Correct sampling and preparation of living material build up the heart of all natural history collections. Ideally, the samples outlive their collectors and preparators for centuries. This article gives advice on correct and durable storage of either mounted or ethanol stored material as well as on correct sampling during field work. Furthermore, the advantages and disadvantages of a number of the most common ant preparation techniques for inner and outer body characters are presented, compared,...
PCNA (proliferating cell nuclear antigen) is the DNA polymerase processivity factor that loads onto the chromatin during S phase of the cell-cycle (Brauchle et al. 2003). Thus, nuclear localization of PCNA (PCN-1 in C. elegans) is used as a marker for the S phase of the cell cycle (Brauchle et al. 2003). GFP::PCN-1 has been shown to label S phase in C. elegans embryo when driven through the germline and embryonic promoter pie-1 (Brauchle et...
Increasing awareness for soil biodiversity and protection The international touring exhibition “The Thin Skin of the Earth”Willi E. R. Xylander & Helga Zumkowski-Xylander
The Senckenberg Museum of Natural History Görlitz developed an international touring exhibition with the title “The thin skin of the earth – Our soils” as the national contribution to the International Year of the Soil 2015. Since the opening of the exhibition in October 2015 it had been shown at six localities and seen by more than 120.000 visitors. It will tour at least for another four years. The exhibition deals with soil biodiversity, the...
The operating context of Australian agriculture has changed dramatically since Australian governments first committed to providing forecasting services for agriculture in the 1940s. This paper explores the evolution of these forecasting services, how the world has changed since then, and what this means for the future design of these services.
We modified Norris’s mCherry-tag repair donor vector by fusing a C. elegans codon-optimized fkbp12 sequence 3’ to mCherry (Mangal et al. 2018) and re-establishing the critical NotI site for 3’ homology region insertion (Figure 1 A; pEL226). This vector can be used to C-terminally tag any GOI by following the protocol of Norris et al. (2015), including subsequent Cre-mediated excision of the dual marker selection cassette (Figure 1 B). As proof of principle we C-terminally...
Monthly variation of leaf litter Collembola in the tropical rainforest of Los Tuxtlas, Veracruz, MexicoJosé G. Palacios, Blanca E. Mejía, Rosamond Coates, Martha Madora & Gabriela Castaño
In order to evaluate leaf litter Collembola at the tropical rainforest of Los Tuxtlas, Veracruz, México, fifteen pitfall traps were activated monthly for about one week, in a new experimental area during 2015. A total of 4,291 specimens were captured in the 180 samples collected. Ten families were recorded, those better represented were Paronellidae (27 %), Neanuridae (20.5 %), Entomobryidae (16.9 %), Dicyrtomidae (15 %), and Isotomidae (11.6 %). Among the 32 genera reported, most abundant were Trogolaphysa (18 %), Ptenothrix...
oxi-1 and fshr-1 are required for neuromuscular signaling under normal and oxidative stress conditions in C. elegansBarry Wei & Jennifer R Kowalski
Reactive oxygen species (ROS) contribute to neuronal degeneration by readily reacting with cellular components, consequently breaking down cellular integrity. Excess ROS often leads to oxidative stress, which results from destabilization of the organism's ability to control the balance between antioxidants and free radicals (Chandra et al. 2015). The ubiquitin-proteasome system helps to regulate oxidative stress and overall damage to cellular components by forming chains of ubiquitin polypeptides on cellular proteins; these chains then serve as...
Faunistics of oribatid mites (Acari, Oribatida) in dry grassland sites in the Eisack Valley (South Tyrol, Prov. Bolzano, Italy)Heinrich Schatz
Oribatid mites were investigated in five dry grassland sites in the basin around Klausen / Chiusa (South Tyrol). Soil and litter samples were taken from characteristic microhabitats (dry grassland, dry bushland, rocky steppe). A total of 150 oribatid species belonging to 47 families were encountered, most of them closely associated with dry habitats. Gymnodamaeus irregularis Bayartogtokh & Schatz, 2009 and Liochthonius perelegans Moritz, 1976 are new records for Italy, additional six species are new records...
Manuale tecnico descrittivo del software di gestione ed archiviazione dati astronomici chiamato New Archiving Distributed InfrastructuRe (NADIR).
