47 Works

A seven-gene signature and the C–C motif chemokine receptor family genes are the sarcoma-related immune genes

Jiarui Chen, Tuo Liang, Jiemei Cen, Jie Jiang, Shixin Pan, Shengsheng Huang, Liyi Chen, Xuhua Sun, Hao Li, Tianyou Chen, Wei Liang, Shian Liao, Chaojie Yu, Yuanlin Yao, Zhen Ye, Wuhua Chen, Hao Guo, Xinli Zhan & Chong Liu
Cells of the tumor microenvironment exert a vital influence on sarcoma prognosis. This study aimed to analyze and identify differentially expressed genes (DEGs) related to immunity and their significance as immune biomarkers for the accurate prediction of overall survival of patients with sarcoma. The Cancer Genome Atlas was adopted for obtaining sarcoma gene microarray and corresponding clinical information. ESTIMATE algorithm was used to calculate tumor immune microenvironment indices. Immune-associated DEGs were identified using the limma...

NLR family CARD domain containing 5 promotes hypoxia-induced cancer progress and carboplatin resistance by activating PI3K/AKT via carcinoembryonic antigen related cell adhesion molecule 1 in non-small cell lung cancer

Yu Dong, Tao Xu, Dongfan Li, Hua Guo, Xusheng Du, Guangshun Li, Jiakuan Chen, Bo Wang, Peng Wang, Gang Yu, Xuan Zhao & Ruiqi Xue
It is well known that non-small cell lung cancer (NSCLC) is a malignant tumor with high incidence in the world. We aimed to clarify a possible target and identify its precise molecular biological mechanism in NSCLC. NLR family CARD domain containing 5 (NLRC5) is widely expressed in tissues and exerts a vital role in anti-tumor immunity. We determined NLRC5 expression by RT-qPCR and western blot assay. The role of NLRC5 in the development of NSCLC...

sj-xlsx-2-jhs-10.1177_17531934221141986 - Supplemental material for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists

Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu & Qianqian Wang
Supplemental material, sj-xlsx-2-jhs-10.1177_17531934221141986 for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists by Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu and Qianqian Wang in Journal of Hand Surgery (European Volume)

sj-xlsx-4-jhs-10.1177_17531934221141986 - Supplemental material for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists

Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu & Qianqian Wang
Supplemental material, sj-xlsx-4-jhs-10.1177_17531934221141986 for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists by Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu and Qianqian Wang in Journal of Hand Surgery (European Volume)

MOVER-R and Penalized MOVER-R Confidence Intervals for the Ratio of Two Quantities

Peng Wang, Yilei Ma, Siqi Xu, Yi-Xin Wang, Yu Zhang, Xiangyang Lou, Ming Li, Baolin Wu, Guimin Gao, Ping Yin & Nianjun Liu
Developing a confidence interval for the ratio of two quantities is an important task in statistics because of its omnipresence in real world applications. For such a problem, the MOVER-R (method of variance recovery for the ratio) technique, which is based on the recovery of variance estimates from confidence limits of the numerator and the denominator separately, was proposed as a useful and efficient approach. However, this method implicitly assumes that the confidence interval for...

Physicochemical parameters of groundwater in coastal sandy aquifer

Zhenyan Wang, Qianqian Wang, Xuejing Wang, Yifan Guo, Yan Zhang, Kai Xiao, Shengchao Yu, Xiaolang Zhang, Chunmiao Zheng & Hailong Li
In the Yangjiang profile, a total of 29 (27 groundwater samples and 2 surface water samples: seawater and aquaculture wastewater) and 32 (29 groundwater samples and 2 surface water samples: seawater and aquaculture wastewater) samples were collected on August 12, 2020 (wet season) and November 8, 2020 (dry season), respectively. The measurement procedures for the trace metals (As, Ba, Cd, Cr, Fe, Mn, Pb, and Zn) in groundwater samples were described elsewhere (Luo et al.,...

QTL for yield per plant under water deficit and well-watered conditions and drought susceptibility index in soybean (Glycine max (L.) Merr.)

