142 Works

The yield of selected cultivars of spring barley depending on the sowing rate

Kazimierz Noworolnik, Danuta Leszczyńska & Piotr Kostiw

The influence of different doses of hydrogel on the quality of seeds and the yield of faba beans

Jerzy Księżak

A Mechanical Model of Heart Valves with Chordae for in Silico Real-Time Computations and Cardiac Surgery Planning

Gediminas GAIDULIS, Rimantas KAČIANAUSKAS, Natalya KIZILOVA & Yury ROMASHOV

Free in-plane and out-of-plane vibrations of rotating thin ring based on the toroidal shell theory

I. SENJANOVIĆ, I. ĆATIPOVIĆ, N. ALUJEVIĆ, D. ČAKMAK &

Material Spin and Finite-Strain Hypo-Elasticity for Two-Dimensional Orthotropic Media

Ivan Yurievitch ZUBKO

Torsion of Functionally Graded Anisotropic Linearly Elastic Circular Cylinder

István ECSEDI & Attila BAKSA

Database of Marine Larval Fish Assemblages in Australian temperate and subtropical waters (NIMO) 1983 - 2016

James A. Smith, Anthony G. Miskiewicz, Lynnath E. Beckley, Jason D. Everett, Valquiria Garcia, Charles A. Gray, David Holliday, Alan R. Jordan, John Keane, Ana Lara-Lopez, Jeffrey M. Leis, Paloma A. Matis, Barbara A. Muhling, Francisco J. Neira, Anthony J. Richardson, Kimberley A. Smith, Kerrie Swadling, Augy Syahailatua, Matthew D. Taylor, Paul D. Van Ruth, Tim M. Ward & Ian M. Suthers

Two new functions in the WormBase Enrichment Suite

David Angeles-Albores, Raymond Y.N. Lee, Juancarlos Chan & Paul W. Sternberg
​Genome-wide experiments routinely generate large amounts of data that can be hard to interpret biologically. A common approach to interpreting these results is to employ enrichment analyses of controlled languages, known as ontologies, that describe various biological parameters such as gene molecular or biological function. In C. elegans, three distinct ontologies, the Gene Ontology (GO), Anatomy Ontology (AO), and the Worm Phenotype Ontology (WPO) are used to annotate gene function, expression and phenotype, respectively (Ashburner...

SapTrap vectors for introducing point mutations with unc-119+ selection

Matthew L Schwartz & Erik M Jorgensen
SapTrap assembly generates CRISPR targeting vectors using a Golden Gate cloning strategy (Schwartz and Jorgensen, 2016). The original toolkit was designed for inserting tags, such as fluorescent proteins or affinity tags, into protein coding genes in C. elegans. In the original design, the floxed unc-119+ selectable marker was incorporated into the tag. For site-directed mutagenesis, the selectable marker is useful but a tag is unnecessary. We have developed a new set of vectors dedicated for...

Lecture on the anomalous diffusion in Condensed Matter Physics

M. Benhamou
Diffusion is a natural or artificial process that governs many phenomena in nature. The most known diffusion is the Brownian or normal motion, where the mean-square-displacement of the tracer (diffusive particle among others) increases as the square-root of time. It is not the case, however, for complex systems, where the diffusion is rather slow, because at small-scales, these media present an heterogenous structure. This kind of slow motion is called subdiffusion, where the associated mean-square-displacement...

The bodies of dpy-10(e128) are twice as stiff as wild type

Sylvia Fechner, Frédéric Loizeau, Adam L Nekimken, Beth L Pruitt & Miriam B Goodman
DPY-10 is a collagen protein in the nematode's cuticle. Mutations in the dpy-10 gene induce various morphological changes that lead to animals with a dumpy or roller phenotype (Levy, Yang & Kramer, 1993). Here, we asked how such mutations affect body stiffness by comparing force-indentation curves in dpy-10(e128) and wild type worms. On average, dpy-10(e128) worms have a steeper force-indentation relationship, leading to a higher body stiffness (Fig 1.C). Average stiffness values were 2.5 0.9...

A novel null allele of C. elegans gene ceh-14

Emily A. Bayer & Oliver Hobert
We have generated a novel null allele, ot900, of the C. elegans LIM homeodomain gene ceh-14. All existing deletion alleles of ceh-14 were generated using a Tc1 transposon insertion, one of which, ch3, is a putative null based on the resulting frame-shift and a reduction in detectable mRNA and protein levels (Cassata et al. 2000). However, in our hands we have noticed a propensity of the ch3 allele to revert to wild-type after several generations,...

