14 Works

MiR-30a-5p frequently downregulated in prostate cancer inhibits cell proliferation via targeting PCLAF

Hu Zhao, Xiaofeng Lai, Wei Zhang, Hehuan Zhu, Shenhang Zhang, Weizhen Wu, Shuiliang Wang, Minying Tang, Zhen Deng & Jianming Tan
MicroRNAs (miRNAs) have vastly expanded our view of RNA world in intracellular signal regulating networks. Here, we functionally characterized a normally highly expressed miRNA, miR-30a-5p (MIMAT0000087), which exhibits downregulated expression profiles in prostate cancer samples. MiR-30a-5p knockdown and overexpression in PC-3 cell line alters cell proliferation supporting a tumour suppressor role. We also discovered that PCLAF is the direct target of miR-30a-5p. Notably, PC-3 cell proliferation is inhibited by miR-30a-5p/PCLAF axis. miR-30a-5p represents a novel...

Geographical and temporal variation of multiple paternity in invasive mosquitofish (Gambusia holbrooki, Gambusia affinis)

Jiancao Gao, Francesco Santi, Linjun Zhou, Xiaoqin Wang, Rüdiger Riesch & Martin Plath
Multiple paternity (MP) increases offspring’s genetic variability, which could be linked to invasive species’ evolvability in novel distribution ranges. Shifts in MP can be adaptive, with greater MP in harsher/colder environments or towards the end of the reproductive season, but climate could also affect MP indirectly via its effect on reproductive life histories. We tested these hypotheses by genotyping N = 2,903 offspring from N = 306 broods of two closely related livebearing fishes, Gambusia...

Cholesterol depletion sensitizes gallbladder cancer to cisplatin by impairing DNA damage response

Yonglong Zhang, Yanfeng Liu, Jinlin Duan, Hui Wang, Yuchen Zhang, Ke Qiao & Jian Wang
Gallbladder cancer (GBC) is the common malignancy of the bile tract system with extremely poor clinical outcomes, owing to its metastatic property and intrinsic resistance to the first-line drugs. Although it is well-established that cholesterol abnormity contributes to gallstone formation, a leading risk factor for GBC, the link of cholesterol homeostasis with GBC has not been investigated. The present study systematically examined the genes implicated in cholesterol homeostasis, and revealed altered gene expressions of de...

Data from: Correlated divergence of female and male genitalia in replicated lineages with ongoing ecological speciation

Ryan Greenway, Rachel McNemee, Alexander Okamoto, Martin Plath, Lenin Arias‐Rodriguez, Michael Tobler & Lenin Arias-Rodriguez
Divergence of genital traits among lineages has the potential to serve as a reproductive isolating barrier when copulation, insemination, or fertilization are inhibited by incompatibilities between female and male genitalia. Despite widespread evidence for genital trait diversity among closely related lineages and coevolution of female and male genitalia within lineages, few studies have investigated genital evolution during the early stages of speciation. We quantified genital variation in replicated population pairs of Poecilia mexicana with ongoing...

Data from: Nonlinearity of root trait relationships and the root economics spectrum

Deliang Kong, Junjian Wang, Huifang Wu, Oscar J. Valverde-Barrantes, Ruili Wang, Hui Zeng, Paul Kardol, Haiyan Zhang & Yulong Feng
The root economics spectrum (RES), a common hypothesis postulating a tradeoff between resource acquisition and conservation traits, is being challenged by conflicting relationships between root diameter, tissue density (RTD) and root nitrogen concentration (RN). Here, we analyze a global trait dataset of absorptive roots for over 800 plant species. For woody species (but not for non-woody species), we find nonlinear relationships between root diameter and RTD and RN, which stem from the allometric relationship between...

Expression Analysis and Functional Characterization of CER1 Family Genes Involved in Very-LongChain Alkanes Biosynthesis in Brachypodium distachyon

Li Quan & Hongqi Wu
Cuticular wax accumulation and composition affects drought resistance in plants. Brachypodium distachyon plants subjected to water deficit and polyethylene glycol treatments resulted in a significant increase in total wax load, in which very-long-chain (VLC) alkanes were more sensitive to these treatments than other wax compounds, implying that VLC alkanes biosynthesis plays a more important role in drought resistance in B. distachyon. ECERIFERUM1 (CER1) has been reported to encode a core enzyme involved in VLC alkanes...

MiR-150-5p retards the progression of myocardial fibrosis by targeting EGR1

Jie Shen, Wanhong Xing, Fangqi Gong, Wei Wang, Yufeng Yan, Yiying Zhang, Chunhong Xie & Songling Fu
To investigate the differential expression of microRNA-150-5p (miR-150-5p) and early growth response 1 (EGR1) in myocardial fibrosis (MF) cells, and determine the effect between miR-150-5p and EGR1 on MF. Human MF cells were generated via Trypanosoma cruzi (T. cruzi) infection, a mouse model of MF was generated via angiotensin II. The expression levels of miR-150-5p and EGR1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay. The correlation between miR-150-5p and...

