1,441 Works

Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284

Huimin Wang, Dan Wang, Qiong Wei, Chun Li, Chunyan Li & Jing Yang
Long non-coding RNAs (lncRNAs) have been widely studied and play crucial roles in cervical cancer (CC) progression. Here, we investigated the function and mechanism of lncRNA PGM5-AS1 action in CC cells. Using real-time quantitative polymerase chain reaction or western blotting, PGM5-AS1 and decorin (DCN) were downregulated in CC tissues and cells, whereas miR-4284 was upregulated. Luciferase assay, RNA pull-down assay, and western blotting showed that PGM5-AS1 could sponge miR-4284 to upregulate DCN expression in CC...

The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication

Peili Hou, Xuefeng Wang, Hongmei Wang, Tiecheng Wang, Zhangping Yu, Chunqing Xu, Yudong Zhao, Wenqi Wang, Yong Zhao, Fengyun Chu, Huasong Chang, Hongchao Zhu, Jiahui Lu, Fuzhen Zhang, Xue Liang, Xingyu Li, Song Wang, Yuwei Gao & Hongbin He
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is closely related to various cellular aspects associated with autophagy. However, how SARS-CoV-2 mediates the subversion of the macroautophagy/autophagy pathway remains largely unclear. In this study, we demonstrate that overexpression of the SARS-CoV-2 ORF7a protein activates LC3-II and leads to the accumulation of autophagosomes in multiple cell lines, while knockdown of the viral ORF7a gene via shRNAs targeting ORF7a sgRNA during SARS-CoV-2 infection decreased autophagy levels. Mechanistically,...

The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication

Peili Hou, Xuefeng Wang, Hongmei Wang, Tiecheng Wang, Zhangping Yu, Chunqing Xu, Yudong Zhao, Wenqi Wang, Yong Zhao, Fengyun Chu, Huasong Chang, Hongchao Zhu, Jiahui Lu, Fuzhen Zhang, Xue Liang, Xingyu Li, Song Wang, Yuwei Gao & Hongbin He
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is closely related to various cellular aspects associated with autophagy. However, how SARS-CoV-2 mediates the subversion of the macroautophagy/autophagy pathway remains largely unclear. In this study, we demonstrate that overexpression of the SARS-CoV-2 ORF7a protein activates LC3-II and leads to the accumulation of autophagosomes in multiple cell lines, while knockdown of the viral ORF7a gene via shRNAs targeting ORF7a sgRNA during SARS-CoV-2 infection decreased autophagy levels. Mechanistically,...

Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Underlying Heterosis in Kenaf (Hibiscus Cannabinus L.) Drought Tolerance

Dengjie Luo, Shan Cao, Zengqiang Li, Meiqiong Tang, Caijin Wang, Enerand Mackon, Zhen Huang, Jiao Pan, Xia Wu, Qijing Wu, Hui Zhang, Renxue Li, Xin Li, Ru Li & Peng Chen
Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop with significant heterosis and tolerance to abiotic stresses. However, the mechanism underlying heterosis in kenaf under drought conditions is completely unknown. In this study, F1 hybrids were remarkably tolerant to drought stress. Through methylation-sensitive amplification polymorphism (MSAP) analysis, we highlighted that total DNA methylation level under drought decreased by 11.2% in F1 and increased by 29.7% and 13.1% in parents CP079 and CP071, respectively. Unlike parents,...

EFFECT OF THE PROBLEM-BASED LEARNING METHOD ON STUDENT ELECTROENCEPHALOGRAMS AND MICROCIRCULATORY BLOOD PERFUSION IN THE TEACHING OF SPORTS PHYSIOLOGY

Xiaoli Liu, Huan Zhu, Shufeng Zhou, Yong Peng, Mei Yang, Fen Chen & Xiaoming Li
ABSTRACT Introduction Applying the problem-based learning (PBL) method to the teaching of sports physiology. Objective This study explored the mechanism of the PBL method to improve the interest and learning effectiveness of students. Methods Twenty male students at the Physical Education College of Hubei Minzu University were randomly divided into a PBL group (10) and a traditional teaching method group (TTM). During the test, the subjects in the TTM group sat quietly listening to the...

CTPS1 inhibition suppresses proliferation and migration in colorectal cancer cells

Fahong Wu, Yudong Mao, Tao Ma, Xiaoli Wang, Hangzhi Wei, Tianwei Wang, Jia Wang & Youcheng Zhang
Colorectal cancer (CRC) is now the third most prevalent tumor and one of the deadliest cancers worldwide, with an increasing prevalence every year. Therefore, we urgently need to understand the mechanisms regulating the progression of colorectal cancer and find potential diagnostic biomarkers. In this study, we performed an analysis using the TCGA and GEO databases to find a molecular biomarker for the diagnosis of CRC, namely CTPS1. The results of this analysis revealed that CTPS1...

