620,365 Works

Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China

Wen-Ping Guo, Bing Zhang, Yi-Han Wang, Gang Xu, Xiaoquan Wang, Xuebing Ni & En-Min Zhou
Abstract Background Four species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China. Methods Hard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to...

Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China

Wen-Ping Guo, Bing Zhang, Yi-Han Wang, Gang Xu, Xiaoquan Wang, Xuebing Ni & En-Min Zhou
Abstract Background Four species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China. Methods Hard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to...

Additional file 2: of Development of a transcriptomic database for 14 species of scleractinian corals

Yanjie Zhang, Qian Chen, James Xie, Yip Yeung, Baohua Xiao, Baoling Liao, Jianliang Xu & Jian-Wen Qiu
Alignment. The alignment of one-to-one homologous genes of 18 stony coral species. (FASTA 3031 kb)

Implications of tag positioning and performance on the analysis of cetacean movement

Evert Mul, Marie-Anne Blanchet, Martin Biuw & Audun Rikardsen
Abstract Background Satellite-linked animal-borne tags enable the study of free-ranging marine mammals. These tags can only transmit data while their antenna is above the surface for a sufficient amount of time. Thus, the position of the tag on the animal’s body will likely influence the quality and the quantity of location estimates. We explored the effects of tag placement and tag performance on the analysis of cetacean movement, by deploying two identical Argos tags 33...

Additional file 1: of Development of a transcriptomic database for 14 species of scleractinian corals

Yanjie Zhang, Qian Chen, James Xie, Yip Yeung, Baohua Xiao, Baoling Liao, Jianliang Xu & Jian-Wen Qiu
Table S1. Information on published transcriptome datasets from Scleractinia. Table S2. Symbiotic algae types determined by BLAST coral transcriptomes against the ITS2 and rRNA genes (i.e. 18S, 28S 23S rRNA) from GenBank database. Table S3. The accession numbers of sequences in the GenBank database used for symbiotic algae clade identification. (XLSX 179 kb)

Additional file 2: of Development of a transcriptomic database for 14 species of scleractinian corals

Yanjie Zhang, Qian Chen, James Xie, Yip Yeung, Baohua Xiao, Baoling Liao, Jianliang Xu & Jian-Wen Qiu
Alignment. The alignment of one-to-one homologous genes of 18 stony coral species. (FASTA 3031 kb)

Additional file 1: of Development of a transcriptomic database for 14 species of scleractinian corals

Yanjie Zhang, Qian Chen, James Xie, Yip Yeung, Baohua Xiao, Baoling Liao, Jianliang Xu & Jian-Wen Qiu
Table S1. Information on published transcriptome datasets from Scleractinia. Table S2. Symbiotic algae types determined by BLAST coral transcriptomes against the ITS2 and rRNA genes (i.e. 18S, 28S 23S rRNA) from GenBank database. Table S3. The accession numbers of sequences in the GenBank database used for symbiotic algae clade identification. (XLSX 179 kb)

Implications of tag positioning and performance on the analysis of cetacean movement

Evert Mul, Marie-Anne Blanchet, Martin Biuw & Audun Rikardsen
Abstract Background Satellite-linked animal-borne tags enable the study of free-ranging marine mammals. These tags can only transmit data while their antenna is above the surface for a sufficient amount of time. Thus, the position of the tag on the animal’s body will likely influence the quality and the quantity of location estimates. We explored the effects of tag placement and tag performance on the analysis of cetacean movement, by deploying two identical Argos tags 33...

Does changing from a teacher-centered to a learner-centered context promote self-regulated learning: a qualitative study in a Japanese undergraduate setting

Yasushi Matsuyama, Motoyuki Nakaya, Hitoaki Okazaki, Adam Lebowitz, Jimmie Leppink & Cees Van Der Vleuten
Abstract Background Previous studies indicate that a teacher-centered context could hinder undergraduates from self-regulated learning (SRL), whereas a learner-centered context could promote SRL. However, SRL development between a teacher-centered and a learner-centered context has not directly compared in undergraduate settings. Also, it is still unclear how a contextual change toward learner-centered learning could influence SRL in students, who are strongly accustomed to teacher-centered learning. Methods We conducted three focus groups that examined 13 Japanese medical...

