148 Works

The Mapping of Drosophila melanogaster mutant A.4.4

Kayla L Bieser, Joyce Stamm, Ayala A Aldo, Suneil Bhaskara, Makayla Claiborne, Joselyn N Coronel Gómez, Ron Dean, Aaron Dowell, Evan Dowell, Mathew Eissa, Ahmad A Fawaz, Michael M Fouad-Meshriky, Dustin Godoy, Krista Gonzalez, Malak K Hachem, Malak F Hammoud, Anthony Huffman, Hunter Ingram, Alex B Jackman, Bibek Karki, Natalia Khalil, Houda Khalil, Tran Khanh Ha, Arjun Kharel, Izabell Kobylarz … & Jacob D Kagey
A novel Drosophila melanogaster mutant A.4.4 was isolated from a conditional Flp/FRT mosaic eye screen in the context of blocked apoptosis (Kagey et al., 2012). The ;FRT42D, Dark82 chromosome was used as a starting point for the EMS mutagenesis screen to screen for mutations that conferred a growth advantage in the environment of blocked apoptosis via the homozygous Dark82 allele (Akdemir et al., 2006). Mutants were screened for over-representation of mutant tissue (pigmented) as compared...

Information and communication technology use in Australian agriculture

Niki Dufty & Thomas Jackson
New information and communications technology (ICT) could deliver the next wave of productivity growth in Australian agriculture. ICT includes all digital technologies that facilitate the electronic capture, processing, storage and exchange of information. To better understand the role of ICT in Australian agriculture and potential barriers to its use, ABARES surveyed over 2,200 farmers in 2016–17. Results from this survey are presented here for broadacre, dairy and vegetable farms.

Direct N-body code designed for a cluster based on heterogeneous computational nodes

David Goz, Luca Tornatore, Sara Bertocco & Giuliano Taffoni
The ExaNeSt H2020 project is developing an exascale ready prototype based on hybrid hardware (CPUs+accelerators). Astrophysical codes are used to test and validate the exascale platform. At the same time, astrophysical applications must be re-engineered in order to exploit heterogeneous hardware. We present and test the parallel scheme of a state-of-the-art direct N -body code (called ExaHiGPUs) conceived to be run on hybrid architecture.

Efficient FLP-mediated germ-line recombination in C. elegans

Javier Macías-León & Peter Askjaer
​Recombinases are very useful enzymes for spatiotemporal control of gene expression (Hubbard 2014). Through binding to specific DNA sequences, they can be used to excise or exchange DNA fragments. We have previously generated a series of Caenorhabditis elegans strains that stably express an efficient FLP recombinase in specific tissues and reported how they enable precise regulation of gene expression, cell ablation or conditional gene knockout (Muñoz-Jiménez et al 2017). However, these strains are restricted to...

After the ACA Simplified Medicaid Enrollment, the Administration and Some States Are Making It Far More Complicated

Sara Rosenbaum, Maria Velasquez, Sara Rothenberg & Rachel Gunsalus
For years, the federal government and states have sought to get rid of enrollment barriers in Medicaid. Now a number of states are reversing course: 14 states have approved or pending Medicaid 1115 demonstrations designed to restrict Medicaid eligibility. Attention has focused on the demonstrations’ work mandates, but the experiments involve much more. Other features include extensive reporting rules — in some proposals enrollees must report on their eligibility status weekly — as well as...

The Cost of Employer Insurance Is a Growing Burden for Middle-Income Families

Sara R. Collins & David C. Radley
Recent national surveys show health care costs are a top concern in U.S. households.1 While the Affordable Care Act’s marketplaces receive a lot of media and political attention, the truth is that far more Americans get their coverage through employers. In 2017, more than half (56%) of people under age 65 — about 152 million people — had insurance through an employer, either their own or a family member’s.2 In contrast, only 9 percent had...

SapTrap vectors for introducing point mutations with unc-119+ selection

Matthew L Schwartz & Erik M Jorgensen
SapTrap assembly generates CRISPR targeting vectors using a Golden Gate cloning strategy (Schwartz and Jorgensen, 2016). The original toolkit was designed for inserting tags, such as fluorescent proteins or affinity tags, into protein coding genes in C. elegans. In the original design, the floxed unc-119+ selectable marker was incorporated into the tag. For site-directed mutagenesis, the selectable marker is useful but a tag is unnecessary. We have developed a new set of vectors dedicated for...

GFPnovo2, a brighter GFP variant for in vivo labeling in C. elegans

Ardalan Hendi & Kota Mizumoto
Green fluorescent protein (GFP) is one of the most common fluorophores used to label cells and proteins in C. elegans. Previous artificial protein evolution strategy has generated a variant of eGFP, GFPnovo2, which is 3.3 times brighter than the original eGFP in the DT40 cell line (Arakawa et al, 2008). GFPnovo2 carries four mutations from the original eGFP (Y145F, V163A, S202T, L221V), and has the same excitation/emission wavelengths as the original eGFP. Here we compared...

Rapamycin-induced protein dimerization as a tool for C. elegans research

Sriyash Mangal, Jeffrey Zielich, Eric J. Lambie & Esther Zanin
Induced protein dimerization is an invaluable tool in cellular biology to study protein function. Induced dimerization has been widely used to modulate enzymatic activity as well as expression and localization of proteins (Voß et al., 2015; DeRose et al., 2013). A popular method employs the chemical dimerizer rapamycin to induce binding between the FRB domain of the mTOR kinase and the FKBP12 protein (FK506 binding protein 12 kDa) (Putyrski and Schultz, 2012). Rapamycin-induced protein dimerization...

