25 Works

Data from: Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa

Lin Li, Xiaodong Jiao, Ilaria D’Atri, Fumihito Ono, Ralph Nelson, Chi-Chao Chan, Naoki Nakaya, Zhiwei Ma, Yan Ma, Xiaoying Cai, Longhua Zhang, Siying Lin, Abdul Hameed, Barry A. Chioza, Holly Hardy, Gavin Arno, Sarah Hull, Muhammad Imran Khan, James Fasham, V. Gaurav Harlalka, Michel Michaelides, Anthony T. Moore, Zeynep Hande Coban Akdemir, Shalini Jhangiani, James R. Lupski … & Frans P. M. Cremers
We identified a homozygous missense alteration (c.75C>A, p.D25E) in CLCC1, encoding a presumptive intracellular chloride channel highly expressed in the retina, associated with autosomal recessive retinitis pigmentosa (arRP) in eight consanguineous families of Pakistani descent. The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. TALEN KO in zebrafish was lethal 11 days post...

Genetic variability, community structure, and horizontal transfer of endosymbionts among three Asia II‐Bemisia tabaci mitotypes in Pakistan

Jorge R. Paredes-Montero, Judith Brown, Muhammad Zia-Ur-Rehman, Usman Hameed, Muhammad Haider & HansWerner Herrmann
Endosymbionts associated with the whitefly Bemisia tabaci cryptic species are known to contribute to host fitness and environmental adaptation. The genetic diversity and population complexity were investigated for endosymbiont communities of B. tabaci occupying different micro-environments in Pakistan. Mitotypes of B. tabaci were identified by comparative sequence analysis of the mitochondria cytochrome oxidase I (mtCOI) gene sequence. Whitefly mitotypes belonged to the Asia II- 1, -5, and -7 mitotypes of the Asia II major clade....

Data from: Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites (Co@AgNPs) and their density-dependent antibacterial activity

Zakia Kanwal, Muhammad Akram Raza, Saira Riaz, Saher Manzoor, Asima Tayyeb, Imran Sajid & Shahzad Naseem
Magnetic cores loaded with metallic nanoparticles can be promising nano-carriers for successful drug delivery at infectious sites. We report fabrication, characteristic analysis and in vitro antibacterial performance of nanocomposites comprising of cobalt cores (Co-cores) functionalized with varied concentration of silver nanoparticles (AgNPs). A two-step polyol process synchronized with transmetallation reduction method was used. Co-cores were synthesized by cobalt acetate and decoration of AgNPs was carried out by silver acetate. The density of AgNPs was varied...

Ethylene glycol assisted 3D floral evolution of BiFeO3-based nanostructures with effective magneto-electric response

Shahid Atiq, Syed Kumail Abbas, Ghulam Muhammad Mustafa, Murtaza Saleem, Muhammad Sufyan, Saira Riaz & Shahzad Naseem
Controlled growth of nanostructures plays a vital role in tuning the physical and chemical properties of functional materials for advanced energy and memory storage devices. Herein, we synthesized hierarchical micro-sized flowers, built by the self-assembly of highly crystalline, 2-dimensional nanoplates of Co and Ni doped BiFeO3, using a simple ethylene glycol mediated solvothermal method. Pure BiFeO3 attained scattered one-dimensional nanorods-type morphology having diameter nearly 60 nm. Co-doping of Co and Ni at Fe-site in BiFeO3...

ITS and LSU sequences, phylogenetic trees

Rubab Khurshid, Arooj Naseer, Israr Ahmad & Abdual Nasir Khalid
Russula kashmiriana sp. nov (subg. Tenellula, sect. Laricinae) has been collected and described from Himalayan coniferous forest of Azad Jammu and Kashmir (AJ&K), Pakistan. The taxon is characterized by bright reddish orange pileus with cracked margins and obvious reddish orange circle on the pileus, yellow context and light olive to yellowish amyloid basidiospores. Micro and macroscopic features along with molecular phylogenetic analysis based on large subunit (LSU) and internal transcribed spacer (ITS) sequences data confirmed...

Lepiota brunneogranulosa (Agaricaceae): a new species from Punjab, Pakistan, based on integrated taxonomy

Muhammad Asif, Aiman Izhar, Muhammad Haqnawaz, Abdul Rehman Niazi & Abdul Nasir Khalid
ABSTRACT During our surveys of mushrooms in 2019 and 2020, an interesting species of the genus Lepiota was collected from three different areas of Punjab (Bahawalnagar, Sheikhupura, and Muzaffarghar), Pakistan. These areas vary in their altitude and somewhat climatic conditions, nevertheless, morpho-anatomical comparisons and molecular analyses proved that these collections represented the same taxon, described here as Lepiota brunneogranulosa. This new species is characterized by a pileus with a yellowish-brown central disc, with brown colored...

