4 Works
Investigating the role of RNA structures in transcriptional pausing using in vitro assays and in silico analyses
Simon Jeanneau, Pierre-Étienne Jacques & Daniel A. Lafontaine
Transcriptional pausing occurs across the bacterial genome but the importance of this mechanism is still poorly understood. Only few pauses were observed during the previous decades, leaving an important gap in understanding transcription mechanisms. Using the well-known Escherichia coli hisL and trpL pause sites as models, we describe here the relation of pause sites with upstream RNA structures suspected to stabilize pausing. We find that the transcription factor NusA influences the pause half-life at leuL,...
Time-course full profiling of circulating miRNAs in neurologically deceased organ donors: a proof of concept study to understand the onset of the cytokine storm
Andrée-Anne Clément, Daphnée Lamarche, Marie-Hélène Masse, Cécilia Légaré, Lee-Hwa Tai, Laurence Fleury Deland, Marie-Claude Battista, Luigi Bouchard & Frédérick D’Aragon
Neurologically deceased organ donors (NDDs) generally display an immune response involving an intense production of pro-inflammatory cytokines referred to as the cytokine storm. The sudden surge of inflammatory mediators in circulation promotes tissue and organ damages and ultimately leads to poor transplant outcome. As microRNAs (miRNAs) are frequently proposed as key regulators of inflammation and are relatively stable in circulation, changes in their profiles could play a role in the onset of the cytokine storm...
Time-course full profiling of circulating miRNAs in neurologically deceased organ donors: a proof of concept study to understand the onset of the cytokine storm
Andrée-Anne Clément, Daphnée Lamarche, Marie-Hélène Masse, Cécilia Légaré, Lee-Hwa Tai, Laurence Fleury Deland, Marie-Claude Battista, Luigi Bouchard & Frédérick D’Aragon
Neurologically deceased organ donors (NDDs) generally display an immune response involving an intense production of pro-inflammatory cytokines referred to as the cytokine storm. The sudden surge of inflammatory mediators in circulation promotes tissue and organ damages and ultimately leads to poor transplant outcome. As microRNAs (miRNAs) are frequently proposed as key regulators of inflammation and are relatively stable in circulation, changes in their profiles could play a role in the onset of the cytokine storm...
Investigating the role of RNA structures in transcriptional pausing using in vitro assays and in silico analyses
Simon Jeanneau, Pierre-Étienne Jacques & Daniel A. Lafontaine
Transcriptional pausing occurs across the bacterial genome but the importance of this mechanism is still poorly understood. Only few pauses were observed during the previous decades, leaving an important gap in understanding transcription mechanisms. Using the well-known Escherichia coli hisL and trpL pause sites as models, we describe here the relation of pause sites with upstream RNA structures suspected to stabilize pausing. We find that the transcription factor NusA influences the pause half-life at leuL,...