23 Works

Random forests for predicting species identity of forensically important blow flies (Diptera: Calliphoridae) and flesh flies (Sarcophagidae) using geometric morphometric data: proof of concept

Tsung Fei Khang, Nur Ayuni Dayana Mohd Puaad, Ser Huy Teh & Zulqarnain Mohamed
Wing shape variation has been shown to be useful for delineating forensically important fly species in two Diptera families: Calliphoridae and Sarcophagidae. Compared to DNA-based identification, the cost of geometric morphometric data acquisition and analysis is relatively much lower because the tools required are basic, and stable softwares are available. However, to date, an explicit demonstration of using wing geometric morphometric data for species identity prediction in these two families remains lacking. Here, geometric morphometric...

Data from: Phylogeography and demographic history of two widespread Indo-Pacific mudskippers (Gobiidae: Periophthalmus)

Gianluca Polgar, Lorenzo Zane, Massimiliano Babbucci, Federica Barbisan, Tomaso Patarnello, Lukas Rüber & Chiara Papetti
This study provides a first description of the phylogeographic patterns and evolutionary history of two species of the mudskipper genus Periophthalmus. These amphibious gobies are distributed throughout the whole Indo-Pacific region and Atlantic coast of Africa, in peritidal habitats of soft-bottom coastal ecosystems. Three sequence datasets of two widely distributed species, Periophthalmus argentilineatus and P. kalolo, were obtained by amplifying and sequencing two mtDNA markers (D-loop and 16S rDNA) and the nDNA rag1 region. The...

Data from: Comparative phylogeography reveals a shared impact of Pleistocene environmental change in shaping genetic diversity within nine Anopheles mosquito species across the Indo-Burma biodiversity hotspot

Katy Morgan, Samantha M. O’Loughlin, Bin Chen, Yvonne-Marie Linton, Damrongpan Thongwat, Pradya Somboon, Mun Yik Fong, Roger Butlin, Robert Verity, Anil Prakash, Thaung Hlaing, Simone Nambanya, Duong Socheat, Trung Ho Dinh & Catherine Walton
Southeast Asia is one of the world’s richest regions in terms of biodiversity. An understanding of the distribution of diversity and the factors shaping it is lacking, yet essential for identifying conservation priorities for the region’s highly threatened biodiversity. Here we take a large scale comparative approach, combining data from nine forest associated Anopheles mosquito species and using statistical phylogeographic methods to disentangle the effects of environmental history, species specific ecology, and random coalescent effects....

Data from: DNA barcoding reveals the coral “laboratory-rat”, Stylophora pistillata encompasses multiple identities

Shashank Keshavmurthy, Sung-Yin Yang, Ada Alamaru, Yao-Yang Chuang, Michel Pichon, David Obura, Silvia Fontana, Stephane De Palmas, Fabrizio Stefani, Francesca Benzoni, Angus MacDonald, Annika M. E. Noreen, Chienshun Chen, Carden C. Wallace, Ruby M. Pillay, Vianney Denis, Affendi Yang Amri, James D. Reimer, Takuma Mezaki, Charles Sheppard, Yossi Loya, Avidor Abelson, Mohammed S. Mohammed, Andrew C. Baker, Pargol G. Mostafavi … & Chaolun A. Chen
Stylophora pistillata is a widely used coral “lab-rat” species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacific-Western Australia, Chagos-Madagascar-South Africa, Gulf of Aden-Zanzibar- Madagascar, and Red Sea-Persian/Arabian Gulf-Kenya. On the basis of the fossil record of Stylophora, these four...

Data from: Extant primitively segmented spiders have recently diversified from an ancient lineage

Xin Xu, Fengxiang Liu, Ren-Chung Cheng, Jian Chen, Xiang Xu, Zhisheng Zhang, Hirotsugu Ono, Dinh Sac Pham, Y. Norma-Rashid, Miquel A. Arnedo, Matjaž Kuntner, Daiqin Li & R.-C. Cheng
Living fossils are lineages that have retained plesiomorphic traits through long time periods. It is expected that such lineages have both originated and diversified long ago. Such expectations have recently been challenged in some textbook examples of living fossils, notably in extant cycads and coelacanths. Using a phylogenetic approach, we tested the patterns of the origin and diversification of liphistiid spiders, a clade of spiders considered to be living fossils due to their retention of...

Data from: Aggressive spiders make the wrong decision in a difficult task

Chia-Chen Chang, Zhi Yun Lim, Danielle A. Klomp, Yusoff Norma-Rashid & Daiqin Li
Accurate and timely decisions are critical for foraging, predator avoidance, and reproductive success. However, there is often a trade-off between speed and accuracy in decision-making, where individuals that make decisions more quickly make more mistakes. An individual’s personality may influence its decision-making style (i.e. whether it errs more in the speed or accuracy of a decision) and this relationship may change depending on contexts. Despite growing research on invertebrate personality, how personality correlates with decision-making...