Targeting Phage and Antibiotic Resistance 2018

International Society Of Microbiota
The 5th World Congress on Targeting Infectious Diseases: Targeting Phage & Antibiotic Resistance 2018 was organized in Florence, Italy in May 17-18, 2018. During two days, more than 100 oral & poster communications were highlighted different axes and topics related to the recent advances and challenges in phage therapy, and to all innovations related to the antibiotic resistance in general. The 5th edition was an excellent platform which gathered more than 200 participants from 33...

A snapshot of Australian Fisheries and Aquaculture

Australian Bureau Of Agricultural Resource Economics And Sciences (ABARES)

The Effect of an Environmentally Relevant Phthalate Mixture on Primordial Germ Cells of Xenopus laevis Embryos

Nathalie L Momplaisir, Aurora Turgeon, Jodi Flaws & Jing Yang
Phthalates, being considered endocrine disrupting chemicals, are estrogen-like and anti-androgenic compounds that interfere with hormones which regulate numerous biological processes, including the maintenance of homeostasis and the regulation of development (Kavlock et al., 1996). Toxicological studies have consistently shown that phthalate metabolites are reproductive and developmental toxicants (Jeng, 2014). Some of the significant complications include fertility issues, decreased anogenital distance, low sperm concentration and motility. While the adverse effects of phthalates on the reproductive system...

How ACOs Are Caring for People with Complex Needs

Kristen A. Peck, Benjamin Usadi, Alexander Mainor, Helen Newton & Ellen Meara
ABSTRACT Issue: With an incentive to provide high-quality care while controlling costs, accountable care organizations (ACOs) may focus on patients who require the most resources and are most at risk for encountering serious problems with their care. Understanding how ACOs approach care for complex patients requires examination of their organizational strategies, contracting details, and leadership structures. Goals: Describe the specific strategies employed by ACOs that have comprehensive care management programs and processes for complex patients....

The Electronic Health Record Problem

David Blumenthal
It’s no secret that many physicians are unhappy with their electronic health records (EHRs). They say they spend too much time keying in data and too little making eye contact with patients. They say their electronic records are clunky, poorly designed, hard to navigate, and cluttered with useless detail that colleagues have cut and pasted to meet documentation requirements. Meanwhile, the data they really need are buried almost beyond retrieval.

GFP::PCN-1 does not reliably mark S phase in C. elegans adult germline progenitor zone cells

Tokiko Furuta & Swathi Arur
PCNA (proliferating cell nuclear antigen) is the DNA polymerase processivity factor that loads onto the chromatin during S phase of the cell-cycle (Brauchle et al. 2003). Thus, nuclear localization of PCNA (PCN-1 in C. elegans) is used as a marker for the S phase of the cell cycle (Brauchle et al. 2003). GFP::PCN-1 has been shown to label S phase in C. elegans embryo when driven through the germline and embryonic promoter pie-1 (Brauchle et...

Le cancer au Canada : stade au moment du diagnostic

Shirley Bryan, Huda Masoud, Ryan Woods, Gina Lockwood, Hannah K. Weir, Nadine Badets, Leah Smith, James Brierley & Mary Gospodarowicz
Cette étude établit l'incidence du cancer par stade au moment du diagnostic pour les quatre principaux cancers, au moyen de données tirées du Registre canadien du cancer pour la période de 2011 à 2015.

Биомониторинговые возможности микроорганизмов при оценке степени токсичности синтетических поверхностно-активных веществ

Л.В. Кондакова, Л.И. Домрачева, Т.Я. Ашихмина & В.С. Симакова
Доказана возможность использования отдельных групп микроорганизмов для выявления степени токсичности синтетических поверхностно-активных веществ (СПАВ), которые используются в качестве автошампуней. Показана высокая чувствительность двух видов цианобактерий Nostoc paludosum и Fischerella muscicola, применяемых в качестве тест-организмов на действие трёх марок автошампуней. При инкубировании культур цианобактерий в растворах автошампуней с концентрацией, равной рекомендуемой дозе (1 р. д.), происходит резкое падение численности жизнеспособных клеток. Биотестирование с помощью бацилл проводили путём высева бактериальных суспензий на питательный агар с предварительно внесённым...

Snapshot of Australia's agricultural workforce

Bill Binks, Nyree Stenekes, Heleen Kruger & Robert Kancans
his snapshot describes Australia’s agricultural workforce, providing key information and statistics in one place. We cover where workers live, what sub-industries and occupations they work in, and the mobility and educational attainment of the workforce. The snapshot also describes the involvement of Indigenous people, People from Culturally and Linguistically Diverse backgrounds, women and young people in the agricultural workforce.

Silencing the ASI gustatory neuron pair increases expression of the stress-resistance gene sod-3 in a daf-16 and daf-3 independent manner

Peter Chisnell & Cynthia Kenyon
Ablation of the gustatory neuron pair ASI has been shown to extend lifespan, and this lifespan extension requires the function of the transcription factor daf-16/FOXO (Alcedo and Kenyon 2004). Given this relationship, we tested whether silencing the neuron pair ASI with the tetanus toxin light chain (Tetx), as opposed to ablating it, could increase the expression of a well- known DAF-16 target, the stress-resistance gene sod-3 (Furuyama et al. 2000; Honda and Honda 1999). Tetanus...

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  • 2018
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