82 Works

Competing endogenous RNA network profiling reveals novel host dependency factors required for MERS-CoV propagation

Xi Zhang, Hin Chu, Lei Wen, Huiping Shuai, Dong Yang, Yixin Wang, Yuxin Hou, Zheng Zhu, Shuofeng Yuan, Feifei Yin, Jasper Fuk-Woo Chan & Kwok-Yung Yuen
Circular RNAs (circRNAs) are an integral component of the host competitive endogenous RNA (ceRNA) network. These noncoding RNAs are characterized by their unique splicing reactions to form covalently closed loop structures and play important RNA regulatory roles in cells. Recent studies showed that circRNA expressions were perturbed in viral infections and circRNAs might serve as potential antiviral targets. We investigated the host ceRNA network changes and biological relevance of circRNAs in human lung adenocarcinoma epithelial...

L-lysine potentiates aminoglycosides against Acinetobacter baumannii via regulation of proton motive force and antibiotics uptake

Wanyan Deng, Tiwei Fu, Zhen Zhang, Xiao Jiang, Jianping Xie, Hang Sun, Peng Hu, Hong Ren, Peifu Zhou, Qi Liu & Quanxin Long
Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is a leading cause of hospital- and community-acquired infections. Acinetobacter baumannii can rapidly acquire diverse resistance mechanisms and undergo genetic modifications that confer resistance and persistence to all currently used clinical antibiotics. In this study, we found exogenous L-lysine sensitizes Acinetobacter baumannii, other Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and a Gram-positive bacterium (Mycobacterium smegmatis) to aminoglycosides. Importantly, the combination of L-lysine with aminoglycosides killed clinically isolated multidrug-resistant...

The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia

Ying Zhou, Yu Tang, Pan Fu, Dongxing Tian, Lianhua Yu, Yunkun Huang, Gang Li, Meng Li, Yong Wang, Zehua Yang, Xiaogang Xu, Zhe Yin, Dongsheng Zhou, Laurent Poirel & Xiaofei Jiang
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids...

Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong

Ka Yi Kong, Thomas N. Y. Kwong, Hung Chan, Kristine Wong, Samuel S. Y. Wong, Anu P. Chaparala, Raphael C. Y. Chan, Lin Zhang, Joseph J. Y. Sung, Jun Yu, Peter M. Hawkey, Margaret Ip, William K. K. Wu & Sunny H. Wong
Clostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common C. difficile ribotype in Hong Kong that was associated with increased mortality. In this study, we assessed in vitro bacteriological characteristics and in vivo virulence of ribotype 002 compared to other common ribotypes, including ribotypes 012, 014 and 046. We observed significantly higher...

Risks and features of secondary infections in severe and critical ill COVID-19 patients

Haocheng Zhang, Yi Zhang, Jing Wu, Yang Li, Xian Zhou, Xin Li, Haili Chen, Mingquan Guo, Shu Chen, Feng Sun, Richeng Mao, Chao Qiu, Zhaoqin Zhu, Jingwen Ai & Wenhong Zhang
Objectives Severe or critical COVID-19 is associated with intensive care unit admission, increased secondary infection rate, and would lead to significant worsened prognosis. Risks and characteristics relating to secondary infections in severe COVID-19 have not been described. Methods Severe and critical COVID-19 patients from Shanghai were included. We collected lower respiratory, urine, catheters, and blood samples according to clinical necessity and culture and mNGS were performed. Clinical and laboratory data were archived. Results We found...

Development of an automatic integrated gene detection system for novel severe acute respiratory syndrome-related coronavirus (SARS-CoV2)

Yuchang Li, Jing Li, Ying Zhang, Lizhong Dai, Lin Li, Juan Liu, Sen Zhang, Xiaoyan Wu, Yi Hu, Chengfeng Qin, Tao Jiang & Xiaoping Kang
In December 2019, Wuhan, China suffered a serious outbreak of a novel coronavirus infectious disease (COVID) caused by novel severe acute respiratory syndrome-related coronavirus (SARS-CoV 2). To quickly identify the pathogen, we designed and screened primer sets, and established a sensitive and specific qRT-PCR assay for SARS-CoV 2; the lower limit of detection (LOD) was 15 (95% CI: 9.8–21) copies per reaction. We combined this qRT-PCR assay with an automatic integration system for nucleic acid...