Zhangxiong Liu, Huihui Li, Xingrong Wang, Yanjun Zhang, Zuowang Gou, Xingzhen Zhao, Honglei Ren, Zixiang Wen, Yinghui Li, Lili Yu, Huawei Gao, Dechun Wang, Xusheng Qi & Lijuan Qiu
Drought has historically represented the greatest abiotic stress to adversely affect the stability of soybean (Glycine max (L.) Merr.) yields in non-irrigated field conditions. To investigate the genetic basis underlying drought tolerance in soybean, we screened the seed yield per plant (YP) in a natural diversity panel of 149 accessions under both water deficit (drought-stressed; YP-S) and well-watered (full irrigation; YP-W) conditions in field and greenhouse trials and calculated a drought susceptibility index (DSI) based...

Plant diversity data from modern sedimentary DNA of lakes in Siberia and China

Kathleen R. Stoof-Leichsenring, Sisi Liu, Weihan Jia, Kai Li, Luidmila A. Pestryakova, Steffen Mischke, Xianyong Cao, Xinqui Liu, Jian Ni, Stefan Neuhaus & Ulrike Herzschuh
Here we provide a large dataset on genetic plant diversity retrieved from surface sedimentary DNA (sedDNA) of lakes from Siberia and China spanning over a large environmental gradient. Our dataset encompasses sedDNA sequence data of 244 surface lake sediments and 3 soil samples originating from Siberia and Chinese lakes. We used a PCR-based metabarcoding approach combined with Next-Generation Sequencing to assess the modern and local plant diversity in and around the analysed lake localities. As...

A Gypsy element contributes to the nuclear retention and transcriptional regulation of the resident lncRNA in locusts

Xia Zhang, Ya′Nan Zhu, Bing Chen & Le Kang
The majority of long noncoding RNAs (lncRNAs) contain transposable elements (TEs). PAHAL, a nuclear-retained lncRNA that is inserted by a Gypsy retrotransposon, has been shown to be a vital regulator of phenylalanine hydroxylase (PAH) gene expression that controls dopamine biosynthesis and behavioural aggregation in the migratory locust. However, the role of the Gypsy retrotransposon in the transcriptional regulation of PAHAL remains unknown. Here, we identified a Gypsy retrotransposon (named Gypsy element) as an inverted long...

A seven-gene signature and the C–C motif chemokine receptor family genes are the sarcoma-related immune genes

Jiarui Chen, Tuo Liang, Jiemei Cen, Jie Jiang, Shixin Pan, Shengsheng Huang, Liyi Chen, Xuhua Sun, Hao Li, Tianyou Chen, Wei Liang, Shian Liao, Chaojie Yu, Yuanlin Yao, Zhen Ye, Wuhua Chen, Hao Guo, Xinli Zhan & Chong Liu
Cells of the tumor microenvironment exert a vital influence on sarcoma prognosis. This study aimed to analyze and identify differentially expressed genes (DEGs) related to immunity and their significance as immune biomarkers for the accurate prediction of overall survival of patients with sarcoma. The Cancer Genome Atlas was adopted for obtaining sarcoma gene microarray and corresponding clinical information. ESTIMATE algorithm was used to calculate tumor immune microenvironment indices. Immune-associated DEGs were identified using the limma...

Tire wear particles: An emerging threat to soil health

Jing Ding, Min Lv, Dong Zhu, Eva F. Leifheit, Qing-Lin Chen, Yun-Qing Wang, Ling-Xin Chen, Matthias C. Rillig & Yong-Guan Zhu
Tire wear particles (TWP) have caused widespread contamination in the environment. However, their behavior and potential impacts in soil remain unclear since studies have so far been mainly conducted in aquatic ecosystems. It is possible that soil ecosystems are already under pressure from pollution by TWP. We here firstly synthesize knowledge about the composition, distribution, and behavior of TWP in soils. By comparing with effects of microplastics (MP) in soil, we further outline several potential...

Additional file 4 of Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera

Xiao-Bin Fan, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Xin-Lin Gong & Chen-Zhu Wang
Additional file 4. Table S2. Summary of the chemicals used in our experiments.

