187 Works

Alkannin restrains oral squamous carcinoma cell growth, migration and invasion by regulating microRNA-9/RECK axis

Yulong Mao, Weiwei Zhang, Ronghe Zhang & Jinhua Zuo
Alkannin (ALK) has anti-inflammatory and anti-tumour activities. We tried to probe the underlying functions of ALK in oral squamous carcinoma (OSCC) cells growth, migration and invasion. OSCC cells viability was investigated after treatment with ALK. Then, BrdU, flow cytometry, Western blot and Transwell assays were executed to appraise proliferation, apoptosis, migration and invasion in OSCC cells with ALK stimulation. The biological functions of miR-9 were explored after miR-9 mimic/inhibitor transfection. The relevance of RECK and...

The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity

Xuemeng Ji, Ping Lu, Juan Xue, Ning Zhao, Yan Zhang, Lu Dong, Xuejiao Zhang, Ping Li, Yaozhong Hu, Jin Wang, Bowei Zhang, Jingmin Liu, Huan lv & Shuo Wang
Cronobacter sakazakii, an emerging opportunistic pathogen, is implicated in severe foodborne outbreak infections in premature and full-term infants. Generally, acid tolerance is vital for the pathogenesis of foodborne pathogens; however, its role in C. sakazakii virulence remains largely unknown. To screen out acid-tolerance determinants from transposon mutants, anovel counterselection method using gentamicin and acid was developed. Using the counterselection method and growth assay, we screened several acid-sensitive mutants and found that nlpD encodes an acid-resistance...

Preventing Candida albicans from subverting host plasminogen for invasive infection treatment

Si-Min Chen, Zui Zou, Shi-Yu Guo, Wei-Tong Hou, Xi-Ran Qiu, Yu Zhang, Li-Jun Song, Xin-Yu Hu, Yuan-Ying Jiang, Hui Shen & Mao-Mao An
Candida albicans is a common fungal pathogen in humans that colonizes the skin and mucosal surfaces of the majority healthy individuals. How C. albicans disseminates into the bloodstream and causes life-threatening systemic infections in immunocompromised patients remains unclear. Plasminogen system activation can degrade a variety of structural proteins in vivo and is involved in several homeostatic processes. Here, for the first time, we characterized that C. albicans could capture and “subvert” host plasminogen to invade...

The expression and clinical prognostic value of protein phosphatase 1 catalytic subunit beta in pancreatic cancer

Lingyu Hu, Haokai Xu, Xiaoguang Wang, Bin Wu, Fei Chen, Wei Chen, Yong Gao & Zhengxiang Zhong
Pancreatic cancer (PAAD) is a common malignancy with a poor survival rate. The identification of novel biomarkers could improve clinical outcomes for patients with PAAD. Here we evaluated the expression and clinical significance of PPP1CB in PAAD. PPP1CB expression was higher in PAAD tissue than in matched paracancerous tissue (P < 0.05). We predicted a network of regulatory targets and protein interaction partners of PPP1CB, and identified a PPI network consisting of 39 node genes....

Functional convergence of gliP and aspf1 in Aspergillus fumigatus pathogenicity

Hong Liu, Wenjie Xu, Norma V. Solis, Carol Woolford, Aaron P. Mitchell & Scott G. Filler
Gliotoxin contributes to the virulence of the fungus Aspergillus fumigatus in non-neutropenic mice that are immunosuppressed with corticosteroids. To investigate how the absence of gliotoxin affects both the fungus and the host, we used a nanoString nCounter to analyze their transcriptional responses during pulmonary infection of a non-neutropenic host with a gliotoxin-deficient ΔgliP mutant. We found that the ΔgliP mutation led to increased expression of aspf1, which specifies a secreted ribotoxin. Prior studies have shown...

