351 Works

Risks and features of secondary infections in severe and critical ill COVID-19 patients

Haocheng Zhang, Yi Zhang, Jing Wu, Yang Li, Xian Zhou, Xin Li, Haili Chen, Mingquan Guo, Shu Chen, Feng Sun, Richeng Mao, Chao Qiu, Zhaoqin Zhu, Jingwen Ai & Wenhong Zhang
Objectives Severe or critical COVID-19 is associated with intensive care unit admission, increased secondary infection rate, and would lead to significant worsened prognosis. Risks and characteristics relating to secondary infections in severe COVID-19 have not been described. Methods Severe and critical COVID-19 patients from Shanghai were included. We collected lower respiratory, urine, catheters, and blood samples according to clinical necessity and culture and mNGS were performed. Clinical and laboratory data were archived. Results We found...

Toxoplasma gondii infection in white spoonbills (Platalea leucorodia) from Henan Province, China

Yurong Yang, Nan Jiang, Shilin Xin & Longxian Zhang
Toxoplasma gondii oocysts in the environment are a threat to humans and animals. This study is aimed to evaluate the prevalence of T. gondii in white spoonbills and isolate viable T. gondii from white spoonbills. In 28.6% (2/7) of white spoonbills, T. gondii antibodies were found in heart juice by the modified agglutination test (cut-off: 1:4). T. gondii DNA was detected in tissues of 42.9% (3/7) white spoonbills. One viable T. gondii strain, named TgSpoonbillCHn1,...

Defining the Syrian hamster as a highly susceptible preclinical model for SARS-CoV-2 infection

Kyle Rosenke, Kimberly Meade-White, Michael Letko, Chad Clancy, Frederick Hansen, Yanan Liu, Atsushi Okumura, Tsing-Lee Tang-Huau, Rong Li, Greg Saturday, Friederike Feldmann, Dana Scott, Zhongde Wang, Vincent Munster, Michael A. Jarvis & Heinz Feldmann
Following emergence in late 2019, SARS-CoV-2 rapidly became pandemic and is presently responsible for millions of infections and hundreds of thousands of deaths worldwide. There is currently no approved vaccine to halt the spread of SARS-CoV-2 and only very few treatment options are available to manage COVID-19 patients. For development of preclinical countermeasures, reliable and well-characterized small animal disease models will be of paramount importance. Here we show that intranasal inoculation of SARS-CoV-2 into Syrian...

Deletion of Polyamine Transport Protein PotD Exacerbates Virulence in Glaesserella (Haemophilus) parasuis in the Form of Non-biofilm-generated Bacteria in a Murine Acute Infection Model

Ke Dai, Zhen Yang, Xiaoyu Ma, Yung-Fu Chang, Sanjie Cao, Qin Zhao, Xiaobo Huang, Rui Wu, Yong Huang, Jing Xia, Qigui Yan, Xinfeng Han, Xiaoping Ma, Xintian Wen & Yiping Wen
Polyamines are small, polycationic molecules with a hydrocarbon backbone and multiple amino groups required for optimal cell growth. The potD gene, belonging to the ABC (ATP-binding cassette) transport system potABCD, encodes the bacterial substrate-binding subunit of the polyamine transport system, playing a pivotal role in bacterial metabolism and growth. The swine pathogen Glaesserella parasuis possesses an intact pot operon, and the studies presented here mainly examined the involvement of PotD in Glaesserella pathogenesis. A potD-deficient...

Circulating microRNA profile unveils mechanisms of action of acitretin for psoriasis vulgaris

Lin Chen, Jie Li, Ying Yao, Shanlong Wang, Shuangjin Zheng, Xinggang Ju & Bin Zhang
Psoriasis vulgaris is a common chronic and recurrent inflammatory skin disease. In clinical practice, acitretin is the first-line treatment drug for psoriasis vulgaris. MicroRNAs (miRNAs) play a vital role in the initiation and development of psoriasis vulgaris. However few studies focused on the mechanisms of acitretin in the treatment of psoriasis vulgaris from the perspective of miRNAs. Here, the expression profiles of circulating miRNAs in the plasma of 12 patients with psoriasis vulgaris before and...

