253 Works

Numerical investigation of asymmetric weld fusion geometry in laser welding of aluminium alloy with beam oscillation

Xi Chen, Nan Jiang, Meng Jiang, Yang Du, Shengchong Ma, Yuan Chen, Caiwang Tan, Zhenglong Lei, Sicong Zhao & Yanbin Chen
In this work, the asymmetric weld fusion geometry in oscillating laser beam welding (OLBW) of aluminium alloy was reported and investigated using a numerical approach. A multi-physics heat transfer and fluid flow model of OLBW was developed and validated with the corresponding experimental results. The weld fusion geometry, temperature fields, and fluid flow behaviours for four commonly used oscillation modes, line, circle, eight, and infinity, were calculated to analyse the origin of the asymmetric weld...

Data from: Changes in the foliar fungal community between oak leaf flushes along a latitudinal gradient in Europe

Álvaro Gaytán, Ahmed Abdelfattah, Maria Faticov, Xoaquin Moreira, Bastien Castagneyrol, Inge Van Halder, Pieter De Frenne, Camille Meeussen, Bart G. H. Timmermans, Jan P. J. G. Ten Hoopen, Pil U. Rasmussen, Nick Bos, Raimo Jaatinen, Pertti Pulkkinen, Sara Söderlund, Karl Gotthard, Katharina Pawlowski & Ayco J. M. Tack
Aim: To advance our understanding of how foliar fungal communities are structured and assembled, we assessed to what extent leaf flush and latitude can explain the within- and among-tree variation in the foliar fungal community in a foundation tree species, the pedunculate oak Quercus robur. Location: A latitudinal gradient spanning c. 20 degrees in latitude in Europe. Major taxa studied: The foliar fungal community on Quercus robur. Methods: We examined the main and interactive effects...

Additional file 6 of Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Changchuan Ye, Yuting Yang, Xi Chen, Lijie Yang, Xia Hua, Mengjie Yang, Xiangfang Zeng & Shiyan Qiao
Additional file 6: Fig S6. Standard Plot for ALA measurement. x: the absorbance at 554 nm; y: ALA concentration of sample after diluted (mg/L).

Additional file 4 of Candidate genes screening based on phenotypic observation and transcriptome analysis for double flower of Prunus mume

Huanhuan Zhu, Yan Shi, Junwei Zhang, Manzhu Bao & Jie Zhang
Additional file 4.

Additional file 1 of Phosphodiesterase 10A (PDE10A) as a novel target to suppress β-catenin and RAS signaling in epithelial ovarian cancer

Rebecca M. Borneman, Elaine Gavin, Alla Musiyenko, Wito Richter, Kevin J. Lee, David K. Crossman, Joel F. Andrews, Annelise M. Wilhite, Steven McClellan, Ileana Aragon, Antonio B. Ward, Xi Chen, Adam B. Keeton, Kristy Berry, Gary A. Piazza, Jennifer M. Scalici & Luciana Madeira da Silva
Additional file 1.

Additional file 1 of Phosphodiesterase 10A (PDE10A) as a novel target to suppress β-catenin and RAS signaling in epithelial ovarian cancer

Rebecca M. Borneman, Elaine Gavin, Alla Musiyenko, Wito Richter, Kevin J. Lee, David K. Crossman, Joel F. Andrews, Annelise M. Wilhite, Steven McClellan, Ileana Aragon, Antonio B. Ward, Xi Chen, Adam B. Keeton, Kristy Berry, Gary A. Piazza, Jennifer M. Scalici & Luciana Madeira da Silva
Additional file 1.

Palaeontological evidence for community-level decrease in mesopelagic fish size during Pleistocene climate warming in the eastern Mediterranean

Konstantina Agiadi, Frédéric Quillévéré, Rafał Nawrot, Theo Sommeville, Marta Coll, Efterpi Koskeridou, Jan Fietzke & Martin Zuschin
Mesopelagic fishes are an important element of marine food webs, a huge, still mostly untapped food resource, and great contributors to the biological carbon pump, whose future under climate change scenarios is unknown. The shrinking of commercial fishes within decades has been an alarming observation, but its causes remain contended. Here, we investigate the effect of warming climate on mesopelagic fish size in the eastern Mediterranean Sea during a glacial-interglacial-glacial transition of the Middle Pleistocene...

