Data from: Diversity and population structure of northern switchgrass as revealed through exome capture sequencingJoseph Evans, Emily Crisovan, Kerrie Barry, Chris Daum, Jerry Jenkins, Govindarajan Kunde-Ramamoorthy, Aruna Nandety, Chew Yee Ngan, Brieanne Vaillancourt, Chia-Lin Wei, Jeremy Schmutz, Shawn M. Kaeppler, Michael D. Casler, Carol Robin Buell & Chia-Lin Wei
Switchgrass (Panicum virgatum L.) is a polyploid, perennial grass species that is native to North America, and is being developed as a future biofuels feedstock crop. Switchgrass is present primarily in two ecotypes: a northern upland ecotype composed of tetraploid and octoploid accessions, and a southern lowland ecotype composed of primarily tetraploid accessions. We employed high-coverage exome capture sequencing (~2.4 Tb) to genotype 537 individuals from 45 upland and 21 lowland populations. From these data,...
Switchgrass metabolomics reveals striking genotypic and developmental differences in specialized metabolic phenotypesXingxing Li, Saurav J. Sarma, Lloyd W. Sumner, A. Daniel Jones & Robert L. Last
Switchgrass (Panicum virgatum L.) is a bioenergy crop that grows productively on lands not suitable for food production and is an excellent target for low-pesticide input biomass production. We hypothesize that resistance to insect pests and microbial pathogens is influenced by low-molecular-weight compounds known as specialized metabolites. We employed untargeted liquid chromatography–mass spectrometry, quantitative gas chromatography–mass spectrometry (GC–MS), and nuclear magnetic resonance spectroscopy to identify differences in switchgrass ecotype metabolomes. This analysis revealed striking differences...
Data from: Harvesting biofuel grasslands has mixed effects on natural enemy communities and no effects on biocontrol servicesTania N. Kim, Aaron F. Fox, Bill D. Wills, Timothy D. Meehan, Douglas A. Landis & Claudio Gratton
1.Perennial bioenergy systems, such as switchgrass and restored prairies, are alternatives to commonly used annual monocultures such as maize. Perennial systems require lower chemical input, provide greater ecosystem services such as carbon storage, greenhouse gas mitigation, and support greater biodiversity of beneficial insects. However, biomass harvest will be necessary in managing these perennial systems for bioenergy production, and it is unclear how repeated harvesting might affect ecosystem services. 2.In this study, we examined how repeated...
Data from: Cell wall composition and bioenergy potential of rice straw tissues are influenced by environment, tissue type, and genotypePaul Tanger, Miguel E. Vega-Sánchez, Margaret Fleming, Kim Tran, Seema Singh, James B. Abrahamson, Courtney E. Jahn, Nicholas Santoro, Elizabeth B. Naredo, Marietta Baraoidan, John M. C. Danku, David E. Salt, Kenneth L. McNally, Blake A. Simmons, Pamela C. Ronald, Hei Leung, Daniel R. Bush, John K. McKay & Jan E. Leach
Breeding has transformed wild plant species into modern crops, increasing the allocation of their photosynthetic assimilate into grain, fiber, and other products for human use. Despite progress in increasing the harvest index, much of the biomass of crop plants is not utilized. Potential uses for the large amounts of agricultural residues that accumulate are animal fodder or bioenergy, though these may not be economically viable without additional efforts such as targeted breeding or improved processing....
Data from: Generation of transcript assemblies and identification of single nucleotide polymorphisms from seven lowland and upland cultivars of switchgrassKevin L. Childs, Aruna Nandety, Candice N. Hirsch, Elsa Góngora-Castillo, Jeremy Schmutz, Shawn M. Kaeppler, Michael D. Casler & C. Robin Buell
Switchgrass (Panicum virgatum) is a North American perennial prairie species that has been used as a rangeland and forage crop and has recently been targeted as a potential biofuel feedstock species. Switchgrass, which occurs as tetraploid and octoploid forms, is classified into lowland or upland ecotypes that differ in growth phenotypes and adaptation to distinct habitats. Using RNA-sequencing reads derived from crown, young shoot and leaf tissues, we generated sequence data from seven switchgrass cultivars,...
Data from: Nucleotide polymorphism and copy number variant detection using exome capture and next generation sequencing in the polyploid grass Panicum virgatumJoseph Evans, Jeongwoon Kim, Kevin L. Childs, Brieanne Vaillancourt, Emily Crisovan, Aruna Nandety, Daniel J. Gerhardt, Todd A. Richmond, Jeffrey A. Jeddeloh, Shawn M. Kaeppler, Michael D. Casler & C. Robin Buell
Switchgrass (Panicum virgatum) is a polyploid, outcrossing grass species native to North America and has recently been recognized as a potential biofuel feedstock crop. Significant phenotypic variation including ploidy is present across the two primary ecotypes of switchgrass, referred to as upland and lowland switchgrass. The tetraploid switchgrass genome is approximately 1400 Mbp, split between two subgenomes, with significant repetitive sequence content limiting the efficiency of re-sequencing approaches for determining genome diversity. To characterize genetic...
Switchgrass (Panicum virgatum L.) is a perennial native North American grass present in two ecotypes: upland, found primarily in the northern range of switchgrass habitats, and lowland, found largely in the southern reaches of switchgrass habitats. Previous studies focused on a diversity panel of primarily northern switchgrass, so to expand our knowledge of genetic diversity in a broader set of North American switchgrass, exome capture sequence data were generated for 632 additional, primarily lowland individuals....
Great Lakes Bioenergy Research Center7
Michigan State University6
University of Wisconsin-Madison5
HudsonAlpha Institute for Biotechnology3
Joint Genome Institute3
University of Georgia1
Sandia National Laboratories1
University of Oklahoma1
Lawrence Berkeley National Laboratory1
University of Missouri1