In the present study, a total of 7793 samples from 5 different types of hosts were collected and tested, with a seroprevalence of 2.4% (184/7793). Although the seroprevalence of human and animal brucellosis is relatively low, numbers of human brucellosis cases reported have increased continuously from 2004 to 2018. A total of 118 Brucella strains containing 4 biotypes were obtained, including Brucella melitensis bv.1 (n = 8) and bv.3 (n = 106), Brucella abortus bv.3...

To determine whether zinc finger protein 521 (Zfp521) has a chondroprotective effect by maintaining extracellular matrix (ECM) homeostasis to attenuate osteoarthritis (OA). In chondrocytes, Zfp521 was overexpressed or silenced to detect its effects on proliferation, apoptosis, and ECM homeostasis. Adenovirus encoding Zfp521 was injected into the knee joints of anterior cruciate ligament transection rats to test its efficacy against OA. Combined with proteomic analysis, the molecular mechanism of Zfp521 in cartilage degeneration was further explored....

Supplemental material, sj-xlsx-2-tct-10.1177_15330338221145994 for Multi-omics Analysis of the Role of PHGDH in Colon Cancer by Zhihui Dai, Lin Chen, KaiLing Pan, XiaoYa Zhao, WenXia Xu, JinLin Du and Chungen Xing in Technology in Cancer Research & Treatment

A material-structure integrated design method is proposed in this paper, with which micropillar and microwedge arrayed surfaces are fabricated based on a novel nanoparticle-reinforced silicone rubber composite (NRSRC) with high mechanical strength and strong surface adhesion. It is found that the micropillar-arrayed surface and the microwedge-arrayed surface show a normal adhesive strength of 50.9 kPa and a shear adhesive strength of 137.3 kPa, respectively, which are much higher than those of previously reported adhesive surfaces...

A material-structure integrated design method is proposed in this paper, with which micropillar and microwedge arrayed surfaces are fabricated based on a novel nanoparticle-reinforced silicone rubber composite (NRSRC) with high mechanical strength and strong surface adhesion. It is found that the micropillar-arrayed surface and the microwedge-arrayed surface show a normal adhesive strength of 50.9 kPa and a shear adhesive strength of 137.3 kPa, respectively, which are much higher than those of previously reported adhesive surfaces...

FISH figures, calls records and figures and whole-genome NJ tree

NASA is currently planning return missions to the Moon for further exploration and research. The Moon is covered by a layer of potentially reactive fine dust, which could pose a toxicological risk of exposure to explorers. To assess this risk, we exposed rats to lunar dust (LD) that was collected during the Apollo14 mission. Rats were exposed to respirable sizes of LD at concentrations of 0, 2.1, 6.8, 20.8, or 60.6 mg/m3 for 4 weeks....

Our study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expression of WTAP, FLNA and autophagy-related proteins in cells. Our results confirmed the up-regulation of WTAP in colon cancer and its promoting effect on proliferation and inhibiting effect on apoptosis. FLNA was the downstream gene of...

Decreased dietary protein intake (DPI) may lead to protein-energy malnutrition and may be associated with increased mortality risk. We hypothesized that longitudinal changes in dietary protein intake have independent associations with survival in peritoneal dialysis (PD) patients. 668 stable PD patients were selected in the study from January 2006 to January 2018 and were followed up until December 2019. Their three-day dietary records were collected at the baseline (the sixth month after PD) and thereafter...

This paper deals with the study of the chemical composition of cow dung and the paper-making from cow dung. With the insight into such data, we considered the effect of cooking temperatures and the potassium hydroxide concentration on paper performance. The relationship between cow dung fiber, such as morphology, chemical composition, crystallization, and chemical state structure, and paper performance were studied. Meanwhile, the mechanical performance of cow dung paper and the degradation of cow dung...

This paper deals with the study of the chemical composition of cow dung and the paper-making from cow dung. With the insight into such data, we considered the effect of cooking temperatures and the potassium hydroxide concentration on paper performance. The relationship between cow dung fiber, such as morphology, chemical composition, crystallization, and chemical state structure, and paper performance were studied. Meanwhile, the mechanical performance of cow dung paper and the degradation of cow dung...

Abstract Compared to other orthodontic acceleration methods such as drug, electric current, and laser, vibratory loading is less invasive and easier to use. But the optimal duration for vibratory load application has not been determined, nor can the alveolar bone parameters be predicted after vibratory load application. Therefore, this work examined the mechanism of vibration-accelerated alveolar bone reconstruction and established a numerical model for simulating alveolar bone damage caused by vibration loads. That is, the...

5-Methylcytosine (m5C) is a widespread post-transcriptional RNA modification and is reported to be involved in manifold cellular responses and biological processes through regulating RNA metabolism. However, its regulatory role in antiviral innate immunity has not yet been elucidated. Here, we report that NSUN2, a typical m5C methyltransferase, negatively regulates type I interferon responses during various viral infections, including SARS-CoV-2. NSUN2 specifically mediates m5C methylation of IRF3 mRNA and accelerates its degradation, resulting in low levels...