Expression pattern of endogenous PAR-4A & C after CRISPR/Cas9-mediated genome editing

Vincent Roy, Olivier Gagné, Karim Hamiche, Jean-Claude Labbé & Patrick Narbonne
PAR-4/LKB1 is maternally expressed and required for the asymmetrical distribution of early embryonic determinants and viability in C. elegans (Morton et al. 1992; Watts et al. 2000; Tenlen et al. 2008). It is also implicated in a variety of postembryonic processes, including germline stem cell quiescence (Narbonne and Roy 2006; Narbonne et al. 2017), neuronal growth and polarity (Kim et al. 2010; Teichmann and Shen 2011), cytoskeletal rearrangements (Narbonne et al. 2010; Chartier et al....

Влияние содержания тяжёлых металлов в воде малых рек, используемой для полива кукурузы армянской популяции

А.Р. Сукиасян
Экологическая безопасность малых рек для населения региона, их роль в формировании биоты во многом определяются химическим составом воды. При изменении интенсивности действия климатических и антропогенных факторов химический состав и, как следствие, качество воды могут существенно изменяться. В связи с этим проведение мониторинговых исследований состава воды этих источников представляется весьма актуальным. Изучены особенности миграции ряда тяжёлых металлов (ТМ) в триаде вода-почва-растение на примере однолетнего травянистого культурного растения – сахарной кукурузы, произрастающего на прибрежных территориях реки Шнох....

Фитопоглощение радия-226 из техногенно загрязнённых почв на примере Chamaenerion angustifolium, Lathirus pratensis и L. vernus

Л. М. Шапошникова
Исследовано влияние физико-химического состава радиоактивно загрязнённых почв на поглощение радия-226 иван-чаем узколистным (Chamaenerion angustifolium) и чиной (Lathirus pratensis, L. vernus). Методами корреляционного и регрессионного анализа выявлено, что валовое содержание радия в исследуемых техногенных почвах больше всего зависит от содержания в них органического углерода и фосфора, содержание подвижных форм (ПФ) радионуклида связано с такими физико-химическими характеристиками как содержание органического углерода, а также обменных катионов кальция и магния. Межвидовых различий в накоплении радия указанными растениями выявлено не...

Applying songbird population dynamics models to conservation biology needs

A. L. Podolsky
Proper understanding of the reproductive biology traits and population dynamics patterns of declining songbird species is crucial for ensuring their effective protection and recovery. Metapopulation dynamics may cause the extinction of local populations in some landscape patches regardless of the habitat quality and undertaken conservation measures. At the same time, the source-sink type of the population dynamics could saturate lower quality habitat patches with dispersing individuals from the population sources. Hence, poorer quality habitats presumed...

Возможность частичной замены антибиотиков биологически активными веществами при лечении клинических маститов у коров

М. А. Азямов
По информации Всемирной организации здравоохранения (ВОЗ) возрастает проблема антибиотикорезистентных штаммов золотистого стафилококка Staphylococcus aureus. Эти бактерии меняют свой геномный состав под воздействием антибиотиков и быстро распространяются в окружающей среде, создавая угрозу для здоровья человечества и продовольственной безопасности ряда стран. ВОЗ требует уменьшить употребление антибиотиков при маститах у коров на 50%. Золотистый стафилококк – преобладающий вид возбудителя маститов коров в странах Западной Европы и России. Почти в 90% больших ферм и комплексов, где используют антибиотики, регистрируется...

Simplified detection of a point mutation in C. elegans using tetra-primer ARMS-PCR

Matthew T Sullenberger & Eleanor M Maine
Single nucleotide polymorphisms (SNPs) can be difficult to detect using traditional PCR and gel electrophoresis, especially when no enzyme restriction sites overlap the SNP. Although alleles can be identified through amplification with flanking primers and sequencing, this adds time and cost, and sequencing is typically outsourced. Tetra-primer Amplification-Refractory Mutation System (ARMS)-PCR allows SNPs to be distinguished through a 3' primer mismatch at the SNP site for one allele, and an additional mismatch 1 - 3...

