Designing and building DNA reagents for CRISPR edits can be complicated as well as tedious. To streamline reagent design for our SapTrap CRISPR plasmid assembly system, we developed the SapTrap Builder software utility. SapTrap Builder provides an intuitive graphical user interface for visualizing the design of reagents for CRISPR-based genetic tag insertion and point mutagenesis. Our program guides users through several steps of CRISPR edit design, alerts the user when incompatibilities arise, and generates sequences...
Proprioseiopsis mauiensis (Prasad, 1968) and P. sharovi (Wainstein, 1975) are new species records for German mite fauna. They are re-described and illustrated.
Monthly variation of leaf litter Collembola in the tropical rainforest of Los Tuxtlas, Veracruz, MexicoJosé G. Palacios, Blanca E. Mejía, Rosamond Coates, Martha Madora & Gabriela Castaño
In order to evaluate leaf litter Collembola at the tropical rainforest of Los Tuxtlas, Veracruz, México, fifteen pitfall traps were activated monthly for about one week, in a new experimental area during 2015. A total of 4,291 specimens were captured in the 180 samples collected. Ten families were recorded, those better represented were Paronellidae (27 %), Neanuridae (20.5 %), Entomobryidae (16.9 %), Dicyrtomidae (15 %), and Isotomidae (11.6 %). Among the 32 genera reported, most abundant were Trogolaphysa (18 %), Ptenothrix...
Correct sampling and preparation of living material build up the heart of all natural history collections. Ideally, the samples outlive their collectors and preparators for centuries. This article gives advice on correct and durable storage of either mounted or ethanol stored material as well as on correct sampling during field work. Furthermore, the advantages and disadvantages of a number of the most common ant preparation techniques for inner and outer body characters are presented, compared,...
We have generated a novel null allele, ot900, of the C. elegans LIM homeodomain gene ceh-14. All existing deletion alleles of ceh-14 were generated using a Tc1 transposon insertion, one of which, ch3, is a putative null based on the resulting frame-shift and a reduction in detectable mRNA and protein levels (Cassata et al. 2000). However, in our hands we have noticed a propensity of the ch3 allele to revert to wild-type after several generations,...
On the life cycle and parasitism of the trombiculid mite Hirsutiella hexasternalis (Kudryashova, 1998) (Acariformes, Trombiculidae)Andrey B. Shatrov
Post-larval life cycle, larval morphology and histology of the sites of the larvae feeding were investigated in the trombiculid mite Hirsutiella hexasternalis (Kudryashova, 1998) based on the material firstly collected from West Siberia (Tyumen province) from the voles Myodes rutilus (Pallas, 1779). Duration of the nymphal developmental stages generally corresponds to those of other trombiculid mites with the average values of 19 and 13 days for proto- and tritonymphs, respectively. Duration of active deutonymphal stage...
sma-4 encodes the co-Smad of the BMP pathway in C. elegans, which is also known as the Sma/Mab pathway (Savage et al. 1996). Null mutations in core components of this pathway, including the BMP ligand DBL-1, the receptors SMA-6 and DAF-4, and the R-Smads SMA-2 and SMA-3, all result in a small body size phenotype without significantly compromising viability (Gumienny and Savage-Dunn 2013). However, we found that even after multiple rounds of out-crossing, two deletion...
Increasing awareness for soil biodiversity and protection The international touring exhibition “The Thin Skin of the Earth”Willi E. R. Xylander & Helga Zumkowski-Xylander
The Senckenberg Museum of Natural History Görlitz developed an international touring exhibition with the title “The thin skin of the earth – Our soils” as the national contribution to the International Year of the Soil 2015. Since the opening of the exhibition in October 2015 it had been shown at six localities and seen by more than 120.000 visitors. It will tour at least for another four years. The exhibition deals with soil biodiversity, the...
