In addition to the hallmark muscle stiffness, patients with recessive myotonia congenita (Becker disease) experience debilitating bouts of transient weakness that remain poorly understood despite years of study. We made intracellular recordings from muscle of both genetic and pharmacologic mouse models of Becker disease to identify the mechanism underlying transient weakness. Our recordings reveal transient depolarizations (plateau potentials) of the membrane potential to -25 to -35 mV in the genetic and pharmacologic models of Becker...
As of December 2020, COVID-19 has spread all over the world with more than 81 million cases and more than 1.8 million deaths. The rapidly increasing number of patients mandates the consideration of potential treatments for patients under severe and critical conditions. Convalescent plasma (CP) treatment refers to the approach of infusing patients with plasma from recently recovered patients. CP appears to be a possible therapeutic option to manage patients suffering from severe or even...
Multiple mutualism effects generate synergistic selection and strengthen fitness alignment in the interaction between legumes, rhizobia, and mycorrhizal fungiMichelle Afkhami, Maren Friesen & John Stinchcombe
Nearly all organisms participate in multiple mutualisms, and complementarity within these complex interactions can result in synergistic fitness effects. However, it remains largely untested how multiple mutualisms impact eco-evolutionary dynamics in interacting species. We tested how multiple microbial mutualists-- N-fixing bacteria and mycorrrhizal fungi-- affected selection and heritability of traits in their shared host plant (Medicago truncatula), as well as fitness alignment between partners. Our results demonstrate for the first time that multiple mutualisms synergistically...
Six- and seven-membered cucurbiturils form inclusion complexes with a lutidine derivative. These inclusion complexes differ in the binding mode, stability, kinetics of complexation, and sensitivity toward basic media.
Terminal Carboxylate Effects on the Thermodynamics and Kinetics of Cucurbituril Binding to Guests Containing a Central Bis(Pyridinium)-Xylylene SiteIago Neira, Marcos D. Garcia, Carlos Peinador & Angel Kaifer
A series of bis(pyridinium)-xylylene derivatives bearing carboxylate terminal groups were investigated as guests for the cucurbituril host in aqueous solution. While the presence of the terminal carboxylates has a modest effect on the thermodynamic stability of the complexes, the kinetics of complex association/dissociation is strongly affected. The relative position (meta, para) of the carboxylate group in relation to the pyridinium nitrogen also exerts a considerable effect on the binding kinetics.
The data collected represents photos, Geographic Information Systems (GIS) data, measured effects of our community engaged process, and associated metadata and process descriptions developed during our study of the Homestead and Little River communities in Miami Dade County.
In 2019, the Medical Library Association (MLA) adopted a new model of community governance and participation, referred to as the MLA Communities Transition. The Communities Transition was the culmination of long-ranging plans by MLA to support two of its strategic goals: Diversity and Inclusion, and Communities. The reorganization aimed to strengthen MLA member communities, better support programming, reduce administrative overhead, and attract new members. The 2019 - 2020 MLA Rising Stars cohort was tasked to...
Oceanic Advection Controls Mesoscale Mixed Layer Heat Budget and Air-sea Heat Exchange in the Southern OceanYu Gao, Igor Kamenkovich, Natalie Perlin & Ben Kirtman
We analyze the role of mesoscale heat advection in the Southern Ocean mixed layer (ML) heat budget using a regional high-resolution coupled model, with realistic atmospheric forcing and an idealized ocean component. In order to test if the overall current speed affect our conclusion, the simulations are carried out in two distinct cases: one with overall strong current (SC case) and the other with overall weak current (WC case). Mesoscale currents are defined with two...
1. Globally endangered ecosystems, like ephemeral wetlands, are often critical habitat for multiple interacting imperiled species. To conserve this biodiversity, managers must consider both species-specific resource requirements and mechanisms for endangered species coexistence under variable habitat conditions. 2. We examined communities native to California playa pools, ephemeral wetlands that have declined by >90% from their historic extent. Specifically, we describe the diet of a federally Threatened amphibian (Ambystoma californiense), and characterize interactions between this amphibian...
