Additional file 3: Figure S1. Cell proliferation assay results. Cell proliferation assay results of Raw264.7 cell lines treated with OH2 MOI=1 (red), OH2 MOI=0.5 (blue) and untreated (black) group by CCK8 assay in 72 hours. Figure S2. OH2 lysates induce RAW264.7 polarization and phagocytosis in vitro. A. Demonstration of the analysis of the phagocytosis by flow cytometry. B. Cell proliferation assay results of CT26, MC38 and 4T-1 cell lines treated with lysate (red), CFS (blue),...

This document describes the basic drawing data of Figure 6

Di-2-ethylhexyl phthalate (DEHP), a known persistent organic pollutant, can increase the sperm DNA fragmentation index (DFI). To investigate the mechanism underlying the repair of DEHP-induced sperm DNA damage in mice by Wuwei Fuzheng Yijing (WFY) formula. The potential targets of WFY and sperm DNA fragment (SDF) were obtained from the TCMSP, BATMAN-TCM, OMIM and GeneCards. The protein–protein interaction (PPI) network, GO and KEGG pathway analyses of WFY-SDF were constructed. An animal model of DEHP-induced sperm...

Plant seed germination is easily influenced by endogenous gene expression, hormones and environmental conditions. Here, we investigated a Populus R2R3-MYB transcription factor (referred to as MYB94) that plays a role in seed germination in close relation to the hormone abscisic acid (ABA). Two independent Arabidopsis transgenic lines overexpressing MYB94 (Oe-lines) and wild-type (Wt) were no clear differences in the seed germination rates under normal conditions, but the germination rates of the two Oe-lines were both...

Macroautophagy/autophagy is a conserved cellular mechanism to degrade unneeded cytoplasmic proteins and organelles to recycle their components, and it is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Whereas autophagy is essential for early development of embryos, no information exists regarding its functions during the transition from naive-to-primed pluripotency. Here, by using an in vitro transition model of ESCs to epiblast-like cells (EpiLCs), we find that dynamic changes in ATG7-dependent autophagy are...

The remnant Comei igneous province in SE Tibet has been recognized as a product of early Kerguelen mantle plume activity. In this paper, we investigate mafic dykes and sills intruded into Lakang Formation in the southeastern Tethyan Himalaya. Based on new whole-rock geochemical, Sr-Nd-Pb isotopic and SHRIMP zircon U-Pb geochronological data, these rocks can be divided into three groups. Group 1 is composed of OIB-like mafic dykes and sills with lower εNd(t) (+1.78-+2.55) and dated...

The launch of the selection process for National Development Zones (NDZs) marked a fundamental change in the construction of development zones, making it an essential position for local authorities to implement high-quality development. Based on the data of prefecture-level cities in China from 2000 to 2018, this paper examines the impact and mechanism of selecting NDZs on urban green innovation through a double-difference spatial durbin model using the selection of NDZs as a “quasi-natural experiment”....

Additional file 5: Table S4. The results of univariate Cox proportional hazards regression analyses between clinical features and risk score in the training, testing, and entire sets.

Additional file 4: Table S2. List of 2,443 leaf-specific genes.

Additional file 5: Table S3. List of 2,061 up-regulated genes (‘sativa’ Vs ‘falcata’).

Additional file 3: Supplementary Table 2. Correlation statistics between CPSF3 and immune checkpoint-related genes.

Additional file 1: Fig. S1. Partial correlation analysis between BMI and bilateral insula in SZ group, controlling for sex, age and education. Fig. S2. Partial correction analysis between negative symptom and bilateral insula in SZ group, controlling for sex, age and education. Fig. S3. Results of bilateral insula in the whole-brain VBM analyses. Red indicates the bilateral insula in the main effects of diagnosis (SZ

Additional file 1: Table S1. Gene lis for heatmap.

This document describes the basic drawing data of Figure 3 (a)

This document describes the data of each point in the A1B3C3D2E1 structure irradiance distribution map

Nucleomodulins are secreted bacterial proteins whose molecular targets are located in host cell nuclei. They are gaining attention as critical virulence factors that either modify the epigenetics of host cells or directly regulate host gene expression. Mycoplasma bovis is a major veterinary pathogen that secretes several potential virulence factors. The aim of this study was to determine whether any of their secreted proteins might function as nucleomodulins. After an initial in silico screening, the nuclear...

Additional file 7.

Additional file 8.

Supplementary Material 1

Additional file 1.

Additional file 11.

Additional file 4.

Additional file 1. 1229 lncRNAs screens associated with immune genes.

Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centres. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH)....

Extraintestinal pathogenic Escherichia coli (ExPEC) is an important zoonotic pathogen. Recently, ExPEC has been reported to be an emerging problem in pig farming. However, the mechanism of pathogenicity of porcine ExPEC remains to be revealed. In this study, we constructed a transposon (Tn) mutagenesis library covering Tn insertion in over 72% of the chromosome-encoded genes of a virulent and multi-drug resistant porcine ExPEC strain PCN033. By using a mouse infection model, a transposon-directed insertion site...