Objective: To determine predictors of alcohol responsiveness in a large cohort of dystonia patients. Methods: 2159 participants with dystonia were prospectively enrolled in the cross-sectional Dystonia Coalition multicenter study. Patients with secondary, combined or confirmed genetic dystonia (total n=164) or unknown alcohol responsiveness (n= 737) were excluded. Patients answered a standardized questionnaire and were clinically examined using a standardized video protocol and the Burke-Fahn-Marsden Dystonia Rating Scale. Alcohol responsiveness was determined by patients’ self-report. Results:...
Heterochromatin-dependent transcription of satellite DNAs in the Drosophila melanogaster female germlineXiaolu Wei, Danna G. Eickbush, Iain Speece & Amanda M. Larracuente
Large blocks of tandemly repeated DNAs—satellite DNAs (satDNAs)—play important roles in heterochromatin formation and chromosome segregation. We know little about how satDNAs are regulated, however their misregulation is associated with genomic instability and human diseases. We use the Drosophila melanogaster germline as a model to study the regulation of satDNA transcription and chromatin. Here we show that complex satDNAs (>100-bp repeat units) are transcribed into long noncoding RNAs and processed into piRNAs (PIWI interacting RNAs)....
Data from: Non-invasive monitoring of temporal and spatial blood flow during bone graft healing using diffuse correlation spectroscopySongfeng Han, Michael D. Hoffman, Ashley R. Proctor, Emmanuel A. Mannoh, Nathaniel E. Barber, Hyun Jin Kim, Ki Won Jung, Danielle S. W. Benoit, Regine Choe & Joseph B. Vella
Vascular infiltration and associated alterations in microvascular blood flow are critical for complete bone graft healing. Therefore, real-time, longitudinal measurement of blood flow has the potential to successfully predict graft healing outcomes. Herein, we non-invasively measure longitudinal blood flow changes in bone autografts and allografts using diffuse correlation spectroscopy in a murine femoral segmental defect model. Blood flow was measured at several positions proximal and distal to the graft site before implantation and every week...
Data from: First-in-human randomized controlled trial of an oral, replicating Adenovirus 26 vector vaccine for HIV-1Kathryn E. Stephenson, Michael C. Keefer, Catherine A. Bunce, Doreen Frances, Peter Abbink, Lori F. Maxfield, George H. Neubauer, Joseph Nkolola, Lauren Peter, Christopher Lane, Harriet Park, Carl Verlinde, Angela Lombardo, Christopher Yallop, Menzo Havenga, Patricia Fast, John Treanor & Dan H. Barouch
Background: Live, attenuated viral vectors that express HIV-1 antigens are being investigated as an approach to generating durable immune responses against HIV-1 in humans. We recently developed a replication-competent, highly attenuated Ad26 vector that expresses mosaic HIV-1 Env (rcAd26.MOS1.HIV-Env, “rcAd26”). Here we present the results of a first-in-human, placebo-controlled clinical trial to test the safety, immunogenicity and mucosal shedding of rcAd26 given orally. Methods: Healthy adults were randomly assigned to receive a single oral dose...
Optimizing parameters for using the parallel auditory brainstem response (pABR) to quickly estimate hearing thresholdsMelissa Polonenko & Ross Maddox
Objectives: Timely assessments are critical to providing early intervention and better hearing and spoken language outcomes for children with hearing loss. To facilitate faster diagnostic hearing assessments in infants, the authors developed the parallel auditory brainstem response (pABR), which presents randomly timed trains of tone pips at five frequencies to each ear simultaneously. The pABR yields high-quality waveforms that are similar to the standard, single-frequency serial ABR but in a fraction of the recording time....
Data from: Human retinal pigment epithelium: in vivo cell morphometry, multispectral autofluorescence, and relationship to cone mosaicCharles E. Granger, Qiang Yang, Hongxin Song, Kenichi Saito, Koji Nozato, Lisa R. Latchney, Bianca T. Leonard, Mina M. Chung, David R. Williams & Ethan A. Rossi
Purpose: To characterize in vivo morphometry and multispectral autofluorescence of the retinal pigment epithelial (RPE) cell mosaic and its relationship to cone cell topography across the macula. Methods: RPE cell morphometrics were computed in regularly spaced regions of interest (ROIs) from contiguous short-wavelength autofluorescence (SWAF) and photoreceptor reflectance images collected across the macula in one eye of 10 normal participants (23–65 years) by using adaptive optics scanning light ophthalmoscopy (AOSLO). Infrared autofluorescence (IRAF) images of...
