32 Works

Data from: The effectiveness of pseudomagic traits in promoting divergence and enhancing local adaptation

Maria R. Servedio & Bürger Reinhard
The first file contains the code necessary to generate figures in the style of Fig 1 of the associated manuscript. These figures show the divergence between both ecological traits and mating traits by the strength of mating preference. Different panels correspond to different rates of recombination between these loci that control these types of traits. Files S1 and S2 are Mathematica versions of the corresponding files in the Supplementary Information of the paper.

Do Synthesis Centers Synthesize? A Semantic Analysis of Topical Diversity in Research

Edward Hackett, Erin Leahy, John Parker, Ismael Rafols, Stephanie Hampton, Ugo Corte, Diego Chavarro, John Drake, Bart Penders, Laura Sheble, Niki Vermeulen & Todd Vision
Synthesis centers are a form of scientific organization that catalyzes and supports research that integrates diverse theories, methods and data across spatial or temporal scales to increase the generality, parsimony, applicability, or empirical soundness of scientific explanations. Synthesis working groups are a distinctive form of scientific collaboration that produce consequential, high-impact publications. But no one has asked if synthesis working groups synthesize: are their publications substantially more diverse than others, and if so, in what...

Code for: Same-sex sexual behaviour and selection for indiscriminate mating

Brian Lerch & Maria Servedio
File provides Mathematica code to replicate the analyses of "Same-sex sexual behaviour and selection for indiscriminate mating" by Lerch and Servedio. All figures from the manuscript (and its supplement) can be obtained by running the code. Comments for the code is provided for the "Additive death" section with other sections presented in parallel.

Data from: Genetic architecture influences when and how hybridization contributes to colonization

Bryan Reatini & Todd Vision
The role of genetic architecture in adaptation to novel environments has received considerable attention when the source of adaptation variation is de novo mutation. Relatively less is known when the source of adaptive variation is inter- or intraspecific hybridization. We model hybridization between divergent source populations and subsequent colonization of an unoccupied novel environment using individual-based simulations in order to understand the influence of genetic architecture on the timing of colonization and the mode of...

Supplemental materials for: Peri- and post-pubertal estrogen exposures of female mice optimize uterine responses later in life

Sylvia Hewitt, Marleny Carmona, Grace Foley, Lauren Donoghue, Sydney Lierz, Wipawee Winuthayanon & Kenneth Korach
At birth, all female mice, including those that either lack estrogen receptor α (ERα-knockout) or that express mutated forms of ERα (AF2ERKI), have a hypoplastic uterus. However, uterine growth and development that normally accompanies pubertal maturation does not occur in ERα-knockout or AF2ERKI mice, indicating ERα mediated estrogen signaling is essential for this process. Mice that lack Cyp19 (aromatase, ArKO mice), an enzyme critical for estrogen (E2) synthesis, are unable to make E2, and lack...

Interactive effects of previous and current thermal conditions on gene expression in Manduca sexta

Meggan A Alston, Jeeyun Lee, M. Elizabeth Moore, Joel G Kingsolver & Christopher S Willett
High temperatures can negatively impact performance and survival of organisms, particularly ectotherms. While an organism’s response to high temperature stress clearly depends on current thermal conditions, its response may also be affected by the temporal pattern and duration of past temperature exposures. We used RNA sequencing of Manduca sexta larvae fat body tissue to evaluate how diurnal temperature fluctuations during development affected gene expression both independently and in conjunction with subsequent heat stress. Additionally, we...

Carbon Dioxide (CO2) Fluxes from Terrestrial and Aquatic Environments in a High-Altitude Tropical Catchment

Diego Riveros-Iregui, Andrew Murray, Keridwen Whitmore, Chloe Schneider, Megan Raisle & Maribel Herrara

Genetic diversity and thermal performance in invasive and native populations of African fig flies

Aaron Comeault, Jeremy Wang, Silas Tittes, Kristin Isbell, Spencer Ingley, Allen Hurlbert & Daniel Matute
During biological invasions, invasive populations can suffer losses of genetic diversity that are predicted to negatively impact their fitness/performance. Despite examples of invasive populations harboring lower diversity than conspecific populations in their native range, few studies have linked this lower diversity to a decrease in fitness. Using genome sequences, we show that invasive populations of the African fig fly, Zaprionus indianus, have less genetic diversity than conspecific populations in their native range and that diversity...

Predicting the impact of patient and private provider behaviour on diagnostic delay for pulmonary tuberculosis patients in India: A simulation modelling approach

Sarang Deo, Simrita Singh, Neha Jha, Nimalan Arinaminpathy & Puneet Dewan
Background TB incidence in India continues to be high due, in large part, to long delays experienced by patients before successful diagnosis and treatment initiation, especially in the private sector. This diagnostic delay is driven by patients’ inclination to switch between different type of providers and providers’ inclination to delay ordering of accurate diagnostic tests relevant to TB. Our objective is to quantify the impact of changes in these behavioural characteristics of providers and patients...