Circular RNA hsa_circ_0032683 inhibits the progression of hepatocellular carcinoma by sponging microRNA-338-5p

Hao Shen, Haifeng Li & Jiahua Zhou
Recently, several studies have been conducted on circRNA (circular RNA). circRNA regulates gene expression and plays a vital role in the occurrence and development of various tumors. However, the role and mechanism of hsa_circ_0032683 in hepatocellular carcinoma (HCC) is not studied yet. In GEO (Gene Expression Omnibus) database, hsa_circ_0032683 expression was significantly lower in HCC tissues than in normal liver tissues. In vitro and in vivo functional tests revealed that hsa_circ_0032683 could inhibit HCC cells...

N(6)-adenosine-methyltransferase-14 promotes glioma tumorigenesis by repressing argininosuccinate synthase 1 expression in an m6A-dependent manner

You-Qing Miao, Wei Chen, Jianfeng Zhou, Qiyang Shen, Ying Sun, Tao Li & Sheng-Chan Wang
Glioma is one of the leading causes of tumor-related deaths worldwide, but its potential mechanism remains unclear. This study aimed to explore the biological role and potential mechanism of argininosuccinate synthase 1 (ASS1) in glioma. The relative expression levels of ASS1 in glioma specimens and cell lines were calculated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. The biological functions of ASS1 were demonstrated using the 5-ethynyl-2ʹ-deoxyuridine (EdU) assay, transwell assay, and in...

Zinc finger C3H1 domain-containing protein (ZFC3H1) evaluates the prognosis and treatment of prostate adenocarcinoma (PRAD): A study based on TCGA data

Hang Huang, Haokai Xu, Ping Li, Xueting Ye, Wei Chen & Xixi Huang
The present study was aimed to evaluate the expression profile of Zinc finger C3H1 domain-containing protein (ZFC3H1) using bioinformatic analysis of public datasets from The Cancer Genome Atlas database (TCGA). The results showed that the expression levels of ZFC3H1 were notably lower than the corresponding non-cancerous tissues in prostate adenocarcinoma (PRAD), and patients in the high ZFC3H1-expression group showed poor survival. We hypothesized that the low expression of ZFC3H1 in tumor tissue might have be...

Genome-wide identification and expression pattern analysis of the TCP transcription factor family in Ginkgo biloba

Li Yu, Qiangwen Chen, Jiarui Zheng, Feng Xu, Jiabao Ye, Weiwei Zhang, Yongling Liao & Xiaoyan Yang
Plant-specific TCP transcription factors play an essential role in plant growth and development. They can regulate leaf curvature, flower symmetry and the synthesis of secondary metabolites. The flavonoids in Ginkgo biloba leaf are one of the main medicinally bioactivate compounds, which have pharmacological and beneficial health effects for humans. In this study, a total of 13 TCP genes were identified in G. biloba, and 5 of them belonged to PCF subclades (GbTCP03, GbTCP07, GbTCP05, GbTCP13,...

miR-199a-3p plays an anti-tumorigenic role in lung adenocarcinoma by suppressing anterior gradient 2

Hui Liu, Yanfeng Wang, Yi Wang, Daoyuan Wu & He Zhang
Previous studies have explored the association between protein-coding genes and microRNAs (miRNAs) in lung adenocarcinoma (LUAD). However, the influence of the miR-199a-3p/anterior gradient 2 (AGR2) axis in LUAD has not yet been fully explored. Therefore, this study aimed to examine the underlying roles of AGR2 and miR-199a-3p in the development of LUAD. The expression levels of miR-199a-3p and AGR2 in LUAD tissues and cells were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). A luciferase...

Additional file 1 of A CT-based nomogram for differentiating invasive fungal disease of the lung from bacterial pneumonia

Meilin Gong, Jingmei Xu, Kang Li, Ke Li, Yuwei Xia, Yang Jing, Jiafei Chen, Jing Li, Jing Yang, Mingshan Du, Wenjing Hou, Yuan Ou, Lian Li & Wei Chen
Additional file 1. On the orininal data of the study.

