5-Methylcytosine (m5C) is a widespread post-transcriptional RNA modification and is reported to be involved in manifold cellular responses and biological processes through regulating RNA metabolism. However, its regulatory role in antiviral innate immunity has not yet been elucidated. Here, we report that NSUN2, a typical m5C methyltransferase, negatively regulates type I interferon responses during various viral infections, including SARS-CoV-2. NSUN2 specifically mediates m5C methylation of IRF3 mRNA and accelerates its degradation, resulting in low levels...

The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and...

Decreased dietary protein intake (DPI) may lead to protein-energy malnutrition and may be associated with increased mortality risk. We hypothesized that longitudinal changes in dietary protein intake have independent associations with survival in peritoneal dialysis (PD) patients. 668 stable PD patients were selected in the study from January 2006 to January 2018 and were followed up until December 2019. Their three-day dietary records were collected at the baseline (the sixth month after PD) and thereafter...

The ocular surface and lacrimal gland have a frontline position in mucosal immunology. However, there have been few updates to the immune cell atlas of these tissues in recent years. To map the immune cells in murine ocular surface tissues and lacrimal gland. Central and peripheral corneas, conjunctiva, and lacrimal gland were dissociated into single cell suspensions, followed by flow cytometry. Discrepancy of immune cells between the central and peripheral corneas was compared. In the...

This study was designed to explore the relationship between the deterioration of sea cucumbers and intestinal flora’s diversity change during preservation using 16S rRNA gene sequencing. We show that the body wall of sea cucumber develops white spots on day 4, and the epidermis dissolves on day 7. The distinctive OTUs remain relatively low during four days of storage but increase on day 7. The potential gut microbiota biomarkers are Psychrobacter-cibarius, Ralstonia-pickettii, and Clostridiales during...

Active and passive continental rifts are generally attributed to plume and subduction mechanisms, respectively. However, controversy surrounding the role of the two mechanisms continues, especially for the mid-Neoproterozoic rifts in the South China Craton. Here, we use geochemical and zircon Hf isotope data from the craton’s rift successions to examine the mechanism of rifting. The compiled geochemical data support the existence of subduction-related magmatic belts along the Jiangnan Orogen and western Yangtze margin of the...

ABSTRACT To improve the complex process of judging the degree of soil fragmentation during potato-soil separation and to establish a fast and effective judging method. Soil drop experiments were performed using sandy soil from the potato growing region of Hohhot, Inner Mongolia Autonomous Region, China. The effects of soil moisture content, hardness, volume, drop height, inclination of the separating sieve and composition of the separating sieve rod on soil fragmentation were investigated. The fractal dimension...

Developing a confidence interval for the ratio of two quantities is an important task in statistics because of its omnipresence in real world applications. For such a problem, the MOVER-R (method of variance recovery for the ratio) technique, which is based on the recovery of variance estimates from confidence limits of the numerator and the denominator separately, was proposed as a useful and efficient approach. However, this method implicitly assumes that the confidence interval for...

Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Decreased dietary protein intake (DPI) may lead to protein-energy malnutrition and may be associated with increased mortality risk. We hypothesized that longitudinal changes in dietary protein intake have independent associations with survival in peritoneal dialysis (PD) patients. 668 stable PD patients were selected in the study from January 2006 to January 2018 and were followed up until December 2019. Their three-day dietary records were collected at the baseline (the sixth month after PD) and thereafter...

ABSTRACT To improve the complex process of judging the degree of soil fragmentation during potato-soil separation and to establish a fast and effective judging method. Soil drop experiments were performed using sandy soil from the potato growing region of Hohhot, Inner Mongolia Autonomous Region, China. The effects of soil moisture content, hardness, volume, drop height, inclination of the separating sieve and composition of the separating sieve rod on soil fragmentation were investigated. The fractal dimension...

https://www.tandfonline.com/doi/full/10.1080/13543784.2023.2178894

https://www.tandfonline.com/doi/full/10.1080/13543784.2023.2178894

Two hypogean species of Triplophysa from the River Yangtze drainage, T. wudangensis and T. qingzhenensis were described in Journal of vertebrate biology. This is the supplimentary data for the two species.