Identification of novel tetracycline resistance gene tet(X14) and its co-occurrence with tet(X2) in a tigecycline-resistant and colistin-resistant Empedobacter stercoris

Yingying Cheng, Yong Chen, Yang Liu, Yuqi Guo, Yanzi Zhou, Tingting Xiao, Shuntian Zhang, Hao Xu, Yunbo Chen, Tongling Shan, Yonghong Xiao & Kai Zhou
Tigecycline is one of the last-resort antibiotics to treat severe infections. Recently, tigecycline resistance has sporadically emerged with an increasing trend, and Tet(X) family represents a new resistance mechanism of tigecycline. In this study, a novel chromosome-encoded tigecycline resistance gene, tet(X14), was identified in a tigecycline-resistant and colistin-resistant Empedobacter stercoris strain ES183 recovered from a pig fecal sample in China. Tet(X14) shows 67.14-96.39% sequence identity to the other variants [Tet(X) to Tet(X13)]. Overexpression of Tet(X14)...

The novel outer membrane protein from OprD/Occ family is associated with hypervirulence of carbapenem resistant Acinetobacter baumannii ST2/KL22

Mingxi Hua, Jingyuan Liu, Pengcheng Du, Xinzhe Liu, Min Li, Huizhu Wang, Chen Chen, Xinmin Xu, Yu Jiang, Yajie Wang, Hui Zeng & Ang Li
Acinetobacter baumannii has become a major healthcare threat that causes nosocomial infections, especially in critically ill patients. The spread of carbapenem-resistant A. baumannii (CRAB) strains has long been a clinical concern. It is important to study the epidemiology and virulence characteristics of different CRAB isolates in order to tailor infection prevention and antibiotic prescribing. In this study, a total of 71 CRAB isolates were collected in the hospital, and clinical characteristics of infections were analyzed....

HIV-1 resists MxB inhibition of viral Rev protein

Zhen Wang, Keli Chai, Qian Liu, Dong-Rong Yi, Qinghua Pan, Yu Huang, Juan Tan, Wentao Qiao, Fei Guo, Shan Cen & Chen Liang
The interferon-inducible myxovirus resistance B (MxB) protein has been reported to inhibit HIV-1 and herpesviruses by blocking the nuclear import of viral DNA. Here, we report a new antiviral mechanism in which MxB restricts the nuclear import of HIV-1 regulatory protein Rev, and as a result, diminishes Rev-dependent expression of HIV-1 Gag protein. Specifically, MxB disrupts the interaction of Rev with the nuclear transport receptor, transportin 1 (TNPO1). Supporting this, the TNPO1-independent Rev variants become...

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Chupeng Hu, Wei Liu, Ningyin Xu, An Huang, Zhenxing Zhang, Menghui Fan, Guojing Ruan, Yue Wang, Tao Xi & Yingying Xing
Tissue-resident memory T (TRM) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+TRM) cells in the gastric epithelium. However, the mechanistic details how CD4+TRM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened...

Defining the Syrian hamster as a highly susceptible preclinical model for SARS-CoV-2 infection

Kyle Rosenke, Kimberly Meade-White, Michael Letko, Chad Clancy, Frederick Hansen, Yanan Liu, Atsushi Okumura, Tsing-Lee Tang-Huau, Rong Li, Greg Saturday, Friederike Feldmann, Dana Scott, Zhongde Wang, Vincent Munster, Michael A. Jarvis & Heinz Feldmann
Following emergence in late 2019, SARS-CoV-2 rapidly became pandemic and is presently responsible for millions of infections and hundreds of thousands of deaths worldwide. There is currently no approved vaccine to halt the spread of SARS-CoV-2 and only very few treatment options are available to manage COVID-19 patients. For development of preclinical countermeasures, reliable and well-characterized small animal disease models will be of paramount importance. Here we show that intranasal inoculation of SARS-CoV-2 into Syrian...

A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang & Xinqiong Liu
The main aim of the work is to study the regulation of gene expression in the interaction between rice and Magnaporthe oryzae by gene chip technology. In this study, we mainly focused on changes of gene expression at 24, 48, and 72 hours post-inoculation (hpi), through which we could conduct a more comprehensive analysis of rice blast-related genes in the process of infection. The results showed that the experimental groups contained 460, 1227, and 3937...