Compensating for climate change-induced cue-environment mismatches: evidence for contemporary evolution of a photoperiodic reaction norm in Colias butterflies

Matthew Nielsen & Joel Kingsolver
Anthropogenic climate change alters seasonal conditions without altering photoperiod and can thus create a cue-environment mismatch for organisms that use photoperiod as a cue for seasonal plasticity. We investigated whether evolution of the photoperiodic reaction norm has compensated for this mismatch in Colias eurytheme. This butterfly’s wing melanization has a thermoregulatory function and changes seasonally. In 1971, Hoffmann quantified how larval photoperiod determines adult wing melanization. We recreated his experiment 47 years later using a...

Data for: Genetic architecture modulates diet induced hepatic mRNA and miRNA expression profiles

Excel Que, Kristen L. James, Alisha R. Coffey, Tangi L. Smallwood, Jody Albright, M. Nazmul Huda, Daniel Pomp, Praveen Sethupathy & Brian J. Bennett
Genetic approaches in model organisms have consistently demonstrated that molecular traits such as gene expression are under genetic regulation, similar to clinical traits. The resulting expression quantitative trait loci (eQTL) have revolutionized our understanding of genetic regulation and identified numerous candidate genes for clinically-relevant traits. More recently, these analyses have been extended to other molecular traits such as protein abundance, metabolite levels, and miRNA expression. Here we performed global hepatic eQTL and miRNA expression quantitative...

Data from: A stochastic model for predicting age and mass at maturity of insects

Geoffrey Legault & Joel Kingsolver
Variation in age and mass at maturity is commonly observed in populations, even among individuals with the same genetic and environmental backgrounds. Accounting for such individual variation with a stochastic model is important for estimating optimal evolutionary strategies and for understanding potential trade-offs among life history traits. However, most studies employ stochastic models that are either phenomenological or account for variation in only one life history trait. We propose a model based on the developmental...

Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia

Susan Dutcher, Ximena Bustamante-Marin, Amjad Horani, Mihaela Stoyanova, Wu-Lin Charng, Mathieu Bottier, Patrick Sears, Wei-Ning Yin, Leigh Anne Daniels, Hailey Bowen, Donald Conrad, Michael Knowles, Lawrence Ostrowski & Maimoona Zariwala
Primary ciliary dyskinesia (PCD) is characterized by chronic airway disease, reduced fertility, and randomization of the left/right body axis. It is caused by defects of motile cilia and sperm flagella. We screened a cohort of affected individuals that lack an obvious axonemal defect for pathogenic variants using whole exome capture, next generation sequencing, and bioinformatic analysis assuming an autosomal recessive trait. We identified one subject with an apparently homozygous nonsense variant [(c.1762C>T), p.(Arg588*)] in the...

Data on assessing the effects of genetic divergence and morphology on Anolis lizard mating

Emmanuel D'Agostino, Colin Donihue, Jonathan Losos & Anthony Geneva
The brown anole (Anolis sagrei) is a widespread neotropical lizard found on many islands in the West Indies as well as the coast of Central America. Across their range, brown anole populations show extensive ecomorphological trait variation and substantial genetic divergence. It is unclear, however, whether this genetic and morphological divergence results in reproductive isolation between populations. We investigated variation in mating behavior across populations by analyzing four hours of video of each of 234...

Data from: Differing thermal sensitivities in a host-parasitoid interaction: high, fluctuating developmental temperatures produce dead wasps and giant caterpillars

M. Elizabeth Moore, Christina A. Hill & Joel G. Kingsolver
1. Insect parasitoids, and the arthropod hosts they consume during development, are important ecological players in almost all environments across the globe. As ectothermic organisms, both parasitoid and host are strongly impacted by environmental temperature. If thermal tolerances differ between host insect and parasitoid, then the outcome of their interaction will be determined by the ambient temperature. As mean temperatures continue to rise and extreme temperatures become more frequent, we must determine the effect of...

Temperature-dependent competitive outcomes between the fruit flies Drosophila santomea and D. yakuba

Aaron Comeault & Daniel Matute
We use these data to test whether temperature can indirectly affect the fitness of Drosophila santomea and D. yakuba by altering interspecific competitive outcomes. We show that, when raised in isolation, both D. santomea and D. yakuba display similar variation in relative fitness across temperatures of 18°C, 22°C, and 25°C. However, D. santomea has higher fitness than D. yakuba when experiencing interspecific competition at 18°C, while the inverse is true at 25°C. Patterns of fitness...

Growth, stress and acclimation responses to fluctuating temperatures in field and domesticated populations of Manduca sexta

Joel Kingsolver, Joel Kingsolver, Megan Moore, Christina Hill & Kate Augustine
Diurnal fluctuations in temperature are ubiquitous in terrestrial environments, and insects and other ectotherms have evolved to tolerate or acclimate to such fluctuations. Few studies have examined whether ectotherms acclimate to diurnal temperature fluctuations, or how natural and domesticated populations differ in their responses to diurnal fluctuations. We examine how diurnally fluctuating temperatures during development affect growth, acclimation and stress responses for two populations of Manduca sexta: a field population that typically experiences wide variation...