Exploring phenotypic diversity of pigmented traits and iris features in Pakistani population

Saliha Bashir, Muhammad Shafique, Muhammad Shahzad, Muhammad Sohail Anjum & Ahmad Ali Shahid
Phenotypic variations of eye color, skin color, and iris surface features have been well-explored in certain populations. However, there has been comparatively little research on variations in these features in Pakistani population. The aim of this study is to discover phenotypic diversity and correlations of pigmented traits and iris surface features in Punjab and Khyber-Pakhtunkhwa (KPK) province of Pakistan. Digital images of eyes and skin were examined by investigators to determine color using Fitzpatrick Phototype...

Veloporphyrellus latisporus, a new and noteworthy species from moist temperate forests of Pakistan

Junaid Khan, Sadiq Ullah, Hassan Sher, Muhammad Fiaz & Abdul Khalid
Veloporphyrellus latisporus is proposed as a new species based on morphological characters and supporting molecular data from ITS and LSU regions of the nuclear DNA. The new species is characterized by its red to reddish brown granulose pileus and comparatively broader basidiospores. A key to the species of Veloporphyrellus is provided. This is the first report of any Veloporphyrellus species from Pakistan.

The genetic drivers for the successful invasive potential of a generalist bird, the House crow

Farheena Iqbal
What kind of genetic structure helps the rapid range expansion of the invasive species is fundamental to understand spread of invasion. The House crow (Corvus splendens), an ecological generalist, having a large native and introduced distribution range, is a good study model to investigate the genetic structure and adaptations underpinning the evolutionary potential for successful biological invasion. Thirteen mainland and one island native population from the Indian subcontinent were studied using four nuclear introns and...

Triple-combination therapy for cutaneous leishmaniasis using detergent-free, hyaluronate-coated elastic nanovesicles - Supplementary data set

Kashif Iqbal, Sidra Khalid, Craig A McElroy, Muhammad Adnan, Gul Majid Khan & M Junaid Dar
Aim: To develop and evaluate detergent-free, triple-drug-loaded, hyaluronate-coated elastic nanovesicles (H-ENVs) for the topical treatment of cutaneous leishmaniasis. Materials & methods: H-ENVs were developed and evaluated for vesicle size, entrapment efficiency, skin permeation and antileishmanial potential. Results: A 15.7 and 28.6% decrease in the cytotoxicity of paromomycin and amphotericin B, respectively, was observed in detergent-free ENVs compared with conventional ENVs. H-ENVs improved the efficacy of paromomycin against promastigote and amastigote models of leishmaniasis by 4-...

Time and magnitude monitoring using Weibull based Max-EWMA chart

Ayesha Talib, Sajid Ali, Ismail Shah & Farhana Gul
To monitor time and magnitude, different types of control charts have been proposed in the literature. This article proposes a new maximum exponentially weighted moving average (Max-EWMA) control chart by assuming Weibull distribution. The test-statistic of the Max-EWMA chart consists of two EWMA statistics, where one statistic is used for monitoring magnitude while the other to monitor time interval between events. The performances of the proposed chart is assessed on the basis of run length...

Lepiota brunneogranulosa (Agaricaceae): a new species from Punjab, Pakistan, based on integrated taxonomy

Muhammad Asif, Aiman Izhar, Muhammad Haqnawaz, Abdul Rehman Niazi & Abdul Nasir Khalid
ABSTRACT During our surveys of mushrooms in 2019 and 2020, an interesting species of the genus Lepiota was collected from three different areas of Punjab (Bahawalnagar, Sheikhupura, and Muzaffarghar), Pakistan. These areas vary in their altitude and somewhat climatic conditions, nevertheless, morpho-anatomical comparisons and molecular analyses proved that these collections represented the same taxon, described here as Lepiota brunneogranulosa. This new species is characterized by a pileus with a yellowish-brown central disc, with brown colored...

Antibiotic susceptibility patterns of bacterial isolates of patients with upper respiratory tract infections

Kalim Ullah, Marvi Baloch, Fahad Saleem, Ayaz Ali Khan, Hamid Saeed & Muhammad Islam
Abstract To evaluate the antibiotic susceptibility patterns in URTIs reporting to tertiary hospitals of Lahore. A cross-sectional study employing 259 culture sensitivity reports obtained from tertiary care hospitals of Lahore. Using SPSS, descriptive statistics were used to estimate frequencies and percentages. In URTIs, S. aureus (5%) was the frequent gram-positive isolate followed by MRSA (1.5%) and MSSA (1.5%), while P. aeruginosa (15.8%) was the prevalent gram-negative isolate followed by Klebsiella (13.1%) and E. coli (6.9%)....