A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang & Xinqiong Liu
The main aim of the work is to study the regulation of gene expression in the interaction between rice and Magnaporthe oryzae by gene chip technology. In this study, we mainly focused on changes of gene expression at 24, 48, and 72 hours post-inoculation (hpi), through which we could conduct a more comprehensive analysis of rice blast-related genes in the process of infection. The results showed that the experimental groups contained 460, 1227, and 3937...

sj-xlsx-1-tam-10.1177_1758835920977117 – Supplemental material for Low intratumor heterogeneity correlates with increased response to PD-1 blockade in renal cell carcinoma

Xia Ran, Jinyuan Xiao, Yi Zhang, Huajing Teng, Fang Cheng, Huiqian Chen, Kaifan Zhang & Zhongsheng Sun
Supplemental material, sj-xlsx-1-tam-10.1177_1758835920977117 for Low intratumor heterogeneity correlates with increased response to PD-1 blockade in renal cell carcinoma by Xia Ran, Jinyuan Xiao, Yi Zhang, Huajing Teng, Fang Cheng, Huiqian Chen, Kaifan Zhang and Zhongsheng Sun in Therapeutic Advances in Medical Oncology

DHX9 interacts with APOBEC3B and attenuates the anti-HBV effect of APOBEC3B

Yanmeng Chen, Bocun Shen, Xiaochuan Zheng, Quanxin Long, Jie Xia, Yao Huang, Xuefei Cai, Deqiang Wang, Juan Chen, Ni Tang, Ailong Huang & Yuan Hu
Hepatitis B virus (HBV) is a partially double-stranded DNA virus that replicates by reverse transcription. We previously demonstrated that the host restriction factor-APOBEC3B (A3B) inhibited HBV replication which was dependent on its deaminase activity during reverse transcription. However, the host factors involved in the process of regulating the anti-HBV function of A3B are less known. In this research, to obtain a comprehensive understanding of the interaction networks of A3B, we conducted coimmunoprecipitation and mass spectrometry...

Development of an automatic integrated gene detection system for novel severe acute respiratory syndrome-related coronavirus (SARS-CoV2)

Yuchang Li, Jing Li, Ying Zhang, Lizhong Dai, Lin Li, Juan Liu, Sen Zhang, Xiaoyan Wu, Yi Hu, Chengfeng Qin, Tao Jiang & Xiaoping Kang
In December 2019, Wuhan, China suffered a serious outbreak of a novel coronavirus infectious disease (COVID) caused by novel severe acute respiratory syndrome-related coronavirus (SARS-CoV 2). To quickly identify the pathogen, we designed and screened primer sets, and established a sensitive and specific qRT-PCR assay for SARS-CoV 2; the lower limit of detection (LOD) was 15 (95% CI: 9.8–21) copies per reaction. We combined this qRT-PCR assay with an automatic integration system for nucleic acid...

Burkholderia pseudomallei interferes with host lipid metabolism via NR1D2-mediated PNPLA2/ATGL suppression to block autophagy-dependent inhibition of infection

Mengling Tang, Zhiqiang Hu, Chenglong Rao, Jiangao Chen, Siqi Yuan, Jiangang Zhang, Chan Mao, Jingmin Yan, Yupei Xia, Meijuan Zhang, Juanjuan Yue, Yang Xiang, Jianping Xie, Xuhu Mao & Qian Li
Burkholderia pseudomallei: which causes melioidosis with high mortality in humans, has become a global public health concern. Recently, infection-driven lipid droplet accumulation has been related to the progression of host-pathogen interactions, and its contribution to the pathogenesis of infectious disease has been investigated. Here, we demonstrated that B. pseudomallei infection actively induced a time-dependent increase in the number and size of lipid droplets in human lung epithelial cells and macrophages. We also found that lipid...

Protective effect of brain-derived neurotrophic factor and neurotrophin-3 overexpression by adipose-derived stem cells combined with silk fibroin/chitosan scaffold in spinal cord injury

Wen-Chen Ji, Meng Li, Wan-Ting Jiang, Xing Ma & Jia Li
Objectives: Spinal cord injury (SCI) is a most debilitating traumatic injury, and cytotherapy is a promising alternative treatment strategy. Here we investigated the effect and mechanism of adipose-derived stem/stromal cells (ASCs) with overexpressing brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) (BDNF-NT3) in combination with silk fibroin/chitosan scaffold (SFCS) in SCI. Methods: Female Sprague-Dawley rats were used as an SCI model. SFCS,SFCS and ASCs, or ASCs overexpressing NT3, BDNF, and BDNF-NT3 were implanted into SCI rats....

Proscillaridin A slows the prostate cancer progression through triggering the activation of endoplasmic reticulum stress

Fan Wang, Lin Liu, Yu Tong, Linfeng Li, Yanfeng Liu & Wei-Qiang Gao
Prostate cancer (PCa) is the second commonly diagnosed malignancy in men over the world. Although androgen deprivation therapy for advanced PCa patients has significantly improved their survival, the majority of these patients eventually develop castration-resistant prostate cancer (CRPC). Proscillaridin A (Pro A), a cardiac glycoside that is clinically used to treat various heart failure diseases, has been reported to have anticancer activity in several cancers. However, whether Pro A exerts an inhibitory effect on PCa...

Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018

Yanqun Wang, Xin Li, Wenkuan Liu, Mian Gan, Lu Zhang, Jin Wang, Zhaoyong Zhang, Airu Zhu, Fang Li, Jing Sun, Guoxian Zhang, Zhen Zhuang, Jiaying Luo, Dehui Chen, Shuyan Qiu, Li Zhang, Duo Xu, Chris Ka Pun Mok, Fuchun Zhang, Jingxian Zhao, Rong Zhou & Jincun Zhao
Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and...

Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong

Ka Yi Kong, Thomas N. Y. Kwong, Hung Chan, Kristine Wong, Samuel S. Y. Wong, Anu P. Chaparala, Raphael C. Y. Chan, Lin Zhang, Joseph J. Y. Sung, Jun Yu, Peter M. Hawkey, Margaret Ip, William K. K. Wu & Sunny H. Wong
Clostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common C. difficile ribotype in Hong Kong that was associated with increased mortality. In this study, we assessed in vitro bacteriological characteristics and in vivo virulence of ribotype 002 compared to other common ribotypes, including ribotypes 012, 014 and 046. We observed significantly higher...

Defining the Syrian hamster as a highly susceptible preclinical model for SARS-CoV-2 infection

Kyle Rosenke, Kimberly Meade-White, Michael Letko, Chad Clancy, Frederick Hansen, Yanan Liu, Atsushi Okumura, Tsing-Lee Tang-Huau, Rong Li, Greg Saturday, Friederike Feldmann, Dana Scott, Zhongde Wang, Vincent Munster, Michael A. Jarvis & Heinz Feldmann
Following emergence in late 2019, SARS-CoV-2 rapidly became pandemic and is presently responsible for millions of infections and hundreds of thousands of deaths worldwide. There is currently no approved vaccine to halt the spread of SARS-CoV-2 and only very few treatment options are available to manage COVID-19 patients. For development of preclinical countermeasures, reliable and well-characterized small animal disease models will be of paramount importance. Here we show that intranasal inoculation of SARS-CoV-2 into Syrian...

sj-xlsx-2-tam-10.1177_1758835920977117 – Supplemental material for Low intratumor heterogeneity correlates with increased response to PD-1 blockade in renal cell carcinoma

Xia Ran, Jinyuan Xiao, Yi Zhang, Huajing Teng, Fang Cheng, Huiqian Chen, Kaifan Zhang & Zhongsheng Sun
Supplemental material, sj-xlsx-2-tam-10.1177_1758835920977117 for Low intratumor heterogeneity correlates with increased response to PD-1 blockade in renal cell carcinoma by Xia Ran, Jinyuan Xiao, Yi Zhang, Huajing Teng, Fang Cheng, Huiqian Chen, Kaifan Zhang and Zhongsheng Sun in Therapeutic Advances in Medical Oncology

Analysis of Collagen type X alpha 1 (COL10A1) expression and prognostic significance in gastric cancer based on bioinformatics

Shuai Chen, Yi Wei, Hanyang Liu, Yu Gong, Yan Zhou, Haojun Yang & Liming Tang
Collagen type X alpha 1 (COL10A1) is a member of the collagen family and the main matrix component. However, COL10A1 expression and prognosis relationship remains unclear in gastric cancer (GC). Through the analysis of database of Oncomine, the Cancer Genome Atlas (TCGA) as well as the Gene Expression Omnibus (GEO), in contrast to the tissue of normal gastric, COL10A1 in gastric cancer, had been upregulated. The high expression of COL10A1 was obviously related to T...

Identifying optimal capsid duplication length for the stability of reporter flaviviruses

Coleman Baker, Yang Liu, Jing Zou, Antonio Muruato, Xuping Xie & Pei-Yong Shi
Mosquito-transmitted flaviviruses cause widespread disease across the world. To provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable NanoLuc-tagged flaviviruses, including dengue virus serotypes 1-4, Japanese encephalitis virus, yellow fever virus, West Nile virus, and Zika virus. Since the reporter gene is often engineered at the capsid gene region, the capsid sequence must be duplicated to flank the reporter gene; such capsid duplication is essential for...

Registration Year

  • 2020
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  • Dataset
    82

Affiliations

  • Shanghai Jiao Tong University
    82
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    53
  • Capital Medical University
    46
  • Fudan University
    43
  • Sichuan University
    42
  • Sun Yat-sen University
    41
  • Zhejiang University
    41
  • Chinese Academy of Sciences
    40
  • Peking University
    38
  • Tongji University
    38