Poststroke depression (PSD) is a common complication that seriously affects the functional recovery and prognosis of an individual. As some patients with PSD fail to respond to drug therapy, it is urgent to find a viable alternative treatment. An active exercise program known as foraging exercise (FE), using food as bait, was designed. First, focal ischemia and chronic unpredictable mild stress (CUMS) were used to establish a PSD model in rats. FE was then performed...

Osteosarcoma (OS) is the most common primary malignant bone tumor. Prospero homeobox 1 (PROX1) is a key transcription factor involved in some cancers, but the role of PROX1 in OS is unclear. This study aimed to explore the clinical and biology significance of PROX1 in OS. Fifty-four OS tissues and matched nontumor tissues were collected to explore the relationship between PROX1 expression and clinical characteristics and prognosis. qRT-PCR and immunohistochemistry were used to investigate the...

Liver injury from any number of causes (e.g. chemical material, drugs and diet, viral infection) is a global health problem, and its mechanism is not clearly understood. MicroRNAs (miRNAs) expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and have also shown promise as biomarkers for disease. Previous studies reported the regulation effects of miRNAs in liver injury, whereas function and molecular mechanisms of miR-322-5p...

Patients with post-traumatic stress disorder (PTSD) are usually at an increased risk for chronic disorders, such as irritable bowel syndrome (IBS), characterized by hyperalgesia and allodynia, but its subsequent effect on visceral hyperalgesia and the mechanism remain unclear. The present study employed single prolonged stress (SPS), a model of PTSD-pain comorbidity, behavioral evaluation, intrathecal drug delivery, immunohistochemistry, Western blotting, and RT-PCR techniques. When detecting visceral sensitivity, the score of the abdominal withdrawal reflex (AWR) induced...

Sevoflurane is identified as an effective candidate drug for acute lung injury (ALI) treatment, but its curing effects and detailed mechanisms have not been fully disclosed. The present study was designed to resolve this academic issue. The ALI mice models were established, and Hematoxylin-eosin staining assay was performed to examine tissue morphologies. Cell viability was determined by CCK-8 assay, and Annexin V-FITC/PI double staining assay was used to examine cell apoptosis. The expression levels of...

Background: Several studies scatteredly identified the myelodysplastic syndromes’ transcriptomic profiles (MDS). However, the exploration of transcriptional signatures, key signalling pathways, and their association with prognosis and diagnosis in the integrated multiple datasets remains lacking. Methods: We integrated the GSE4619, GSE19429, GSE30195, and GSE58831 microarray datasets of CD34 + cells for identifying the differentially expressed genes (DEGs) in the MDS. The series of bioinformatics methods are applied to identify the key hub genes, gene clusters, prognostic...

High-grade serous ovarian cancer (HGSOC) is the most common and malignant type of ovarian cancer, accounting for 70%–80% of mortality. However, the treatment of HGSOC has improved little in the past few decades. Metformin is the first-line medication for the treatment of type 2 diabetes and has now gained more attention in cancer treatment. In this study, we sought to identify potential hub genes that metformin could target in the treatment of HGSOC. We downloaded...

Combining individual p-values to perform an overall test is often encountered in statistical applications. The Cauchy combination test (CCT) (Journal of the American Statistical Association, 2020, 115, 393–402) is a powerful and computationally efficient approach to integrate individual p-values under arbitrary dependence structures for sparse signals. We revisit this test to additionally show that (i) the tail probability of the CCT can be approximated just as well when more relaxed assumptions are imposed on individual...

Additional file 1. Table S1: 811 genes of a genetic skeletal disease panel; Table S2: Primer sequence in this study; Table S3: Summary of clinical information and molecular results for 55 cases with skeletal abnormalities.

Additional file 7: Table S7. Differential lipids related DEGs (TOP 500).

Additional file 3: Table S3. Codon usage and relative synonymous codon usage (RSCU) values of protain-coding genes of the 16 Sorbus s.s. chloroplast genomes

Additional file 7: Table S7. Information of species included in phylogenetic analyses

Mesquite readable file in NEXUS format

Additional file 20. Photoperiod experiment and FMT experiment_data.

Additional file 21. Rcode.R.

Nucleomodulins are secreted bacterial proteins whose molecular targets are located in host cell nuclei. They are gaining attention as critical virulence factors that either modify the epigenetics of host cells or directly regulate host gene expression. Mycoplasma bovis is a major veterinary pathogen that secretes several potential virulence factors. The aim of this study was to determine whether any of their secreted proteins might function as nucleomodulins. After an initial in silico screening, the nuclear...

The West Junggar terrain (WJT), as a crucial part of the Central Asian Orogenic Belt (CAOB), is distributed with numerous igneous rocks, which provide critical information for crustal growth. However, the closure of the Junggar Ocean (JO) and the beginning of the postcollisional tectonic stage of the WJT have been controversial. This study delimited the regional lithologic units based on remote sensing geological mapping and recognized a series of bimodal volcanic rocks (BVR) in Hala’alate...

The West Junggar terrain (WJT), as a crucial part of the Central Asian Orogenic Belt (CAOB), is distributed with numerous igneous rocks, which provide critical information for crustal growth. However, the closure of the Junggar Ocean (JO) and the beginning of the postcollisional tectonic stage of the WJT have been controversial. This study delimited the regional lithologic units based on remote sensing geological mapping and recognized a series of bimodal volcanic rocks (BVR) in Hala’alate...

This study aimed at investigating the relationships between Platelet-Lymphocyte ratio (PLR) and Neutrophil–Lymphocyte ratio (NLR) and their dynamic changes (∆PLR, ∆NLR) with type 2 diabetes mellitus (T2DM) in a Chinese cohort study. This study recruited 41,439 individuals who were diagnosed without T2DM at first health examination and completed at least one follow-up. The relationships between NLR, PLR, ∆PLR, ∆NLR and T2DM risk were analyzed using the Cox regression model with corresponding Hazard Ratios (HRs) and...

Additional file 2: Table S1. Differential lncRNAs between TOF and control groupincluding 21 up- and 73 down-regulated lncRNAs. Table S2. Differential mRNAs between TOF and control groupincluding 41 up- and 42 down-regulated genes. Table S3. lncRNA correlations to mRNA showed difference betweenTOF and control group.

Comma separated character taxon matrix of tube phenotype characters

NEXUS file formatted for MrBayes with commands necessary to execute the analyses

Additional file 7: Table S1. The comparison data of the two genomes.

Supplemental material, sj-xls-1-cix-10.1177_11769351221139252 for Identifying the Therapeutic and Prognostic Role of the CD8+ T Cell-Related Gene ALDH2 in Head and Neck Squamous Cell Carcinoma by Hongmei Zhang, Zhaozheng Li and Yan Zheng in Cancer Informatics

Bats are reservoir hosts for various zoonotic viruses with pandemdic potential in humans and livestock. In vitro systems for studying bat host–pathogen interactions are of significant interest. Here, we establish protocols to generate bat airway organoids (AOs) and airway epithelial cells differentiated at the air–liquid interface (ALI-AECs) from tracheal tissues of the cave-nectar bat Eonycteris spelaea. In particular, we describe steps which enable laboratories that do not have access to live bats to perform extended...