Cronobacter sakazakii, an emerging opportunistic pathogen, is implicated in severe foodborne outbreak infections in premature and full-term infants. Generally, acid tolerance is vital for the pathogenesis of foodborne pathogens; however, its role in C. sakazakii virulence remains largely unknown. To screen out acid-tolerance determinants from transposon mutants, anovel counterselection method using gentamicin and acid was developed. Using the counterselection method and growth assay, we screened several acid-sensitive mutants and found that nlpD encodes an acid-resistance...

Fat deposition in skeletal muscle is an important aspect of improving meat quality. Isoflavones can promote animal anabolism, but whether and how they regulate muscle fat deposition remain largely unclear. In this study, we explored the role and corresponding molecular mechanism of one of the major isoflavones, daidzein, in fat deposition in C2C12 myoblast cells. In the absence of fatty acids (FAs), daidzein did not promote triglyceride synthesis and lipid droplet formation in cells but...

Dysfunction of macroautophagy/autophagy in macrophages contributes to atherosclerosis. Impaired autophagy-lysosomal degradation system leads to lipid accumulation, facilitating atherosclerotic plaque. ATG14 is an essential regulator for the fusion of autophagosomes with lysosomes. Whether ATG14 plays a role in macrophage autophagy dysfunction in atherosclerosis is unknown. To investigate the effects of ATG14 on macrophage autophagy, human atherosclerotic plaque, apoe−/- mice and cultured mouse macrophages were evaluated. Overexpression of ATG14 by adenovirus was used to reveal its function...

Dysfunction of macroautophagy/autophagy in macrophages contributes to atherosclerosis. Impaired autophagy-lysosomal degradation system leads to lipid accumulation, facilitating atherosclerotic plaque. ATG14 is an essential regulator for the fusion of autophagosomes with lysosomes. Whether ATG14 plays a role in macrophage autophagy dysfunction in atherosclerosis is unknown. To investigate the effects of ATG14 on macrophage autophagy, human atherosclerotic plaque, apoe−/- mice and cultured mouse macrophages were evaluated. Overexpression of ATG14 by adenovirus was used to reveal its function...

The African swine fever virus (ASFV) is a huge and complex DNA virus that can lead to the acute death of pigs and cause huge losses to the global swine industry. The CD2v protein is a transmembrane protein encoded by the ASFV’s EP402R gene, which can effectively inhibit the bystander lymphocyte proliferation in response to mitogens and mediate the absorption of red blood cells to ASFV-infected cells. The CD2v protein contains repetitive amino acid sequences...

Brucella endocarditis is a fatal complication and the most frequent cause of death for human brucellosis. This study aimed to systematically review the literature on the follow-up outcomes of Brucella endocarditis and analyze the determinants affecting the follow-up outcomes. The databases PubMed, Web of Science, Embase, and Cochrane were searched using keywords and suitable combinations. All studies reporting the follow-up outcomes of Brucella endocarditis were included. Finally, a total of 76 studies (207 patients), including...

Postoperative seroma is a known complication following spine deformity surgery. However, complete spinal cord injury (SCI) due to postoperative seroma is rare. Rehabilitation strategies and outcomes of SCI associated with postoperative seroma have been inadequately described. A 15-year-old female experienced inadvertent durotomy during pinal deformity correction surgery for idiopathic adolescent scoliosis. Despite immediate decompressive laminectomy, she developed complete loss of motor and sensory function with neurological level of injury at T10 immediately following the surgery....

Additional file 11: Table S10. Parameters of the disease-DEGs network.

Additional file 5: Table S4. Common differentially expressed genes of SS, NASH, and COVID-19 (n = 62).

Additional file 8: Table S7. PPI network parameters.

Additional file 1: Supplementary Table S1

Additional file 3: Supplementary Table S3

Additional file 6: Supplementary Table S6

Aim: Mouse models have been crucial to preclinical studies in the increasingly relevant fields of cell and gene therapy. However, only small quantities of mouse blood can be collected without producing adverse physiological effects that compromise data integrity. Results: To address this limitation, two combined methods were developed to create detailed droplet digital PCR (ddPCR) and hematological profiles using only ∼20 μl of mouse blood. The validation of these methods, which can serve as a...

Circular RNAs (circRNAs) are involved in multiple physiological processes. However, whether circRNAs function as the indicators of lung adenocarcinoma (LUAD) remains unclear. Three LUAD-related microarray datasets were downloaded from the Gene Expression Omnibus database, and overlapping differentially expressed circRNAs (DECs) in LUAD were identified. circ_0007618 and circ_0029426 were revealed to be significantly dysregulated in LUAD and verified in LUAD tissues and serum obtained in this study. Subsequently, the overall survival curve and receiver operating characteristics...

Additional file 2: Table S2. Cellular annotation and clinical characteristic of SMC and KUL3 cohort. Relate to Figure 1 and Figure S1.

Additional file 3: Table S3. Representative marker genes used for cell type annotation.

Additional file 6: Table S6. Identified meta-programs and its biological process annotation. Related to Figure 3F, Figure S2B.

Additional file 6: Table S6. Identified meta-programs and its biological process annotation. Related to Figure 3F, Figure S2B.

Additional file 7: Table S7. Key resource table.

Abstract A reinforced concrete (RC) column improved with central large-diameter butted steel rods (CLBSR column) has been proposed to increase the limit value of the axial compression ratio. This paper presents the seismic behavior analysis of CLBSR columns subjected to low-cycle reciprocating loading. The CLBSR columns were also compared with the RC column improved with central large-diameter steel rods (CLSR column) and the RC column improved with central reinforcement bars (CRB column) under the same...

Abstract A reinforced concrete (RC) column improved with central large-diameter butted steel rods (CLBSR column) has been proposed to increase the limit value of the axial compression ratio. This paper presents the seismic behavior analysis of CLBSR columns subjected to low-cycle reciprocating loading. The CLBSR columns were also compared with the RC column improved with central large-diameter steel rods (CLSR column) and the RC column improved with central reinforcement bars (CRB column) under the same...

Additional file 4: Table S4. The microbes and the presence of quorum sensing molecules in the pig gut (as the negative control group).

Additional file 8: Table S5. The diversity of predicted CahR-type receptors.

Additional file 8: Table S5. The diversity of predicted CahR-type receptors.