Data from: Blocking human contaminant DNA during PCR allows amplification of rare mammal species from sedimentary ancient DNA

Sanne Boessenkool, Laura S. Epp, James Haile, Eva Bellemain, Mary Edwards, Eric Coissac, Eske Willerslev & Christian Brochmann
Analyses of degraded DNA are typically hampered by contamination, especially when employing universal primers such as commonly used in environmental DNA studies. In addition to false-positive results, the amplification of contaminant DNA may cause false-negative results due to competition, or bias, during the PCR. In this study, we test the utility of human-specific blocking primers in mammal diversity analyses of ancient permafrost samples from Siberia. Using quantitative PCR (qPCR) on human and mammoth DNA we...

Registration Year

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  • Dataset
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Affiliations

  • University of Oslo
    26
  • Natural History Museum
    6
  • French National Centre for Scientific Research
    6
  • Lund University
    4
  • University of Gothenburg
    4
  • University of Cologne
    2
  • Oregon State University
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  • North-West University
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  • Moscow State University
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  • Lehigh University
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