Identifying lipidomic key species in MS-data, applied to hepatocyte lipid droplets from stressed mouse modelsJürgen Hartler, Harald C Köfeler, Martin Trötzmüller & Friedrich Spener
Lipid droplets are considered to be the hub for storage and metabolism of cellular lipids. In previous work we have phenotyped the lipidome of murine hepatocyte lipid droplets using liquid chromatography-mass spectrometry (UHPLC-MS) plus integrated MS/MS, followed by automatic analysis of the MS data. The organelles were isolated after intervention studies involving nutritional stress (extended feeding of a high fat diet or short term fasting), genetic stress due to knock-out of adipocyte triglyceride lipase, or...
R and R markdown source code, and model output files for the manuscript: Saravia, L.A. (2015). A new method to analyse species abundances in space using generalized dimensions. Methods Ecol. Evol., 6, 1298–1310Available as a pre-print: https://dx.doi.org/10.7287/peerj.preprints.745v5 Other code used in the manuscript is - The neutral/hierarchical model: https://github.com/lsaravia/neutral - The generalized dimension estimation software: https://github.com/lsaravia/mfsba