Construction of a High-Density Genetic Linkage Map and QTL Analysis of Fiber Yield Traits in Kenaf (Hibiscus Cannabinus L.) via SLAF-seq

Meixia Chen, Yi Xu, Siyuan Chen, Chengkang Zhang, Hui Shi, Ting Liu, Jiantang Xu, Jianmin Qi & Liwu Zhang
High-density genetic maps are vital for quantitative trait loci (QTL) mapping, genome assembly, and marker-assisted selection (MAS) in plants. Meanwhile, kenaf (Hibiscus cannabinus L.) is an important crop that produces raw fiber to many industries. However, both high-density genetic map construction and QTL identification have been limited in this crop due to insufficient molecular markers. Here, we constructed a high-density genetic linkage map in kenaf via specific-locus amplified fragment sequencing (SLAF-seq). An F7:8 mapping population...

FROM-GLC Plus: toward near real-time and multi-resolution land cover mapping

Le Yu, Zhenrong Du, Runmin Dong, Juepeng Zheng, Ying Tu, Xin Chen, Pengyu Hao, Bo Zhong, Dailiang Peng, Jiyao Zhao, Xiyu Li, Jianyu Yang, Haohuan Fu, Guangwen Yang & Peng Gong
Global land cover has undergone extensive and rapid changes as a result of human activities and climate change. These changes have had a significant impact on biodiversity, the surface energy balance, and sustainable development. Global land cover data underpins research on the development of earth system models, resource management, and evaluation of the ecological environment. However, there are limitations in the classification detail, spatial resolution, and rapid change monitoring capability of global land cover change...

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis

Jingru Bai, Tong Liu, Bo Tu, Meng Yuan, Zhaoqi Shu, Minghe Fan, Sihan Huo, Yuyao Guo, Lina Wang, Hua Wang & Ying Zhao
Cancer-associated fibroblasts (CAFs) are considered one of the most critical stromal cells that interact with pancreatic ductal adenocarcinoma (PDAC) and promote tumor growth, metastasis, and treatment resistance. Previous studies illustrated macroautophagy/autophagy contributes to CAF activation during tumor progression. Here in our study, we found that autophagy deficiency in CAFs impedes CAF activation by inhibiting proline biosynthesis and collagen production. Furthermore, we uncovered that autophagy promotes proline biosynthesis through mitophagy-mediated regulation of NADK2 (NAD kinase 2,...

Pediatric Rehabilitation Therapy and Physiotherapy Education in China

Lijiao Meng, Qingchuan Wei, Raymond C. C. Tsang, Panxing Peng, Xiaofan Pang & Lin Yang
To explore the pediatric rehabilitation curriculum, clinical placement, faculty characteristics, facilitators and barriers to curriculum implementation, and satisfaction of graduates of entry-level programs of rehabilitation therapy and physiotherapy in China. Two online cross-sectional surveys were conducted. With stratified random sampling, faculty were contacted to provide information on pediatric rehabilitation education in Survey A. In Survey B, the satisfaction of 2019 graduates was collected. Fifty-three faculty members (response rate 96.4%) completed Survey A and 154 graduates...

Sevoflurane reduces lipopolysaccharide-induced apoptosis and pulmonary fibrosis in the RAW264.7 cells and mice models to ameliorate acute lung injury by eliminating oxidative damages

Fushuang Zheng, Xiuying Wu, Jin Zhang, Zhiling Fu & Yan Zhang
Sevoflurane is identified as an effective candidate drug for acute lung injury (ALI) treatment, but its curing effects and detailed mechanisms have not been fully disclosed. The present study was designed to resolve this academic issue. The ALI mice models were established, and Hematoxylin-eosin staining assay was performed to examine tissue morphologies. Cell viability was determined by CCK-8 assay, and Annexin V-FITC/PI double staining assay was used to examine cell apoptosis. The expression levels of...

Comparative metabolic profiling of root, leaf, fruit, and stem tissues of Panax notoginseng

Rui Shi, Bingjie Xiong, Shu He, Can Liu, Jiftah Ben-Asher, Abraham Rami Horowitz, Shu Wang & Xiahong He
Panax notoginseng is highly used in traditional Chinese medicine, and its root is valued for ginsenoside contents. However, evidence suggests that other plant parts such as fruit, leaves, and stem are also potential sources of bioactive compounds. Therefore, this study aimed at providing insight into the differential accumulation of metabolites in fruit, leaf, root, and stem tissues collected from P. notoginseng. A total of 808 metabolites from 11 major metabolite classes were identified. Furthermore, 32...

