou Wang, Yanan Peng, Qian Hu, Wanhui Wei, Fan Wang, Lan Liu & Qiu Zhao
Abstract Background Immune cells and stromal cells in the tumor microenvironment play a vital role in the progression of colorectal cancer (CRC). The study aimed to screen valuable prognostic biomarkers in CRC based on stromal and immune scores. Method The ESTIMATE algorithm was used to calculate the immune and stromal scores of CRC samples in TCGA. Then samples were divided into high and low score groups based on the median value of the scores. Differentially...

Association between human blood metabolome and the risk of breast cancer

Yu Wang, Fanghua Liu, Lulu Sun, Yiming Jia, Pinni Yang, Daoxia Guo, Mengyao Shi, Aili Wang, Guo-Chong Chen, Yonghong Zhang & Zhengbao Zhu
Abstract Background Breast cancer is the most common cancer among women with limited treatment options. To identify promising drug targets for breast cancer, we conducted a systematical Mendelian randomization (MR) study to screen blood metabolome for potential causal mediators of breast cancer and further predict target-mediated side effects. Methods We selected 112 unique blood metabolites from 3 large-scale European ancestry-based genome-wide association studies (GWASs) with a total of 147,827 participants. Breast cancer data were obtained...

Conservatively transmitted alleles of key agronomic genes provide insights into the genetic basis of founder parents in bread wheat (Triticum aestivum L.)

Chang Li, Lei Zhuang, Tian Li, Jian Hou, Hongxia Liu, Chao Jian, Huifang Li, Jing Zhao, Yunchuan Liu, Wei Xi, Pingan Hao, Shujuan Liu, Xuemei Si, Xiaolu Wang, Xueyong Zhang & Chenyang Hao
Abstract Background Founder parents play extremely important roles in wheat breeding. Studies into the genetic basis of founder parents and the transmission rules of favorable alleles are of great significance in improving agronomically important traits in wheat. Results Here, a total of 366 founder parents, widely grown cultivars, and derivatives of four representative founder parents were genotyped based on efficient kompetitive allele-specific PCR (KASP) markers in 87 agronomically important genes controlling yield, quality, adaptability, and...

Genomic and evolutionary relationships among wild and cultivated blueberry species

Byron R. Manzanero, Krishnanand P. Kulkarni, Nicholi Vorsa, Umesh K. Reddy, Purushothaman Natarajan, Sathya Elavarthi, Massimo Iorizzo & Kalpalatha Melmaiee
Abstract Background Blueberries (Vaccinium section Cyanococcus) are an economically important fruit crop in the United States. Understanding genetic structure and relationships in blueberries is essential to advance the genetic improvement of horticulturally important traits. In the present study, we investigated the genomic and evolutionary relationships in 195 blueberry accessions from five species (comprising 33 V. corymbosum, 14 V. boreale, 81 V. darrowii, 29 V. myrsinites, and 38 V. tenellum) using single nucleotide polymorphisms (SNPs) mined...

Comparing a PD-L1 inhibitor plus chemotherapy to chemotherapy alone in neoadjuvant therapy for locally advanced ESCC: a randomized Phase II clinical trial

Yong Li, Aiping Zhou, Shuoyan Liu, Ming He, Keneng Chen, Ziqiang Tian, Yin Li, Jianjun Qin, Zhen Wang, Haiquan Chen, Hui Tian, Yue Yu, Wang Qu, Liyan Xue, Shun He, Shuhang Wang, Fenglong Bie, Guangyu Bai, Bolun Zhou, Zhaoyang Yang, Huiyao Huang, Yan Fang, Benjamin Li, Xiangrong Dai, Shugeng Gao … & Jie He
Abstract Background A Phase II study was undertaken to evaluate the safety and efficacy of the neoadjuvant socazolimab, a novel PD-L1 inhibitor, in combination with nab-paclitaxel and cisplatin for locally advanced esophageal squamous cell carcinoma (ESCC). Methods Sixty-four patients were randomly divided between the Socazolimab + nab-paclitaxel + cisplatin (TP) arm (n = 32) and the control arm (n = 32), receiving either socazolimab (5 mg/kg intravenously (IV), day 1) or a placebo with nab-paclitaxel...

Regional anesthesia did not improve postoperative long-term survival of tumor patients: a systematic review and meta-analysis of randomized controlled trials

Tao Li, Xiangrui Meng, Di Wang, Qiang Wang, Jiahai Ma & Zhao Dai
Abstract Objective Experimental research and clinical trials have reported a positive effect of regional anesthesia (RA) on prognosis of cancers. We systematically reviewed the efficacy of RA on recurrence-free survival (RFS) and overall survival (OS) after oncology surgeries. Methods PubMed, Cochrane library, and Embase were searched from inception to June 20, 2022 for RCTs in which any form of RA was initiated perioperatively. Time-to-event data (hazard ratio (HR)) were extracted independently and in duplicate. The...

