It has been well-documented that posttraumatic stress symptoms cause impairments in health-related quality of life (HRQoL). Until now we have little data on how DSM-5 PTSD symptom dimensions relate to different aspects of HRQoL. Clarifying this question would be informative to improve the quality of life of PTSD patients. This study aimed to investigate the effects of dimensions of a well-supported seven-factor model of DSM-5 PTSD symptoms on physical and psychosocial HRQoL. A total of...

Prostate cancer (PCa) is as a serious threat to male’s health around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) occupy an important position in various human cancers. However, the function and mechanism of lncRNA DNMBP antisense RNA 1 (DNMBP-AS1) in PCa is rarely investigated. RT-qPCR analysis was used to test gene expression. CCK-8, colony formation, EdU staining and transwell assays were conducted to assess the function of DNMBP-AS1 on PCa cell...

Abstract Chemotherapy is the main treatment option for advanced osteosarcoma, which is the most common type of primary bone malignancy. However, patients develop resistance rapidly and many succumb to the disease. Niclosamide, an anthelmintic drug, has been recently identified to display potent and selective anti-cancer activity. In this work, we show that niclosamide at sub-micromolar concentrations inhibits proliferation and migration, and induces apoptosis in both parental and chemo-resistant osteosarcoma cells, with much less toxicity in...

Existed methods like biochemical markers improve the accuracy of fluid evaluation for the maintenance hemodialysis patients, but none of them has become the gold standard. This study aimed to evaluate the potential of lung ultrasonography as a useful tool for monitoring the volume status of the patients. A total of 88 patients undergoing maintenance hemodialytic were enrolled in this prospective observational study. Patients were divided into three groups: overhydration (OH), normohydration, and hypohydration according to...

Oil Red O (ORO) staining is a commonly used experimental technique to detect lipid content in cells or tissues. Freshly prepared ORO in 60% isopropanol is the most widely used method at present. However, isopropanol is volatile and harmful to the human body. It will also affect the interpretation of the results due to the formation of crystals and non-specific diffuse staining. In this paper, by screening and validation, we report a salicylic acid ethanol...

We built a comprehensive dataset of the arboviruses and arthropod-specific viruses by curating worldwide available data from Arbovirus Catalog, Section VIII-F of the Biosafety in Microbiological and Biomedical Laboratories 6th edition, Virus Metadata Resource of International Committee on Taxonomy of Viruses, and GenBank. This dataset includes a complete information on viral taxonomy, biological characteristics, vectors and vertebrate hosts, distribution, recommended biosafety levels, genome segment, and nucleotide/amino acid sequences, which will facilitate research by scientists/researchers of...

The expression profiled from 340 distinct maize genotypes used for an eGWAS analysis. In the file named 'eGWAS340_repMerged_Untransformed_fpkm.csv' , FPKM of 19,565 genes profiled from the root tissues of 340 maize genotypes, replicated genotypes were averaged.

'eGWAS340_repUnmerged_Untransformed_fpkm.csv': FPKM of 19,565 genes profiled from the root tissues of 340 maize genotypes, replicated genotypes were kept raw for heritability calculation.
'eGWAS340_maf05het02.hmp.txt.gz': the SNP genotype data used for eQTL mapping and visible phenotype GWAS

Developing a confidence interval for the ratio of two quantities is an important task in statistics because of its omnipresence in real world applications. For such a problem, the MOVER-R (method of variance recovery for the ratio) technique, which is based on the recovery of variance estimates from confidence limits of the numerator and the denominator separately, was proposed as a useful and efficient approach. However, this method implicitly assumes that the confidence interval for...

Comparison of two survival curves is a fundamental problem in survival analysis. Although abundant frequentist methods have been developed for comparing survival functions, inference procedures from the Bayesian perspective are rather limited. In this article, we extract the quantity of interest from the classic log-rank test and propose its Bayesian counterpart. Monte Carlo methods, including a Gibbs sampler and a sequential importance sampling procedure, are developed to draw posterior samples of survival functions and a...

Abstract In this study, we investigated the effects of polymers on the pharmaceutical cocrystal formation process. Ibuprofen (IBU) was selected as the active pharmaceutical ingredient (API), nicotinamide (NIC) and saccharin (SAC) as the cocrystal coformer (CCF), ethanol/water as the solvent, polyvinylpyrrolidone (PVP) and poly (ethylene glycol) (PEG) as the representative polymers. We prepared IBU-NIC and IBU-SAC cocrystals in ethanol-water cosolvent in the absence or presence of polymers. Cocrystal screening products were characterized by FTIR, DSC,...

Abstract Based on the theory of single-phase elastic medium and unsaturated porous medium, the vibration isolation effect of composite multilayer wave impeding block (WIB) in the unsaturated ground under an underground dynamic load is investigated. The results show that the best vibration isolation effect can be obtained by designing the wave impedance ratio between the composite multilayer WIB and unsaturated ground. The composite multilayer WIB improves the vibration-damping bandwidth compared with the homogeneous WIB. The...