Additional file 3 of Efficacy and safety of Tripterygium wilfordii polyglycosides for diabetic kidney disease: an overview of systematic reviews and meta-analyses

Ying Wang, Mei Han, Yao-Tan Li, Zhen Wang & Jian-Ping Liu
Additional file 3: Supplemental Table 1. Overview of 24-hour Urinary Protein in the Included SRs and MAs. Supplemental Table 2. Overview of the Included SRs and MAs of Renal Function. Supplemental Table 3. Overview of the Included SRs and MAs about the Outcome of Serum Albumin. Supplemental Table 4. Overview of the Included SRs and MAs of AL. Supplemental Table 5. Overview of the included SRs and MAs about the outcomes of WBC. Supplemental Table...

Additional file 2 of Transcriptome analysis of MYB transcription factors family and PgMYB genes involved in salt stress resistance in Panax ginseng

Mingming Liu, Ke Li, Shichao Sheng, Mingyu Wang, Panpan Hua, Yanfang Wang, Ping Chen, Kangyu Wang, Mingzhu Zhao, Yi Wang & Meiping Zhang
Additional file 2: Table S2. The PgMYB protein sequences for phylogenetic analysis.

MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha

Gang Chen, Qihao Wang & Kunyu Wang
Lung adenocarcinoma (LUAD) severely threatens the health of people owing to its lethality. Nonetheless, the underlying mechanisms on LUAD development remain unclear to a great extent. This work aimed to probe the functions of miR-218-5p in LUAD. MiR-218-5p and endoplasmic reticulum oxidoreductase 1 alpha (ERO1A) were screened as differently downregulated and upregulated RNAs in LUAD, respectively, by bioinformatics analyses. The results of cell functional assays stated that enforced expression of miR-218-5p notably restrained cell viability,...

The efficacy and safety of cyclosporine A plus androgen versus androgen alone for adult patients with non-severe aplastic anemia in China: a meta-analysis of randomized controlled trials

Xiao-tian Zhang, Ya-nan Zhang, Jing-jing Zhu, Xue Wang, Jiang Cao, Wei Chen, Na Qi, Kai-lin Xu & Hai Cheng
Whether combined CsA with androgen therapy was superior to androgen therapy alone in NSAA remains controversial. This study aimed to assess the efficacy and safety of combined therapy versus androgen therapy for NSAA patients using a meta-analytic approach. An electronic database of PubMed, EmBase, Cochrane library, CNKI, VIP, and Wanfang was systematically searched for randomized controlled trials (RCTs) from their inception to February 2020. The primary endpoint was effective rate, while the secondary endpoints included...

Comparative transcriptomic analysis reveal genes involved in the pathogenicity increase of Streptococcus suis epidemic strains

Jianping Wang, Pujun Liang, Hui Sun, Zongfu Wu, Marcelo Gottschalk, Kexin Qi & Han Zheng
Streptococcus suis epidemic strains were responsible for two outbreaks in China and possessed increased pathogenicity which was featured prominently by inducing an excessive inflammatory response at the early phase of infection. To discover the critical genes responsible for the pathogenicity increase of S. suis epidemic strains, the genome-wide transcriptional profiles of epidemic strain SC84 were investigated at the early phase of interaction with BV2 cells. The overall low expression levels of 89K pathogenicity island (PAI)...

Additional file 11 of Transcriptome analysis reveals the roles of phytohormone signaling in tea plant (Camellia sinensis L.) flower development

Xiaohan Xu, Jing Tao, Anqi Xing, Zichen Wu, Yuqin Xu, Yi Sun, Jiangyuan Zhu, Xiang Dai & Yuhua Wang
Additional file 11: Table S8: Two-way ANOVA test F-value of genes expression levels related to abscisic acid biosynthesis and signaling transduction for three C. sinensis cultivars (BY1, HJY and SCZ) affected by development stage (S) and cultivar (C).