Dynamic evolution of euchromatic satellites on the X chromosome in Drosophila melanogaster and the simulans cladeJ.S. Sproul, D.E. Khost, D.G. Eickbush, S. Negm, X. Wei, I. Wong & A.M. Larracuente
ABSTRACTSatellite DNAs (satDNAs) are among the most dynamically evolving components of eukaryotic genomes and play important roles in genome regulation, genome evolution, and speciation. Despite their abundance and functional impact, we know little about the evolutionary dynamics and molecular mechanisms that shape satDNA distributions in genomes. Here we use high-quality genome assemblies to study evolutionary dynamics of two complex satDNAs, Rsp-like and 1.688 gm/cm3, in Drosophila melanogaster and its three nearest relatives in the simulans...
Data from: Telemedicine in neurology: telemedicine work group of the American Academy of Neurology updateJaime M. Hatcher-Martin, Jamie Lynn Adams, Eric R Anderson, Riley Bove, Tamika M. Burrus, Mahan Chehrenama, Mary Dolan O'Brien, Dawn S. Eliashiv, Deniz Erten-Lyons, Barbara S. Giesser, Lauren Moo, Pushpa Narayanaswami, Marvin A. Rossi, Madhu Soni, Nauman Tariq, Jack W. Tsao, Bert Vargas, Scott Vota, Scott Wessels, Hannah Planalp & Raghav Govindarajan
ABSTRACT Purpose: While there is strong evidence supporting the importance of telemedicine in stroke, its role in other areas of neurology is not as clear. The goal of this review is to provide an overview of evidence-based data regarding the role of teleneurology in the care of patients with neurologic disorders other than stroke. Recent findings: Studies across multiple specialties report noninferiority of evaluations by telemedicine compared with traditional, in-person evaluations in terms of patient...
Data from: Environmental dependency of amphibian-ranavirus genotypic interactions: evolutionary perspectives on infectious diseasesPierre Echaubard, Joel Leduc, Bruce Pauli, V. Gregory Chinchar, Jacques Robert & David Lesbarrères
The context-dependent investigations of host–pathogen genotypic interactions, where environmental factors are explicitly incorporated, allow the assessment of both coevolutionary history and contemporary ecological influences. Such a functional explanatory framework is particularly valuable for describing mortality trends and identifying drivers of disease risk more accurately. Using two common North American frog species (Lithobates pipiens and Lithobates sylvaticus) and three strains of frog virus 3 (FV3) at different temperatures, we conducted a laboratory experiment to investigate the...
Data from: Acute cytotoxicity and increased vascular endothelial growth factor after in vitro nitrogen mustard vapor exposureMatthew McGraw, So‐Young Kim, Carl W. White & Livia A. Veress
RATIONALE: Nitrogen mustard (NM) is a highly toxic alkylating agent. It is classified as chemical threat due to its potential use as a warfare agent. When inhaled, mustard exposure can cause both acute and chronic lung injury. The aims of this study are to develop an in vitro culture system for modeling mustard-induced airway injury and to identify growth factors contributing to airway pathology. METHODS: Primary human bronchial epithelial cells co-cultured with primary human pulmonary...
Objective Hospitals participating in the Medicare Shared Savings Program (MSSP) share with the Centers for Medicare and Medicaid Services (CMS) the savings generated by reducing readmissions. Our aim was to determine whether MSSP is associated with changes in readmissions and mortality for Medicare patients hospitalized with ischemic stroke, and whether MSSP has a different impact on safety-net hospitals (SNHs) compared to non-SNHs. Methods This study was based on the CMS Hospital Compare data for risk-standardized...
University of Rochester Medical Center11
University of Rochester5
Rush University Medical Center2
Beth Israel Deaconess Medical Center2
University of Tennessee Health Science Center1
Massachusetts General Hospital1
Oregon Health & Science University1
American Academy of Neurology1
Assistance Publique -Hopitaux De Paris1
University of Maryland Medical Center1