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing

Leif Benner & Brian Oliver
A number of reagents have been generated for CRISPR/Cas9 genome editing in Drosophila (Gratz et al. 2014; Ren et al. 2013; Kondo and Ueda 2013; Port et al. 2014; Port and Bullock 2016). The ability to efficiently express multiple gRNAs from a single vector provides the advantage of creating multiple CRISPR/Cas9 alleles at a time. An elegant design has previously shown that gRNAs separated by tRNAs allow for genomic editing at multiple loci in Drosophila...

Прокариотические тест-модели в экотоксикологических исследованиях: перспективы и классификация (обзор)

Н.В. Дудчик, С.И. Сычик & В.В. Шевляков
Biological modeling in system ecology is an effective technology for assessing the biological effects of environmental factors. We provided the conceptual justification for the development of prokaryotic laboratory-analytical models, assessed the advantages and limitations of their experimental use. The classification of prokaryotic test models according to the level of the structural and ecological organization, the properties of the test object (cells, communities, populations, microbiocenosis) and a set of test reactions (bioindicators and biomarkers) is proposed....

First record of five fish species from Réunion Island observed during the inventory of the Marine Natural Zones of Interest for Ecology, Flora and Fauna (ZNIEFF)

Mathieu Pinault, Julien Wickel, Thierry Mulochau, Jean-Benoît Nicet & Ronald Fricke
This paper reports the first record of five species of marine fishes from Reunion Island, identified during biodiversity surveys carried out within the framework of the Marine Natural Zones of Interest for Ecology, Flora and Fauna (ZNIEFF). These species: Fusigobius inframaculatus(Randall, 1994), Fusigobius neophytus(Günther, 1877), Samariscus triocellatusWoods, 1960, Trichonotus marleyi(Smith, 1936) and Zapogon evermanni(Jordan and Snyder, 1904) were all photographed within non-reef habitats, despite the sampling of many reef and non-reef habitats of the island...

What can goby otolith morphology tell us?

Christoph Gierl, Dorothea Liebl, Radek Šanda, Jasna Vukić, Hamid Reza Esmaeili & Bettina Reichenbacher
The taxonomic information inscribed in otoliths has been widely ignored in ichthyological research, especially in descriptions of new fish species. One reason for this is that otolith descriptions are per se qualitative, and only a few studies have presented quantitative data that can support assignments of otoliths to individual species or permit differentiation between higher taxonomic levels. On the other hand, in palaeontology, otoliths have been employed for the identification and taxonomic placement of fossil...

Selectivity on epilithic diatom consumption for two tropical sympatric gobies: Sicydium punctatum Perugia, 1986 and Sicydium plumieri (Bloch, 1786)

Dominique Monti, Estelle Lefrançois, Clara Lord, Jean-Michel Mortillaro, Pascal Jean Lopez & Philippe Keith
Rivers of the Caribbean islands harbour a freshwater fauna mainly constituted of migrant diadromous species. In these hyperturbulent ecosystems, the primary producers are mostly represented by a thin epilithic biofilm, mainly composed of diatoms. Comparison of the diatoms available from the environment with the ones consumed and located in the digestive tracts of two gobiid fish, Sicydium punctatum Perugia, 1896 and Sicydium plumieri (Bloch, 1786) were made at twelve sampling locations, located upstream or downstream...

oxi-1 and fshr-1 are required for neuromuscular signaling under normal and oxidative stress conditions in C. elegans

Barry Wei & Jennifer R Kowalski
Reactive oxygen species (ROS) contribute to neuronal degeneration by readily reacting with cellular components, consequently breaking down cellular integrity. Excess ROS often leads to oxidative stress, which results from destabilization of the organism's ability to control the balance between antioxidants and free radicals (Chandra et al. 2015). The ubiquitin-proteasome system helps to regulate oxidative stress and overall damage to cellular components by forming chains of ubiquitin polypeptides on cellular proteins; these chains then serve as...

Nuclear localization of a C. elegans CCCH-type zinc finger protein encoded by T26A8.4

Takashi Koyama & Chisato Ushida
T26A8.4 encodes a CCCH-type zinc finger protein. The amino acid sequence of this protein is partially similar to that of S. cerevisiae Caf120p, which has no zinc finger domain. T26A8.4-encoded RNA was detected more abundantly in germ cells than in other tissues of L4-adult worms according to the NEXTDB data. When expressed specifically in the germline under control of the mex-5 promoter, the T26A8.4-encoded protein localized to germ cell nuclei in the adult hermaphrodite and...

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