A number of reagents have been generated for CRISPR/Cas9 genome editing in Drosophila (Gratz et al. 2014; Ren et al. 2013; Kondo and Ueda 2013; Port et al. 2014; Port and Bullock 2016). The ability to efficiently express multiple gRNAs from a single vector provides the advantage of creating multiple CRISPR/Cas9 alleles at a time. An elegant design has previously shown that gRNAs separated by tRNAs allow for genomic editing at multiple loci in Drosophila...
The ExaNeSt H2020 project aims at the design and development of an exascale ready supercomputer with a low energy consumption profile but able to support the most demanding scientific and technical applications. The project will produce a prototype based on hybrid hardware (CPUs+accelerators) implementing a co-design approach where scientific applications requirements are driving the hardware design. Astrophysical codes are playing a fundamental role to validate the exascale platform. We present strategies adopted in order to...
Lazy hitchhikers? Preliminary evidence for within-habitat phoresy in pygmephoroid mites (Acari, Scutacaridae)Julia Baumann, Francisco Ferragut & Sanja Šimić
Mites of the family Scutacaridae have been found in termite nests and also phoretic on termites. In contrast to what has been reported until now for scutacarid mites, the respective species did not possess claws on leg I for attachment on their host, but clasped to the termites’ coxae probably by using their large, pad-like empodia on legs II and III. This mode of attachment is a totally new and unique discovery for Scutacaridae. The...
A Mechanical Model of Heart Valves with Chordae for in Silico Real-Time Computations and Cardiac Surgery PlanningGediminas GAIDULIS, Rimantas KAČIANAUSKAS, Natalya KIZILOVA & Yury ROMASHOV
Vol. 66, No. 4, pp. , 66(4): 471–492
The Italian Report to the 42nd COSPAR General Assembly to be held in Pasadena, edited by the National Institute of Astrophysics (INAF),the formal Italian national body that by the law supports the COSPAR activities, with the collaboration of ASI, the Italian Space Agency, and the other stakeholders playing a major role in the Italian scientific space programmes (INFN, CNR, INGV, etc.). This Report summarizes the last two years of space science activity in Italy.
Distribution and diversity of earthworms (Lumbricidae) in Hesse (Central Germany): current knowledgeJörg Römbke, Wolfgang H. O. Dorow & Stephan Jänsch
In 2016 the first Red List of German earthworms was compiled, listing 46 species. However, little is known about the biodiversity and distribution of Lumbricidae in the individual federal states of Germany. In some of them (e.g. Schleswig-Holstein) earthworm monitoring programs were performed (or are still running). In others no such soil-focused program has been performed so far. The aim of this contribution is to compile the available information on the distribution of earthworms (Lumbricidae)...
Silencing the ASI gustatory neuron pair increases expression of the stress-resistance gene sod-3 in a daf-16 and daf-3 independent mannerPeter Chisnell & Cynthia Kenyon
Ablation of the gustatory neuron pair ASI has been shown to extend lifespan, and this lifespan extension requires the function of the transcription factor daf-16/FOXO (Alcedo and Kenyon 2004). Given this relationship, we tested whether silencing the neuron pair ASI with the tetanus toxin light chain (Tetx), as opposed to ablating it, could increase the expression of a well- known DAF-16 target, the stress-resistance gene sod-3 (Furuyama et al. 2000; Honda and Honda 1999). Tetanus...
This brief is based on a presentation by ABARES’ Executive Director to the National Drought Summit in October 2018 describing the effects of the current drought on Australian agriculture. The brief summarises the extent of rainfall deficiencies and the effects on crop production, livestock markets and farm incomes.
Instytut Podstawowych Problemów Techniki PAN24
Journal «Theoretical and applied ecology»16
The Commonwealth Fund Website14
Société Française d'Ichtyologie13
Senckenberg Museum für Naturkunde Görlitz13
Department of Agriculture and Water Resources - ABARES11
Istituto Nazionale di Astrofisica8
Edith Cowan University1
Università degli Studi di Milano1
Plutof. Data Management and Publishing Platform1
Histarch - Historische Archäologie1