382 Works

Circular RNA coiled-coil domain containing 66 regulates malignant development of papillary thyroid carcinoma by upregulating La ribonucleoprotein 1 via the sponge effect on miR-129-5p

Peipei Li, Junhui Chen, Jun Zou, Wei Zhu, Yan Zang & Hongwu Li
Circular RNAs (circRNAs) play vital roles in the development and progression of various diseases. CircRNA coiled-coil domain containing 66 (circ-CCDC66) has been reported to be involved in several cancers, but its biological function and underlying mechanism in papillary thyroid carcinoma (PTC) remain unclear. We detected the relative expression level of circ-CCDC66 in PTC specimens and cell lines using real-time reverse transcription PCR. In addition, EdU assay, transwell assay, and xenograft analysis were performed to measure...

Establishment of a subcutaneous adipogenesis model and distinct roles of LKB1 regulation on adipocyte lipid accumulation in high-altitude Bos grunniens

Yongqi Yue, Yonglin Hua, Jing Zhang, Yu Guo, Dan Zhao, Wentao Huo, Yan Xiong, Fenfen Chen, Yaqiu Lin, Xianrong Xiong & Jian Li
The domestic yak was regarded as a valuable model to explore the molecular mechanism of high-altitude animal adaptation, including the extreme tolerance of starvation and cold stress, which is closely associated with specifically seasonal deposition and metabolism of subcutaneous adipose tissue. However, the key regulators for these processes remain largely unknown. Here, the yak adipogenesis model in vitro was established and liver kinase b1 (LKB1) was regarded as a key negative regulator involved in yak...

Institutional trust and prosocial behavior in China: an experimental approach

Yi Xu & Yichi Zhang
We present three studies to demonstrate the important role of institutional trust in promoting prosocial behavior in China. In study 1, we used a Chinese national survey and found that institutional trust significantly predicted people’s donation and volunteer activities. In study 2, we experimentally examined the effects of institutional trust in public goods games. Participants allocated more money to public accounts when a positive impression of institutions was implicitly activated than when a negative impression...

Institutional trust and prosocial behavior in China: an experimental approach

Yi Xu & Yichi Zhang
We present three studies to demonstrate the important role of institutional trust in promoting prosocial behavior in China. In study 1, we used a Chinese national survey and found that institutional trust significantly predicted people’s donation and volunteer activities. In study 2, we experimentally examined the effects of institutional trust in public goods games. Participants allocated more money to public accounts when a positive impression of institutions was implicitly activated than when a negative impression...

Hsa-miR-186-3p suppresses colon cancer progression by inhibiting KRT18/MAPK signaling pathway

Tian Xia, Zhiyong Zhang, Xin Zhang & Quanfu Li
This study aimed to determine the effect of miR-186-3p and KRT18 interaction on the biological behavior of colon cancer cells. A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels. In addition, Western blotting was used to detect the KRT18 protein levels. The functional connection between KRT18 and miR-186-3p was confirmed using a dual luciferase reporter assay. BrdU incorporation, MTT assay, and flow cytometry were...

Hsa-miR-186-3p suppresses colon cancer progression by inhibiting KRT18/MAPK signaling pathway

Tian Xia, Zhiyong Zhang, Xin Zhang & Quanfu Li
This study aimed to determine the effect of miR-186-3p and KRT18 interaction on the biological behavior of colon cancer cells. A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels. In addition, Western blotting was used to detect the KRT18 protein levels. The functional connection between KRT18 and miR-186-3p was confirmed using a dual luciferase reporter assay. BrdU incorporation, MTT assay, and flow cytometry were...

Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication

Jinghua Yu, Wenyan Zhang, Wenbo Huo, Xiangling Meng, Ting Zhong, Ying Su, Yumeng Liu, Jinming Liu, Zengyan Wang, Fengmei Song, Shuxia Zhang, Zhaolong Li, Xiaoyan Yu, Xiaofang Yu & Shucheng Hua
Numerous viruses manipulate host factors for viral production. We demonstrated that human enterovirus A71 (EVA71), a primary causative agent for hand, foot, and mouth disease (HFMD), increased the level of the DNA damage response (DDR) marker γ-H2AX. DDR is primarily mediated by the ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), or DNA-dependent protein kinase (DNA-PK) pathways. Upregulation of γ-H2AX by EVA71 was dependent on the ATR but not the ATM or DNA-PK pathway. As...

