8,551 Works

Novel deletion alleles of a C. elegans gene Y73E7A.1, named as tm6429 and tm6475

Yuji Suehiro, Sawako Yoshina, Sayaka Hori & Shohei Mitani
We report tm6429 and tm6475 as novel deletion alleles of the gene Y73E7A.1 that is a homologue of mammalian Coiled-coil domain containing 124 (Ccdc124)1. The Ccdc124 is a conserved gene from invertebrates to human. In human cell lines, Ccdc124 is a component of the centrosome during interphase and at the G2/M transition. During cell division, Ccdc124 relocates to the midbody at telophase and acts as an essential molecular component in cytokinesis2. The alleles were isolated...

SPAS-1 expression in neurons and vulva during L4 stage

George Brown & Rachid El Bejjani
spas-1 is the worm homolog of the human spastic paraplegia gene, spastin. Previous work in C. elegans has shown that SPAS-1 disassembles microtubules in an ATP-dependent manner (Matsushita-Ishiodori et al, 2007). Another study shows that SPAS-1 plays a role in synapse remodeling in motor neurons (Kurup et al, 2015). Here we describe the expression of SPAS-1 in L4 hermaphrodite larvae using the transcriptional reporter, axrEx8 [Pspas-1:mCh]: A) Low magnification (10X objective) image of an entire...

oxi-1 and fshr-1 are required for neuromuscular signaling under normal and oxidative stress conditions in C. elegans

Barry Wei & Jennifer R Kowalski
Reactive oxygen species (ROS) contribute to neuronal degeneration by readily reacting with cellular components, consequently breaking down cellular integrity. Excess ROS often leads to oxidative stress, which results from destabilization of the organism's ability to control the balance between antioxidants and free radicals (Chandra et al. 2015). The ubiquitin-proteasome system helps to regulate oxidative stress and overall damage to cellular components by forming chains of ubiquitin polypeptides on cellular proteins; these chains then serve as...

Direct N-body code designed for a cluster based on heterogeneous computational nodes

David Goz, Luca Tornatore, Sara Bertocco & Giuliano Taffoni
The ExaNeSt H2020 project is developing an exascale ready prototype based on hybrid hardware (CPUs+accelerators). Astrophysical codes are used to test and validate the exascale platform. At the same time, astrophysical applications must be re-engineered in order to exploit heterogeneous hardware. We present and test the parallel scheme of a state-of-the-art direct N -body code (called ExaHiGPUs) conceived to be run on hybrid architecture.

The bodies of dpy-10(e128) are twice as stiff as wild type

Sylvia Fechner, Frédéric Loizeau, Adam L Nekimken, Beth L Pruitt & Miriam B Goodman
DPY-10 is a collagen protein in the nematode's cuticle. Mutations in the dpy-10 gene induce various morphological changes that lead to animals with a dumpy or roller phenotype (Levy, Yang & Kramer, 1993). Here, we asked how such mutations affect body stiffness by comparing force-indentation curves in dpy-10(e128) and wild type worms. On average, dpy-10(e128) worms have a steeper force-indentation relationship, leading to a higher body stiffness (Fig 1.C). Average stiffness values were 2.5 0.9...

Extending the CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences

Annabel Ebbing, Peng Shang, Niels Geijsen & Hendrik C. Korswagen
The CRISPR/Cas9 system has become a powerful tool for genome-editing in C. elegans. Sequence specificity of the system is determined by a guide RNA which targets the Cas9 endonuclease to the genomic region of interest. In addition, Cas9 needs to interact with a so-called protospacer-adjacent motif (PAM) next to the target sequence. In case of Cas9 from Streptococcus pyogenes (SpCas9), the most commonly used Cas9, this sequence is NGG. Because the C. elegans genome sequence...

Microfluidic EPG Recordings Show Striking Pharyngeal Pumping Phenotype in a C. elegans Alzheimer's Disease Model

Janis C Weeks, Kristen J Robinson, Benedicta Wanjeri, Philip F Copenhaver & William M Roberts
Strain CL4176 accumulates human amyloid-β1-42 in body wall muscles after animals are shifted from 15 to 25 oC, causing paralysis (Link et al. 2003). We temperature-shifted worms at early L4 and quantified pharyngeal pumping at 48 h using a microfluidic electropharyngeogram (EPG) recording platform (Lockery et al. 2012; Weeks et al. 2016). Recordings were made in M9 buffer with 10 mM serotonin and analyzed by automated pump-recognition software. At 48 h, control CL802 worms showed...

Two new functions in the WormBase Enrichment Suite

David Angeles-Albores, Raymond Y.N. Lee, Juancarlos Chan & Paul W. Sternberg
​Genome-wide experiments routinely generate large amounts of data that can be hard to interpret biologically. A common approach to interpreting these results is to employ enrichment analyses of controlled languages, known as ontologies, that describe various biological parameters such as gene molecular or biological function. In C. elegans, three distinct ontologies, the Gene Ontology (GO), Anatomy Ontology (AO), and the Worm Phenotype Ontology (WPO) are used to annotate gene function, expression and phenotype, respectively (Ashburner...

SapTrap vectors for introducing point mutations with unc-119+ selection

Matthew L Schwartz & Erik M Jorgensen
SapTrap assembly generates CRISPR targeting vectors using a Golden Gate cloning strategy (Schwartz and Jorgensen, 2016). The original toolkit was designed for inserting tags, such as fluorescent proteins or affinity tags, into protein coding genes in C. elegans. In the original design, the floxed unc-119+ selectable marker was incorporated into the tag. For site-directed mutagenesis, the selectable marker is useful but a tag is unnecessary. We have developed a new set of vectors dedicated for...