Enhanced isohydric behavior decoupled the whole-tree sap flux response to leaf transpiration under nitrogen addition in a subtropical forest

Zhen Zhao, Ping Zhao, Zhenzhen Zhang, Ouyang Lei, Xiuhua Zhao, Liwei Zhu, Chenchen Cao & Linhui Zeng
Anthropogenic nitrogen deposition has the potential to change the leaf water-use strategy in the subtropical region of China. Nevertheless, the whole-tree level response crucial for ecosystem functions has not been well addressed over the past decades. In this study, the stem sap flux density (JS) was monitored for the whole-tree water transport capacity in two dominant species (Schima superba and Castanopsis chinensis) in a subtropical forest. To simulate the increased nitrogen deposition, the NH4NO3 solutions...

Assessing plant uptake of organic contaminants by food crops tomato, wheat, and corn through sap concentration factor

Majid Bagheri, Xiaolong He, Mariam K. Al-Lami, Nadege Oustriere, Wenyan Liu, Matt A. Limmer, Honglan Shi & Joel G. Burken
This study investigated uptake of two organic compounds including hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and exogenous caffeine by tomato (Solanum lycopersicum L.), corn (Zea mays L.), and wheat (Triticum aestivum L.). The plants were grown in a growth chamber under recommended conditions and then were exposed to these compounds for 19 days. The uptake of the compounds was measured by sap concentration factor. The plant samples (stem transpiration stream) and solution in the exposure media were taken and...

Assessing plant uptake of organic contaminants by food crops tomato, wheat, and corn through sap concentration factor

Majid Bagheri, Xiaolong He, Mariam K. Al-Lami, Nadege Oustriere, Wenyan Liu, Matt A. Limmer, Honglan Shi & Joel G. Burken
This study investigated uptake of two organic compounds including hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and exogenous caffeine by tomato (Solanum lycopersicum L.), corn (Zea mays L.), and wheat (Triticum aestivum L.). The plants were grown in a growth chamber under recommended conditions and then were exposed to these compounds for 19 days. The uptake of the compounds was measured by sap concentration factor. The plant samples (stem transpiration stream) and solution in the exposure media were taken and...

APPLICATION OF FUNCTIONAL TRAINING IN SOCCER FITNESS

BiHan Wang & Yu Zhang
ABSTRACT Introduction Although Chinese soccer has experienced many updates in its methods, there is still a large gap in players’ physical endurance compared to the world powers. Therefore, strengthening soccer players’ physical endurance through specific training methods is important in optimizing current performance. Objective Study the application of functional training in soccer players’ physical conditioning. Methods 20 junior soccer physical education student-athletes in colleges and universities were selected as the research object. The global functional...

Ropivacaine inhibits the proliferation and migration of colorectal cancer cells through ITGB1

Xiao Wang & Tianzuo Li
To study whether ropivacaine inhibits the proliferation and migration of colon cancer cells through ITGB1 (Integrin beta-1). First, the effect of ropivacaine on cell proliferation and migration was detected by MTT and Transwell. DAPI staining, annexin V staining and Western blot were used to detect the expression of apoptosis-related proteins to investigate the effect of ropivacaine on cell apoptosis. Using bioinformatics software to predict the potential drug targets of ropivacaine. RT-PCR, Western blot and immunofluorescence...

A Gypsy element contributes to the nuclear retention and transcriptional regulation of the resident lncRNA in locusts

Xia Zhang, Ya′Nan Zhu, Bing Chen & Le Kang
The majority of long noncoding RNAs (lncRNAs) contain transposable elements (TEs). PAHAL, a nuclear-retained lncRNA that is inserted by a Gypsy retrotransposon, has been shown to be a vital regulator of phenylalanine hydroxylase (PAH) gene expression that controls dopamine biosynthesis and behavioural aggregation in the migratory locust. However, the role of the Gypsy retrotransposon in the transcriptional regulation of PAHAL remains unknown. Here, we identified a Gypsy retrotransposon (named Gypsy element) as an inverted long...