The contributions of individual traits to survival among terrestrial juvenile pond-breeding salamandersArianne Messerman & Manuel Leal
Individual survival is influenced by interactions between local environmental conditions and an organism’s morphological, behavioral, and physiological traits. Studies examining the effects of individual phenotypes on survival under variable conditions are relatively rare among early transitional life stages, though the vital rates of these life stages can importantly influence population dynamics. We experimentally examined the effects of initial body mass, movement, standard metabolic rate (SMR), and respiratory surface area water loss (RSAWL) on survival in...
Variation in leaf temperatures of tropical and subtropical trees are related to leaf thermoregulatory traits and not geographic distributions datasetOlga Tserej & Kenneth Feeley
To predict the effects of global warming on plants, previous studies have investigated how the distributions and physiological performances of plants relate to environmental temperatures. This approach implicitly assumes that leaf temperatures are tightly linked with regional air temperatures. However, the thermoregulatory behaviors and physical properties of leaves can differ greatly between species, leading to different plants having different leaf temperatures even when occurring under similar conditions. It is important to understand this variation in...
The formation of highly stable inclusion complexes in aqueous solution between the organometallic cobaltocenium cation (Cob+) and the hosts cucurbituril (CB7) and cucurbituril (CB8) was used to develop a simple method, based on UV–vis titrations, to assay the purity of samples of these two hosts. The equilibrium association constant (K) of the Cob+@CB7 complex had been previously reported by our group as 5.7 × 109 M–1 at 25 °C in 50 mM sodium acetate medium....
Thermodynamic Studies on the Cyclodextrin Complexation of Aromatic and Aliphatic Guests in Water and Water−Urea Mixtures. Experimental Evidence for the Interaction of Urea with Arene SurfacesLuis A. Godínez, Lauren Schwartz, Cecil M. Criss & Angel E. Kaifer
The thermodynamic functions that describe the complexation of a series of four carboxylic acid guests and two related quaternary ammonium derivatives with β-cyclodextrin were measured using flow calorimetry at 298 K in aqueous media in the presence and in the absence of large concentrations of urea. The relative differences of the thermodynamic parameters of complexation revealed the existence of specific interactions between urea and the aromatic surfaces of some of the nonpolar guests. As expected,...
Data for Interannual variability in the source location of North African dust transported to the AmazonCassandra Gaston, A Pourmand, J Longman, A Sharifi, Joseph Prospero, K Panechou, N Bakker, N Drake, D Guioiseau & Anne Barkley
Here, we present the data and MixSIAR code that corresponds to the manuscript “Interannual variability in the source location of North African dust transported to the Amazon.” African dust is seasonally transported to the western Tropical Atlantic Ocean (TAO) and South America (SA), including the Amazon Basin. Leading hypotheses suggest that either the Western North African potential source area (PSA) or the Central North African PSA (e.g., Bodélé Depression) is the main source of dust...
Introgression of alleles has emerged as an important avenue for genetic adaptation in both plant and animal populations. In vertebrates, adaptation to hypoxic high-altitude environments involves the coordination of multiple molecular and cellular mechanisms, including selection on the Hypoxia-Inducible Factor (HIF) pathway and the blood-O2 transport protein hemoglobin (Hb). In two Andean duck species, a striking DNA sequence similarity reflecting identity by descent is present across the ~20 kb b-globin cluster including both embryonic (HBE)...
Base-substitution mutation rate across the nuclear genome of Alpheus snapping shrimp and the timing of isolation by the Isthmus of PanamaKatherine Silliman, Jane Indorf, Nancy Knowlton, William Browne & Carla Hurt
The formation of the Isthmus of Panama and final closure of the Central American Seaway (CAS) provides an independent calibration point for examining the rate of DNA substitutions. This vicariant event has been widely used to estimate the substitution rate across mitochondrial genomes and to date evolutionary events in other taxonomic groups. Nuclear sequence data is increasingly being used to complement mitochondrial datasets for phylogenetic and evolutionary investigations; these studies would benefit from information regarding...
