Data from: In vivo dynamics of skeletal muscle Dystrophin in zebrafish embryos revealed by improved FRAP analysis

Fernanda Bajanca, Vinicio Gonzalez-Perez, Sean J. Gillespie, Cyriaque Beley, Luis Garcia, Eric Theveneau, Richard P. Sear & Simon M. Hughes
Dystrophin forms an essential link between sarcolemma and cytoskeleton, perturbation of which causes muscular dystrophy. We analysed Dystrophin binding dynamics in vivo for the first time. Within maturing fibres of host zebrafish embryos, our analysis reveals a pool of diffusible Dystrophin and complexes bound at the fibre membrane. Combining modelling, an improved FRAP methodology and direct semi-quantitative analysis of bleaching suggests the existence of two membrane-bound Dystrophin populations with widely differing bound lifetimes: a stable,...

Registration Year

  • 2015
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  • Dataset
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Affiliations

  • University of Surrey
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  • Centre de Biologie du Développement
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  • King's College London
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  • Inserm
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  • Scientific Centre of Monaco
    1