Circulating microRNA profile unveils mechanisms of action of acitretin for psoriasis vulgaris

Lin Chen, Jie Li, Ying Yao, Shanlong Wang, Shuangjin Zheng, Xinggang Ju & Bin Zhang
Psoriasis vulgaris is a common chronic and recurrent inflammatory skin disease. In clinical practice, acitretin is the first-line treatment drug for psoriasis vulgaris. MicroRNAs (miRNAs) play a vital role in the initiation and development of psoriasis vulgaris. However few studies focused on the mechanisms of acitretin in the treatment of psoriasis vulgaris from the perspective of miRNAs. Here, the expression profiles of circulating miRNAs in the plasma of 12 patients with psoriasis vulgaris before and...

Genome-wide identification evolution and expression of vestigial-like gene family in chicken

Dan Hou, Panpan Qin, Xinran Niu, Tong Li, Bingjie Chen, Chengjie Wei, Zhenzhu Jing, Ruili Han, Hong Li, Xiaojun Liu, Yadong Tian, Donghua Li, Zhuanjian Li, Hanfang Cai & Xiangtao Kang
Vestigial-like (Vgll) genes are widespread in vertebrates and play an important role in muscle development. In this study, we used bioinformatics methods to systematically identify the chicken VGLL family in the whole genome and investigated its evolutionary history and gene structure features. Tissue expression spectra combined with real-time PCR data were used to analyze the organizational expression pattern of the genes. Based on the maximum likelihood method, a phylogenetic tree of the VGLL family was...

Prediction of intrahepatic cholestasis of pregnancy in the first 20 weeks of pregnancy

Xinyuan Zhang, Yu Chen, Stephen Salerno, Yi Li, Libin Zhou, Xiaoxi Zeng & Huafeng Li
Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease associated with a significant risk of fetal complications including pre-term delivery and fetal death. Typically, it was diagnosed in the third trimester of pregnancy. This study utilized characteristics from routine maternal examinations in the first 20 weeks’ gestation to predict ICP in pregnant women. This is a retrospective case-control study. 13,329 medical records were collected on pregnant women presenting to the West China Second University...

Long non-coding RNA RP11-283G6.5 confines breast cancer development through modulating miR-188-3p/TMED3/Wnt/β-catenin signaling

Jing Pei, Shengquan Zhang, Xiaowei Yang, Chunguang Han, Yubo Pan, Jun Li, Zhaorui Wang, Chenyu Sun & Jing Zhang
The contributions of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) to breast cancer are critical areas of investigation. In this study, we identified a novel lncRNA RP11-283G6.5 which was lowly expressed in breast cancer and whose low expression was correlated with poor overall survival and disease-free survival of breast cancer patients. Functional experiments revealed that ectopic expression of RP11-283G6.5 confined breast cancer cellular growth, migration, and invasion, and promoted cellular apoptosis. Conversely, RP11-283G6.5 silencing facilitated...

MicroRNA-146b-3p regulates the dysfunction of vascular smooth muscle cells via repressing phosphoinositide-3 kinase catalytic subunit gamma

Xijing Zhuang, Feng Gao, Lei Shi, Wei Liu, Wenjun Wang, Xuezhi He & Yang Gao
MicroRNAs are crucial regulators in the phenotype switch of vascular smooth muscle cells (VSMCs). Nonetheless, the role of miR-146b-3p in VSMCs remains unclear. In the present study, platelet-derived growth factor-BB (PDGF-BB) at different concentrations was employed to stimulate VSMCs for different times, to establish the model of VSMC dysfunction. The relative expression of miR-146b-3p was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation of VSMCs was measured by BrdU assay. Flow cytometry analysis...

Additional file 8 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 8: Table S5. Sequence of primers used in this study.

