22 Works

Data from: A large fraction of HLA class I ligands are proteasome-generated spliced peptides

Juliane Liepe, Fabio Marino, John Sidney, Anita Jeko, Daniel E. Bunting, Alessandro Sette, Peter M. Kloetzel, Michael P. H. Stumpf, Albert J. R. Heck & Michele Mishto
The proteasome generates the epitopes presented on human leukocyte antigen (HLA) class I molecules that elicit CD8+ T cell responses. Reports of proteasome-generated spliced epitopes exist, but they have been regarded as rare events. Here, however, we show that the proteasome-generated spliced peptide pool accounts for one-third of the entire HLA class I immunopeptidome in terms of diversity and one-fourth in terms of abundance. This pool also represents a unique set of antigens, possessing particular...

Minor neuropsychological deficits in patients with subjective cognitive decline

Steffen Wolfsgruber, Luca Kleineidam, Jannis Guski, Alexandra Polcher, Ingo Frommann, Sandra Roeske, Eike Jakob Spruth, Christina Franke, Josef Priller, Ingo Kilimann, Stefan Teipel, Katharina Buerger, Daniel Janowitz, Christoph Laske, Martina Buchmann, Oliver Peters, Felix Menne, Manuel Fuentes Casan, Jens Wiltfang, Claudia Bartels, Emrah Düzel, Coraline Metzger, Wenzel Glanz, Manuela Thelen, Annika Spottke … & Michael Wagner
Objective: To determine the nature and extent of minor neuropsychological deficits in patients with subjective cognitive decline (SCD) and their association with cerebrospinal fluid (CSF) biomarkers of Alzheimer’s disease (AD). Method: We analyzed data from n=449 cognitively normal participants (n=209 healthy controls, n=240 SCD patients) from an interim data release of the German Center for Neurodegenerative Diseases Longitudinal Cognitive Impairment and Dementia Study (DELCODE). An extensive neuropsychological test battery was applied at baseline for which...

Supplementary mass spectonomy data to analyse generation of spliced mutant Kras/RAC2 epitopes

Gerald Willimsky
In vitro proteasome processing of neo-splicetopes does not predict their presentation in vivo Proteasome catalyzed peptide splicing (PCPS) of cancer-driving antigens could generate attractive neoepitopes to be targeted by TCR-based adoptive T cell therapy. Based on a spliced peptide prediction algorithm TCRs were generated against putative KRASG12V and RAC2P29L derived neo-splicetopes with high HLA-A*02:01 binding affinity. TCRs generated in mice with a diverse human TCR repertoire specifically recognized the respective target peptides with high efficacy....

Data from: Quantitative time-resolved analysis reveals intricate, differential regulation of standard- and immuno-proteasomes

Juliane Liepe, Hermann-Georg Holzhütter, Elena Bellavista, Peter M. Kloetzel, Michael P. H. Stumpf & Michele Mishto
Proteasomal protein degradation is a key determinant of protein half-life and hence of cellular processes ranging from basic metabolism to a host of immunological processes. Despite its importance the mechanisms regulating proteasome activity are only incompletely understood. Here we use an iterative and tightly integrated experimental and modelling approach to develop, explore and validate mechanistic models of proteasomal peptide-hydrolysis dynamics. The 20S proteasome is a dynamic enzyme and its activity varies over time because of...

Data from: Predictors of new remote cerebral microbleeds after intravenous thrombolysis for ischemic stroke

Tim Bastian Braemswig, Kersten Villringer, Guillaume Turc, Hebun Erdur, Jochen B. Fiebach, Heinrich J. Audebert, Matthias Endres, Christian H. Nolte & Jan F. Scheitz
Objective: To assess the frequency, associated factors, and underlying vasculopathy of new remote cerebral microbleeds (CMB), as well as the risk of concomitant hemorrhagic complications related to new CMBs, after intravenous thrombolysis (IVT) in acute stroke patients. Methods: We conducted an observational study using data from our local thrombolysis registry. We included consecutive stroke patients with MRI (3-Tesla)-based IVT and a follow-up MRI the next day between 2008–2017 (n=396). Only CMBs located outside of the...