Triple-combination therapy for cutaneous leishmaniasis using detergent-free, hyaluronate-coated elastic nanovesicles - Supplementary data set (nnm-2022-0077)

Kashif Iqbal, Sidra Khalid, Craig A McElroy, Muhammad Adnan, Gul Majid Khan & M Junaid Dar
Aim: To develop and evaluate detergent-free, triple-drug-loaded, hyaluronate-coated elastic nanovesicles (H-ENVs) for the topical treatment of cutaneous leishmaniasis. Materials & methods: H-ENVs were developed and evaluated for vesicle size, entrapment efficiency, skin permeation and antileishmanial potential. Results: A 15.7 and 28.6% decrease in the cytotoxicity of paromomycin and amphotericin B, respectively, was observed in detergent-free ENVs compared with conventional ENVs. H-ENVs improved the efficacy of paromomycin against promastigote and amastigote models of leishmaniasis by 4-...

Antibiotic susceptibility patterns of bacterial isolates of patients with upper respiratory tract infections

Kalim Ullah, Marvi Baloch, Fahad Saleem, Ayaz Ali Khan, Hamid Saeed & Muhammad Islam
Abstract To evaluate the antibiotic susceptibility patterns in URTIs reporting to tertiary hospitals of Lahore. A cross-sectional study employing 259 culture sensitivity reports obtained from tertiary care hospitals of Lahore. Using SPSS, descriptive statistics were used to estimate frequencies and percentages. In URTIs, S. aureus (5%) was the frequent gram-positive isolate followed by MRSA (1.5%) and MSSA (1.5%), while P. aeruginosa (15.8%) was the prevalent gram-negative isolate followed by Klebsiella (13.1%) and E. coli (6.9%)....

Cortinarius brunneocarpus var. microsporus, an interesting variety with Eurasian distribution

Junaid Khan, Hassan Sher, Shah Hussain & Abdul Nasir Khalid
Cortinarius brunneocarpus var. microsporus is described and illustrated from coniferous forests of District Swat, Pakistan based on morphological characters and phylogenetic analyses of the nuclear rDNA internal transcribed spacer (ITS) region. It is morphologically well-supported by significantly smaller basidiospores which are moderately to densely verrucose and with a suprahilar depression The new variety has Eurasian distribution and is being reported from Pakistan, Norway and Estonia. Differences between the new variety and similar taxa are briefly...

Biosynthesis, Characterization and Anti-dengue vector activity of Silver Nanoparticles prepared by Azadirachta indica and Citrullus colocynthis

Shafqat Rasool, Muhammad Akram Raza & Zakia Kanwal
Here, we report biosynthesis of silver nanoparticles (AgNPs) using aqueous extracts of (i) Azadirachta indica leaves and (ii) Citrullus colocynthis fruit and their larvicidal activity against Aedes aegypti. The Uv-Vis spectroscopy absorption peaks occurred in range 412-416 nm for A. indica AgNPs and 416-431nm for C. colocynthis AgNPs indicating the silver nature of prepared colloidal samples. The Scanning electron microscopy examination revealed the spherical morphology of both types of NPs with average size of 17±4...

Triple-combination therapy for cutaneous leishmaniasis using detergent-free, hyaluronate-coated elastic nanovesicles - Supplementary data set

Kashif Iqbal, Sidra Khalid, Craig A McElroy, Muhammad Adnan, Gul Majid Khan & M Junaid Dar
Aim: To develop and evaluate detergent-free, triple-drug-loaded, hyaluronate-coated elastic nanovesicles (H-ENVs) for the topical treatment of cutaneous leishmaniasis. Materials & methods: H-ENVs were developed and evaluated for vesicle size, entrapment efficiency, skin permeation and antileishmanial potential. Results: A 15.7 and 28.6% decrease in the cytotoxicity of paromomycin and amphotericin B, respectively, was observed in detergent-free ENVs compared with conventional ENVs. H-ENVs improved the efficacy of paromomycin against promastigote and amastigote models of leishmaniasis by 4-...

Glaciochemistry and Pigment Producing Ability of Bacteria from the Roof of the World, the Glaciers of Karakoram, Pakistan

Noor Hassan, Muhammad Rafiq, Abdul Haleem, Naveed Iqbal, Mohsin Khan, Aamir Ali Shah & Fariha Hasan
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ∼37% of its area glaciated. Glaciers harbor various microbes, including bacteria exposed to various extreme conditions. To thrive under these conditions, bacteria adopt various strategies, one of the most important being the production of pigments. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan. In addition,...