HIF-1α promotes the keloid development through the activation of TGF-β/Smad and TLR4/MyD88/NF-κB pathways

Rui Lei, Jian Li, Feng Liu, Weihan Li, Shizhen Zhang, Yang Wang, Xi Chu & Jinghong Xu
A keloid is defined as an overgrowth of the dense fibrous tissues that form around a wound. Since they destroy the vascular network, keloid tissues often exhibit anoxic conditions. Hypoxia-inducible factor-1α (HIF-1α) is a core factor that mediates hypoxia stress responses and regulates the hypoxic cellular and biological behaviors. In this study, we found that the expression level of HIF-1α in keloid tissue was significantly higher than that in the normal skin tissue. Hypoxia-induced HIF-1α...

Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis

Shangshang Hu, Jinyan Zhang, Xiaoyu Fang, Guoqing Guo, Jing Dai, Zhiyong Sheng, Dongdong Li, Jiasheng Chen, Li Zhang, Chuanmiao Liu & Yu Gao
Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of...

Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis

Shangshang Hu, Jinyan Zhang, Xiaoyu Fang, Guoqing Guo, Jing Dai, Zhiyong Sheng, Dongdong Li, Jiasheng Chen, Li Zhang, Chuanmiao Liu & Yu Gao
Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of...

Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM)

Liang Liu, Shan Xiao, Yan Wang, Zifeng Zhu, Yiyao Cao, Sen Yang, Rongkang Mai & Yong Zheng
Circular RNAs (circRNAs) are closely associated with cancer development in glioblastoma (GBM), and this study aims to explore the molecular mechanisms of a novel circular RNA circZNF652 in regulating GBM aggressiveness. The present study found that CircZNF652 and SERPINE1 were upregulated, while miR-486-5p was downregulated in GBM tissues and cell lines, and GBM patients with high expression of CircZNF652 and SERPINE1, and patients with low expression of miR-486-5p tended to have a worse prognosis. Further...

Downregulation of Smad4 expression confers chemoresistance against imatinib mesylate to chronic myeloid leukemia K562 cells

Jiangzhao Zhang, Min Zhang, Yan Liang, Min Liu & Zhiping Huang
Objective: Imatinib mesylate (IM), a tyrosine kinase inhibitor, exhibits clinically prominent effects against chronic myeloid leukemia (CML); however, a few patients have shown resistance to IM treatment, resulting in disease progression. Smad4 is a tumor inhibitor that transduces TGF-β signaling and modulates genomic stability. Previous studies have indicated that decreased Smad4 expression played a bidirectional role in chemosensitivity in many types of cancers. Therefore, this study aims to evaluate the association between IM sensitivity and...

Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae in China

Xuemei Yang, Qiaoling Sun, Jiaping Li, Yu Jiang, Yi Li, Jianping Lin, Kaichao Chen, Edward Wai-Chi Chan, Rong Zhang & Sheng Chen
The epidemiological features of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and its potential threat to human health are currently unknown. In this study, a total of 784 blaKPC-2-bearing CRKP strains collected from three hospitals located at different geographical locales in China during 2014–2017 were subjected to molecular typing, screening of virulence plasmid, string test and WGS (367/784 strains). The proportion of CRKP among all clinical K. pneumoniae strains increased sharply in China during...

Molecular epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae in China

Xuemei Yang, Qiaoling Sun, Jiaping Li, Yu Jiang, Yi Li, Jianping Lin, Kaichao Chen, Edward Wai-Chi Chan, Rong Zhang & Sheng Chen
The epidemiological features of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and its potential threat to human health are currently unknown. In this study, a total of 784 blaKPC-2-bearing CRKP strains collected from three hospitals located at different geographical locales in China during 2014–2017 were subjected to molecular typing, screening of virulence plasmid, string test and WGS (367/784 strains). The proportion of CRKP among all clinical K. pneumoniae strains increased sharply in China during...

Subspace Estimation with Automatic Dimension and Variable Selection in Sufficient Dimension Reduction

Jing Zeng, Qing Mai & Xin Zhang
Sufficient dimension reduction (SDR) methods target finding lower-dimensional representations of a multivariate predictor to preserve all the information about the conditional distribution of the response given the predictor. The reduction is commonly achieved by projecting the predictor onto a low-dimensional subspace. The smallest such subspace is known as the Central Subspace (CS) and is the key parameter of interest for most SDR methods. In this article, we propose a unified and flexible framework for estimating...

