630,567 Works

Development of an in vitro media perfusion model of Leishmania major macrophage infection

Alec O'Keeffe, Lauren Hyndman, Sean McGinty, Alaa Riezk, Sudax Murdan & Simon L Croft
Influence of the rate of flow (of culture medium) on infection of macrophage cells by L. major promastigotes

Phagocytosis of fluorescent latex beads and macropinocytosis of pHrodo Red dextran by infected macrophages (PEMs) under static or flow culture conditions

Data associated with the paper Antczak et al. "Environmental conditions shape the nature of a minimal bacterial genome" published in Nature Communications 2019

Magdalena Antczak, Martin Michaelis & Mark N Wass
Of the 473 genes in the genome of the bacterium with the smallest genome generated to date, 149 genes have unknown function, emphasising a universal problem; less than 1% of proteins have experimentally determined annotations. Here, we combine the results from state-of-the-art in silico methods for functional annotation and assigned functions to 66 of the 149 proteins. Proteins that are still not be annotated lack orthologs, lack protein domains, and/ or are membrane proteins. Twenty-four...

EVER-EST4GSNL@EGU2018

Dario Stelitano
Poster presented at EGU 2018, entitled: The 03-09 December 2015 Etna eruption volcanic parameters retrieved using Volcanic Plume Removal procedure on EVER-EST project platform, by Dario Stelitano, Ciro Manzo, Lorenzo Guerrieri, Stefano Corradini, Luca Merucci, Vito Romaniello, Elisa Trasatti, Stefano Salvi, and Giuliana Rubbia

Completeness of the known graptoloid palaeontological record

Michael Foote, Peter M. Sadler, Roger A. Cooper & James S. Crampton
Using a high-resolution chronology of graptoloid first and last appearances, we apply mathematical models that allow the simultaneous inference of the probability distribution of species durations and the effective sampling rate relating those durations to observed stratigraphic ranges. This approach allows the completeness of the documented palaeontological record of graptoloids to be assessed. We estimate that about 75% of species in the geographic regions that contribute to the stratigraphic data have been sampled, and that,...

Completeness of the known graptoloid palaeontological record

Michael Foote, Peter M. Sadler, Roger A. Cooper & James S. Crampton
Using a high-resolution chronology of graptoloid first and last appearances, we apply mathematical models that allow the simultaneous inference of the probability distribution of species durations and the effective sampling rate relating those durations to observed stratigraphic ranges. This approach allows the completeness of the documented palaeontological record of graptoloids to be assessed. We estimate that about 75% of species in the geographic regions that contribute to the stratigraphic data have been sampled, and that,...

Calotropis gigantea extract induces apoptosis through extrinsic/intrinsic pathways and reactive oxygen species generation in A549 and NCI-H1299 non-small cell lung cancer cells

Jiyon Lee, Hui-Ju Jang, Hyunwoo Chun, Thu-Huyen Pham, Yesol Bak, Jong-Woon Shin, Hang Jin, Yong-In Kim, Hyung Ryu, Sei Oh & Do-Young Yoon
Abstract Background Calotropis gigantea (CG) is a tall and waxy flower that is used as a traditional remedy for fever, indigestion, rheumatism, leprosy, and leukoderma. However, the precise mechanisms of its anticancer effects have not yet been examined in human non-small cell lung cancer (NSCLC) cells. In this study, we investigated whether CG extract exerted an apoptotic effect in A549 and NCI-H1299 NSCLC cells. Methods The ethanol extract of CG was prepared, and its apoptotic...

Calotropis gigantea extract induces apoptosis through extrinsic/intrinsic pathways and reactive oxygen species generation in A549 and NCI-H1299 non-small cell lung cancer cells

Jiyon Lee, Hui-Ju Jang, Hyunwoo Chun, Thu-Huyen Pham, Yesol Bak, Jong-Woon Shin, Hang Jin, Yong-In Kim, Hyung Ryu, Sei Oh & Do-Young Yoon
Abstract Background Calotropis gigantea (CG) is a tall and waxy flower that is used as a traditional remedy for fever, indigestion, rheumatism, leprosy, and leukoderma. However, the precise mechanisms of its anticancer effects have not yet been examined in human non-small cell lung cancer (NSCLC) cells. In this study, we investigated whether CG extract exerted an apoptotic effect in A549 and NCI-H1299 NSCLC cells. Methods The ethanol extract of CG was prepared, and its apoptotic...