Sexual needs of people with schizophrenia: a descriptive phenomenological study

Jin-wei Yang, Kai Yu, Xiao-qing Wang, Yu Wang, Chen-Chen Zhang, Rui Ma, Hong Yu & Yu-qiu Zhou
Abstract Background Sexual health is one of the main areas of health and basic human rights which has been paid less attention in schizophrenia. Most studies have focused on sexual dysfunction rather than the sexual needs of people with schizophrenia. This study explores the sexual needs of people with schizophrenia and identify factors hindering sexual activities. Methods We carried out a qualitative study using a descriptive phenomenological approach. Data were collected at a psychiatric hospital...

Canine distemper virus N protein induces autophagy to facilitate viral replication

Fei Chen, Zijing Guo, Rui Zhang, Zhixiong Zhang, Bo Hu, Ling Bai, Shuaiyang Zhao, Yongshu Wu, Zhidong Zhang & Yanmin Li
Abstract Background Canine distemper virus (CDV) is one of the most contagious and lethal viruses known to the Canidae, with a very broad and expanding host range. Autophagy serves as a fundamental stabilizing response against pathogens, but some viruses have been able to evade or exploit it for their replication. However, the effect of autophagy mechanisms on CDV infection is still unclear. Results In the present study, autophagy was induced in CDV-infected Vero cells as...

Pathobionts from chemically disrupted gut microbiota induce insulin-dependent diabetes in mice

Xin Yang, Zhiyi Wang, Junling Niu, Rui Zhai, Xinhe Xue, Guojun Wu, Yuanyuan Fang, Guangxun Meng, Huijuan Yuan, Liping Zhao & Chenhong Zhang
Abstract Background Dysbiotic gut microbiome, genetically predisposed or chemically disrupted, has been linked with insulin-dependent diabetes (IDD) including autoimmune type 1 diabetes (T1D) in both humans and animal models. However, specific IDD-inducing gut bacteria remain to be identified and their casual role in disease development demonstrated via experiments that can fulfill Koch’s postulates. Results Here, we show that novel gut pathobionts in the Muribaculaceae family, enriched by a low-dose dextran sulfate sodium (DSS) treatment, translocated...

Cluster analysis of COVID-19 recovery center patients at a clinic in Boston, MA 2021–2022: impact on strategies for access and personalized care

Ann-Marcia C. Tukpah, Jhillika Patel, Beret Amundson, Miguel Linares, Meera Sury, Julie Sullivan, Tajmah Jocelyn, Brenda Kissane, Gerald Weinhouse, Nancy Lange-Vaidya, Daniela Lamas, Khalid Ismail, Chandan Pavuluri, Michael H. Cho, Elizabeth B. Gay & Matthew Moll
Abstract Background There are known disparities in COVID-19 resource utilization that may persist during the recovery period for some patients. We sought to define subpopulations of patients seeking COVID-19 recovery care in terms of symptom reporting and care utilization to better personalize their care and to identify ways to improve access to subspecialty care. Methods Prospective study of adult patients with prior COVID-19 infection seen in an ambulatory COVID-19 recovery center (CRC) in Boston, Massachusetts...

Analysis and verification of the circRNA regulatory network RNO_CIRCpedia_ 4214/RNO-miR-667-5p/Msr1 axis as a potential ceRNA promoting macrophage M2-like polarization in spinal cord injury

Jian Cao, Chongzhi Pan, Jian Zhang, Qi Chen, Tao Li, Dingwen He & Xigao Cheng
Abstract Background CircRNAs are involved in the pathogenesis of several central nervous system diseases. However, their functions and mechanisms in spinal cord injury (SCI) are still unclear. Therefore, the purpose of this study was to evaluate circRNA and mRNA expression profiles in the pathological setting of SCI and to predict the potential function of circRNA through bioinformatics. Methods A microarray-based approach was used for the simultaneous measurement of circRNAs and mRNAs, together with qPCR, fluorescence...

TMED3 promotes the development of malignant melanoma by targeting CDCA8 and regulating PI3K/Akt pathway

Xianling Guo, Xiaolan Yin, Yu Xu, Liang Li, Min Yuan, Huaxin Zhao, Yuxiong Jiang, Xiujuan Shi, Hongda Bi, Yeqiang Liu, Yong Chen & Qing Xu
Abstract Background Transmembrane emp24 domain containing (TMED) proteins are known to play pivotal roles in normal development, but have been reported to be implicated in pancreatic disease, immune system disorders, and cancers. As far as TMED3 is concerned, its roles in cancers are controversial. However, evidence describing TMED3 in the context of malignant melanoma (MM) is scarce. Results In this study, we characterized the functional significance of TMED3 in MM and identified TMED3 as a...

