20 Works

Human thymidylate synthase NMR relaxation data

Jeffrey Bonin, Andrew Lee & Paul Sapienza
Table of intensities of NMR signals of human thymidylate synthase in multiple bound forms from several NMR relaxation experiments. Bound forms studied include apo, dUMP (substrate) bound, TMP (product) bound, as well as apo and dUMP bound N-terminal truncation. Experiments include methyl 13C MQ and SQ CPMG, CHD2 methyl 13C CEST, CHD2 methyl 2H R2, solvent PRE, backbone amide RDC, and 15N relaxation. Details of the data collection can be found in the associated publication.

Parasites, niche modification and the host microbiome: A field survey of multiple parasites

Kayleigh R. O'Keeffe, Fletcher W. Halliday, Corbin D. Jones, Ignazio Carbone & Charles E. Mitchell
Parasites can affect and be affected by the host's microbiome, with consequences for host susceptibility, parasite transmission, and host and parasite fitness. Yet, two aspects of the relationship between parasite infection and host microbiota remain little understood: the nature of the relationship under field conditions, and how the relationship varies among parasites. To overcome these limitations, we performed a field survey of the within-leaf fungal community in a tall fescue population. We investigated how diversity...

Post-ACL reconstruction surgery rehabilitation dataset using isokinetic dynamometer and wearable IMUs

Robert Gutierrez, Joe Hart & Mehdi Boukhechba
Rehabilitation post-ACL reconstruction surgery is a lengthy process that involves a variety of exercises, with the goal of achieving leg symmetry. Testing for leg symmetry generally involves a series of tests, which may include walking gait analysis, isokinetic dynamometry, and single leg hop, if able. Isokinetic dynamometry readings have proven useful in understanding leg symmetry by providing a reading of muscle torque during a specific motion. However, the use of surface electromyography (sEMG) may provide...

Climate change alters sexual signaling in a desert-adapted frog

Gina Calabrese & Karin Pfennig
Climate change is altering species’ habitats, phenology, and behavior. Although sexual behaviors impact population persistence and fitness, climate change’s effects on sexual signals are understudied. Climate change can directly alter temperature-dependent sexual signals, cause changes in body size or condition that affect signal production, or alter the selective landscape of sexual signals. We tested whether temperature-dependent mating calls of Mexican spadefoot toads (Spea multiplicata) had changed in concert with climate in the Southwestern U.S.A. across...

Coastal squeeze on temperate reefs: long-term shifts in salinity, water quality, and oyster-associated communities

Maxwell Tice-Lewis, Y. Stacy Zhang, S. Gray Redding, Niels L. Lindquist, Claire M. Fieseler, Quentin A. Walker, Antonio B. Rodriguez & F. Joel Fodrie
Foundation species such as mangroves, saltmarshes, kelps, seagrasses, and oysters thrive within suitable environmental envelopes as narrow ribbons along the land-sea margin. Therefore, these habitat-forming species and resident fauna are sensitive to modified environmental gradients. For oysters, many estuaries impacted by sea-level rise, channelization, and municipal infrastructure are experiencing saltwater intrusion and water-quality degradation that may alter reef distributions, functions, and services. To explore decadal-scale oyster-reef community patterns across a temperate estuary in response to...

Human thymidylate synthase ITC data

Jeffrey Bonin, Andrew Lee & Paul Sapienza
This dataset includes isothermal titration calorimetry measurements of human thymidylate synthase (hTS) binding its product deoxythymidine monophosphate (TMP) and an N-terminal trunction of hTS binding its substrate deoxyuridine monophosphate (dUMP). These measurements include heats produced at various enzyme and ligand concentrations. Analysis of these data provide binding free energies, enthalpies, and entropies for these binding events. We find that in contrast to hTS binding its substrate dUMP, which we have previously shown to have positive...

Climatic niche conservatism in a clade of disease vectors (Diptera: Phlebotominae)

Emmanuel R. R. D'Agostino, Rafael Jose Vivero Gomez, Luis Romero, Eduar Bejarano, Allen Hurlbert, Aaron A. Comeault & Daniel Matute
Sandflies of the family Psychodidae show notable diversity in both disease vector status and climatic niche. Psychodid species’ ranges can be solely tropical, confined to the temperate zones, or span both. We obtained observation site data, and associated climate data, for 223 psychodid species to understand which aspects of climate most closely predict distribution. Temperature and seasonality are strong determinants of species occurrence within the clade. We built a mitochondrial DNA phylogeny of Psychodidae, and...

