Data from: An antibody screen of a Plasmodium vivax antigen library identifies novel merozoite proteins associated with clinical protectionCamila T. França, Jessica B. Hostetler, Sumana Sharma, Michael T. White, Enmoore Lin, Benson Kiniboro, Andreea Waltmann, Andrew W. Darcy, Connie S. Li Wai Suen, Peter Siba, Christopher L. King, Julian C. Rayner, Rick M. Fairhurst, Ivo Mueller & Connie S. N. Li Wai Suen
Background. Elimination of Plasmodium vivax malaria would be greatly facilitated by the development of an effective vaccine. A comprehensive and systematic characterization of antibodies to P. vivax antigens in exposed populations is useful in guiding rational vaccine design. Methodology/Principal Findings. In this study, we investigated antibodies to a large library of P. vivax entire ectodomain merozoite proteins in 2 Asia-Pacific populations, analysing the relationship of antibody levels with markers of current and cumulative malaria exposure,...
Plasmodium vivax is the geographically most widespread human malaria parasite. To analyze patterns of microsatellite diversity and population structure across countries of different transmission intensity, genotyping data from 11 microsatellite markers was either generated or compiled from 841 isolates from four continents collected in 1999–2008. Diversity was highest in South-East Asia (mean allelic richness 10.0–12.8), intermediate in the South Pacific (8.1–9.9) Madagascar and Sudan (7.9–8.4), and lowest in South America and Central Asia (5.5–7.2). A...
Data from: Severe childhood speech disorder: Gene discovery highlights transcriptional dysregulationMichael Hildebrand, Victoria Jackson, Thomas Scerri, Olivia Van Reyk, Matthew Coleman, Ruth Braden, Samantha Turner, Kristin Rigbye, Amber Boys, Sarah Barton, Richard Webster, Michael Fahey, Kerryn Saunders, Bronwyn Parry-Fielder, Georgia Paxton, Michael Hayman, David Coman, Himanshu Goel, Anne Baxter, Alan Ma, Noni Davis, Sheena Reilly, Martin Delatycki, Frederique Liégeois, Alan Connelly … & Angela Morgan
Objective: Determining the genetic basis of speech disorders provides insight into the neurobiology of human communication. Despite intensive investigation over the past two decades, the etiology of most children with speech disorder remains unexplained. Here we searched for a genetic etiology in children with severe speech disorder, specifically childhood apraxia of speech (CAS). Methods: Precise phenotyping together with research genome or exome analysis were performed on children referred with a primary diagnosis of CAS, as...
Data from: Variation in relapse frequency and the transmission potential of Plasmodium vivax malariaMichael T. White, George Shirreff, Stephan Karl, Azra C. Ghani & Ivo Mueller
There is substantial variation in the relapse frequency of Plasmodium vivax malaria, with fast relapsing strains in tropical areas, and slow relapsing strains in temperate areas with seasonal transmission. We hypothesise that much of the phenotypic diversity in P. vivax relapses arises from selection of relapse frequency to optimise transmission potential in a given environment, in a process similar to the virulence trade-off hypothesis. We develop mathematical models of P. vivax transmission and calculate the...
Data from: The temporal dynamics and infectiousness of subpatent Plasmodium falciparum infections in relation to parasite densityHannah C. Slater, Amanda Ross, Ingrid Felger, Natalie E. Hofmann, Leanne Robinson, Jackie Cook, Bronner P. Gonçalves, Anders Björkman, Andre Lin Ouedraogo, Ulrika Morris, Mwinyi Msellem, Cristian Koepfli, Ivo Mueller, Fitsum Tadesse, Endalamaw Gadisa, Smita Das, Gonzalo Domingo, Melissa Kapulu, Janet Midega, Seth Owusu-Agyei, Cécile Nabet, Renaud Piarroux, Ogobara Doumbo, Safiatou Niare Doumbo, Kwadwo Koram … & Lucy C. Okell
Malaria infections occurring below the limit of detection of standard diagnostics are common in all endemic settings. However, key questions remain surrounding their contribution to sustaining transmission and whether they need to be detected and targeted to achieve malaria elimination. In this study we analyse a range of malaria datasets to quantify the density, detectability, course of infection and infectiousness of subpatent infections. Asymptomatically infected individuals have lower parasite densities on average in low transmission...
Data from: Phylogeography of var gene repertoires reveals fine-scale geospatial clustering of Plasmodium falciparum populations in a highly endemic areaSofonias K. Tessema, Stephanie L. Monk, Mark B. Schultz, Livingstone Tavul, John C. Reeder, Peter M. Siba, Ivo Mueller & Alyssa E. Barry
Plasmodium falciparum malaria is a major global health problem that is being targeted for progressive elimination. Knowledge of local disease transmission patterns in endemic countries is critical to these elimination efforts. To investigate fine-scale patterns of malaria transmission, we have compared repertoires of rapidly evolving var genes in a highly endemic area. A total of 3680 high quality DBLα sequences were obtained from 68 P. falciparum isolates from ten villages spread over two distinct catchment...