Mitophagy is a form of autophagy that plays a key role in maintaining the homeostasis of functional mitochondria in the cell. Viruses have evolved various strategies to manipulate mitophagy to escape host immune responses and promote virus replication. In this study, the nucleoprotein (NP) of H1N1 virus (PR8 strain) was identified as a regulator of mitophagy. We revealed that NP-mediated mitophagy leads to the degradation of the mitochondria-anchored protein MAVS, thereby blocking MAVS-mediated antiviral signaling...

Mitophagy is a form of autophagy that plays a key role in maintaining the homeostasis of functional mitochondria in the cell. Viruses have evolved various strategies to manipulate mitophagy to escape host immune responses and promote virus replication. In this study, the nucleoprotein (NP) of H1N1 virus (PR8 strain) was identified as a regulator of mitophagy. We revealed that NP-mediated mitophagy leads to the degradation of the mitochondria-anchored protein MAVS, thereby blocking MAVS-mediated antiviral signaling...

Aim: Disentangling how stochastic and deterministic processes contribute to variation in beta diversity is a common goal for ecologists and biogeographers. However, such studies are scarce in alpine streams, especially when different diversity facets are considered. Here, we combined different approaches to examine the drivers of taxonomic, phylogenetic and functional beta diversities and discussed how our results can inform community assembly and biodiversity conservation in Tibetan streams. Location: Tibet Plateau Taxon: Macroinvertebrates Methods: We first...

Autophagy is an important protein degradation pathway in eukaryotes and is involved in multiple processes. To date, many autophagy-related genes had been studied in various plants, but a few studies have been reported on the role of autophagy in seed development. For centuries, seedlessness has been one of the most valuable characteristics of grapes. In this study, we identified a total of 33 ATG genes in the grape. Tissue-specific expression analysis showed that VvATGs expressed...

Autophagy is an important protein degradation pathway in eukaryotes and is involved in multiple processes. To date, many autophagy-related genes had been studied in various plants, but a few studies have been reported on the role of autophagy in seed development. For centuries, seedlessness has been one of the most valuable characteristics of grapes. In this study, we identified a total of 33 ATG genes in the grape. Tissue-specific expression analysis showed that VvATGs expressed...

This study was designed to explore the relationship between the deterioration of sea cucumbers and intestinal flora’s diversity change during preservation using 16S rRNA gene sequencing. We show that the body wall of sea cucumber develops white spots on day 4, and the epidermis dissolves on day 7. The distinctive OTUs remain relatively low during four days of storage but increase on day 7. The potential gut microbiota biomarkers are Psychrobacter-cibarius, Ralstonia-pickettii, and Clostridiales during...

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids...

Developing a confidence interval for the ratio of two quantities is an important task in statistics because of its omnipresence in real world applications. For such a problem, the MOVER-R (method of variance recovery for the ratio) technique, which is based on the recovery of variance estimates from confidence limits of the numerator and the denominator separately, was proposed as a useful and efficient approach. However, this method implicitly assumes that the confidence interval for...

5-Methylcytosine (m5C) is a widespread post-transcriptional RNA modification and is reported to be involved in manifold cellular responses and biological processes through regulating RNA metabolism. However, its regulatory role in antiviral innate immunity has not yet been elucidated. Here, we report that NSUN2, a typical m5C methyltransferase, negatively regulates type I interferon responses during various viral infections, including SARS-CoV-2. NSUN2 specifically mediates m5C methylation of IRF3 mRNA and accelerates its degradation, resulting in low levels...

Active and passive continental rifts are generally attributed to plume and subduction mechanisms, respectively. However, controversy surrounding the role of the two mechanisms continues, especially for the mid-Neoproterozoic rifts in the South China Craton. Here, we use geochemical and zircon Hf isotope data from the craton’s rift successions to examine the mechanism of rifting. The compiled geochemical data support the existence of subduction-related magmatic belts along the Jiangnan Orogen and western Yangtze margin of the...

The ocular surface and lacrimal gland have a frontline position in mucosal immunology. However, there have been few updates to the immune cell atlas of these tissues in recent years. To map the immune cells in murine ocular surface tissues and lacrimal gland. Central and peripheral corneas, conjunctiva, and lacrimal gland were dissociated into single cell suspensions, followed by flow cytometry. Discrepancy of immune cells between the central and peripheral corneas was compared. In the...