Inflammatory activation and immune cell infiltration are main biological characteristics of SARS-CoV-2 infected myocardium

Rui Zhang, Xi Chen, Wenjie Zuo, Zhenjun Ji, Yangyang Qu, Yamin Su, Mingming Yang, Pengfei Zuo, Genshan Ma & Yongjun Li
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these...

Inflammatory activation and immune cell infiltration are main biological characteristics of SARS-CoV-2 infected myocardium

Rui Zhang, Xi Chen, Wenjie Zuo, Zhenjun Ji, Yangyang Qu, Yamin Su, Mingming Yang, Pengfei Zuo, Genshan Ma & Yongjun Li
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) can target cardiomyocytes (CMs) to directly invade the heart resulting in high mortality. This study aims to explore the biological characteristics of SARS-CoV-2 infected myocardium based on omics by collecting transcriptome data and analyzing them with a series of bioinformatics tools. Totally, 86 differentially expressed genes (DEGs) were discovered in SARS-CoV-2 infected CMs, and 15 miRNAs were discovered to target 60 genes. Functional enrichment analysis indicated that these...

Construction of an evenly-distributed genetic map using contig-tag-SNPs for quantitative trait loci (QTL) analysis of fiber-related traits in kenaf (Hibiscus cannabinus L.)

Yi Xu, Xuebei Wan, Siyuan Chen, Ting Liu, Jianmin Qi, Jiantang Xu & Liwu Zhang
Kenaf is one of the most important natural fiber crops worldwide, which aims at harvesting bast fiber. Mining QTL loci of fiber yield and quality traits will facilitate fiber improvement and molecular marker-assisted breeding in kenaf. In this study, Fuhong 952 and Zanyin No. 1 were used as parents to construct two mapping populations, F2 and F2:3, and an evenly distributed genetic linkage map was constructed by re-sequencing. The map contains 2512 contig-tag-SNP markers, and...

Correlation of the transcription factors IRF4 and BACH2 with the abnormal NFATC1 expression in T cells from chronic myeloid leukemia patients

Yikai Zhang, Xiangbo Zeng, Xianfeng Zha, Jing Lai, Guangxiao Tan, Shaohua Chen, Xibao Yu, Yangqiu Li & Ling Xu
T cell dysfunction is a common characteristic of patients with myeloid leukemia and is closely related to clinical efficacy and prognosis. In order to clarify the mechanisms leading to the T cell dysfunction, we characterized the gene expression profile of T cells from chronic myelogenous leukemia (CML) patients by microarray analysis and investigated the related regulating pathway. We employed gene expression profiling, bioinformatics and real-time quantitative reverse transcription PCR (RT-qPCR) to detect genes differentially expressed...

Outcomes and risk factors of hemorrhagic cystitis in pediatric allogeneic hematopoietic stem cell transplantation recipients using different graft source and condition with severe aplastic anemia

Bohan Li, Lijun Meng, Yuanyuan Tian, Qin Lu, Li Gao, Peifang Xiao, Jun Lu, Jie Li, Lin Wan, Zhiheng Li, Shaoyan Hu & Lingjun Kong
Hemorrhagic cystitis (HC) is a severe complication of allo-HSCT, characterized by irritative symptoms of the urinary tract and a higher morbidity rate. The risk factors and prognosis of HC are still unclear. The objective of this study is to identify risk factors and outcomes to improve treatment in pediatric SAA patients undergoing HSCTs in the Children's Hospital of Soochow University. A total of 97 SAA patients as a cohort were enrolled from 2010 to 2019...