Data from: Resource availability and roosting ecology shape reproductive phenology of rain forest insectivorous bats

Elias Nurul-Ain, Rosli Hashim, Tigga Kingston & Hashim Rosli
Bats in temperate and subtropical regions typically synchronize birth of a single young with peaks in resource availability driven by local climate patterns. In tropical rain forest, insects are available throughout the year, potentially allowing departures from seasonal monoestry. However, reproductive energy budgets may be constrained by the cost of commuting to foraging grounds from distant roosts. To test these hypotheses, we simultaneously tracked female reproductive activity of 11 insectivorous bat species, insect biomass, and...

Dispersive liquid-liquid microextraction with extractant removal by magnetic nanoparticles for chloramphenicol preconcentration in water samples

Noorfatimah Yahaya, , Nur Afiqah Aling, Mazidatulakmam Miskam, Mardiana Saaid, Nur Nadhirah Mohamad Zain, Sazlinda Kamaruzaman, Muggundha Raoov, Nor Suhaila Mohamad Hanapi & Wan Nazihah Wan Ibrahim
This work describes the development of a new methodology based on dispersive liquid-liquid microextraction with extractant removal by magnetic nanoparticles (DLLME-MNPs) for preconcentration and extraction of chloramphenicol (CAP) antibiotic residues in water. The approach is based on the use of decanoic acid as the extraction solvent followed by the application of MNPs to magnetically retrieve the extraction solvent containing the extracted CAP. The coated MNPs were then desorbed with methanol, and the clean extract was...

Data from: Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah)

Choo Hock Tan, Kae Yi Tan, Shin Yee Fung & Nget Hong Tan
Background: The king cobra (Ophiophagus hannah) is widely distributed throughout many parts of Asia. This study aims to investigate the complexity of Malaysian Ophiophagus hannah (MOh) venom for a better understanding of king cobra venom variation and its envenoming pathophysiology. The venom gland transcriptome was investigated using the Illumina HiSeq™ platform, while the venom proteome was profiled by 1D-SDS-PAGE-nano-ESI-LCMS/MS. Results: Transcriptomic results reveal high redundancy of toxin transcripts (3357.36 FPKM/transcript) despite small cluster numbers, implying...

Data from: Brain size evolution in pipefishes and seahorses: the role of feeding ecology, life history and sexual selection

Masahito Tsuboi, Adam Chee Ooi Lim, Boon Leong Ooi, Mei Yee Yip, Ving Ching Chong, Ingrid Ahnesjö & Niclas Kolm
Brain size varies greatly at all taxonomic levels. Feeding ecology, life history and sexual selection have been proposed as key components in generating contemporary diversity in brain size across vertebrates. Analyses of brain size evolution have, however, been limited to lineages where males predominantly compete for mating and females choose mates. Here, we present the first original data set of brain sizes in pipefishes and seahorses (Syngnathidae) a group in which intense female mating competition...

Data from: Monogenean anchor morphometry: systematic value, phylogenetic signal, and evolution

Tsung Fei Khang, Oi Yoon Michelle Soo, Wooi Boon Tan & Lee Hong Susan Lim
Background. Anchors are one of the important attachment appendages for monogenean parasites. Common descent and evolutionary processes have left their mark on anchor morphometry, in the form of patterns of shape and size variation useful for systematic and evolutionary studies. When combined with morphological and molecular data, analysis of anchor morphometry can potentially answer a wide range of biological questions. Materials and Methods. We used data from anchor morphometry, body size and morphology of 13...

Data from: Conflict of interest and signal interference lead to the breakdown of honest signalling

Roman Popat, Eric Pollitt, Freya J. G. Harrison, Hardeep Naghra, Kar-Wai Hong, Kok-Gan Chan, Ashleigh S. Griffin, Paul Williams, Sam P. Brown, Stuart A. West, Stephen P. Diggle, Eric J. G. Pollitt & Freya Harrison
Animals use signals to coordinate a wide range of behaviours, from feeding offspring to predator avoidance. This poses an evolutionary problem, because individuals could potentially signal dishonestly to coerce others into behaving in ways that benefit the signaller. Theory suggests that honest signalling is favoured when individuals share a common interest and signals carry reliable information. Here, we exploit the opportunities offered by bacterial signalling, to test these predictions with an experimental evolution approach. We...

Data from: New sporopollenin-based β-cyclodextrin functionalized magnetic hybrid adsorbent for magnetic solid-phase extraction of nonsteroidal anti-inflammatory drugs from water samples.