Abstract Compared to other orthodontic acceleration methods such as drug, electric current, and laser, vibratory loading is less invasive and easier to use. But the optimal duration for vibratory load application has not been determined, nor can the alveolar bone parameters be predicted after vibratory load application. Therefore, this work examined the mechanism of vibration-accelerated alveolar bone reconstruction and established a numerical model for simulating alveolar bone damage caused by vibration loads. That is, the...

Tumor-infiltrating lymphocytes (TILs) are vital elements of the tumor microenvironment (TME), and the anti-tumor activity of TILs on colorectal cancer (CRC) has been a topic of concern. However, the characteristics and prognosis of the various types of lymphocyte infiltration in CRC have not been fully explained. Our study aimed to identify distinct features and prognosis of TILs. We integrated multiple-cohort databases to illustrate the features, proportions, and prognosis of TILs on CRC. We found that...

It has been well-documented that posttraumatic stress symptoms cause impairments in health-related quality of life (HRQoL). Until now we have little data on how DSM-5 PTSD symptom dimensions relate to different aspects of HRQoL. Clarifying this question would be informative to improve the quality of life of PTSD patients. This study aimed to investigate the effects of dimensions of a well-supported seven-factor model of DSM-5 PTSD symptoms on physical and psychosocial HRQoL. A total of...

ABSTRACT Soil moisture (SM) plays an important role in regulating the global water cycle, especially in arid areas, and is one of the main indicators of ecological environmental health. Although traditional methods can accurately measure SM at a single sample site, they are limited in large-scale and dynamic SM monitoring. Therefore, we used the Landsat images as the data source and the soil adjusted vegetation index (SAVI) to build the adjusted SAVI (aSAVI) index by...

Supplementary files for article Synthesis and characterization of Craig-type antiaromatic species with [4n + 2] π electrons
Abstract Antiaromaticity is extended from aromaticity as a complement to describe the unsaturated cyclic molecules with antiaromatic destabilization. To prepare antiaromatic species is a particularly challenging goal in synthetic chemistry because of the thermodynamic instability of such molecules. Among that, both Hückel and Möbius antiaromatic species have been reported, whereas the Craig one has not been realized...

Our study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expression of WTAP, FLNA and autophagy-related proteins in cells. Our results confirmed the up-regulation of WTAP in colon cancer and its promoting effect on proliferation and inhibiting effect on apoptosis. FLNA was the downstream gene of...

Decreased dietary protein intake (DPI) may lead to protein-energy malnutrition and may be associated with increased mortality risk. We hypothesized that longitudinal changes in dietary protein intake have independent associations with survival in peritoneal dialysis (PD) patients. 668 stable PD patients were selected in the study from January 2006 to January 2018 and were followed up until December 2019. Their three-day dietary records were collected at the baseline (the sixth month after PD) and thereafter...

Cathepsin L (CTSL) is a promising therapeutic target for metabolic disorders and COVID-19. However, there are still no clinically available CTSL inhibitors. Our objective is to develop an approach for the discovery of potential reversible covalent CTSL inhibitors. The authors combined Chemprop, a deep learning-based strategy, and the Schrödinger CovDock algorithm to identify potential CTSL inhibitors. First, they used Chemprop to train a deep learning model capable of predicting whether a molecule would inhibit the...

The ocular surface and lacrimal gland have a frontline position in mucosal immunology. However, there have been few updates to the immune cell atlas of these tissues in recent years. To map the immune cells in murine ocular surface tissues and lacrimal gland. Central and peripheral corneas, conjunctiva, and lacrimal gland were dissociated into single cell suspensions, followed by flow cytometry. Discrepancy of immune cells between the central and peripheral corneas was compared. In the...

Supplementary file for article Synthesis and characterization of Craig-type antiaromatic species with [4n + 2] π electrons
Abstract Antiaromaticity is extended from aromaticity as a complement to describe the unsaturated cyclic molecules with antiaromatic destabilization. To prepare antiaromatic species is a particularly challenging goal in synthetic chemistry because of the thermodynamic instability of such molecules. Among that, both Hückel and Möbius antiaromatic species have been reported, whereas the Craig one has not been realized...

It has been well-documented that posttraumatic stress symptoms cause impairments in health-related quality of life (HRQoL). Until now we have little data on how DSM-5 PTSD symptom dimensions relate to different aspects of HRQoL. Clarifying this question would be informative to improve the quality of life of PTSD patients. This study aimed to investigate the effects of dimensions of a well-supported seven-factor model of DSM-5 PTSD symptoms on physical and psychosocial HRQoL. A total of...

Objective: The pathogenesis of renal osteopathy and cardiovascular disease suggests the disordered bone–vessel axis in chronic kidney disease–mineral bone disorder (CKD–MBD). However, the mechanism of the bone–vessel axis in CKD–MBD remains unclear. Methods: We established a CKD–MBD rat model to observe the pathophysiological phenotype of the bone–vessel axis and performed RNA sequencing of aortas to identify novel targets of the bone–vessel axis in CKD–MBD. Results: The microarchitecture of the femoral trabecular bone deteriorated and alveolar...