Transcriptome-wide identification of WRKY transcription factors and their expression profiles in response to methyl jasmonate in Platycodon grandiflorus

Jing Li, Hanwen Yu, Mengli Liu, Bowen Chen, Nan Dong, Xiangwei Chang, Jutao Wang, Shihai Xing, Huasheng Peng, Liangping Zha & Shuangying Gui
Platycodon grandiflorus, a perennial flowering plant widely distributed in China and South Korea, is an excellent resource for both food and medicine. The main active compounds of P. grandiflorus are triterpenoid saponins. WRKY transcription factors (TFs) are among the largest gene families in plants and play an important role in regulating plant terpenoid accumulation, physiological metabolism, and stress response. Numerous studies have been reported on other medicinal plants; however, little is known about WRKY genes...

Repair mechanism of Wuwei Fuzheng Yijing formula in di-2-ethylhexyl phthalate-induced sperm DNA fragmentation in mice

Chenming Zhang, Shiqi Wang, Zulong Wang, Qi Zhang, Rubing Chen, Hao Zhang, Zhong Hua & Sicheng Ma
Di-2-ethylhexyl phthalate (DEHP), a known persistent organic pollutant, can increase the sperm DNA fragmentation index (DFI). To investigate the mechanism underlying the repair of DEHP-induced sperm DNA damage in mice by Wuwei Fuzheng Yijing (WFY) formula. The potential targets of WFY and sperm DNA fragment (SDF) were obtained from the TCMSP, BATMAN-TCM, OMIM and GeneCards. The protein–protein interaction (PPI) network, GO and KEGG pathway analyses of WFY-SDF were constructed. An animal model of DEHP-induced sperm...

Breed-specific expression mode of the Wnt signalling pathway is involved in feather follicle morphogenesis between Anser cygnoide and Anser anser

Ziqiang Feng, Haizhou Gong, Ichraf Mabrouk, Jinhong Fu, Chuanghang Li, Zebei Liu, Xu Tian, Le Sun, Keying Guo, Yujian Sui, Yuxuan Zhou, Yupu Song, Changguo Min, Jiangting Niu, Xiaomin Yan, Xiaohui Xu & Yongfeng Sun
Feathers are important economic products. Anser cygnoide (AC) is superior in reproduction, growth and feather quality, while Anser anser (AA) has an advantage in feather yield. Understanding the development of feather follicles facilitates deliberate crossbreeding. Here, we compared feather follicles morphogenesis between AC and AA. Feathers and dorsal skin were sampled at multiple embryonic days to investigate feather phenotypes, feather follicle morphology and the expression of hub molecules (FZD4, β-catenin, LEF1, TCF4 and c-Myc) in...

Additional file 2 of Assessing the causal association between human blood metabolites and the risk of epilepsy

Jiahao Cai, Xiaoyu Li, Shangbin Wu, Yang Tian, Yani Zhang, Zixin Wei, Zixiang Jin, Xiaojing Li, Xiong Chen & Wen-Xiong Chen
Additional file 2: Table S1. List of the identification (ID) for each of the 486 blood metabolites. Table S2. Harmonization data_482 blood metabolites and overall documented epilepsy from the ILAE consortium. Table S3. Results for the Mendelian randomization estimates and sensitivity analysis in epilepsy-subgroup (focal and generalized epilepsy) analysis. Table S4. Leave one out analysis for the causal association between blood metabolites and epilepsy subtypes.

Additional file 8 of ELOVL gene family plays a virtual role in response to breeding selection and lipid deposition in different tissues in chicken (Gallus gallus)

Dandan Wang, Xinyan Li, Panpan Zhang, Yuzhu Cao, Ke Zhang, Panpan Qin, Yulong Guo, Zhuanjian Li, Yadong Tian, Xiangtao Kang, Xiaojun Liu & Hong Li
Additional file 8: Table S6. All genomic SNPs in gene body and 2 kb promoter of ELOVL gene family used for PCA analysis.