Data from: Hodge decomposition of wall shear stress vector fields characterizing biological flows

Faniry H. Razafindrazaka, Pavel Yevtushenko, Konstantin Poelke, Konrad Polthier & Leonid Goubergrits
A discrete boundary-sensitive Hodge decomposition is proposed as a central tool for the analysis of wall shear stress (WSS) vector fields in aortic blood flows. The method is based on novel results for the smooth and discrete Hodge-Morrey-Friedrichs decomposition on manifolds with boundary and subdivides the WSS vector field into five components: gradient (curl-free), co-gradient (divergence-free), and three harmonic fields induced from the boundary, which are called the center, Neumann and Dirichlet fields. First, an...

A comprehensive survey among statistical members of medical ethics committees in Germany on their personal impression of completeness and correctness of biostatistical aspects of submitted study protocols

Geraldine Rauch
Original data of a comprehensive survey among statistical members of medical ethics committees in Germany on their personal impression of completeness and correctness of biostatistical aspects of submitted study protocols. To enable reproduction of the results presented in the corresponding paper, the final dataset is freely available here, without any information on the medical ethics committee’s federal state to avoid any risk of potential re-identification.

Data from: Clinical presentation and management of SMART syndrome

Sebastian F. Winter, Joshua P. Klein, Eugene J. Vaios, Philipp Karschnia, Eudocia Q. Lee, Helen A. Shih, Franziska Loebel & Jorg Dietrich
Stroke-like migraine attacks after radiation therapy (SMART) syndrome represents a rare but serious condition manifesting years after cranial radiation therapy (RT). Characterized by migraine-type headaches, stroke-like deficits and/or seizures and MR imaging abnormalities, including cortical gyriform enhancement in irradiated brain regions, SMART remains diagnostically and therapeutically challenging. Distinction from tumor progression is difficult and treatment options are limited. Although frequently reversible, SMART episodes can recur and effectuate persistent neurologic and/or imaging sequelae.

Data from: Investigating the zoonotic origin of the West African Ebola epidemic

Almudena Marí Saéz, Sabrina Weiss, Kathrin Nowak, Vincent Lapeyre, Fee Zimmermann, Ariane Düx, Hjalmar S. Kühl, Moussa Kaba, Sébastien Regnaut, Kevin Merkel, Andreas Sachse, Ursula Thiesen, Lili Villányi, Christophe Boesch, Piotr W. Dabrowski, Aleksandar Radonic, Andreas Nitsche, Siv Aina J. Leendertz, Stefan Petterson, Stephan Becker, Verena Krähling, Emmanuel Couacy-Hymann, Chantal Akoua-Koffi, Natalie Weber, Lars Schaade … & Fabian H. Leendertz
The severe Ebola virus disease epidemic occurring in West Africa stems from a single zoonotic transmission event to a 2-year-old boy in Meliandou, Guinea. We investigated the zoonotic origins of the epidemic using wildlife surveys, interviews, and molecular analyses of bat and environmental samples. We found no evidence for a concurrent outbreak in larger wildlife. Exposure to fruit bats is common in the region, but the index case may have been infected by playing in...

Data from: Patellar tendon stiffness is not reduced during pregnancy

Marie Elena Bey, Robert Marzilger, Larry Hinkson, Adamantios Arampatzis & Kirsten Legerlotz
It is believed that hormonal changes during pregnancy lead to an increased compliance in ligaments and tendons, increasing the risk to suffer from connective tissue injuries particularly during exercise. While the laxity of the pelvic ligaments may increase to facilitate childbirth, to our knowledge no study has ever investigated the mechanical properties of human tendons in different stages of pregnancy. Thus, the purpose of our longitudinal study was to investigate the mechanical properties of the...

Data from: Re-assessing the diversity of negative strand RNA viruses in insects

Simon Käfer, Sofia Paraskevopoulou, Florian Zirkel, Nicolas Wieseke, Alexander Donath, Malte Petersen, Terry C. Jones, Shanlin Liu, Xin Zhou, Martin Middendorf, Sandra Junglen, Bernhard Misof & Christian Drosten
The spectrum of viruses in insects is important for subjects as diverse as public health, veterinary medicine, food production, and biodiversity conservation. The traditional interest in vector-borne diseases of humans and livestock has drawn the attention of virus studies to hematophagous insect species. However, these represent only a tiny fraction of the broad diversity of Hexapoda, the most speciose group of animals. Here, we systematically probed the diversity of negative strand RNA viruses in the...