Additional file 3 of The effectiveness of case management for cancer patients: an umbrella review

Nina Wang, Jia Chen, Wenjun Chen, Zhengkun Shi, Huaping Yang, Peng Liu, Xiao Wei, Xiangling Dong, Chen Wang, Ling Mao & Xianhong Li
Additional file 3.

Additional file 2 of Mitochondrial phylogenomics of Acanthocephala: nucleotide alignments produce long-branch attraction artefacts

Jin-Wei Gao, Xi-Ping Yuan, Hao Wu, Chuan-Yu Xiang, Min Xie, Rui Song, Zhong-Yuan Chen, Yuan-An Wu & Dong-Sheng Ou
Additional file 2: Dataset S1. Comparative overall mitogenomic architecture features of the Acanthocephala. Dataset S2. Comparative features of individual genes in mitogenomes of the Acanthocephala.

Probabilistic seismic performance assessment of offshore jacket structures as a basis for engineering decision-making

Samaneh Dashti, Karim Tarbali, Cong Zhou & James Geoffrey Chase
Jacket structures play an ever-increasing role in meeting the world’s burgeoning energy demand by providing support structure for exploiting offshore wind energy and oil and gas resources. Many of these structures are past their design lifetimes, and thus may have suffered significant degradation, highlighting the importance of investigating their earthquake response in seismically active regions. This study investigates the seismic performance of a typical offshore jacket platform, denoted as baseline structure, as well as four...

Additional file 2 of Identification of pyroptosis-related genes and long non-coding RNAs signatures in osteosarcoma

Jian Zhang, Jianjian Deng, Rui Ding, Jinghong Yuan, Jiahao Liu, Xiaokun Zhao, Tianlong Wu, Jingyu Jia & Xigao Cheng
Additional file 2: Table S3. The 57 PRGs

Additional file 1 of Identification of molecular subtypes based on inflammatory response in lower-grade glioma

Wanzun Lin, Jing Gao, Haojiong Zhang, Li Chen, Xianxin Qiu, Qingting Huang, Jiyi Hu, Lin Kong & Jiade J. Lu
Additional file 1. The clinical information of LGG patients obtained from TCGA, CGGA, and GEO database.

Additional file 10 of TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway

Rong Li, Jing Zhang, Qiong Wang, Meng Cheng & Bin Lin
Additional file 10: Table S1. The sequences for all primers and siRNA.

Additional file 2 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 2: Table S2. The rumen microbiota and the presence of quorum sensing molecules.

Additional file 2 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 2: Table S2. The rumen microbiota and the presence of quorum sensing molecules.

Additional file 2 of Comparison of meiotic transcriptomes of three maize inbreds with different origins reveals differences in cell cycle and recombination

Nelson Garcia, Lu Yin, Stefanie Dukowic-Schulze, Claire Milsted, Penny M. A. Kianian, Shahryar Kianian, Wojciech P. Pawlowski & Changbin Chen
Additional file 2: Supplementary Table 1. Percent of aligned reads for the B73, Mo17, and CML228 meiocytes to B73 v5 reference, Mo17 v1 reference, and CML228 v1 reference. Supplementary Table 2. Genes that had a reads per kilobase million (RPKM) value greater than 2 and were present in B73, Mo17, and CML228 meiocytes aligned to each of their corresponsive genomes (the B73 v5 reference, Mo17 v1 reference, and the CML228 v1 reference). Values shown for...

Additional file 2 of A novel signature model based on mitochondrial-related genes for predicting survival of colon adenocarcinoma

Hongli Gao & Fei Xing
Additional file 2. Raw data. (ZIP 320499 kb)

Additional file 2 of A novel signature model based on mitochondrial-related genes for predicting survival of colon adenocarcinoma

Hongli Gao & Fei Xing
Additional file 2. Raw data. (ZIP 320499 kb)

Additional file 1 of The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes

Yang Yang, Yiqi Wu, Mengting Ji, Xiaoyin Rong, Yanwei Zhang, Shuai Yang, Chang Lu, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li & Guoqing Cao
Additional file 1.

Additional file 4 of The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes

Yang Yang, Yiqi Wu, Mengting Ji, Xiaoyin Rong, Yanwei Zhang, Shuai Yang, Chang Lu, Chunbo Cai, Pengfei Gao, Xiaohong Guo, Bugao Li & Guoqing Cao
Additional file 4.

Registration Year

  • 2022
    1,441

Resource Types

  • Dataset
    1,441

Affiliations

  • Sichuan University
    1,441
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    949
  • Zhejiang University
    800
  • Shanghai Jiao Tong University
    786
  • Fudan University
    782
  • Huazhong University of Science and Technology
    726
  • Capital Medical University
    647
  • Sun Yat-sen University
    631
  • Chinese Academy of Sciences
    619
  • West China Hospital of Sichuan University
    567