5 Works

Systematic characterization of wing mechanosensors that monitor airflow and wing deformations

Joseph Fabian, Igor Siwanowicz, Myriam Uhrhan, Masateru Maeda, Richard Bomphrey & Huai-Ti Lin
Animal wings deform during flight in ways that can enhance lift, facilitate flight control, and mitigate damage. Monitoring the structural and aerodynamic state of the wing is challenging because deformations are passive, and the flow fields are unsteady; it requires distributed mechanosensors that respond to local airflow and strain on the wing. Without a complete map of the sensor arrays, it is impossible to model control strategies underpinned by them. Here, we present the first...

Genomic changes underlying repeated niche shifts in an adaptive radiation

David A. Marques, Felicity C. Jones, Federica Di Palma, David M. Kingsley & Thomas E. Reimchen
In adaptive radiations, single lineages rapidly diversify by adapting to many new niches. Little is known yet about the genomic mechanisms involved, i.e. the source of genetic variation or genomic architecture facilitating or constraining adaptive radiation. Here, we investigate genomic changes associated with repeated invasion of many different freshwater niches by threespine stickleback in the Haida Gwaii archipelago, Canada, by re-sequencing single genomes from one marine and 28 freshwater populations. We find 89 likely targets...

Ground truth data used to train the synapse classifier used in Lillvis et al., 2022 for ExLLSM circuit reconstruction

Joshua Lillvis
Brain function is mediated by the physiological coordination of a vast, intricately connected network of molecular and cellular components. The physiological properties of network components can be quantified with high throughput; the ability to assess many animals per study has been key to relating physiological properties to behavior. Conversely, detailed anatomical properties (e.g., the synaptic connectivity of molecularly-defined cell types across an entire circuit) are presently quantifiable only with low throughput; thus we know very...

Conserved structural elements specialize ATAD1 as a membrane protein extraction machine

Lan Wang, Hannah Toutkoushian, Vladislav Belyy, Claire Kokontis & Peter Walter
The mitochondrial AAA protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria’s protein import capacity. In doing so, it helps to maintain proteostasis in mitochondria. How ATAD1 tackles the energetic challenge to extract hydrophobic membrane proteins from the lipid bilayer and what structural features adapt ATAD1 for its particular function has remained...

Conservation and divergence of cortical cell organization in human and mouse revealed by MERFISH

Rongxin Fang
Please contact Rongxin Fang (r3fang@fas.harvard.edu) for any question about the dataset. The human cerebral cortex has tremendous cellular diversity. How different cell types are organized in the human cortex and how cellular organization varies across species remain unclear. In this study, we performed spatially resolved single-cell profiling of 4000 genes using multiplexed error-robust fluorescence in situ hybridization (MERFISH), identified more than 100 transcriptionally distinct cell populations, and generated a molecularly defined and spatially resolved cell...

Registration Year

  • 2022

Resource Types

  • Dataset


  • Howard Hughes Medical Institute
  • University of Adelaide
  • University of California San Francisco Medical Center
  • Royal Veterinary College
  • University of East Anglia
  • Natural History Museum of Basel
  • Imperial College London
  • University of California San Francisco
  • University of Victoria
  • Friedrich Miescher Laboratory