Volatile social environments can favour investments in quality over quantity of social relationships

Thomas G. Aubier & Hanna Kokko
Cooperation does not occur in a vacuum: interactions develop over time in social groups that undergo demographic changes. Intuition suggests that stable social environments favour developing few but strong reciprocal relationships (a 'focused' strategy), while volatile social environments favour the opposite: more but weaker social relationships (a 'diversifying' strategy). We model reciprocal investments under a quality-quantity tradeoff for social relationships. We find that volatility, counterintuitively, can favour a focused strategy. This result becomes explicable through...

Asymmetry of thermal sensitivity and the thermal risk of climate change

Lauren Buckley, Raymond Huey & Joel Kingsolver
Aim. Understanding and predicting the biological consequences of climate change requires considering the thermal sensitivity of organisms relative to environmental temperatures. One common approach involves “thermal safety margins” (TSMs), which are generally estimated as the temperature differential between the highest temperature an organism can tolerate (CTmax) and the mean or maximum environmental temperature it experiences. Yet, organisms face thermal stress and performance loss at body temperatures below their CTmax, and the steepness of that loss...

Can disease resistance evolve independently at different ages? Genetic variation in age-dependent resistance to disease in three wild plant species

Emily Bruns, Michael Hood, Janis Antonovics, Indigo Ballister, Sarah Troy & Jae Hoon Cho
1. Juveniles are typically less resistant (more susceptible) to infectious disease than adults, and this difference in susceptibility can help fuel the spread of pathogens in age-structured populations. However evolutionary explanations for this variation in resistance across age remain to be tested. 2. One hypothesis is that natural selection has optimized resistance to peak at ages where disease exposure is greatest. A central assumption of this hypothesis is that hosts have the capacity to evolve...

Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2

Wayne Stallaert, Taylor Enrico, Elizaveta Wick, Peter Ngoi, Michael Emanuele, Seth Rubin, Nicholas Brown, Jeremy Purvis, Taylor P Enrico, Elizaveta T Wick, Xianxi Wang, Seth M Rubin, Nicholas G Brown, Jeremy E Purvis & Michael J Emanuele
Cell cycle gene expression programs fuel proliferation and are universally dysregulated in cancer. The retinoblastoma (RB)-family of proteins, RB1, RBL1/p107, and RBL2/p130, coordinately represses cell cycle gene expression, inhibiting proliferation, and suppressing tumorigenesis. Phosphorylation of RB-family proteins by cyclin-dependent kinases is firmly established. Like phosphorylation, ubiquitination is essential to cell cycle control, and numerous proliferative regulators, tumor suppressors, and oncoproteins are ubiquitinated. However, little is known about the role of ubiquitin signaling in controlling RB-family...

The biogeography of community assembly: latitude and predation drive variation in community trait distribution in a guild of epifaunal crustaceans

Collin Gross, Collin Gross, J Duffy, Kevin Hovel, Melissa Kardish, Pamela Reynolds, Christoffer Boström, Katharyn Boyer, Mathiew Cusson, Johan Eklöf, Aschwin Engelen, Klemens Eriksson, Joel Fodrie, John Griffin, Clara Hereu, Masakazu Hori, A Randall Hughes, Mikhail Ivanov, Pablo Jorgensen, Claudia Kruschel, Kun-Seop Lee, Jonathan Lefcheck, Karen McGlathery, Per-Olav Moksnes, Masahiro Nakaoka … & Jay Stachowicz
While considerable evidence exists of biogeographic patterns in the intensity of species interactions, the influence of these patterns on variation in community structure is less clear. Using a model selection approach on measures of trait dispersion in crustaceans associated with eelgrass (Zostera marina) spanning 30º of latitude in two oceans, we found that dispersion strongly increased with increasing predation and decreasing latitude. Ocean and epiphyte load appeared as secondary predictors; Pacific communities were more overdispersed...

CeA Psilocin fiber photometry study: Data and custom code from experiments looking at changes in calcium dynamics in response to an air puff events

Devin Effinger
Psilocybin, and its active metabolite psilocin, have been shown to elicit rapid and long-lasting symptom improvements in a variety of affective psychiatric illnesses. However, the specific mechanisms behind these therapeutic effects remain relatively unknown. The central amygdala (CeA) is a primary output region within the extended amygdala that is heavily dysregulated in affective psychiatric disorders. Here, we utilized fiber photometry to measure changes in CeA reactivity to an aversive air puff stimulus after psilocin administration,...

Climate-driven thermal opportunities and risks for leaf miners in aspen canopies

H. Arthur Woods, Geoffrey Legault, Joel Kingsolver, Sylvain Pincebourde, Alisha Shah & Beau Larkin
In tree canopies, incoming solar radiation interacts with leaves and branches to generate temperature differences within and among leaves, presenting thermal opportunities and risks for leaf-dwelling ectotherms. Although leaf biophysics and insect thermal ecology are well understood, few studies have examined them together in single systems. We examined temperature variability in aspen canopies, Populus tremuloides, and its consequences for a common herbivore, the leaf-mining caterpillar Phyllocnistis populiella. We shaded leaves in the field and measured...