Multi-site assessment of reproducibility in high-content live cell imaging data

Jianjiang Hu, Xavier Serra-Picamal, Gert-Jan Bakker, Marleen Van Troys, Sabina Winograd-katz, Nil Ege, Xiaowei Gong, Yuliia Didan, Inna Grosheva, Omer Polansky, Karima Bakkali, Evelien Van Hamme, Merijn van Erp, Manon Vullings, Felix Weiss, Jarama Clucas, Anna Dowbaj, Erik Sahai, Christophe Ampe, Benjamin Geiger, Peter Friedl, Matteo Bottai & Staffan Strömblad
This dataset contains the raw images as well as the analysis pipelines and scripts used in the paper "Multi-site assessment of reproducibility in high-content live cell imaging data".
The Original data-2D.rar file contains the raw timelapse images of HT1080 cell line stably expressing H2B-EGFP and Lifeact-mCherry seeded on collagen I coated glass surface. Migration behavior of the cells was recorded in 5 min intervals for 6 h with fluorescent light microscopes equipped with environmental...

Data from: Maternal exposure to predator scents: offspring phenotypic adjustment and dispersal

Elvire Bestion, Aimeric Teyssier, Fabien Aubret, Jean Clobert & Julien Cote
Predation is a strong selective pressure generating morphological, physiological and behavioural responses in organisms. As predation risk is often higher during juvenile stages, antipredator defences expressed early in life are paramount to survival. Maternal effects are an efficient pathway to produce such defences. We investigated whether maternal exposure to predator cues during gestation affected juvenile morphology, behaviour and dispersal in common lizards (Zootoca vivipara). We exposed 21 gravid females to saurophagous snake cues for one...

Data from: Genetic signature of population fragmentation varies with mobility in seven bird species of a fragmented Kenyan cloud forest

Tom Callens, Peter Galbusera, Erik Matthysen, Eric Y Durand, Mwangi Githiru, Jeroen R Huyghe & Luc Lens
Habitat fragmentation can restrict geneflow, reduce neighbourhood effective population size, and increase genetic drift and inbreeding in small, isolated habitat remnants. The extent to which habitat fragmentation leads to population fragmentation, however, differs among landscapes and taxa. Commonly, researchers use information on the current status of a species to predict population effects of habitat fragmentation. Such methods, however, do not convey information on species-specific responses to fragmentation. Here we compare levels of past population differentiation,...

Data from: Spatially correlated extinctions select for less emigration but larger dispersal distances in the spider mite Tetranychus urticae

Emanuel A. Fronhofer, Jonas M. Stelz, Eva Lutz, Hans Joachim Poethke & Dries Bonte
Dispersal is a central process to almost all species on earth, as it connects spatially structured populations and thereby increases population persistence. Dispersal is subject to (rapid) evolution and local patch extinctions are an important selective force in this context. In contrast to the randomly distributed local extinctions considered in most theoretical studies, habitat fragmentation or other anthropogenic interventions will lead to spatially correlated extinction patterns. Under such conditions natural selection is thought to lead...

Data from: Dispersal constraints for the conservation of the grassland herb Thymus pulegioides L. in a highly fragmented agricultural landscape

An Vanden Broeck, Tobias Ceulemans, Gunter Kathagen, Maurice Hoffmann, Olivier Honnay & Joachim Mergeay
Species-rich grassland communities are one of the most important habitats for biodiversity and of high conservation priority in Europe. Restoration actions are mainly focused on the improvement of abiotic conditions, such as nutrient depletion techniques, and are generally based on the assumption that the target community will re-establish at the restored site when the target species exist in the neighborhood. Information on the contemporary seed-dispersal range is therefore crucial to develop effective conservation measures. Here,...

Data from: Epigenetic alterations and decreasing insecticide sensitivity of the Asian tiger mosquito Aedes albopictus

A. Oppold, A. Kreß, J. Vanden Bussche, J. B. Diogo, U. Kuch, J. Oehlmann, M. B. Vandegehuchte, R. Müller, M.B. Vandegehuchte & J.B. Diogo
A range of environmental factors, including chemicals, can affect epigenetic processes in organisms leading to variations in phenotype. Thus, epigenetics displays an important environmentally responsive element. The transgenerational impact of environmental stressors on DNA methylation and phenotype was the focus of this study. The influence of two known DNA methylation-changing agents, the phytoestrogen genistein and the fungicide vinclozolin, on the overall DNA methylation level in the Asian tiger mosquito Aedes albopictus was investigated. The experiment...