Syed Fariq Fathullah Syed Yaacob, Muhammad Afzal Kamboh, Wan Aini Wan Ibrahim & Sharifah Mohamad
A magnetic solid-phase extraction (MSPE) procedure on the newly synthesized magnetic β-cyclodextrin functionalized with toluene diisocyanate (TDI) as a linker and was further modified with bio-polymeric spores of sporopollenin (MSp-TDI-βCD) was developed for the extraction of nonsteroidal anti-inflammatory drugs (NSAIDs) namely indoprofen (INP), ketoprofen (KTP), ibuprofen (IBP) and fenoprofen (FNP) from water samples prior to their HPLC-DAD determination. The newly synthesised MSp-TDI-βCD was comprehensibly characterized using FT-IR, XRD, SEM-EDX, BET and VSM analyses. The separation...

Data from: Population genomics reveals additive and replacing horizontal gene transfers in the emerging pathogen Dickeya solani

Slimane Khayi, Pauline Blin, Jacques Pédron, Teik-Min Chong, Kok-Gan Chan, Mohieddine Moumni, Valérie Hélias, Frédérique Van Gijsegem & Denis Faure
Background: Dickeya solani is an emerging pathogen that causes soft rot and blackleg diseases in several crops including Solanum tuberosum, but little is known about its genomic diversity and evolution. Results: We combined Illumina and PacBio technologies to complete the genome sequence of D. solani strain 3337 that was used as a reference to compare with 19 other genomes (including that of the type strain IPO2222 T ) which were generated by Illumina technology. This...

Data from: Factors affecting Japanese retirees’ healthcare service utilisation in Malaysia – a qualitative study

Ayako Kohno, Nik Daliana Nik Farid, Ghazali Musa, Norlaili Abdul Aziz, Takeo Nakayama & Maznah Dahlui
Objective: Living overseas in another culture, the retirees need to adapt to new environment but often this causes difficulties, particularly among the elderly who are having health problems. This study aims to examine factors affecting healthcare service utilisation among Japanese retirees in Malaysia. Design: We conducted focus group discussions with Japanese retirees and in-depth interviews with government officials, travel agents, hospital managers and Japanese interpreters in the selected private hospitals. Guided by the Andersen Healthcare...

Data from: Ecomorphological adaptation in three mudskippers (Teleostei: Gobioidei: Gobiidae) from the Persian Gulf and the Gulf of Oman

Gianluca Polgar, Mehdi Ghanbarifardi, Salvatore Milli, Ainhoa Agorreta, Mansour Aliabadian, Hamid Reza Esmaeili & Tsung Fei Khang
We hypothesise that the body shapes of three mudskipper species (Boleophthalmus dussumieri, Periophthalmus waltoni, and Scartelaos tenuis) are ecomorphological adaptations to different epi- and infaunal habitats. We investigated: (i) the association between burrow density and selected ecological variables; (ii) the phylogenetic relationships among these species, based on two mtDNA and one nDNA markers; (iii) their geometric morphometrics and ancestral shape reconstructions, based on two-dimensional landmark configurations; and (iv) their body surface-to-volume ratios (SAV), based on...

Data from: The prevalence of c.2268dup and detection of two novel alterations, c.670_672del and c.1186C>T, in the TPO gene in a cohort of Malaysian-Chinese with thyroid dyshormonogenesis

Ching Chin Lee, Fatimah Harun, Muhammad Yazid Jalaludin, Choon Han Heh, Rozana Othman & Sarni Mat Junit
Objectives: The c.2268dup mutation in the thyroid peroxidase (TPO) gene is the most common TPO alteration reported in Taiwanese patients with thyroid dyshormonogenesis. The ancestors of these patients are believed to originate from the southern province of China. Our previous study showed that this mutation leads to reduced abundance of the TPO protein and loss of TPO enzyme activity in a Malaysian-Chinese family with goitrous hypothyroidism. The aim of our study was to provide further...

Data from: Association between night-shift work, sleep quality, and health-related quality of life : a cross-sectional study among manufacturing workers in a middle-income setting

Yin Cheng Lim, C W HOE Victor, Bhoo Pathy Nirmala & AZLAN DARUS
Objectives: Night-shift work may adversely affect health. This study aimed to determine the impact of night-shift work on health-related quality of life (HRQoL), and assess whether sleep quality was a mediating factor. Design: Cross-sectional study. Setting: 11 manufacturing factories in Malaysia. Participants: 177 night-shift workers aged 40 to 65 years old were compared with 317 non-night-shift work. Primary and secondary outcomes: Participants completed a self-administered questionnaire on socio-demography and lifestyle factors, short Form-12v2 Health Survey...