Composition, Age, and Origin of Ordovician-Devonian Tanjianshan granitoids in the North Qaidam Orogenic Belt of northern Tibet: Implications for Tectonic Evolution

Chenghan Xu, Fengyue Sun, Xingzhu Fan, Lu Yu, Depeng Yang, Shahzad Bakht & Dongqian Wu
The North Qaidam Orogenic Belt (NQOB) is characterized by widespread Palaeozoic magmatism and high to ultrahigh-pressure metamorphism that provides important information regarding the continental crust growth and tectonic evolution in the North Qaidam. Our work focuses on the geology, geochronology, whole-rock geochemistry, and Sr–Nd isotopy of granitoids in the Tanjianshan area in the northern NQOB. The results indicate that the 486–469 Ma adakitic and TTG-like granitoids originated from the partial melting of Middle–Late Mesoproterozoic lower...

Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation

Anli Yang, Huadi Chen, Jianwei Lin, Ming Han, Xiaopeng Yuan, Tao Zhang, Qingwei Nian, Mengran Peng, Dian Li, Chenglin Wu & Xiaoshun He
The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with...

Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation

Anli Yang, Huadi Chen, Jianwei Lin, Ming Han, Xiaopeng Yuan, Tao Zhang, Qingwei Nian, Mengran Peng, Dian Li, Chenglin Wu & Xiaoshun He
The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with...

Gapless genome assembly of East Asian finless porpoise, Neophocaena asiaeorientalis sunameri

Denghua Yin, Chunhai Chen, Danqing Lin, Jialun Zhang, Congping Ying, Yan Liu, Wang Liu, Zhichen Cao, Chenxi Zhao, Chenhe Wang, Liping Liang, Pao Xu, Jianbo Jian & Kai Liu
There are the genome sequence, gene set, coding sequence and protein sequence of Neophocaena asiaeorientalis sunameri.

Platelet–Lymphocyte Ratio, Neutrophil–Lymphocyte Ratio and Their Dynamic Changes with Type 2 Diabetes Mellitus: A Cohort Study in China

Canjia Zhang, Huan Chen, Shangxin Cui, Yeli Lin, Yongqiang Liang, Ping Zhao, Changyi Wang, Shan Xu, Xiaolin Peng, Hongen Chen, Li Wang, Dan Zhao, Ming Zhang, Dongsheng Hu, Yanmei Lou & Fulan Hu
This study aimed at investigating the relationships between Platelet-Lymphocyte ratio (PLR) and Neutrophil–Lymphocyte ratio (NLR) and their dynamic changes (∆PLR, ∆NLR) with type 2 diabetes mellitus (T2DM) in a Chinese cohort study. This study recruited 41,439 individuals who were diagnosed without T2DM at first health examination and completed at least one follow-up. The relationships between NLR, PLR, ∆PLR, ∆NLR and T2DM risk were analyzed using the Cox regression model with corresponding Hazard Ratios (HRs) and...

Additional file 2 of Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Xuewen Zhu, Yaolei Mi, Xiangxiao Meng, Yiming Zhang, Weiqiang Chen, Xue Cao, Huihua Wan, Wei Yang, Jun Li, Sifan Wang, Zhichao Xu, Atia Tul Wahab, Shilin Chen & Wei Sun
Additional file 2: Table S2. Quantitative primers of the selected enzyme-encoding genes of flavonoid metabolic pathway in C. sativa.

Registration Year

  • 2023
    22
  • 2022
    247
  • 2021
    61
  • 2020
    12
  • 2019
    7
  • 2018
    2

Resource Types

  • Dataset
    351

Affiliations

  • Southwest Medical University
    351
  • Shanghai Jiao Tong University
    247
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    246
  • Zhejiang University
    244
  • Sichuan University
    243
  • Fudan University
    217
  • Huazhong University of Science and Technology
    205
  • Sun Yat-sen University
    192
  • Peking University
    180
  • Chinese Academy of Sciences
    173