Data from: Low genetic diversity despite multiple introductions of the invasive plant species Impatiens glandulifera in Europe

Jenny Hagenblad, Jennifer Hülskötter, Kamal Prasad Acharya, Jörg Brunet, Olivier Chabrerie, Sara A. O. Cousins, Pervaiz A. Dar, Martin Diekmann, Pieter De Frenne, Martin Hermy, Aurélien Jamoneau, Annette Kolb, Isgard Lemke, Jan Plue, Zafar A. Reshi & Bente Jessen Graae
Background: Invasive species can be a major threat to native biodiversity and the number of invasive plant species is increasing across the globe. Population genetic studies of invasive species can provide key insights into their invasion history and ensuing evolution, but also for their control. Here we genetically characterise populations of Impatiens glandulifera, an invasive plant in Europe that can have a major impact on native plant communities. We compared populations from the species’ native...

Data from: An integrative approach to understanding the evolution and diversity of Copiapoa (Cactaceae), a threatened endemic Chilean genus from the Atacama Desert

Isabel Larridon, Helmut E. Walter, Pablo C. Guerrero, Milén Duarte, Mauricio A. Cisternas, Carol Peña Hernández, Kenneth Bauters, Pieter Asselman, Paul Goetghebeur & Marie-Stéphanie Samain
PREMISE OF THE STUDY: Species of the endemic Chilean cactus genus Copiapoa have cylindrical or (sub)globose stems that are solitary or form (large) clusters and typically yellow flowers. Many species are threatened with extinction. Despite being icons of the Atacama Desert and well loved by cactus enthusiasts, the evolution and diversity of Copiapoa has not yet been studied using a molecular approach. METHODS: Sequence data of three plastid DNA markers (rpl32-trnL, trnH-psbA, ycf1) of 39...

Data from: Genotypic diversity and differentiation among populations of two benthic freshwater diatoms as revealed by microsatellites

Pieter Vanormelingen, Katharine M. Evans, David G. Mann, Stacey Lance, Ann-Eline Debeer, Sofie D'Hondt, Tine Verstraete, Luc De Meester & Wim Vyverman
Given their large population sizes and presumed high dispersal capacity, protists are expected to exhibit homogeneous population structure over large spatial scales. On the other hand, the fragmented and short-lived nature of the lentic freshwater habitats that many protists inhabit promotes strong population differentiation. We used microsatellites in two benthic freshwater diatoms, Eunotia bilunaris ‘robust’ and Sellaphora capitata, sampled from within a pond and connected ponds, through isolated ponds from the same region to western...

Data from: The influence of balanced and imbalanced resource supply on biodiversity-functioning relationship across ecosystems

Aleksandra M. Lewandowska, Antje Biermann, Elizabeth T. Borer, Miguel A. Cebrian-Piqueras, Steven A. J. Declerck, Luc De Meester, Ellen Van Donk, Lars Gamfeldt, Daniel S. Gruner, Nicole Hagenah, W. Stanley Harpole, Kevin P. Kirkman, Christopher A. Klausmeier, Michael Kleyer, Johannes M. H. Knops, Pieter Lemmens, Eric M. Lind, Elena Litchman, Jasmin Mantilla-Contreras, Koen Martens, Sandra Meier, Vanessa Minden, Joslin L. Moore, Harry Olde Venterink, Eric W. Seabloom … & Helmut Hillebrand
Numerous studies show that increasing species richness leads to higher ecosystem productivity. This effect is often attributed to more efficient portioning of multiple resources in communities with higher numbers of competing species, indicating the role of resource supply and stoichiometry for biodiversity–ecosystem functioning relationships. Here, we merged theory on ecological stoichiometry with a framework of biodiversity–ecosystem functioning to understand how resource use transfers into primary production. We applied a structural equation model to define patterns...

Data from: Spatial dynamics and mixing of bluefin tuna in the Atlantic Ocean and Mediterranean Sea revealed using next generation sequencing

Gregory Neils Puncher, Alessia Cariani, Gregory E. Maes, Jeroen Van Houdt, Koen Herten, Rita Cannas, Naiara Rodriguez-Ezpeleta, Aitor Albaina, M. Andone Estonba, Molly Lutcavage, Alex Hanke, Jay Rooker, James S. Franks, Joseph M. Quattro, Gualtiero Basilone, Igaratza Fraile, Urtzi Laconcha, Nicolas Goñi, Ai Kimoto, A. David Macías, Francisco Alemany, Simeon Deguara, Salem W. Zgozi, Fulvio Garibaldi, Isik K. Oray … & Fausto Tinti
The Atlantic bluefin tuna is a highly migratory species emblematic of the challenges associated with shared fisheries management. In an effort to resolve the species’ stock dynamics, a genome-wide search for spatially informative single nucleotide polymorphisms (SNPs) was undertaken, by way of sequencing reduced representation libraries. An allele frequency approach to SNP discovery was used, combining the data of 555 larvae and young-of-the-year (LYOY) into pools representing major geographical areas and mapping against a newly...