Phylogenomic analysis of ultraconserved elements resolves the evolutionary and biogeographic history of Segmented Trapdoor Spiders

Xin Xu, Yong-Chao Su, Simon Y. W. Ho, Matjaž Kuntner, Hirotsugu Ono, Fengxiang Liu, Chia-Chen Chang, Natapot Warrit, Varat Sivayyapram, Khin Pyae Pyae Aung, Dinh Sac Pham, Y. Norma-Rashid & Daiqin Li
The segmented trapdoor spiders (Liphistiidae) are the sole surviving family of the suborder Mesothelae, which forms the sister lineage to all other living spiders. Liphistiids have retained a number of plesiomorphic traits and their present-day distribution is limited to East and Southeast Asia. Studying this group has the potential to shed light on the deep evolutionary history of spiders, but the phylogeny and divergence times of the family have not been resolved with confidence. We...

A linkage-based genome assembly for the mosquito Aedes albopictus and identification of chromosomal regions affecting diapause

John Boyle, Peter Armbruster, Pasi Rastas, Xin Huang, Austin Garner, Indra Vythilingam & Peter Armbruster
The Asian tiger mosquito, Aedes albopictus, is an invasive vector mosquito of substantial public health concern. The large genome size (~1.19-1.28 Gb by cytofluorometric estimates), comprised of ~68% repetitive DNA sequences, has made it difficult to produce a high-quality genome assembly for this species. We constructed a high-density linkage map for Ae. albopictus based on 111,328 informative SNPs obtained by RNAseq. We then performed a linkage-map anchored reassembly of AalbF2, the genome assembly produced by...

Rheological and thermal degradation properties of hyperbranched polyisoprene prepared by anionic polymerisation

Shehu Habibu, Norazilawati Muhamad Sarih, Nor Asrina Sairi & Muzafar Zulkifli
Hyperbranched polyisoprene was prepared by anionic copolymerisation under high vacuum condition. Size exclusion chromatography (SEC) was used to characterise the molecular weight and branching nature of these polymers. The characterisation by differential scanning calorimetry (DSC) and melt rheology indicated lower Tg and complex viscosity in the branched polymers as compared to the linear polymer. Degradation kinetics of these polymers was explored using thermogravimetric analysis (TGA) via non-isothermal techniques. The polymers were heated under nitrogen from...

Elasticity and damping ratio measurement of droplets on super-hydrophobic surfaces

Yukai Sun, Yelong Zheng, Le Song, Peiyuan Sun, Meirong Zhao, Yixiong Zhou & Clarence Augustine TH Tee
The elasticity of the droplet refers to the ability of the droplet in restoring to its original shape and ensuring its strong robustness. The measurement of the droplets' elasticity is vitally important in understanding the characteristics and mechanism of the droplet jumping, spreading, and coalescence. In this paper, we measured the elasticity and damping ratio of droplets with different viscosity. A plate coated with a hydrophobic layer pressed on a droplet, and the elastic force...

Data from: Comparative phylogeography reveals a shared impact of Pleistocene environmental change in shaping genetic diversity within nine Anopheles mosquito species across the Indo-Burma biodiversity hotspot

Katy Morgan, Samantha M. O’Loughlin, Bin Chen, Yvonne-Marie Linton, Damrongpan Thongwat, Pradya Somboon, Mun Yik Fong, Roger Butlin, Robert Verity, Anil Prakash, Thaung Hlaing, Simone Nambanya, Duong Socheat, Trung Ho Dinh & Catherine Walton
Southeast Asia is one of the world’s richest regions in terms of biodiversity. An understanding of the distribution of diversity and the factors shaping it is lacking, yet essential for identifying conservation priorities for the region’s highly threatened biodiversity. Here we take a large scale comparative approach, combining data from nine forest associated Anopheles mosquito species and using statistical phylogeographic methods to disentangle the effects of environmental history, species specific ecology, and random coalescent effects....

Registration Year

  • 2022
    1
  • 2021
    2
  • 2020
    3
  • 2019
    1
  • 2018
    2
  • 2017
    1
  • 2016
    4
  • 2015
    4
  • 2014
    2
  • 2013
    1

Resource Types

  • Dataset
    23

Affiliations

  • University of Malaya
    23
  • National University of Singapore
    3
  • Chongqing Normal University
    2
  • Regional Medical Research Centre
    2
  • University of Manchester
    2
  • Universiti Brunei Darussalam
    2
  • Vietnam Academy of Science and Technology
    2
  • Natural History Museum
    2
  • Hubei University
    2
  • Cambodia National Malaria Center
    2