CATCH-EyoU: Processes in Youth’s Construction of Active EU Citizenship: Survey Data: Estonia: Wave 2

Peter Noack, Petr Macek, Veronika Kalmus & Andu Rämmer
The data set was generated within the research project Constructing AcTive CitizensHip with European Youth: Policies, Practices, Challenges and Solutions (CATCH-EyoU) funded by European Union, Horizon 2020 Programme - Grant Agreement No 649538. The data set is a truncated version of the adolescents’ and young adults’ survey that was carried out in Estonia from November 2017 to February 2018. It merges results of two polls (15-19 and 20-30 year olds). Survey was conducted by University...

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing

Leif Benner & Brian Oliver
A number of reagents have been generated for CRISPR/Cas9 genome editing in Drosophila (Gratz et al. 2014; Ren et al. 2013; Kondo and Ueda 2013; Port et al. 2014; Port and Bullock 2016). The ability to efficiently express multiple gRNAs from a single vector provides the advantage of creating multiple CRISPR/Cas9 alleles at a time. An elegant design has previously shown that gRNAs separated by tRNAs allow for genomic editing at multiple loci in Drosophila...

Riigikaitse ja avalik arvamus 2001-06

Juhan Kivirähk
Andmetele ligipääsu taotlemiseks palun võtta ühendust Eesti Sotsiaalteadusliku andmearhiiviga aadressil socarch@psych.ut.ee

Nuclear localization of a C. elegans CCCH-type zinc finger protein encoded by T26A8.4

Takashi Koyama & Chisato Ushida
T26A8.4 encodes a CCCH-type zinc finger protein. The amino acid sequence of this protein is partially similar to that of S. cerevisiae Caf120p, which has no zinc finger domain. T26A8.4-encoded RNA was detected more abundantly in germ cells than in other tissues of L4-adult worms according to the NEXTDB data. When expressed specifically in the germline under control of the mex-5 promoter, the T26A8.4-encoded protein localized to germ cell nuclei in the adult hermaphrodite and...

How Accountable Care Organizations Use Population Segmentation to Care for High-Need, High-Cost Patients

Ann S. O'Malley, Eugene C. Rich, Rumin Sarwar, Eli Schultz, W. Cannon Warren, Tanya Shah & Melinda K. Abrams

Lęk intymności, czyli mówienie prawdy w dobie internetu

Leszek Koczanowicz
Kultura Współczesna 101, 2 (2018), pp. 38-50

Why Easier Access to Hearing Aids Is Not Enough

Amber Willink, Nicholas S. Reed & Frank R. Lin

Expression pattern of endogenous PAR-4A & C after CRISPR/Cas9-mediated genome editing

Vincent Roy, Olivier Gagné, Karim Hamiche, Jean-Claude Labbé & Patrick Narbonne
PAR-4/LKB1 is maternally expressed and required for the asymmetrical distribution of early embryonic determinants and viability in C. elegans (Morton et al. 1992; Watts et al. 2000; Tenlen et al. 2008). It is also implicated in a variety of postembryonic processes, including germline stem cell quiescence (Narbonne and Roy 2006; Narbonne et al. 2017), neuronal growth and polarity (Kim et al. 2010; Teichmann and Shen 2011), cytoskeletal rearrangements (Narbonne et al. 2010; Chartier et al....

Simplified detection of a point mutation in C. elegans using tetra-primer ARMS-PCR

Matthew T Sullenberger & Eleanor M Maine
Single nucleotide polymorphisms (SNPs) can be difficult to detect using traditional PCR and gel electrophoresis, especially when no enzyme restriction sites overlap the SNP. Although alleles can be identified through amplification with flanking primers and sequencing, this adds time and cost, and sequencing is typically outsourced. Tetra-primer Amplification-Refractory Mutation System (ARMS)-PCR allows SNPs to be distinguished through a 3' primer mismatch at the SNP site for one allele, and an additional mismatch 1 - 3...

Diversity in symbiotic specificity of bacterial strains nodulating lupins in Poland

Krzysztof Pudełko & Joanna Żarnicka

Response of durum wheat (Triticum durum Desf.) to protection intensity

Grzegorz Szumiło & Leszek Rachoń

The evaluation of grain and flour quality of spring durum wheat (Triticum durum Desf.)

Grzegorz Szumiło , Leszek Rachoń & Sławomir Stankowski

Possibilities of weed control in spring cereals by herbicides applied at reduced rates

Krzysztof Domaradzki & Renata Kieloch

Agro-ecological assessment of the vegetation cover of Poland’s soils

Adam Harasim

An implementation of environmental databases for physical modeling of water balance and land degradation

Tomasz Stuczyński , Artur Łopatka , Bernadetta Zawadzka & Grzegorz Siebielec

What Is the Status of Women’s Health and Health Care in the U.S. Compared to Ten Other Countries?

Munira Z. Gunja, Roosa Tikkanen, Shanoor Seervai & Sara R. Collins
Women in the United States have long lagged behind their counterparts in other high-income countries in terms of access to health care and health status. This brief compares U.S. women’s health status, affordability of health plans, and ability to access and utilize care with women in 10 other high-income countries by using international data.

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