Does changing from a teacher-centered to a learner-centered context promote self-regulated learning: a qualitative study in a Japanese undergraduate setting

Yasushi Matsuyama, Motoyuki Nakaya, Hitoaki Okazaki, Adam Lebowitz, Jimmie Leppink & Cees Van Der Vleuten
Abstract Background Previous studies indicate that a teacher-centered context could hinder undergraduates from self-regulated learning (SRL), whereas a learner-centered context could promote SRL. However, SRL development between a teacher-centered and a learner-centered context has not directly compared in undergraduate settings. Also, it is still unclear how a contextual change toward learner-centered learning could influence SRL in students, who are strongly accustomed to teacher-centered learning. Methods We conducted three focus groups that examined 13 Japanese medical...

Additional file 8: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
CAZyme profiles for lupin-infecting and reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 32 kb)

Additional file 7: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Summary the relationship between repetitive sequences and PAV regions in reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 1065 kb)

Additional file 8: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
CAZyme profiles for lupin-infecting and reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 32 kb)

Additional file 3: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Comparison of the repeat contents of isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 13 kb)

Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model

Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Nicole Tegtmeyer, Manja Boehm, Steffen Backert, Stefan Bereswill & Markus Heimesaat
Abstract Background Campylobacter jejuni infections constitute serious threats to human health with increasing prevalences worldwide. Our knowledge regarding the molecular mechanisms underlying host–pathogen interactions is still limited. Our group has established a clinical C. jejuni infection model based on abiotic IL-10−/− mice mimicking key features of human campylobacteriosis. In order to further validate this model for unraveling pathogen-host interactions mounting in acute disease, we here surveyed the immunopathological features of the important C. jejuni virulence...

Additional file 5: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
List of predicted effector genes in lupin-infecting isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 43 kb)

Additional file 3: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Comparison of the repeat contents of isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 13 kb)

Additional file 7: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Summary the relationship between repetitive sequences and PAV regions in reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 1065 kb)

Additional file 4: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Information of genes associated with AT-rich regions identified in the lupin-infecting Botrytis cinerea isolate. (XLSX 11 kb)

Additional file 5: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
List of predicted effector genes in lupin-infecting isolates of Botrytis cinerea and Sclerotinia sclerotiorum. (XLSX 43 kb)

Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model

Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Nicole Tegtmeyer, Manja Boehm, Steffen Backert, Stefan Bereswill & Markus Heimesaat
Abstract Background Campylobacter jejuni infections constitute serious threats to human health with increasing prevalences worldwide. Our knowledge regarding the molecular mechanisms underlying host–pathogen interactions is still limited. Our group has established a clinical C. jejuni infection model based on abiotic IL-10−/− mice mimicking key features of human campylobacteriosis. In order to further validate this model for unraveling pathogen-host interactions mounting in acute disease, we here surveyed the immunopathological features of the important C. jejuni virulence...

Additional file 6: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Summary of PAV regions spanning 3 or more genes in reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum, relative to alternate isolates of the same species. (XLSX 72 kb)

Additional file 6: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Summary of PAV regions spanning 3 or more genes in reference isolates of Botrytis cinerea and Sclerotinia sclerotiorum, relative to alternate isolates of the same species. (XLSX 72 kb)

Additional file 4: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Information of genes associated with AT-rich regions identified in the lupin-infecting Botrytis cinerea isolate. (XLSX 11 kb)

Additional file 2: of Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics

Mahsa Mousavi-Derazmahalleh, Steven Chang, Geoff Thomas, Mark Derbyshire, Phillip Bayer, David Edwards, Matthew Nelson, William Erskine, Francisco Lopez-Ruiz, Jon Clements & James Hane
Information supporting effector prediction, orthology, functional annotations, mutations and PAV across all isolate gene sets. (XLSX 5734 kb)

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