Tire wear particles: An emerging threat to soil health

Jing Ding, Min Lv, Dong Zhu, Eva F. Leifheit, Qing-Lin Chen, Yun-Qing Wang, Ling-Xin Chen, Matthias C. Rillig & Yong-Guan Zhu
Tire wear particles (TWP) have caused widespread contamination in the environment. However, their behavior and potential impacts in soil remain unclear since studies have so far been mainly conducted in aquatic ecosystems. It is possible that soil ecosystems are already under pressure from pollution by TWP. We here firstly synthesize knowledge about the composition, distribution, and behavior of TWP in soils. By comparing with effects of microplastics (MP) in soil, we further outline several potential...

Tea seed meal application promoted microbial community diversity in the soil of peach orchard

Ping Sun, Jiansheng Shen, Xianrui Lin, Yu Zong, Yi Wang, Jianxi Zhu, Chenfei Chen, Yanqiao Wang, Huijuan Jia & Xinwei Wang
Soil microbes are indispensable in agricultural production, and they respond differently to different fertilization regimes. However, there is limited understating of microbial composition and function responses to an organic fertilizer tea seed meal (TSM) and conventional fertilizer. Here we first reported the change rules and functions of soil microbial community in peach orchard soil after application of compound fertilizer plus urea (CFU) and different amounts (1.25, 2.50, 3.75 and 5.00 kg) of TSM. Compared to...

sj-xlsx-1-jhs-10.1177_17531934221141986 - Supplemental material for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists

Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu & Qianqian Wang
Supplemental material, sj-xlsx-1-jhs-10.1177_17531934221141986 for Abnormal MRI signal intensity of the triangular fibrocartilage complex in asymptomatic wrists by Zhixin Wang, Shanlin Chen, Bo Liu, Zhanhua Qian, Jin Zhu and Qianqian Wang in Journal of Hand Surgery (European Volume)

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells

Zhimin Wang, Xiao Wang, Meiyu Bi, Xiao Hu, Qing Wang, Hao Liang & Dongjun Liu
As an important histone acetylase, the transcriptional coactivator P300/CBP affects target gene expression and plays a role in the maintenance of stem cell characteristics and differentiation potential. In this study, we explored the action of a highly effective selective histone acetylase inhibitor, C646, on goat adipose-derived stem cells (gADSCs), and investigated the impact of histone acetylation on the growth characteristics and the differentiation potential of ADSCs. We found that C646 blocked the cell proliferation, arrested...

Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells

Zhimin Wang, Xiao Wang, Meiyu Bi, Xiao Hu, Qing Wang, Hao Liang & Dongjun Liu
As an important histone acetylase, the transcriptional coactivator P300/CBP affects target gene expression and plays a role in the maintenance of stem cell characteristics and differentiation potential. In this study, we explored the action of a highly effective selective histone acetylase inhibitor, C646, on goat adipose-derived stem cells (gADSCs), and investigated the impact of histone acetylation on the growth characteristics and the differentiation potential of ADSCs. We found that C646 blocked the cell proliferation, arrested...

Additional file 2 of Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera

Xiao-Bin Fan, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Xin-Lin Gong & Chen-Zhu Wang
Additional file 2. Table S1. The TPM value of main olfactory related genes in Orco-/- and wild type.

Divergent responses of forest dominant trees species to the manipulated canopy and understory nitrogen additions in terms of foliage stoichiometric, economic and hydraulic traits

Zhenzhen Zhang, Ping Zhao, Lei Ouyang, Xiuhua Zhao, Liwei Zhu & Guangyan Ni
Nitrogen (N) deposition effects on the stoichiometric balance and photosynthetic and hydraulic couplings in subtropical forests has drawn wide attentions. The previously adopted understory application of N fertilization is criticized because it might ignore foliar N retention for different species. This paper reports a fertilizing application from the canopy (CAN) and under the canopy (UAN) in a phosphorus (P) limited ecosystem. Foliage stoichiometric, photosynthetic and hydraulic traits of six dominant species were measured and analyzed....

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