4 Works

NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Stephan Riesenberg, Nelly Helmbrecht, Philipp Kanis, Tomislav Maricic & Svante Pääbo
We engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications. The ‘Genome-editing Optimized Locked Design’ (GOLD)-gRNA increases genome editing efficiency up to around 1000-fold (from 0.08% to 80.5%) with a mean increase across different other targets of 7.4-fold. The related NGS data is deposited here.

Quantifying and reducing cross-contamination in single- and multiplex hybridization capture of ancient DNA

Elena Zavala, Ayinuer Aximu-Petri, Julia Richter, Birgit Nickel, Benjamin Vernot & Matthias Meyer
The use of hybridization capture has enabled a massive upscaling in sample sizes for ancient DNA studies, allowing the analysis of hundreds of skeletal remains (Mathieson et al., 2015; Narasimhan et al., 2019) or sediments (Vernot et al., 2021; Wang et al., 2021; Zavala et al., 2021) in single studies. Yet demands in throughput continue to grow, and hybridization capture has become a limiting step in sample preparation due to the large consumption of reagents,...

Coevolution of relative brain size and life expectancy in parrots

Simeon Q. Smeele, Dalia A. Conde, Annette Baudisch, Simon Bruslund, Andrew Iwaniuk, Johanna Staerk, Timothy F. Wright, Anna M. Young, Mary Brooke McElreath & Lucy Aplin
Previous studies have demonstrated a correlation between longevity and brain size in a variety of taxa. Little research has been devoted to understanding this link in parrots; yet parrots are well-known for both their exceptionally long lives and cognitive complexity. We employed a large-scale comparative analysis that investigated the influence of brain size and life history variables on longevity in parrots. Specifically, we addressed two hypotheses for evolutionary drivers of longevity: the Cognitive Buffer Hypothesis,...

How community forest management performs when REDD+ payments fail

Amy Collins, Mark Grote, Tim Caro, Aniruddha Ghosh, James Thorne, Jonathan Salerno & Monique Borgerhoff Mulder
The reduced emissions in deforestation and degradation (REDD+) initiative uses payments for ecosystem services as incentives for developing countries to manage and protect their forests. REDD+ initiatives also prioritize social (and environmental) co-benefits aimed at improving the livelihoods of communities that are dependent on forests. Despite the incorporation of co-benefits into REDD+ goals, carbon sequestration remains the primary metric for which countries can receive payments from REDD+, but after more than 10 years of REDD+,...

Registration Year

  • 2022

Resource Types

  • Dataset


  • Max Planck Institute for Evolutionary Anthropology
  • New Mexico State University
  • Max Planck Institute of Animal Behavior
  • Otterbein University
  • Colorado State University
  • University of Southern Denmark
  • University of Lethbridge
  • University of Bristol
  • University of California, Davis