Forest microclimate dynamics drive plant responses to warming

Florian Zellweger, Pieter De Frenne & David Coomes
Climate warming is causing a shift in biological communities in favor of warm-affinity species (i.e., thermophilisation). However, species responses often lag behind climate warming and local microclimates modulated by vegetation and topography are usually ignored. Here we analyze multidecadal understorey microclimate dynamics in European forests and show that thermophilisation and the climatic lag in forest plant communities are primarily controlled by microclimate. Increasing tree canopy cover reduces warming rates inside forests, but loss of canopy...

A preliminary field trial to compare control techniques for invasive Berberis aquifolium in Belgian coastal dunes

Tim Adriaens, Pieter Verschelde, Emma Cartuyvels, Bram D'hondt, Edward Vercruysse, Wouter Van Gompel, Evy Dewulf & Sam Provoost
Non-native Berberis aquifolium is notoriously invasive in Belgian coastal dunes. With its strong clonal growth through suckers, this evergreen shrub outcompetes native species and affects dune succession. To prevent further secondary spread and mitigate its impact, there was an urgent need for knowledge on the effectiveness of control measures, both at the plant and habitat level. Here, we report on a first control experiment. Individual B. aquifolium clones were subjected to one of four treatments...

Data from: Induced phenological avoidance: a neglected defense mechanism against seed predation in plants

Bram Sercu, Iris Moeneclaey, Dries Bonte & Lander Baeten
1. Flowering phenology is an important life history trait affecting plant reproductive performance and is influenced by various abiotic and biotic factors. Pre-dispersal seed predation and pollination are expected to impose counteracting selection pressure on flowering phenology, with pre-dispersal seed predation expected to favor off-peak flowering and pollination to favor synchronous flowering. 2. Here we studied the effect of pre-dispersal seed predation by the beetle Byturus ochraceus, a specialist seed herbivore, on the flowering phenology...

Because You Had a Bad Day: General and Daily Relations between Reactive Temperament, Emotion Regulation, and Depressive Symptoms in Youth

Marie-Lotte Van Beveren, Sofie Kuppens, Benjamin Hankin & Caroline Braet
Negative emotionality (NE) and positive emotionality (PE) have repeatedly shown to act as vulnerability factors for youth depression. Less research examined the mechanisms through which these reactive temperament traits may differently confer vulnerability to depression. Based on recent integrated models of depression proposing emotion regulation as a key underlying mechanism, the current study aimed to clarify the general and day-to-day relations among temperament, emotion regulation strategies, and depressive symptoms in Dutch-speaking youth (35% boys; M_age...

Saturated solute transport micro-CT dataset in Savonnières limestone

Stefanie Van Offenwert, Tom Bultreys, Marijn Boone & Veerle Cnudde

A metacommunity approach for detecting species influenced by mass effect

Thibault Leboucher, Juliette Tison-Rosebery, William R. Budnick, Aurélien Jamoneau, Wim Vyverman, Janne Soininen, Sébastien Boutry & Sophia I. Passy
1. Mass effect, allowing species to persist in unfavourable habitats, and dispersal limitation, preventing species from reaching favourable habitats, are the two major dispersal processes. While dispersal limitation can be detected by experimental or modeling techniques, mass effect is more challenging to evaluate, which hampers our ability to disentangle the influence of the environment vs. dispersal on species distribution. This is undesirable for biomonitoring programs built on known species-environment relationships. 2. We developed an approach...

Data from: A prioritised list of invasive alien species to assist the effective implementation of EU legislation

Carles Carboneras, Piero Genovesi, Montserrat Vila, Tim Blackburn, Martina Carrete, Miguel Clavero, Bram D'hondt, Jorge F. Orueta, Belinda Gallardo, Pedro Geraldes, Pablo González-Moreno, Richard D. Gregory, Wolfgang Nentwig, Jean-Yves Paquet, Petr Pysek, Wolfgang Rabitsch, Iván Ramírez, Riccardo Scalera, Jose Tella, Paul Walton, Robin Wynde & Tim M. Blackburn
1. Effective prevention and control of invasive species generally relies on a comprehensive, coherent and representative list of species that enables resources to be used optimally. European Union (EU) Regulation 1143/2014 on invasive alien species (IAS) aims to control or eradicate priority species, and to manage pathways to prevent the introduction and establishment of new IAS; it applies to species considered of Union concern and subject to formal risk assessment. So far, 49 species have...

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