2 Works

β-cell-specific deletion of Zfp148 improves nutrient-stimulated β-cell Ca2+ responses

Christopher Emfinger, Alan Attie, Matthew Merrins, Eleonora De Klerk, Matthias Hebrok, Richard Kibbey, Rebecca Cardone, Kathryn Schueler, Mary Rabaglia, Shane Simonett, Donnie Stapleton, Kelly Mitok, Sophie Lewandowski, Ziyue Wang, Christina Kendziorski, Mark Keller, Hannah Foster, Stephen Gygi, Xinyue Liu, Joao Paulo, Qinq Yu & José Perales
Insulin secretion from pancreatic β-cells is essential for glucose homeostasis. An insufficient response to the demand for insulin results in diabetes. We previously showed that β-cell-specific deletion of Zfp148 (β-Zfp148KO) improves glucose tolerance and insulin secretion in mice. Here, we performed Ca2+ imaging of islets from β‑Zfp148KO and control mice on both a chow and a Western-style diet. β-Zfp148KO islets demonstrate improved sensitivity and sustained Ca2+ oscillations in response to elevated glucose. β-Zfp148KO islets also...

Supplemental material for: The estrogen receptor α cistrome in human endometrium and epithelial organoids

Sylvia Hewitt, San-Pin Wu, Tianyuang Wang, Madhumita Ray, Marja Brolinson, Steven Young, Thomas Spencer, Alan DeCherney & Francsco DeMayo
Endometrial health is impacted by molecular processes that underlie estrogen responses. We assessed estrogen regulation of endometrial function by integrating the estrogen receptor alpha (ESR1) cistromes and transcriptomes of endometrial biopsies taken from the proliferative and mid-secretory phases of the menstrual cycle together with hormonally stimulated endometrial epithelial organoids. The cycle stage specific ESR1 binding sites were determined by ChIPseq and then integrated with changes in gene expression from RNAseq data to infer candidate ESR1...

Registration Year

  • 2022
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  • Dataset
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Affiliations

  • National Institute of Environmental Health Sciences
    2
  • University of North Carolina
    1
  • University of Wisconsin-Madison
    1
  • University of Wisconsin–Madison
    1
  • University of Barcelona
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  • University of Missouri
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  • Yale University
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  • Harvard University
    1
  • University of California, San Francisco
    1
  • National Institute of Child Health and Human Development
    1