High expression of metabolic enzyme PFKFB4 is associated with poor prognosis of operable breast cancer

Ling Yao, Lei Wang, Zhi-Gang Cao, Xin Hu & Zhi-Ming Shao
Abstract Background Enhanced glycolysis in tumors, known as the Warburg effect, provides the metabolic basis of enhanced cancer cell proliferation and metastasis. The Warburg pathway enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is a newly identified key kinase that regulates transcriptional reprogramming and cell proliferation. Here we show the prognostic value of PFKFB4 expression in patients with operable breast cancer. Methods PFKFB4 expression was evaluated by immunohistochemistry in surgical specimens retrospectively collected from 200 patients with histologically proven...

Heritability estimates of the position and number of facial hair whorls in Thoroughbred horses

Tamu Yokomori, Teruaki Tozaki, Hiroshi Mita, Takeshi Miyake, Hironaga Kakoi, Yuki Kobayashi, Kanichi Kusano & Takuya Itou
Abstract Objective According to oral traditions of horse caretakers and trainers, the differences in the position and number of facial hair whorls may be associated with temperamental traits. Elucidating genetic background of facial hair whorls and its relationship to temperamental traits may promote more efficient breeding and maintenance of racehorses. In this study, we estimated heritabilities of the position and number of facial hair whorls in Japanese Thoroughbred horses. Results The number of facial hair...

Heritability estimates of the position and number of facial hair whorls in Thoroughbred horses

Tamu Yokomori, Teruaki Tozaki, Hiroshi Mita, Takeshi Miyake, Hironaga Kakoi, Yuki Kobayashi, Kanichi Kusano & Takuya Itou
Abstract Objective According to oral traditions of horse caretakers and trainers, the differences in the position and number of facial hair whorls may be associated with temperamental traits. Elucidating genetic background of facial hair whorls and its relationship to temperamental traits may promote more efficient breeding and maintenance of racehorses. In this study, we estimated heritabilities of the position and number of facial hair whorls in Japanese Thoroughbred horses. Results The number of facial hair...

High expression of metabolic enzyme PFKFB4 is associated with poor prognosis of operable breast cancer

Ling Yao, Lei Wang, Zhi-Gang Cao, Xin Hu & Zhi-Ming Shao
Abstract Background Enhanced glycolysis in tumors, known as the Warburg effect, provides the metabolic basis of enhanced cancer cell proliferation and metastasis. The Warburg pathway enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4) is a newly identified key kinase that regulates transcriptional reprogramming and cell proliferation. Here we show the prognostic value of PFKFB4 expression in patients with operable breast cancer. Methods PFKFB4 expression was evaluated by immunohistochemistry in surgical specimens retrospectively collected from 200 patients with histologically proven...

Light pollution affects space use and interaction of two small mammal species irrespective of personality

Julia Hoffmann, Annika Schirmer & Jana Eccard
Abstract Background Artificial light at night (ALAN) is one form of human-induced rapid environmental changes (HIREC) and is strongly interfering with natural darkâ light cycles. Some personality types within a species might be better suited to cope with environmental change and therefore might be selected upon under ongoing urbanization. Results We used LED street lamps in a large outdoor enclosure to experimentally investigate the effects of ALAN on activity patterns, movement and interaction of individuals...

Primary hyperoxaluria diagnosed after kidney transplantation failure: lesson from 3 case reports and literature review

Ruiming Cai, Minzhuang Lin, Zhiyong Chen, Yongtong Lai, Xianen Huang, Guozhi Zhao, Xuekun Guo, Zhongtang Xiong, Juan Chen, Hui Chen, Qingping Jiang, Shaoyan Liu, Yuexin Yang, Weixiang Liang, Minhui Zou, Tao Liu, Wenfang Chen, Hongzhou Liu & Juan Peng
Abstract Background Primary hyperoxaluria (PH) is a rare inborn disorder of the metabolism of glyoxylate, which causes the hallmark production oxalate and forms insoluble calcium oxalate crystals that accumulate in the kidney and other organs. Since the manifestation of PH varies from recurrent nephrolithiasis, nephrocalcinosis, and end-stage renal disease with age at onset of symptoms ranging from infancy to the sixth decade, the disease remains undiagnosed until after kidney transplantation in some cases. Case presentation...

