Injection of YiQiFuMai (YQFM) powder, a modern Chinese plant-derived medical preparation, has a therapeutic effect in heart failure (HF). However, its therapeutic mechanism remains largely unknown. To investigate the molecular mechanisms of YQFM in HF. Kinase inhibition profiling assays with 2 mg/mL YQFM were performed against a series of 408 kinases. In addition, the effects of kinase inhibition were validated in cardiomyocyte cell line H9c2. In vivo, HF with reduced ejection fraction (HFrEF) was induced...

This study was designed to explore the relationship between the deterioration of sea cucumbers and intestinal flora’s diversity change during preservation using 16S rRNA gene sequencing. We show that the body wall of sea cucumber develops white spots on day 4, and the epidermis dissolves on day 7. The distinctive OTUs remain relatively low during four days of storage but increase on day 7. The potential gut microbiota biomarkers are Psychrobacter-cibarius, Ralstonia-pickettii, and Clostridiales during...

In our study titled "Retinoids inhibit ferroptosis through their radical-trapping properties and RAR-SCD-mediated monounsaturated fatty acids generation", we compared the gene expression pattern (Figure 4) and abundance of lipids (Figure 5 and S5) between Cas-NC and RETSAT-KO A549 cells treated with DMSO, IKE, or RSL3 using RNA-Seq and pseudotargeted lipidomic analysis respectively.

Additional file 3: Table S3. Codon-based tests for selection on Pvs230 in China–Myanmar border and Myanmar isolates.

Additional file 1: Table S1. Phenotypic performance and heterosis of traits in Q9311B/WSSM RILs and backcross F1s (Q9311A × RIL).

Additional file 6: Table S3. Summary of 639 main-effect QTL affecting 12 traits identified in the combined population across three environments by inclusive composite interval mapping.

Additional file 6: Table S3. Summary of 639 main-effect QTL affecting 12 traits identified in the combined population across three environments by inclusive composite interval mapping.

Additional file 8: Table S5. Classification of 2,736 epistatic QTL pairs into 13 types according to their gene actions identified in the Q9311/WSSM combined population.

Additional file 2. Table S2: Adjuvant treatment information.

To maximize survival, prey often integrates multiple anti-predator defenses. How the defenses interact to reduce predation risk is, however, poorly known. We used the rotifer Brachionus calyciflorus to investigate how morphological (spines) and behavioral (floating) defenses are integrated against a common predatory rotifer, Asplanchna brightwellii, and if their combined use improves survival. To this end, we assessed the cost of the behavioral defense and the efficiency of both defenses, individually and combined, as well as...

Additional file 1: Table S1. Prier used in this study. Table S2. List of 392 Glycine soja accessions and acumulated temperature zones. Table S3. 92 SNPs that significantly associated with flowering time in average across the two-year experiment (2020 and 2021). Table S4. The genes conresponding to the genomic regions surrounding ~50Kb of 92 SNPs that signficantly associated with flowering time in average in 2020 and 2021. Table S5. 28 candidate genes related to flowering...

Supplemental material, sj-xlsx-2-cix-10.1177_11769351221136056 for Optimizing Drug Response Study Design in Patient-Derived Tumor Xenografts by Jessica Weiss, Nhu-An Pham, Melania Pintilie, Ming Li, Geoffrey Liu, Frances A Shepherd, Ming-Sound Tsao and Wei Xu in Cancer Informatics

1. Population regulation is a central concept in ecology, yet in many cases its presence and the underlying mechanisms are difficult to demonstrate. The current paradigm maintains that marine fish populations are predominantly regulated by density-dependent recruitment. 2. While it is known that density-dependent somatic growth can be present too, its general importance is unknown and most practical applications neglect it. This study aimed to close this gap by for the first time quantifying and...

Melanoma is considered as the most frequent primary malignancy occurring in skin. Accumulating studies have suggested that long non-coding RNAs (lncRNAs) play critical parts in multiple cancers. In this study, we explored the molecular mechanism of ZBED3 antisense RNA 1 (ZBED3-AS1) in melanoma. We observed that ZBED3-AS1 expression was remarkably up-regulated in melanoma tissues, and high ZBED3-AS1 level was linked to unsatisfactory survival of melanoma patients. Then, we discovered that ZBED3-AS1 was overexpressed in melanoma...

The paternity test is a genetic test that analyzes genetic characteristics (mostly molecular markers) to identify whether two individuals have a parent-child relationship. It is frequently employed in judicial identification and economic species breeding. Because a single marker's discriminability is restricted, numerous markers are typically utilized to produce an accurate result. Obviously, having an excessive number of redundant markers wastes time and resources, and adequate approaches are required to screen reduced and efficient paternity marker...

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids...

Primary ovarian insufficiency (POI), also known as premature ovarian failure, is an ovarian defect in humans characterized by the premature depletion of ovarian follicles before the age of 40. However, the mechanisms underlying POI remain largely unknown. Here, we show that knockout of Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results in subfertility in female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed that the knockout of Epg5 affected the...

Background: Methamphetamine use is associated with several negative consequences, including neurotoxicity and greater probability of exhibiting a substance use disorder. Sigma1 receptor is involved in the neurobiological basis of several drug use disorders. Cannabidiol has received attention in the treatment of drug use disorders and neurotoxicity. Objectives: To investigate the effects of cannabidiol on methamphetamine-induced conditioned place preference (CPP) and the viability of PC12 cells. Methods: Adult male rats (n = 70) underwent methamphetamine (2...

Additional file 13: Table S8. The list of differentially methylated haplotype blocks (dMHBs) and functional enrichment for hyper dMHBs.

Additional file 14: Table S9. The list of differentially expressed (DE) genes and their functional enrichment.

Additional file 7: Table S2. Read mapping statistics of whole-genome bisulfite sequencing data and RNA sequencing data of six cows.

Additional file 4: Supplemental Table 3. Protein Ontology representation of SARS-CoV-2 proteins. Comparative information in RefSeq and UniProtKB is also provided.

Active and passive continental rifts are generally attributed to plume and subduction mechanisms, respectively. However, controversy surrounding the role of the two mechanisms continues, especially for the mid-Neoproterozoic rifts in the South China Craton. Here, we use geochemical and zircon Hf isotope data from the craton’s rift successions to examine the mechanism of rifting. The compiled geochemical data support the existence of subduction-related magmatic belts along the Jiangnan Orogen and western Yangtze margin of the...

Presbycusis is the cumulative effect of aging on hearing. Recent studies have shown that common mitochondrial gene deletions are closely related to deafness caused by degenerative changes in the auditory system, and some of these nuclear factors are proposed to participate in the regulation of mitochondrial function. However, the detailed mechanisms involved in age-related degeneration of the auditory systems have not yet been fully elucidated. In this study, we found that FOXG1 plays an important...

Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect LINC02570 expression in nasopharyngeal cell lines, NPC tissues, and chronic rhinitis tissues. Subcellular LINC02570 localization was confirmed by fluorescence in situ hybridization (FISH). The effects of LINC02570 stable knockdown and overexpression on viabillity, proliferation, migration,...