Lay abstract
Exosomes are small vesicles that are released from biological cells. Exosomes are viewed as being tools for intracellular communication, and there is evidence that cancer cells can release exosomes to, for example, enable metastasis. Exosomes may be very useful for diagnostics, as the detection and characterization of exosomes may allow disease detection at early stages. However, exosomes must be isolated from blood or other biomaterials prior to analysis. This can be a challenging task,...

Supplementary ZIP file – LabVIEW script and associated files for control of Gilson 223 Sample Changer as a dip coater

To analyze DNA molecules, it is important that little contaminating DNA is present so that it does not drown out the signal of the DNA you are interested in. This paper describes a simple method of filtering out small DNA molecules so that only the large, interesting molecules are left behind. We do so by using filters that have holes of a very precisely defined size so that only DNA smaller than these holes can...

This collection contains all supplemental digital material for the text book:
Sumer, B.M. and Fuhrman, D.R. (2020) Turbulence in Coastal and Civil Engineering. World Scientific. DOI: 10.1142/10829
This includes data sets and the turbulence model intended for use in the hands-on exercises in Chapter 10.
This includes Matlab analysis examples from Chapter 4.
This also includes a Correnda document, listing known typographical errors.

The Alaiz experiment (ALEX17) was performed at Alaiz, located in Navarre region (Spain), a site consisting of a mountain range of 1000m above sea level (a.s.l.). To the North, along the prevailing wind direction, measurement equipment are located at a valley 500m lower in altitude bounded by a 750 m asl. hill located 6 km north of Alaiz.
The experimental layout aims to provide a high-quality database to explore wind flow phenomena and derive validation datasets...

The Alaiz experiment (ALEX17) was performed at Alaiz, located in Navarre region (Spain), a site consisting of a mountain range of 1000m above sea level (a.s.l.). To the North, along the prevailing wind direction, measurement equipment are located at a valley 500m lower in altitude bounded by a 750 m asl. hill located 6 km north of Alaiz.
The experimental layout aims to provide a high-quality database to explore wind flow phenomena and derive validation datasets...

This data collection contains designs for three dual-Doppler measurement campaigns in complex terrain.The designs are outputs of the Campaign Planning Tool (CPT). CPT was made to simplify the measurement campaign design process for multi-lidar systems.
The data collection contains three datasets, each corresponding to one individual complex terrain site.These three sites are denoted as:(1) Scottish site(2) Italian site(3) Turkish site

This data collection contains designs for three dual-Doppler measurement campaigns in complex terrain.The designs are outputs of the Campaign Planning Tool (CPT). CPT was made to simplify the measurement campaign design process for multi-lidar systems.
The data collection contains three datasets, each corresponding to one individual complex terrain site.These three sites are denoted as:(1) Scottish site(2) Italian site(3) Turkish site

Supplementary figures for: Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions

Automated cell counters that utilize still images of sample cells are widely used. However, they are not well suited to counting slender, aggregate-prone microorganisms such as Trypanosoma cruzi. Here, we developed a motion-based cell-counting system, using an image-recognition method based on a cubic higher-order local auto-correlation feature. The software successfully estimated the cell density of dispersed, aggregated, as well as fluorescent parasites by motion pattern recognition. Loss of parasites activeness due to drug treatment could...

Supplementary Table 1. Cell input modifications for GMD capture. Bacterial and algal concentrations were adjusted to cell concentrations OD600, OD750 in 1x PBS and 100 µl of the resultant cell suspension (or 50 + 50 µL for abGMDs) was used as cell input for initial capture in the CelGel Matrix prior to sorting. This ratio of bacteria-to-matrix or algae-to-matrix (# cells/GMD) was verified by microscopy; data not presented. “aGMD” = algae only GMDs, “bGMD” =...