Light pollution affects space use and interaction of two small mammal species irrespective of personality

Julia Hoffmann, Annika Schirmer & Jana Eccard
Abstract Background Artificial light at night (ALAN) is one form of human-induced rapid environmental changes (HIREC) and is strongly interfering with natural darkâ light cycles. Some personality types within a species might be better suited to cope with environmental change and therefore might be selected upon under ongoing urbanization. Results We used LED street lamps in a large outdoor enclosure to experimentally investigate the effects of ALAN on activity patterns, movement and interaction of individuals...

Primary hyperoxaluria diagnosed after kidney transplantation failure: lesson from 3 case reports and literature review

Ruiming Cai, Minzhuang Lin, Zhiyong Chen, Yongtong Lai, Xianen Huang, Guozhi Zhao, Xuekun Guo, Zhongtang Xiong, Juan Chen, Hui Chen, Qingping Jiang, Shaoyan Liu, Yuexin Yang, Weixiang Liang, Minhui Zou, Tao Liu, Wenfang Chen, Hongzhou Liu & Juan Peng
Abstract Background Primary hyperoxaluria (PH) is a rare inborn disorder of the metabolism of glyoxylate, which causes the hallmark production oxalate and forms insoluble calcium oxalate crystals that accumulate in the kidney and other organs. Since the manifestation of PH varies from recurrent nephrolithiasis, nephrocalcinosis, and end-stage renal disease with age at onset of symptoms ranging from infancy to the sixth decade, the disease remains undiagnosed until after kidney transplantation in some cases. Case presentation...

Identification of a new vector species of avian haemoproteids, with a description of methodology for the determination of natural vectors of haemosporidian parasites

Rasa Bernotienė, Rita Žiegytė, Gabrielė Vaitkutė & Gediminas Valkiūnas
Abstract Background Haemosporidian parasites are transmitted by dipteran blood-sucking insects but certain vectors remain unidentified for the great majority of described species. Sensitive PCR-based methods are often used for the detection of haemosporidian infection in wild-caught insects. However, this approach alone cannot distinguish between different sporogonic stages and thus is insufficient to demonstrate that the parasites produce the infective stage (sporozoite), which is essential for transmission. To prove that PCR-positive insects could act as vectors,...

Identification of a new vector species of avian haemoproteids, with a description of methodology for the determination of natural vectors of haemosporidian parasites

Rasa Bernotienė, Rita Žiegytė, Gabrielė Vaitkutė & Gediminas Valkiūnas
Abstract Background Haemosporidian parasites are transmitted by dipteran blood-sucking insects but certain vectors remain unidentified for the great majority of described species. Sensitive PCR-based methods are often used for the detection of haemosporidian infection in wild-caught insects. However, this approach alone cannot distinguish between different sporogonic stages and thus is insufficient to demonstrate that the parasites produce the infective stage (sporozoite), which is essential for transmission. To prove that PCR-positive insects could act as vectors,...

Engineered mosaic protein polymers; a simple route to multifunctional biomaterials

Daniel Peters, Helen Waller, Mark Birch & Jeremy Lakey
Abstract Background Engineered living materials (ELMs) are an exciting new frontier, where living organisms create highly functional materials. In particular, protein ELMs have the advantage that their properties can be manipulated via simple molecular biology. Caf1 is a protein ELM that is especially attractive as a biomaterial on account of its unique combination of properties: bacterial cells export it as a massive, modular, non-covalent polymer which is resistant to thermal and chemical degradation and free...

Engineered mosaic protein polymers; a simple route to multifunctional biomaterials

Daniel Peters, Helen Waller, Mark Birch & Jeremy Lakey
Abstract Background Engineered living materials (ELMs) are an exciting new frontier, where living organisms create highly functional materials. In particular, protein ELMs have the advantage that their properties can be manipulated via simple molecular biology. Caf1 is a protein ELM that is especially attractive as a biomaterial on account of its unique combination of properties: bacterial cells export it as a massive, modular, non-covalent polymer which is resistant to thermal and chemical degradation and free...