Proprioseiopsis mauiensis (Prasad, 1968) and P. sharovi (Wainstein, 1975) are new species records for German mite fauna. They are re-described and illustrated.
The report describes the work done to deploy an Heterogeneous Computing Cluster using CPUs and GPUs integrated on a System on Chip architecture, in the environment of the ExaNeSt H2020 project. After deployment, this cluster will provide a testbed to validate and optimize several HPC codes developed to be run on heterogeneous hardware. The work done includes: a technology watching phase to choose the SoC best fitting our requirements; installation of the Operative System Ubuntu...
On the life cycle and parasitism of the trombiculid mite Hirsutiella hexasternalis (Kudryashova, 1998) (Acariformes, Trombiculidae)Andrey B. Shatrov
Post-larval life cycle, larval morphology and histology of the sites of the larvae feeding were investigated in the trombiculid mite Hirsutiella hexasternalis (Kudryashova, 1998) based on the material firstly collected from West Siberia (Tyumen province) from the voles Myodes rutilus (Pallas, 1779). Duration of the nymphal developmental stages generally corresponds to those of other trombiculid mites with the average values of 19 and 13 days for proto- and tritonymphs, respectively. Duration of active deutonymphal stage...
Distribution and diversity of earthworms (Lumbricidae) in Hesse (Central Germany): current knowledgeJörg Römbke, Wolfgang H. O. Dorow & Stephan Jänsch
In 2016 the first Red List of German earthworms was compiled, listing 46 species. However, little is known about the biodiversity and distribution of Lumbricidae in the individual federal states of Germany. In some of them (e.g. Schleswig-Holstein) earthworm monitoring programs were performed (or are still running). In others no such soil-focused program has been performed so far. The aim of this contribution is to compile the available information on the distribution of earthworms (Lumbricidae)...
Proper understanding of the reproductive biology traits and population dynamics patterns of declining songbird species is crucial for ensuring their effective protection and recovery. Metapopulation dynamics may cause the extinction of local populations in some landscape patches regardless of the habitat quality and undertaken conservation measures. At the same time, the source-sink type of the population dynamics could saturate lower quality habitat patches with dispersing individuals from the population sources. Hence, poorer quality habitats presumed...
A survey was carried out to find non-parasitic nematodes associated with slugs and snails in order to elucidate the initial phase of endophoresis and necromeny in gastropods. 78 % of the specimens of the 12 terrestrial gastropod species surveyed carried nematodes. A total of 23 nematode species were detected alive and propagable in gastropod faeces, with 16 different species found in Arion rufus alone. Most were saprobiontic rhabditids, with species of Caenorhabditis, Oscheius and Panagrolaimus appearing...
The various associations between nematodes and gastropods, which range from random transportation to endophoresis, necromeny, larval parasitism and an entire parasitic or parasitoidic life-style with and without intermediate or paratenic hosts, are discussed in an evolutionary context. Ten to eleven lineages have accomplished the transition to a life at the expense of gastropods, three via a detour of parasitism in vertebrates (Metastrongyloidea, Nemhelix and within Cosmocercoides). In the lineage to Agfa the sequence endophoretic, necromenic,...
Single nucleotide polymorphisms (SNPs) can be difficult to detect using traditional PCR and gel electrophoresis, especially when no enzyme restriction sites overlap the SNP. Although alleles can be identified through amplification with flanking primers and sequencing, this adds time and cost, and sequencing is typically outsourced. Tetra-primer Amplification-Refractory Mutation System (ARMS)-PCR allows SNPs to be distinguished through a 3' primer mismatch at the SNP site for one allele, and an additional mismatch 1 - 3...
Instytut Podstawowych Problemów Techniki PAN21
Journal «Theoretical and applied ecology»16
The Commonwealth Fund Website14
Société Française d'Ichtyologie13
Senckenberg Museum für Naturkunde Görlitz13
Department of Agriculture and Water Resources - ABARES11
Istituto Nazionale di Astrofisica8
Instytut Uprawy Nawożenia i Gleboznawstwa - Państwowy Instytut Badawczy3
Edith Cowan University1
Università degli Studi di Milano1
Plutof. Data Management and Publishing Platform1