142 Works

Thermal Instability Analysis of an Elastico-Viscous Nanofluid Layer

Ramesh CHAND, Gian C. RANA & Dolors PUIGJANER

Evaluation of Cutting Forces and Temperature of Iron-Rich Binder Carbide Tool in Turning of Titanium

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Experimental Verification of the Steering Performance of All-Hydraulic Crawler Chassis

Xiaolian LV, Xiaorong LV, Xiaojie SHI & Tingyu WANG

The Commonwealth Fund’s Top 10 for 2018

Targeting Phage and Antibiotic Resistance 2018

The 5th World Congress on Targeting Infectious Diseases: Targeting Phage & Antibiotic Resistance 2018 was organized in Florence, Italy in May 17-18, 2018. During two days, more than 100 oral & poster communications were highlighted different axes and topics related to the recent advances and challenges in phage therapy, and to all innovations related to the antibiotic resistance in general. The 5th edition was an excellent platform which gathered more than 200 participants from 33...

Agricultural commodity statistics 2018

Australian forest and wood products statistics: March and June quarters 2018

This issue of the Australian forest and wood products statistics (AFWPS) presents 2016-17 data for key indicators of domestic forestry sector activity, including sales and service income and industry value added. It includes estimates of the volume and value of logs harvested in 2017-18; as well as housing and other residential commencements, and wood products trade statistics for 2017-18. This AFWPS issue also presents updated socio-economic indicators and trends for the forestry sector drawn from...

SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette

Daniel J. Dickinson, Mark M. Slabodnick, Alicia H. Chen & Bob Goldstein
The advent of CRISPR/Cas9 technology in C. elegans has enabled an unprecedented level of control over this organism’s genome, which is facilitating research in a wide variety of fields. Several different experimental approaches exist for CRISPR in this organism (Dickinson and Goldstein 2016). One particularly powerful approach uses a drug selection with a Self-Excising Cassette (SEC) to isolate the desired genetically-modified animals following Cas9-triggered homologous recombination (Dickinson et al. 2015). The most labor-intensive step in...

Efficient FLP-mediated germ-line recombination in C. elegans

Javier Macías-León & Peter Askjaer
​Recombinases are very useful enzymes for spatiotemporal control of gene expression (Hubbard 2014). Through binding to specific DNA sequences, they can be used to excise or exchange DNA fragments. We have previously generated a series of Caenorhabditis elegans strains that stably express an efficient FLP recombinase in specific tissues and reported how they enable precise regulation of gene expression, cell ablation or conditional gene knockout (Muñoz-Jiménez et al 2017). However, these strains are restricted to...

GFP::PCN-1 does not reliably mark S phase in C. elegans adult germline progenitor zone cells

Tokiko Furuta & Swathi Arur
PCNA (proliferating cell nuclear antigen) is the DNA polymerase processivity factor that loads onto the chromatin during S phase of the cell-cycle (Brauchle et al. 2003). Thus, nuclear localization of PCNA (PCN-1 in C. elegans) is used as a marker for the S phase of the cell cycle (Brauchle et al. 2003). GFP::PCN-1 has been shown to label S phase in C. elegans embryo when driven through the germline and embryonic promoter pie-1 (Brauchle et...

Silencing the ASI gustatory neuron pair extends lifespan

Peter Chisnell & Cynthia Kenyon
Disrupting the function of sensory neurons of C. elegans can increase their lifespan (Apeld and Kenyon 1999). This effect is not limited to large-scale disruption, as ablation of single pairs of neurons have been shown to modify lifespan (Alcedo and Kenyon 2004; Lee and Kenyon 2009; Liu and Cai 2013). We tested whether silencing the neuron pair ASI with the tetanus toxin light chain (Tetx), as opposed to ablating it, could increase lifespan. Tetanus toxin...

Dominant-negative VPS-4 disrupts ODR-10::GFP distribution but has limited effects on chemotaxis

Ellen Zocher, Nelson Ruth & Caroline Dahlberg
Accumulation of ODR-10 depends on the sex and fed state of C. elegans (Ryan et al. 2014), though the precise mechanism of its regulation is not known. We tested whether the ESCRT pathway is required to maintain normal accumulation and distribution of ODR-10::GFP in AWA neurons under well-fed and starved conditions. The conserved AAA ATPase VPS-4 is required as the final step of the ESCRT pathway, to dissociate the machinery as cargo is moved from...

Endocannabinoid 2-arachidonoyl glycerol increases the transcription of daf-7 in ASI neurons

Celina Galles, Gastón M Prez & Diego De Mendoza
daf-7 expression in ASI neurons is increased when nematodes are grown in the presence of endocannabinoid 2-arachidonoyl glycerol (2-AG). Left upper panel: representative image of reporter strain FK181 ksIs2 [Pdaf-7::GFP+rol-6(su1006)] captured under GFP epifluorescence microscopy. Right upper panel: same individual showing bright field microscopy together with GFP signal. Gray/black triangles mark ASI neurons expressing daf-7. Scale bars correspond to 0.03 mm. Lower panel: point plot of the adjusted GFP intensity/ASI neuron values gathered from worms...

2018 Update on Protein-Protein Interaction Data in WormBase

Jaehyoung Cho, Christian Grove, Kimberly Van Auken, Juancarlos Chan, Sibyl Gao & Paul W. Sternberg
Protein interaction is an important data type to understand the biological function of proteins involved in the interaction, and helps researchers to deduce the biological nature of unknown proteins from the well-characterized functions of their interaction partners. High-throughput studies, coupled with the aggregation of individual experiments, provides a global 'snapshot' of the protein interactions occurring at all levels of biological processes or circumstances. This snapshot of the interaction network, the interactome, is important to understand...