Data from: An experimental investigation of how intraspecific competition and phenotypic plasticity can promote the evolution of novel, complex phenotypes

Nicholas Levis
Intraspecific competition has long been considered a key driver of evolutionary diversification, but whether it can also promote evolutionary innovation is less clear. We examined the interplay between competition and phenotypic plasticity in fueling the origins of a novel, complex phenotype––a distinctive carnivore morph found in spadefoot toad tadpoles (genus Spea) that specializes on fairy shrimp. We specifically sought to explore the possible origins of this phenotype by providing shrimp to Scaphiopus holbrookii tadpoles (the...

Paternally inherited P-element copy number affects the magnitude of hybrid dysgenesis in Drosophila simulans and D. melanogaster

Daniel Matute, Antonio Serrato-Capuchina, Jeremy Wang, Eric Earley, David Peede & Kristin Isbell
Transposable elements (TEs) are repetitive regions of DNA that are able to self-replicate and reinsert themselves throughout host genomes. Since the discovery of TEs, a prevalent question has been whether increasing TE copy number has an effect on the fitness of their hosts. P-elements (PEs) in Drosophila are a well-studied TE that has strong phenotypic effects. When a female without PEs (M) is crossed to a male with them (P), the resulting females are often...

How biomechanics, path-planning and sensing enable gliding flight in a natural environment

Tyson Hedrick & Pranav Khandelwal
Gliding animals traverse cluttered aerial environments when performing ecologically relevant behaviours. However, it is unknown how gliders execute collision-free flight over varying distances to reach their intended target. We quantified complete glide trajectories amid obstacles in a naturally behaving population of gliding lizards inhabiting a rainforest reserve. In this cluttered habitat, the lizards used glide paths with fewer obstacles than alternatives of similar distance. Their takeoff direction oriented them away from obstacles in their path...

Pot-1::mCherry transgene images from: Gametes deficient for Pot1 telomere binding proteins alter levels of telomeric foci for multiple generations

Evan Lister-Shimauchi
These images are raw data associated with the figures for the Communications Biolgoy manuscript "Gametes deficient for Pot1 telomere binding proteins alter levels of telomeric foci for multiple generations" (Lister-Shimauchi et. al., 2020). Subjects are C. elegans containing a pot-1::mCherry transgene. Some also contain a mNeonGreen::pot-2 transgene. Most subjects are early stage embryos.

Comparison of the costs of HPV testing through Community health campaigns versus Home-based testing in rural western Kenya: A micro-costing study

Easter Olwanda, James Khan, Yujung Choi, Jessica Islam & Megan Huchko
Objectives: To estimate the cost of HPV-based screening through Community health campaigns (CHCs) and home-based testing. Setting: Community health campaigns (CHCs) and home-based testing in six communities in rural western Kenya. Participants: CHCs and home-based screening reached 2297 and 1002 women aged 25 to 65 years respectively. Outcome measures: Outcome measures were overall cost per woman screened achieved through the CHCs and home-based testing, and the cost per woman for each activity comprising the screening...

Data from: Trait-based variation in host contribution to pathogen transmission across species and resource supplies

Miranda Welsh, James Cronin & Charles Mitchell
Two key knowledge gaps currently limit the development of more predictive and general models of pathogen transmission: (1) the physiological basis of heterogeneity in host contribution to pathogen transmission (reservoir potential) remains poorly understood, and (2) a general means of integrating the ecological dynamics of host communities has yet to emerge. If the traits responsible for differences in reservoir potential also modulate host community dynamics, these traits could be used to predict pathogen transmission as...

On the Books

Longitudinal white-matter abnormalities in sports-related concussion: a diffusion MRI study of the NCAA-DoD CARE Consortium

Yu-Chien Wu, Jaroslaw Harezlak, Nahla Elsaid, Zikai Lin, Qiuting Wen, Sourajit Mustafi, Larry Riggen, Kevin Koch, Andrew Nencka, Timothy Meier, Andrew Mayer, Yang Wang, Christopher Giza, John DiFiori, Kevin Guskiewicz, Jason Mihalik, Stephen LaConte, Stefan Duma, Steven Broglio, Andrew Saykin, Michael McCrea & Thomas McAllister
Objective To study longitudinal recovery trajectories of white-matter after sports-related concussion (SRC), we performed diffusion tensor imaging (DTI) on collegiate athletes who sustained SRC. Methods Collegiate athletes (n=219, 82 concussed athletes, 68 contact-sport controls, and 69 non-contact-sport controls) were included from the Concussion Assessment, Research and Education (CARE) Consortium. The participants completed clinical assessments and DTI at four time points: 24-48-hours post-injury, asymptomatic state, seven days following return-to-play, and six-months post-injury. Tract-based spatial statistics were...

Registration Year

  • 2020

Resource Types

  • Dataset


  • University of North Carolina
  • University of North Carolina at Chapel Hill
  • Bangor University
  • Duke University
  • Washington University in St. Louis
  • Washington State University
  • University of California, Davis
  • Northwestern University
  • Massachusetts General Hospital
  • Stanford University