Hybridization alters the shape of the genotypic fitness landscape, increasing access to novel fitness peaks during adaptive radiation

Austin Patton, Emilie Richards, Katelyn Gould, Logan Buie & Christopher Martin
Estimating the complex relationship between fitness and genotype or phenotype (i.e. the adaptive landscape) is one of the central goals of evolutionary biology. However, adaptive walks connecting genotypes to organismal fitness, speciation, and novel ecological niches are still poorly understood and processes for surmounting fitness valleys remain controversial. One outstanding system for addressing these connections is a recent adaptive radiation of ecologically and morphologically novel pupfishes (a generalist, molluscivore, and scale-eater) endemic to San Salvador...

True or False IDO Data February 20, 2020 11.52

S.L. Eaddy, X. Lu, Y. Gin & L. Austin
COVID-19 measures have necessarily disrupted vaccinations and have been detrimental to measles prevention. As of October 2021, nearly 41 countries had paused their measles campaigns, leaving more than 94 million people at risk of missing vaccines. Therefore, it is critical for public health officials to continue to provide resources, promote vaccination, and change behaviors despite the pandemic. The study’s purpose is to enhance the information-environment level, population-level, and system-level outcomes to determine infectious disease outbreak...

Supplemental material for: The estrogen receptor α cistrome in human endometrium and epithelial organoids

Sylvia Hewitt, San-Pin Wu, Tianyuang Wang, Madhumita Ray, Marja Brolinson, Steven Young, Thomas Spencer, Alan DeCherney & Francsco DeMayo
Endometrial health is impacted by molecular processes that underlie estrogen responses. We assessed estrogen regulation of endometrial function by integrating the estrogen receptor alpha (ESR1) cistromes and transcriptomes of endometrial biopsies taken from the proliferative and mid-secretory phases of the menstrual cycle together with hormonally stimulated endometrial epithelial organoids. The cycle stage specific ESR1 binding sites were determined by ChIPseq and then integrated with changes in gene expression from RNAseq data to infer candidate ESR1...

Uncovering the effects of Müllerian mimicry on the evolution of conspicuousness in colour patterns

Ombeline Sculfort, Ludovic Maisonneuve, Marianne Elias, Thomas G. Aubier & Violaine Llaurens
Variation in the conspicuousness of colour patterns is observed within and among defended prey species. The evolution of conspicuous colour pattern in defended species can be strongly impaired because of increased detectability by predators. Nevertheless, such evolution of the colour pattern can be favoured if changes in conspicuousness result in Müllerian mimicry with other defended prey. Here, we develop a model describing the population dynamics of a conspicuous defended prey species, and we assess the...

Amazon forests capture high levels of atmospheric mercury pollution from artisanal gold mining

Jacqueline Gerson, Natalie Szponar, Arianna Agostini, Rand Alotaibi, Bridget Bergquist, Arabella Chen, Luis Fernandez, Kelsey Lansdale, Anne Lee, Maria Machicao, Melissa Marchese, Simon Topp, Claudia Vega & Emily Bernhardt
AbstractMercury emissions from artisanal and small-scale gold mining throughout the Global South exceed coal combustion as the largest global source of mercury. We examined mercury deposition and storage in an area of the Peruvian Amazon heavily impacted by artisanal gold mining. Intact forests in the Peruvian Amazon near gold mining receive extremely high inputs of mercury and experience elevated total mercury and methylmercury in the atmosphere, canopy foliage, and soils. Here we show for the...

Arabidopsis SAUR63 seedling phenotypes and fluorescent fusion protein localization

Jason Reed, Punita Nagpal & Brendan Trinidad
In plants, regulated cell expansion determines organ size and shape. Several members of the family of redundantly acting Small Auxin Up RNA (SAUR) proteins can stimulate plasma membrane (PM) H+-ATPase proton pumping activity by inhibiting PM-associated PP2C.D phosphatases, thereby increasing the PM electrochemical potential, acidifying the apoplast, and stimulating cell expansion. Similarly, Arabidopsis thaliana SAUR63 was able to increase growth of various organs, antagonize PP2C.D5 phosphatase, and increase H+-ATPase activity. This dataset includes i) measurements...

Registration Year

  • 2022

Resource Types

  • Dataset


  • University of North Carolina at Chapel Hill
  • University of North Carolina
  • University of Virginia
  • University of Washington
  • University of Zurich
  • University of British Columbia
  • College of Charleston
  • Bangor University
  • University of Montana
  • University of Pennsylvania