Data for: Effect of using a cognitive aid on content and feasibility of debriefings of simulated emergencies

Julia Freytag, Fabian Stroben, Wolf Hautz, Dorothea Penders & Juliane Kämmer
Background Adverse events in patient care are often caused by failures in teamwork. Simulation training and its debriefing can contribute to improving teamwork and thus patient care. When conducting debriefings, there are several design factors that can potentially influence learning outcomes. This study examines the use of a cognitive aid to help structure the content of debriefings and compares it with debriefings that are merely roughly structured. In addition, the feasibility of the debriefing, the...

Data from: Presynaptic GABAB receptors functionally uncouple somatostatin interneurons from the active hippocampal network

Sam A. Booker, Harumi Harada, Claudio Elguate, Julia Bank, Bartos Marlene, Akos Kulik & Imre Vida
Information processing in cortical neuronal networks relies on properly balanced excitatory and inhibitory neurotransmission. A ubiquitous motif for maintaining this balance is the somatostatin interneuron (SOM-IN) feedback microcircuit. Here, we investigate the modulation of this microcircuit by presynaptic GABAB receptors (GABABRs) in the rat hippocampus. Whole-cell recordings from SOM-INs reveal that both excitatory and inhibitory synaptic inputs are strongly inhibited by GABABRs, while optogenetic activation of the interneurons shows that their inhibitory output is also...

Unvalidated R source code

Geraldine Rauch
Univalidated R source code implmenting a performance comparison of adaptive sample size recalculation rules including in particular the new performance score by Herrmann et al. (2019)

Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages

Carolin Ulbricht, Ruth Leben, Asylkhan Rakhymzhan, Frank Kirchhoff, Lars Nitschke, Helena Radbruch, Raluca Niesner & Anja Hauser
Calcium is a universal second messenger present in all eukaryotic cells. The mobilization and storage of Ca2+ ions drives a number of signaling-related processes, stress-responses or metabolic changes, all of which are relevant for the development of immune cells and their adaption to pathogens. Here, we introduce the FRET-reporter mouse YellowCaB expressing the genetically encoded calcium indicator TN-XXL in B lymphocytes. Calcium-induced conformation change of TN-XXL results in FRET-donor quenching measurable by two-photon fluorescence lifetime...

Appendix: Association between hemostatic profile and migraine: a Mendelian randomization analysis

Yanjun Guo, Pamela Rist, Maria Sabater-Lleal, Paul De Vries, Nicholas Smith, Paul Ridker, Tobias Kurth & Daniel Chasman
Objective: To assess support for a causal relationship between hemostatic measures and migraine susceptibility using genetic instrumental analysis. Methods: Two-sample Mendelian randomization (MR) instrumental leveraging available genome-wide association study (GWAS) summary statistics was applied to hemostatic measures as potential causal for migraine and its subtypes, migraine with aura (MA) and migraine without aura (MO). Twelve blood-based measures of hemostasis were examined, including plasma level or activity of eight hemostatic factors and two fibrinopeptides together with...

Prospective Quantification of CSF Biomarkers in Antibody-Mediated Encephalitis

Gregory Day, Melanie L. Yarbrough, Peter Körtvelyessy, Harald Prüss, Robert C. Bucelli, Marvin J. Fritzler, Warren Mason, David F. Tang-Wai, Claude Steriade, Julien Hébert, Rachel L. Henson, Elizabeth M. Herries, Jack H. Ladenson, A. Sebastian Lopez-Chiriboga, Neill R. Graff-Radford, John C. Morris & Anne Fagan
Objective: To determine whether neuronal and neuroaxonal injury, neuroinflammation, and synaptic dysfunction associate with clinical course and outcomes in antibody-mediated encephalitis (AME), we measured biomarkers of these processes in CSF from patients presenting with AME and cognitively normal individuals. Methods: Biomarkers of neuronal (total tau, VILIP-1) and neuroaxonal damage (neurofilament light chain [NfL]), inflammation (YKL-40), and synaptic function (neurogranin, SNAP-25) were measured in CSF obtained from 45 patients at the time of diagnosis of NMDA...

Data from: Reliability of single-use PEEP-valves attached to self-inflating bags during manual ventilation of neonates – an in vitro study

Julia Christine Hartung, Silke Wilitzki, Marta Thio-Lluch, Arjan B. Te Pas, Gerd Schmalisch & Charles Christoph Roehr
Introduction International resuscitation guidelines suggest to use positive end-expiratory pressure (PEEP) during manual ventilation of neonates. Aim of our study was to test the reliability of self-inflating bags (SIB) with single-use PEEP valves regarding PEEP delivery and the effect of different peak inflation pressures (PIP) and ventilation rates (VR) on the delivered PEEP. Methods Ten new single-use PEEP valves from 5 manufacturers were tested by ventilating an intubated 1kg neonatal manikin containing a lung model...