Deciphering the lncRNA and mRNA profiles of Min pig backfat after acute cold stress

Dongjie Zhang, Wentao Wang, Zhongqiu Li, Liang Wang & Di Liu
Cold stress can promote fat metabolism and reduce fat deposition. The present study compared differentially expressed (DE) lncRNAs and mRNAs in the subcutaneous back fat of Min pigs subjected to 24 h acute cold stress. Transcriptome sequencing was used to identify DE lncRNAs and mRNAs. A lncRNA mRNA co expression network was constructed using the confirmed lncRNAs. GO and KEGG analyses were performed to investigate the biological processes involved in the response to acute cold...

Additional file 1 of Identification of HMGB2 associated with proliferation, invasion and prognosis in lung adenocarcinoma via weighted gene co-expression network analysis

Xie Qiu, Wei Liu, Yifan Zheng, Kai Zeng, Hao Wang, Haijun Sun & Jianhua Dai
Additional file 1: Table S1. Clinical informations and IHC score of patients with LUAD.

Additional file 6 of Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Changchuan Ye, Yuting Yang, Xi Chen, Lijie Yang, Xia Hua, Mengjie Yang, Xiangfang Zeng & Shiyan Qiao
Additional file 6: Fig S6. Standard Plot for ALA measurement. x: the absorbance at 554 nm; y: ALA concentration of sample after diluted (mg/L).

Additional file 2 of Neomorphic DNA-binding enables tumor-specific therapeutic gene expression in fusion-addicted childhood sarcoma

Tilman L. B. Hölting, Florencia Cidre-Aranaz, Dana Matzek, Bastian Popper, Severin J. Jacobi, Cornelius M. Funk, Florian H. Geyer, Jing Li, Ignazio Piseddu, Bruno L. Cadilha, Stephan Ledderose, Jennifer Zwilling, Shunya Ohmura, David Anz, Annette Künkele, Frederick Klauschen, Thomas G. P. Grünewald & Maximilian M. L. Knott
Additional file 2: Additional Tables 1-5.

Additional file 4 of Lrpap1 deficiency leads to myopia through TGF-β-induced apoptosis in zebrafish

Shanshan Liu, Ting Chen, Binghao Chen, Yijun Liu, Xiaohe Lu & Jiali Li
Additional file 3. Supplementary Table S2. Hematoxylin-Eosin (HE) staining procedure.

Additional file 5 of Lrpap1 deficiency leads to myopia through TGF-β-induced apoptosis in zebrafish

Shanshan Liu, Ting Chen, Binghao Chen, Yijun Liu, Xiaohe Lu & Jiali Li
Additional file 4. Supplementary Table S3. Information of 165 apoptosis pathway related genes in GSEA analysis.

Additional file 2 of ELOVL gene family plays a virtual role in response to breeding selection and lipid deposition in different tissues in chicken (Gallus gallus)

Dandan Wang, Xinyan Li, Panpan Zhang, Yuzhu Cao, Ke Zhang, Panpan Qin, Yulong Guo, Zhuanjian Li, Yadong Tian, Xiangtao Kang, Xiaojun Liu & Hong Li
Additional file 2: Table S2. Effects of 14 candidate SNPs on growth, carcass and meat quality traits.

Additional file 10 of Genome-wide identification and characterization of lncRNAs in sunflower endosperm

Shuai Yu, Zhichao Zhang, Jing Li, Yanbin Zhu, Yanzhe Yin, Xiaoyu Zhang, Yuxin Dai, Ao Zhang, Cong Li, Yanshu Zhu, Jinjuan Fan, Yanye Ruan & Xiaomei Dong
Additional file 10: Table S5. The summary of lncRNAs exhibiting allele-specific expression in cultivated sunflower lines for edible fruit and oil identified in 12 DAP sunflower endosperm.

Registration Year

  • 2022
    382

Resource Types

  • Dataset
    382

Affiliations

  • Guangzhou Medical University
    382
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    248
  • Southern Medical University
    217
  • Sichuan University
    215
  • Huazhong University of Science and Technology
    190
  • Sun Yat-sen University
    187
  • Zhejiang University
    187
  • Fudan University
    182
  • Shanghai Jiao Tong University
    169
  • Chinese Academy of Sciences
    159