Additional file 8 of Transcriptome analysis reveals the roles of phytohormone signaling in tea plant (Camellia sinensis L.) flower development

Xiaohan Xu, Jing Tao, Anqi Xing, Zichen Wu, Yuqin Xu, Yi Sun, Jiangyuan Zhu, Xiang Dai & Yuhua Wang
Additional file 8: Table S5: Two-way ANOVA test F-value of expression levels of the genes related to flowering time and flower development for three C. sinensis cultivars (BY1, HJY and SCZ) affected by development stage (S) and cultivar (C).

Additional file 9 of Transcriptome analysis reveals the roles of phytohormone signaling in tea plant (Camellia sinensis L.) flower development

Xiaohan Xu, Jing Tao, Anqi Xing, Zichen Wu, Yuqin Xu, Yi Sun, Jiangyuan Zhu, Xiang Dai & Yuhua Wang
Additional file 9: Table S6: Two-way ANOVA test F-value of genes expression levels related to auxin biosynthesis and signaling transduction for three C. sinensis cultivars (BY1, HJY and SCZ) affected by development stage (S) and cultivar (C).

Additional file 1 of Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining

Fang-Cheng Jiang, Jia-Yuan Luo, Yi-Wu Dang, Hui-Ping Lu, Dong-Ming Li, Zhi-Guang Huang, Yu-Lu Tang, Ye-Ying Fang, Yu-Xing Tang, Ya-Si Su, Wen-Bin Dai, Shang-Ling Pan, Zhen-Bo Feng, Gang Chen & Juan He
Additional file 1: Table S1. Raw data for IHC staining.

Additional file 4 of Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining

Fang-Cheng Jiang, Jia-Yuan Luo, Yi-Wu Dang, Hui-Ping Lu, Dong-Ming Li, Zhi-Guang Huang, Yu-Lu Tang, Ye-Ying Fang, Yu-Xing Tang, Ya-Si Su, Wen-Bin Dai, Shang-Ling Pan, Zhen-Bo Feng, Gang Chen & Juan He
Additional file 4: Table S4. Gene list of positively and differentially co-expressed genes of ZNF71.

Additional file 5 of Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining

Fang-Cheng Jiang, Jia-Yuan Luo, Yi-Wu Dang, Hui-Ping Lu, Dong-Ming Li, Zhi-Guang Huang, Yu-Lu Tang, Ye-Ying Fang, Yu-Xing Tang, Ya-Si Su, Wen-Bin Dai, Shang-Ling Pan, Zhen-Bo Feng, Gang Chen & Juan He
Additional file 5: Table S5. Pearson correlation analysis between ZNF71 and its putative target genes which relates to tight junction.

Additional file 1 of Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)

Gaohui Zha, Juan Yin, Feng Cheng, Mengfei Song, Mengru Zhang, Hesbon Ochieng Obel, Yi Wang, Jinfeng Chen & Qunfeng Lou
Additional file 1.

Decreased echinocandin susceptibility in Candida parapsilosis causing candidemia and emergence of a pan-echinocandin resistant case in China

Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun & Li Zhang
Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Functional convergence of gliP and aspf1 in Aspergillus fumigatus pathogenicity

Hong Liu, Wenjie Xu, Norma V. Solis, Carol Woolford, Aaron P. Mitchell & Scott G. Filler
Gliotoxin contributes to the virulence of the fungus Aspergillus fumigatus in non-neutropenic mice that are immunosuppressed with corticosteroids. To investigate how the absence of gliotoxin affects both the fungus and the host, we used a nanoString nCounter to analyze their transcriptional responses during pulmonary infection of a non-neutropenic host with a gliotoxin-deficient ΔgliP mutant. We found that the ΔgliP mutation led to increased expression of aspf1, which specifies a secreted ribotoxin. Prior studies have shown...

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