As a member of the tumor necrosis factor-α-induced protein 8 (TNFAIP8/TIPE) family, TIPE1 has been found to be associated with many cellular signaling pathways in regulating apoptosis, autophagy, and tumorigenesis. However, the position of TIPE1 in the signaling network remains elusive. Here we present the crystal structure of zebrafish TIPE1 in complex with phosphatidylethanolamine (PE) at a resolution of 1.38 Å. By comparison with structures of other three TIPE family proteins, a universal phospholipid-binding mode...

Motivated by a product warranty claims data set, we propose clustered coefficient regression models in a non-homogeneous Poisson process for recurrent event data. The proposed method, referred as CLUPP, can estimate the group structure and parameters simultaneously. In our proposed method, a penalized regression approach is used to identify the group structure. Numerical studies show that the proposed approach can identify the group structure well, and outperforms traditional methods such as hierarchical clustering and K-means....

Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and...

Abstract Objectives: To analyze and summarize the clinical features and image characteristics of Meniere’s Disease (MD) patients with Endolymphatic Hydrops (EH) confirmed by enhanced Magnetic Resonance Imaging (MRI). Methods: 252 MD patients with EH confirmed by MRI were enrolled. All patients met the diagnostic criteria forMD and underwent intravenous gadolinium injection. After 4 h, MR examinations were performed. The Nakashima grading standard was used to classify EH and evaluate its correlation with clinical features. Results:...

Supplementary Table 1. Neoantigens predicted and selected in this study. Supplementary Table 2. Significant expanded TCR clones specific to neoantigens

It is well known that non-small cell lung cancer (NSCLC) is a malignant tumor with high incidence in the world. We aimed to clarify a possible target and identify its precise molecular biological mechanism in NSCLC. NLR family CARD domain containing 5 (NLRC5) is widely expressed in tissues and exerts a vital role in anti-tumor immunity. We determined NLRC5 expression by RT-qPCR and western blot assay. The role of NLRC5 in the development of NSCLC...

Aggravated liver injury has been reported in aged ischemia/reperfusion-stressed livers; however, the mechanism of aged macrophage inflammatory regulation is not well understood. Here, we found that the adaptor protein TRIB1 plays a critical role in the differentiation of macrophages and the inflammatory response in the liver after ischemia/reperfusion injury. In the present study, we determined that aging promoted macrophage-mediated liver injury and that inflammation was mainly responsible for lower M2 polarization in liver transplantation-exposed humans...

Tumor-infiltrating lymphocytes (TILs) are vital elements of the tumor microenvironment (TME), and the anti-tumor activity of TILs on colorectal cancer (CRC) has been a topic of concern. However, the characteristics and prognosis of the various types of lymphocyte infiltration in CRC have not been fully explained. Our study aimed to identify distinct features and prognosis of TILs. We integrated multiple-cohort databases to illustrate the features, proportions, and prognosis of TILs on CRC. We found that...

This study aimed to elucidate the differences in vulval squamous cell carcinomas (VSCC) based on the HPV infection status. The sequencing data GSE183454 which contains 23 VSCC samples based on its HPV infection status was obtained from the Gene Expression Omnibus (GEO) database. We comprehensively dissected the differences of genomic and tumour microenvironment (TME) immune cell infiltration landscapes between HPV + and HPV- VSCC. The potential molecular mechanisms of prognostic genes were explored by functional...

The LncRNAs identified in Chinese sturgeon

Electron cyclotron harmonic (ECH) waves, which are responsible for the diffuse aurora, are usually observed with the strongest intensity in the first band. Here, we present observations of enhanced higher-band ECH with wave amplitudes ~1 mV/m by Van Allen Probes. Fully thermal simulation results indicate that these waves can be locally excited by the observed electron distributions, and higher plasma densities and lower magnetic strength are beneficial for exciting the higher-band ECH. The pitch angle...

Electron cyclotron harmonic (ECH) waves, which are responsible for the diffuse aurora, are usually observed with the strongest intensity in the first band. Here, we present observations of enhanced higher-band ECH with wave amplitudes ~1 mV/m by Van Allen Probes. Fully thermal simulation results indicate that these waves can be locally excited by the observed electron distributions, and higher plasma densities and lower magnetic strength are beneficial for exciting the higher-band ECH. The pitch angle...

The main purpose of the research on map emotional semantics is to describe and express the emotional responses caused by people observing images through computer technology. Nowadays, map application scenarios tend to be diversified, and the increasing demand for emotional information of map users bring new challenges for cartography. However, the lack of evaluation of emotions in the traditional map drawing process makes it difficult for the resulting maps to reach emotional resonance with map...

This study aimed to elucidate the differences in vulval squamous cell carcinomas (VSCC) based on the HPV infection status. The sequencing data GSE183454 which contains 23 VSCC samples based on its HPV infection status was obtained from the Gene Expression Omnibus (GEO) database. We comprehensively dissected the differences of genomic and tumour microenvironment (TME) immune cell infiltration landscapes between HPV + and HPV- VSCC. The potential molecular mechanisms of prognostic genes were explored by functional...