MiR-150-5p retards the progression of myocardial fibrosis by targeting EGR1

Jie Shen, Wanhong Xing, Fangqi Gong, Wei Wang, Yufeng Yan, Yiying Zhang, Chunhong Xie & Songling Fu
To investigate the differential expression of microRNA-150-5p (miR-150-5p) and early growth response 1 (EGR1) in myocardial fibrosis (MF) cells, and determine the effect between miR-150-5p and EGR1 on MF. Human MF cells were generated via Trypanosoma cruzi (T. cruzi) infection, a mouse model of MF was generated via angiotensin II. The expression levels of miR-150-5p and EGR1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay. The correlation between miR-150-5p and...

The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment

Qin Zhou, Xiaoyu Gu, Jiachen Dong, Chao Zhu, Zhen Cai, Dongmei He, Chi Yang, Ling Xu & Jiawei Zheng
The repair of periodontal bone tissue defects in patients with periodontitis is one of the major challenges for dentists. Stem cell-based bone regeneration has been considered as a promising strategy to restore the lost periodontal bone tissue. However, the local inflammatory environment of periodontal tissue affects stem cell-based periodontal bone regeneration. Toll-like receptor 2 (TLR2), a member of the TLR family, plays an important role in regulating immunoreaction. Previous studies have shown that the activation...

The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment

Qin Zhou, Xiaoyu Gu, Jiachen Dong, Chao Zhu, Zhen Cai, Dongmei He, Chi Yang, Ling Xu & Jiawei Zheng
The repair of periodontal bone tissue defects in patients with periodontitis is one of the major challenges for dentists. Stem cell-based bone regeneration has been considered as a promising strategy to restore the lost periodontal bone tissue. However, the local inflammatory environment of periodontal tissue affects stem cell-based periodontal bone regeneration. Toll-like receptor 2 (TLR2), a member of the TLR family, plays an important role in regulating immunoreaction. Previous studies have shown that the activation...

Circular RNA ZFR accelerates non-small cell lung cancer progression by acting as a miR-101-3p sponge to enhance CUL4B expression

Haifeng Zhang, Xiaolong Wang, Baoli Hu, Feng Zhang, Haitao Wei & Li Li
Non-small-cell lung carcinoma (NSCLC) is the most common type of lung cancer. Circular RNA ZFR (circZFR) is an identified circular RNA (circRNA) that is correlated with cancer progression. However, the role of circZFR in NSCLC remains unknown. In the present study, we aimed to investigate the function of circZFR in NSCLC and the underlying mechanism. The expression patterns of circZFR were determined using qRT-PCR in NSCLC samples and cell lines. The subcellular distribution of circZFR...

Circular RNA ZFR accelerates non-small cell lung cancer progression by acting as a miR-101-3p sponge to enhance CUL4B expression

Haifeng Zhang, Xiaolong Wang, Baoli Hu, Feng Zhang, Haitao Wei & Li Li
Non-small-cell lung carcinoma (NSCLC) is the most common type of lung cancer. Circular RNA ZFR (circZFR) is an identified circular RNA (circRNA) that is correlated with cancer progression. However, the role of circZFR in NSCLC remains unknown. In the present study, we aimed to investigate the function of circZFR in NSCLC and the underlying mechanism. The expression patterns of circZFR were determined using qRT-PCR in NSCLC samples and cell lines. The subcellular distribution of circZFR...

MiR-30a-5p frequently downregulated in prostate cancer inhibits cell proliferation via targeting PCLAF

Hu Zhao, Wei Zhang, Xiaofeng Lai, Hehuan Zhu, Shenhang Zhang, Weizhen Wu, Shuiliang Wang, Minying Tang, Zhen Deng & Jianming Tan
MicroRNAs (miRNAs) have vastly expanded our view of RNA world in intracellular signal regulating networks. Here, we functionally characterized a normally highly expressed miRNA, miR-30a-5p (MIMAT0000087), which exhibits downregulated expression profiles in prostate cancer samples. MiR-30a-5p knockdown and overexpression in PC-3 cell line alters cell proliferation supporting a tumour suppressor role. We also discovered that PCLAF is the direct target of miR-30a-5p. Notably, PC-3 cell proliferation is inhibited by miR-30a-5p/PCLAF axis. miR-30a-5p represents a novel...

MiR-30a-5p frequently downregulated in prostate cancer inhibits cell proliferation via targeting PCLAF

Hu Zhao, Wei Zhang, Xiaofeng Lai, Hehuan Zhu, Shenhang Zhang, Weizhen Wu, Shuiliang Wang, Minying Tang, Zhen Deng & Jianming Tan
MicroRNAs (miRNAs) have vastly expanded our view of RNA world in intracellular signal regulating networks. Here, we functionally characterized a normally highly expressed miRNA, miR-30a-5p (MIMAT0000087), which exhibits downregulated expression profiles in prostate cancer samples. MiR-30a-5p knockdown and overexpression in PC-3 cell line alters cell proliferation supporting a tumour suppressor role. We also discovered that PCLAF is the direct target of miR-30a-5p. Notably, PC-3 cell proliferation is inhibited by miR-30a-5p/PCLAF axis. miR-30a-5p represents a novel...

Registration Year

  • 2019
    14

Resource Types

  • Dataset
    14

Affiliations

  • North West Agriculture and Forestry University
    14
  • Zhejiang University
    10
  • Central South University
    10
  • Capital Medical University
    10
  • Sun Yat-sen University
    8
  • Fudan University
    8
  • Air Force Medical University
    6
  • Fudan University Shanghai Cancer Center
    6
  • Huazhong University of Science and Technology
    6
  • Nanjing University
    6