Self-registration of constant-step rotating Mueller matrix polarimeters

Tao Xuan, Haoyu Zhai, Honghui He, Chao He, Liu Shaoxiong & Hui Ma
Time-division framework is commonly used in Mueller matrix polarimeters (MPs), which takes extra numbers of images at the same position in an acquisition sequence. In this Letter, we utilize measurement redundancy to raise a unique loss function which can reflect and evaluate the degree of mis-registration of Mueller matrix (MM) polarimetric images. Further, we demonstrate that the constant-step rotating MPs have a self-registration loss function free from systematic errors. Based on this property, we propose...

Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden

Vedad Delic, Joshua H. Karp, Maynard Guzman, Gabriel R. Arismendi, Katherine J. Stalnaker, Julia A. Burton, Kathleen E. Murray, Joshua P. Stamos, Kevin D. Beck, Arpine Sokratian, Andrew B. West & Bruce A. Citron
Abstract Population studies have shown that traumatic brain injury (TBI) is associated with an increased risk for Parkinson’s disease (PD) and among U.S. Veterans with a history of TBI this risk is 56% higher. The most common type of TBI is mild (mTBI) and often occurs repeatedly among athletes, military personnel, and victims of domestic violence. PD is classically characterized by deficits in fine motor movement control resulting from progressive neurodegeneration of dopaminergic neurons in...

A genetic map of the chromatin regulators to drug response in cancer cells

Bo Chen, Pengfei Li, Mingyue Liu, Kaidong Liu, Min Zou, Yiding Geng, Shuping Zhuang, Huanhuan Xu, Linzhu Wang, Tingting Chen, Yawei Li, Zhangxiang Zhao, Lishuang Qi & Yunyan Gu
Abstract Background Diverse drug vulnerabilities owing to the Chromatin regulators (CRs) genetic interaction across various cancers, but the identification of CRs genetic interaction remains challenging. Methods In order to provide a global view of the CRs genetic interaction in cancer cells, we developed a method to identify potential drug response-related CRs genetic interactions for specific cancer types by integrating the screen of CRISPR-Cas9 and pharmacogenomic response datasets. Results Totally, 625 drug response-related CRs synthetic lethality...

Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Yongbin Li, Rui Yang, Max M. Häggblom, Mengyan Li, Lifang Guo, Baoqin Li, Max Kolton, Zhiguo Cao, Mohsen Solemani, Zheng Chen, Zhimin Xu, Wenlong Gao, Bei Yan & Weimin Sun
Abstract Background Phytoremediation is a potentially cost-effective way to remediate highly contaminated mine tailing sites. However, nutrient limitations, especially the deficiency of nitrogen (N), can hinder the growth of plants and impair the phytoremediation of mine tailings. Nevertheless, pioneer plants can successfully colonize mine tailings and exhibit potential for tailing phytoremediation. Diazotrophs, especially diazotrophic endophytes, can promote the growth of their host plants. This was tested in a mine-tailing habitat by a combination of field...

Predictive Value of Adenoid-Nasopharyngeal Ratio in the Diagnosis of Secretory Otitis Media

Ping Li, Tao Li, Liang Yu, Aiping Chen, Yisha Wu, Yuzhu Wan & Li Shi
ObjectivesTo explore the associated factors of otitis media with effusion (OME) and analyze the diagnostic value of the adenoid-nasopharyngeal (A/N) ratio to OME.MethodsPatients with adenoid hypertrophy (AH) recently in 2 years were collected, including sex, age, duration, with/without rhinosinusitis, and examination results, including Cone Beam Computerized Tomography (CBCT) sinus imaging, tympanometry, pure tone audiometry (PTA), blood test, and allergen detection. According to Liden/Jerger’s classification, the patients were divided into two groups: Type B and type...

MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer

Pushuai Wen, Huade Wang, Yi Li, Xinyao Sui, Zhijuan Hou, Xiaoyan Guo, Wanying Xue, Dahua Liu, Yu Wang & Jing Gao
Abstract Background Molecule interacting with CasL-like protein 2 (MICALL2) is believed to regulate cytoskeleton dynamics, tight junction formation, and neurite outgrowth. However, its biological role and the underlying mechanism in colorectal cancer (CRC) remain largely elusive. Methods qRT-PCR, Western blotting and immunohistochemistry assays were used to detect the expression levels of different genes. Next, mass spectrometry, co-immunoprecipitation and immunofluorescence staining were used to detect the interactions of proteins. Furthermore, MTT assay, colony formation assay, wound-healing...

Registration Year

  • 2023
  • 2022
  • 2021

Resource Types

  • Collection


  • Rutgers, The State University of New Jersey
  • Shanghai Jiao Tong University
  • Zhejiang University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Chinese Academy of Sciences
  • West China Hospital of Sichuan University
  • Fudan University
  • Capital Medical University
  • Wuhan University
  • Chinese Center For Disease Control and Prevention