Glaciochemistry and Pigment Producing Ability of Bacteria from the Roof of the World, the Glaciers of Karakoram, Pakistan

Noor Hassan, Muhammad Rafiq, Abdul Haleem, Naveed Iqbal, Mohsin Khan, Aamir Ali Shah & Fariha Hasan
The Karakoram Mountain Range (KMR) is one of the largest mountain ranges in the world, with ∼37% of its area glaciated. Glaciers harbor various microbes, including bacteria exposed to various extreme conditions. To thrive under these conditions, bacteria adopt various strategies, one of the most important being the production of pigments. Here, we present the geochemistry of ice, sediment and meltwaters sampled from Ghulmet, Ghulkin and Hopar glaciers of the Karakoram Range, Pakistan. In addition,...

Time and magnitude monitoring using Weibull based Max-EWMA chart

Ayesha Talib, Sajid Ali, Ismail Shah & Farhana Gul
To monitor time and magnitude, different types of control charts have been proposed in the literature. This article proposes a new maximum exponentially weighted moving average (Max-EWMA) control chart by assuming Weibull distribution. The test-statistic of the Max-EWMA chart consists of two EWMA statistics, where one statistic is used for monitoring magnitude while the other to monitor time interval between events. The performances of the proposed chart is assessed on the basis of run length...

Data from: Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Marburg virus evolution

Izza Nasrullah, Azeem M Butt, Shifa Tahir, Muhammad Idrees & Yigang Tong
Background. The Marburg virus (MARV) has a negative-sense single-stranded RNA genome, belongs to the family Filoviridae, and is responsible for several outbreaks of highly fatal hemorrhagic fever. Codon usage patterns of viruses reflect a series of evolutionary changes that enable viruses to shape their survival rates and fitness toward the external environment and, most importantly, their hosts. To understand the evolution of MARV at the codon level, we report a comprehensive analysis of synonymous codon...

Impact of national drug pricing policy 2018 on access to medicines in Lahore Division, Pakistan: A pre-post survey study using WHO/HAI methodology

Amna Saeed, Yu Fang, Hamid Saeed, Zikria Saleem, Caijun Yang, Minghuan Jiang, Mingyue Zhao, Wenjing Ji, Muhammad Majid Aziz, Faiz Ullah Khan, Ali Hassan Gillani, Naveel Atif, Yu Fang & Zaheer-Ud-Din Babar
Objective: To evaluate the impact of new national drug pricing policy (NDPP) 2018 on the access to medicines in terms of prices, availability, and affordability. Design: Two cross-sectional surveys were undertaken before and after the launch of NDPP 2018, using a modified WHO/HAI methodology. Setting: Four districts of Lahore division, Pakistan. Participants: 16 public sector hospitals and 16 private sector retail pharmacies. Measures: The pre- and post-survey data on prices and availability of Lowest Price...

Chemical evidence of preserved collagen in a 54-million-year-old fish vertebrae

Suryendu Dutta, Sumit Kumar, Hukam Singh, Mahasin Khan, Amalan Barai, Anuradha Tewari, Rajendra Rana, Subir Bera, Shamik Sen & Ashoke Sahni
Collagens are the most abundant protein in the animal kingdom. Collagens form the structural framework of connective tissues such as bones, tendons and skin and play important biomechanical role in supporting tissue functions. The preservation of collagen in deep time is a topic of intense debate. Here we provide indisputable evidence of the presence of collagen in Early Eocene fish vertebrae using online pyrolysis-comprehensive two dimensional gas chromatography-time-of-flight mass spectrometry and immunofluorescence studies. The presence...

Morphometric analysis of the foramen magnum in sex determination: An additional 3DCT study from Nepal on a larger sample

Alok Atreya, Rijen Shrestha, Kiran Bhandari, Saurav Krishna Malla, Sumnima Acharya & Ritesh G Menezes
Background: Determination of sex of the skeletal remains plays a vital part in the identification of an individual. This study is focused on the morphometric measurement of the foramen magnum region and examining the accuracy of sexual dimorphism in the Nepalese population. Methods: Measurements were obtained from 3D computed tomography (CT) scan of 261 Nepalese adult cranial bases with known age and sex. Length and breadth of the foramen magnum, length and breadth of right...

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Affiliations

  • University of the Punjab
    25
  • University of Sargodha
    5
  • University of Education
    4
  • Hazara University
    3
  • Shenzhen University
    3
  • Guangxi University
    3
  • University of Swabi
    3
  • Quaid-i-Azam University
    3
  • University of the South Pacific
    2
  • University of Veterinary and Animal Sciences
    2