Data from: The complex relationship of exposure to new Plasmodium infections and incidence of clinical malaria in Papua New GuineaNatalie E. Hofmann, Stephan Karl, Rahel Wampfler, Benson Kiniboro, Albina Teliki, Jonah Iga, Andreea Waltmann, Inoni Betuela, Ingrid Felger, Leanne J. Robinson & Ivo Mueller
The molecular force of blood-stage infection (molFOB) is a quantitative surrogate metric for malaria transmission at population level and for exposure at individual level. Relationships between molFOB, parasite prevalence and clinical incidence were assessed in a treatment-to-reinfection cohort, where P.vivax (Pv) hypnozoites were eliminated in half the children by primaquine (PQ). Discounting relapses, children acquired equal numbers of new P. falciparum (Pf) and Pv blood-stage infections/year (Pf-molFOB=0-18, Pv-molFOB=0-23) resulting in comparable spatial and temporal patterns...
Data from: Antibody responses to Plasmodium vivax Duffy binding and erythrocyte binding proteins predict risk of infection and are associated with protection from clinical malariaWen-Qiang He
Background. The Plasmodium vivax Duffy Binding Protein (PvDBP) is a key target of naturally acquired immunity. However, the functional receptor-binding region II of PvDBP (PvDBPII) is highly polymorphic. The natural acquisition of antibodies to different variants of PvDBPII, including AH, O, P and Sal1 alleles, the central region III-V, and P. vivax Erythrocyte Binding Protein region II (PvEBPII) and their associations with risk of clinical P. vivax malaria are not well understood. Methodology. Total IgG...
Data from: Blood-stage parasitaemia and age determine Plasmodium falciparum and P. vivax gametocytaemia in Papua New GuineaCristian Koepfli, Leanne J. Robinson, Patricia Rarau, Mary Salib, Naomi Sambale, Rahel Wampfler, Inoni Betuela, Wang Nuitragool, Alyssa E. Barry, Peter Siba, Ingrid Felger & Ivo Mueller
A better understanding of human-to-mosquito transmission is crucial to control malaria. In order to assess factors associated with gametocyte carriage, 2083 samples were collected in a cross-sectional survey in Papua New Guinea. Plasmodium species were detected by light microscopy and qPCR and gametocytes by detection of pfs25 and pvs25 mRNA transcripts by reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by PCR was 18.5% for Plasmodium falciparum and 13.0% for P. vivax. 52.5% of all infections were...
Data from: Strategies for understanding and reducing the Plasmodium vivax and Plasmodium ovale hypnozoite reservoir in Papua New Guinean children: a randomised placebo-controlled trial and mathematical modelLeanne J. Robinson, Rahel Wampfler, Inoni Betuela, Stephan Karl, Michael T. White, Connie S. N. Li Wai Suen, Natalie E. Hofmann, Benson Kiniboro, Andreea Waltmann, Jessica Brewster, Lina Lorry, Nandao Tarongka, Lornah Samol, Mariabeth Silkey, Quique Bassat, Peter M. Siba, Louis Schofield, Ingrid Felger, Ivo Mueller & Benson Kinboro
Background: The undetectable hypnozoite reservoir for relapsing Plasmodium vivax and P. ovale malarias presents a major challenge for malaria control and elimination in endemic countries. This study aims to directly determine the contribution of relapses to the burden of P. vivax and P. ovale infection, illness, and transmission in Papua New Guinean children. Methods and Findings: From 17 August 2009 to 20 May 2010, 524 children aged 5–10 y from East Sepik Province in Papua...
Data from: Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regionsRhea J. Longley, Michael T. White, Eizo Takashima, Masayuki Morita, Bernard N. Kanoi, Connie S. N. Li Wai Suen, Inoni Betuela, Andrea Kuehn, Piyarat Sripoorote, Camila T. Franca, Peter Siba, Leanne J. Robinson, Marcus Lacerda, Jetsumon Sattabongkot, Takafumi Tsuboi & Ivo Mueller
Plasmodium vivax remains an important cause of malaria in South America and the Asia-Pacific. Naturally acquired antibody responses against multiple P. vivax proteins have been described in numerous countries, however, direct comparison of these responses has been difficult with different methodologies employed. We measured antibody responses against 307 P. vivax proteins at the time of P. vivax infection, and at 2–3 later time-points in three countries. We observed that seropositivity rates at the time of...
Antibody features towards VAR2CSA and CSA binding infected erythrocytes in a cohort of pregnant women from PNGElizabeth Aitken, Amaya Ortega-Pajares, Agersew Alemu, Wina Hasang, Saber Dini, Holger Unger, Maria Ome-Kaius, Morten Nielsen, Ali Salanti, Joe Smith, Stephen Kent, P Mark Hogarth, Bruce Wines, Julie Simpson, Timon Damelang, Amy Chung & Stephen Rogerson
Plasmodium falciparum causes placental malaria, which results in adverse outcomes for mother and child. P. falciparum infected erythrocytes that express the parasite protein VAR2CSA on their surface can bind to placental chondroitin sulfate-A. It has been hypothesized that naturally acquired antibodies towards VAR2CSA protect against placental infection, but it has proven difficult to use measures of antibody to identify individuals protected from disease. We used a systems serology approach to identify naturally acquired antibody features...
Walter and Eliza Hall Institute of Medical Research12
University of Melbourne9
Papua New Guinea Institute of Medical Research5
University of Basel5
Swiss Tropical and Public Health Institute5
University of Barcelona3
University of Gezira1