Impact of forest fire on soil properties (review)

A.A. Dymov, E.V. Abakumov, I.N. Bezkorovaynaya, A.S. Prokushkin, Ya.V. Kuzyakov & E.Yu. Milanovsky
We examined changes in the morphological, physicochemical properties and features of the organic matter of forest soils impacted by wildfires on the territory of Russia. Morphological signs of pyrogenesis (pyrogenic horizon formation, partial charring of litter and illuviation of organic compounds) are most evident detected in the first decade after a fire. Ground fires in lichen pine forests, formed on Albic Podzols lead to complete burning of litter. Low intensity ground fires in sphagnum pine...

A new deletion allele of sma-4

Alexandra N. McKillop, Herong Shi & Jun Liu
sma-4 encodes the co-Smad of the BMP pathway in C. elegans, which is also known as the Sma/Mab pathway (Savage et al. 1996). Null mutations in core components of this pathway, including the BMP ligand DBL-1, the receptors SMA-6 and DAF-4, and the R-Smads SMA-2 and SMA-3, all result in a small body size phenotype without significantly compromising viability (Gumienny and Savage-Dunn 2013). However, we found that even after multiple rounds of out-crossing, two deletion...

The Mapping of Drosophila melanogaster mutant A.4.4

Kayla L Bieser, Joyce Stamm, Ayala A Aldo, Suneil Bhaskara, Makayla Claiborne, Joselyn N Coronel Gómez, Ron Dean, Aaron Dowell, Evan Dowell, Mathew Eissa, Ahmad A Fawaz, Michael M Fouad-Meshriky, Dustin Godoy, Krista Gonzalez, Malak K Hachem, Malak F Hammoud, Anthony Huffman, Hunter Ingram, Alex B Jackman, Bibek Karki, Natalia Khalil, Houda Khalil, Tran Khanh Ha, Arjun Kharel, Izabell Kobylarz … & Jacob D Kagey
A novel Drosophila melanogaster mutant A.4.4 was isolated from a conditional Flp/FRT mosaic eye screen in the context of blocked apoptosis (Kagey et al., 2012). The ;FRT42D, Dark82 chromosome was used as a starting point for the EMS mutagenesis screen to screen for mutations that conferred a growth advantage in the environment of blocked apoptosis via the homozygous Dark82 allele (Akdemir et al., 2006). Mutants were screened for over-representation of mutant tissue (pigmented) as compared...

Agricultural commodities: December quarter 2018

The December edition of Agricultural commodities contains ABARES latest outlook for Australia's key agricultural commodities in 2018-19. This release is an update to the outlook released in March 2018.

Комбинированные расчётные схемы в математических моделях процессов фракционирования зерна

В.А. Сысуев, А.В. Алёшкин, Ю.В. Сычугов & А.Ю. Исупов
Сельскохозяйственная экология предлагает новые методы и подходы к осуществлению производственных процессов, которые позволят снизить вредное влияние на окружающую среду. В статье изложены подходы к формированию комбинированной расчётной схемы процесса фракционирования зерновых материалов воздушным потоком в горизонтальном канале. Основной идеей метода является последовательное уточнение взаимного влияния зернового и воздушного потоков при расчёте поля скоростей воздуха и траекторий дисперсных частиц. Математическая модель движения воздушного потока построена на основе решения методом конечных элементов уравнений Лапласа для плоского потенциального...

Исследование процессов миграции и трансформации смазочно-охлаждающих жидкостей в почве урбанизированной территории

Л.М. Щуклина, Г.М. Батракова & Т.В. Нурисламова
Актуальность работы определяется требованиями экологической безопасности при строительстве на территориях с выявленным техногенным загрязнением почв. В работе рассматривается применение хроматомасс-спектрометрии в исследовании загрязнённого грунта с целью идентификации продуктов трансформации в природных условиях многокомпонентных рецептур смазочно-охлаждающих жидкостей. Выполнена идентификация и поиск по масс-спектрам компонентного состава продуктов трансформации индустриального масла, предложено маркерное соединение. Показана возможность идентификации на основании регистрации масс-спектров ионов химических соединений, определена структура и элементный состав. Поиск характеристических ионов органических соединений выполнялся с помощью банка...

Получение экологически безопасных строительных материалов с использованием осадков сточных вод целлюлозно-бумажного производства

Е.С. Ширинкина
В работе представлены исследования в области использования осадка сточных вод целлюлозно-бумажной промышленности (ЦБП) в качестве ресурса при производстве строительных материалов. В ходе анализа научнотехнической информации было установлено, что осадок сточных вод ЦБП, содержащий в своём составе целлюлозное волокно (скоп) и активный ил, может применяться в качестве добавки при производстве композитных материалов, теплоизоляционных плит, лёгких заполнителей для бетонов и в качестве выгорающей добавки при производстве керамического кирпича. В лабораторных условиях исследовали возможность получения керамического кирпича с...

Specimen occurrence data from Lauri Mällo private collection

Lauri Mällo
Specimen occurrence data of Lauri Mällo Lepidoptera collection

Influence of Rotation on Transversely Isotropic Piezoelectric Rod Coated with a Thin Film

Rajendran SELVAMANI & Oluwole Daniel MAKINDE

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