Supplementary Table 1. Cell input modifications for GMD capture. Bacterial and algal concentrations were adjusted to cell concentrations OD600, OD750 in 1x PBS and 100 µl of the resultant cell suspension (or 50 + 50 µL for abGMDs) was used as cell input for initial capture in the CelGel Matrix prior to sorting. This ratio of bacteria-to-matrix or algae-to-matrix (# cells/GMD) was verified by microscopy; data not presented. “aGMD” = algae only GMDs, “bGMD” =...

Supplementary figure 1. Comparison of the new mismatch-tolerant RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5) DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants. The amplified products were detected using 2.5% agarose gel electrophoresis. The arrow highlights the specific product. For other details, please see Figure 3. Supplementary figure 2. Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences...

Supplementary figure 1. Comparison of the new mismatch-tolerant RT-qPCR with the assays with only Taq (conventional) and high fidelity (Q5) DNA polymerase. A. Detection of RSV mutants; B. Detection HCoV 229E mutants. The amplified products were detected using 2.5% agarose gel electrophoresis. The arrow highlights the specific product. For other details, please see Figure 3. Supplementary figure 2. Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences...

Supplementary Figure 1. Viability is unaffected by short time incubation in high viscosity medium. (A) The number of counted CFU/ml of Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the viability of Xanthomonas retroflexus was unaffected by incubation in high viscosity liquid and did not change over a short time period (N=3, Error bars represent Standard Error (SE)).

Supplementary Figure 1. MSC characterization prior to the genome editing. (A) The morphology of the MSCs. (B) The MSCs expressed MSC markers, analyzed by flow cytometry. (C) The MSCs could differentiate into adipocytes, osteocytes and chondrocytes. Supplementary Figure 2. Target inserting the IL-37 into AASV1 site. (A) Donor and gRNA structures. (B) PCR characterization of genome edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR product size. (C) PCR characterization of...

This collection contains all supplemental digital material for the text book:
Sumer, B.M. and Fuhrman, D.R. (2020) Turbulence in Coastal and Civil Engineering. World Scientific. DOI: 10.1142/10829
This includes data sets and the turbulence model intended for use in the hands-on exercises in Chapter 10.
This also includes Matlab analysis examples from Chapter 4.

This data collection contains designs for three dual-Doppler measurement campaigns in complex terrain.The designs are outputs of the Campaign Planning Tool (CPT). CPT was made to simplify the measurement campaign design process for multi-lidar systems.
The data collection contains three datasets, each corresponding to one individual complex terrain site.These three sites are denoted as:(1) Scottish site(2) Italian site(3) Turkish site

This data collection contains designs for three dual-Doppler measurement campaigns in complex terrain.The designs are outputs of the Campaign Planning Tool (CPT). CPT was made to simplify the measurement campaign design process for multi-lidar systems.
The data collection contains three datasets, each corresponding to one individual complex terrain site.These three sites are denoted as:(1) Scottish site(2) Italian site(3) Turkish site

Lay abstract Hairy roots have been used for various purposes over the last several years. In the present study we used Agrobacterium rhizogenes-mediated hairy roots as an alternative approach for Agrobacterium tumefaciens transformation. We developed a simple, effective and reproducible hairy root protocol in tobacco and Arabidopsis. Developed hairy roots were compared with intact roots to characterize the gene response to aluminum, NaCl stress and cellular localization of genes. Aluminum-responsive orthologous genes function could be...

Figure S1. The effect of excessive TMPD on cisplatin- induced DNA base damage.Figure S1 shows the percentage peak value of 500 M dGMP mixed with various concentrations of cisplatin only (left), and cisplatin combined with 1 mM TMPD (right) after 24 hours of reaction. The percentage peak value without cisplatin treatment was designated as 100%. Scan rate: 20 mV/s
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of...

Automated cell counters that utilize still images of sample cells are widely used. However, they are not well suited to counting slender, aggregate-prone microorganisms such as Trypanosoma cruzi. Here, we developed a motion-based cell-counting system, using an image-recognition method based on a cubic higher-order local auto-correlation feature. The software successfully estimated the cell density of dispersed, aggregated, as well as fluorescent parasites by motion pattern recognition. Loss of parasites activeness due to drug treatment could...