Data from: Immune checkpoint inhibitor–related myositis and myocarditis in patients with cancer

Mehdi Touat, Thierry Maisonobe, Samuel Knauss, Omar Ben Hadj Salem, Baptiste Hervier, Karine Auré, Tali-Anne Szwebel, Nora Kramkimel, Claire Lethrosne, Jean-Frédéric Bruch, Pauline Laly, Jacques Cadranel, Nicolas Weiss, Anthony Béhin, Yves Allenbach, Olivier Benveniste, Timothée Lenglet, Dimitri Psimaras, Werner Stenzel & Sarah Léonard-Louis
Objective: To report the clinicopathological features and outcome of myositis in patients treated with immune checkpoint inhibitors (irMyositis). Methods: We retrospectively analyzed patients diagnosed with irMyositis in tertiary centers in Paris, France, and Berlin, Germany, from January 2015, to July 2017. Main outcomes were clinical manifestations and muscle histology, which included MHC-I, C5b-9, CD3, CD4, CD8, CD20, CD68, PD-1, PD-L1 and PD-L2 immunohistochemical stains. Results: Ten patients with metastatic cancer were included; median age was...

Data from: 7 Tesla MRI of the ex vivo human brain at 100 micron resolution

Brian L. Edlow, Azma Mareyam, Andreas Horn, Jonathan R. Polimeni, Thomas Witzel, M. Dylan Tisdall, Jean Augustinack, Jason P. Stockmann, Bram R. Diamond, Allison Stevens, Lee S. Tirrell, Rebecca D. Folkerth, Lawrence L. Wald, Bruce Fischl & Andre Van Der Kouwe
We present an ultra-high resolution MRI dataset of an ex vivo human brain specimen. The brain specimen was donated by a 58-year-old woman who had no history of neurological disease and died of non-neurological causes. After fixation in 10% formalin, the specimen was imaged on a 7 Tesla MRI scanner at 100 µm isotropic resolution using a custom-built 31-channel receive array coil. Single-echo multi-flip Fast Low-Angle SHot (FLASH) data were acquired over 100 hours of...

Endothelial and leukocyte-derived microvesicles and cardiovascular risk after stroke: PROSCIS-B

Shufan Huo, Nicolle Kränkel, Alexander H. Nave, Pia Sophie Sperber, Jessica Rohmann, Sophie K. Piper, Peter Heuschmann, Ulf Landmesser, Matthias Endres, Bob Siegerink & Thomas G. Liman
Objective: To determine the role of circulating endothelial microvesicles (EMV) and microvesicles (MV) of other origins on long-term cardiovascular outcomes after stroke, we measured them in a cohort of first-ever stroke patients and observed them for three years. Methods: In the PROSpective Cohort with Incident Stroke Berlin (PROSCIS-B), patients with first-ever ischemic stroke were followed for three years. The primary combined endpoint consisted of recurrent stroke, myocardial infarction, and all-cause mortality. Levels of EMV, leukocyte-derived...

Sex differences in response to triptans: a systematic review and meta-analysis

Daphne Van Casteren, Tobias Kurth, A.H. Jan Danser, Gisela M. Terwindt & Antoinette MaassenVanDenBrink
Objective: To examine the effect of sex on clinical response to triptans and to determine whether these differences are related to pharmacokinetics of triptans in men and women, we performed a systematic review and meta-analysis. Methods: We searched clinical trials distinguishing clinical response to or pharmacokinetic parameters of triptans between sexes in PubMed, MEDLINE, Cochrane Library, Embase and Web of Science up to Dec 12, 2019. Analysis was based on data extracted from published reports....

Registration Year

  • 2021
    7
  • 2020
    4
  • 2019
    4
  • 2018
    3
  • 2017
    1
  • 2016
    1
  • 2015
    1
  • 2014
    1

Resource Types

  • Dataset
    22

Affiliations

  • Charité
    22
  • Massachusetts General Hospital
    2
  • Humboldt University of Berlin
    2
  • Inserm
    2
  • Freie Universität Berlin
    2
  • Brigham and Women's Hospital
    2
  • Leiden University Medical Center
    2
  • Max Planck Institute for Ornithology
    1
  • New York University Langone Medical Center
    1
  • University of Pennsylvania
    1