Ear biometrics for patient identification in global health: a field study to test the effectiveness of an image stabilization device in improving identification accuracy

Lauren Etter, Elizabeth Ragan, Rachael Campion, David Martinez & Christopher Gill
Abstract Background In many low and middle-income countries (LMICs), difficulties in patient identification are a major obstacle to the delivery of longitudinal care. In absence of unique identifiers, biometrics have emerged as an attractive solution to the identification problem. We developed an mHealth App for subject identification using pattern recognition around ear morphology (Project SEARCH (Scanning EARS for Child Health). Early field work with the SEARCH App revealed that image stabilization would be required for...

Stapled EGFR peptide reduces inflammatory breast cancer and inhibits additional HER-driven models of cancer

Sabrina Maisel, Derrick Broka, Benjamin Atwell, Thomas Bunch, Robert Kupp, Shiv Singh, Shwetal Mehta & Joyce Schroeder
Abstract Background The human epidermal growth factor receptor (HER) family of transmembrane tyrosine kinases is overexpressed and correlates with poor prognosis and decreased survival in many cancers. The receptor family has been therapeutically targeted, yet tyrosine kinase inhibitors (TKIs) do not inhibit kinase-independent functions and antibody-based targeting does not affect internalized receptors. We have previously demonstrated that a peptide mimicking the internal juxtamembrane domain of HER1 (EGFR; EJ1) promotes the formation of non-functional HER dimers...

Stapled EGFR peptide reduces inflammatory breast cancer and inhibits additional HER-driven models of cancer

Sabrina Maisel, Derrick Broka, Benjamin Atwell, Thomas Bunch, Robert Kupp, Shiv Singh, Shwetal Mehta & Joyce Schroeder
Abstract Background The human epidermal growth factor receptor (HER) family of transmembrane tyrosine kinases is overexpressed and correlates with poor prognosis and decreased survival in many cancers. The receptor family has been therapeutically targeted, yet tyrosine kinase inhibitors (TKIs) do not inhibit kinase-independent functions and antibody-based targeting does not affect internalized receptors. We have previously demonstrated that a peptide mimicking the internal juxtamembrane domain of HER1 (EGFR; EJ1) promotes the formation of non-functional HER dimers...

Long-term slow slip event detected beneath the Shima Peninsula, central Japan, from GNSS data

Akio Kobayashi & Takahiro Tsuyuki
Abstract Long-term slow slip events (SSEs), the largest events among slow earthquakes, occur repeatedly along the Nankai Trough, southwest Japan. Their locations, near the locked zones of the plate interface responsible for great megathrust earthquakes in the Nankai Trough, suggest that these events influence conditions in this critical seismogenic region. Characterizing the spatiotemporal changes of long-term SSEs is important for understanding changes in the locked portions of the plate interface before major earthquakes. Two decades...

Ear biometrics for patient identification in global health: a field study to test the effectiveness of an image stabilization device in improving identification accuracy

Lauren Etter, Elizabeth Ragan, Rachael Campion, David Martinez & Christopher Gill
Abstract Background In many low and middle-income countries (LMICs), difficulties in patient identification are a major obstacle to the delivery of longitudinal care. In absence of unique identifiers, biometrics have emerged as an attractive solution to the identification problem. We developed an mHealth App for subject identification using pattern recognition around ear morphology (Project SEARCH (Scanning EARS for Child Health). Early field work with the SEARCH App revealed that image stabilization would be required for...

Expression analysis of the osteoarthritis genetic susceptibility mapping to the matrix Gla protein gene MGP

Colin Shepherd, Abigail Reese, Louise Reynard & John Loughlin
Abstract Background Osteoarthritis (OA) is a common disease of older individuals that impacts detrimentally on the quality and the length of life. It is characterised by the painful loss of articular cartilage and is polygenic and multifactorial. Genome-wide association scans have highlighted over 90 osteoarthritis genetic signals, some of which reside within or close to highly plausible candidate genes. An example is an association to polymorphisms within and adjacent to the matrix Gla protein gene...

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