The multidimensional (and contrasting) effects of environmental warming on a group of montane tropical lizards: Lizard and plant dataMartha M Munoz, Kenneth Feeley, Patrick Martin & Vincent Farallo
1. Mountains are cradles for biodiversity and crucibles for climate-driven species loss, particularly for tropical ectotherms. Constriction on activity and amplified heat stress are two key sources of warming-driven vulnerability in tropical montane ectotherms. These threats, however, might be counterbalanced if rising temperatures also release organisms from limits on activity induced by cold stress. 2. We used biophysical modeling to estimate activity patterns and thermal stress under warming in a group of summit-dwelling Anolis lizards...
The cucurbituril (CB7) host forms rotaxane-type complexes with dumbbell-shaped, cationic guests bis(3,5-dimethoxybenzyl)-4,4′-bipyridinium (12+) and bis(3,5-diethoxybenzyl)-4,4′-bipyridinium (22+). The kinetics of complex formation is slower with the latter guest because of its bulkier end groups. Rotaxane formation was found to be thermodynamically more favorable and kinetically faster in D2O than in DMSO-d6 solution, which highlights the importance of hydrophobic interactions in the assembly of cucurbituril complexes.
Cyclobis(paraquat-p-phenylene) as a synthetic receptor for electron-rich aromatic compounds: electrochemical and spectroscopic studies of neurotransmitter bindingAngel E. Kaifer, J. Fraser Stoddart & Anna R. Bernardo
The dicationic guest 2,7-dimethyldiazaphenanthrenium forms a fluorescent inclusion complex with the cucurbituril host, which can be used to effectively bind and detect indole derivatives, such as serotonin and tryptophan.
(2018). Binding of bambusuril with bipyridinium guests in water. Supramolecular Chemistry: Vol. 30, SI: ISMSC, pp. 832-837.
Data from: Positive allosteric modulation of the α7 nicotinic acetylcholine receptor as a treatment for cognitive deficits after traumatic brain injuryDavid J. Titus, Timothy Johnstone, Nathan H. Johnson, Sidney H. London, Meghana Chapalamadugu, Derk Hogenkamp, Kelvin W. Gee & Coleen M. Atkins
Cognitive impairments are a common consequence of traumatic brain injury (TBI). The hippocampus is a subcortical structure that plays a key role in the formation of declarative memories and is highly vulnerable to TBI. The α7 nicotinic acetylcholine receptor (nAChR) is highly expressed in the hippocampus and reduced expression and function of this receptor are linked with cognitive impairments in Alzheimer’s disease and schizophrenia. Positive allosteric modulation of α7 nAChRs with AVL-3288 enhances receptor currents...
Comparison of adult census size and effective population size support the need for continued protection of two Solomon Island endemicsSarah A. Cowles, Brian C. Weeks, Lindsey Perrin, Nancy Chen & J. Albert C. Uy
Because a population’s ability to respond to rapid change is dictated by standing genetic variation, we can better predict a population’s long-term viability by estimating and then comparing adult census size (N) and effective population size (Ne). However, most studies only measure N or Ne, which can be misleading. Using a combination of field and genomic sequence data, we here estimate and compare N and Ne in two range-restricted endemics of the Solomon Islands. Two...
Area and Timing data and R script for: 3D scanning as a tool to measure growth rates of live coral microfragments used for coral reef restorationHanna Koch, Bailey Wallace, Allyson DeMerlis, Abigail Clark & Robert Nowicki
Rapid and widespread declines in coral health and abundance have driven increased investments in coral reef restoration interventions to jumpstart population recovery. Microfragmentation, an asexual propagation technique, is used to produce large numbers of corals for research and restoration. As part of resilience-based restoration, coral microfragments of different genotypes and species are exposed to various stressors to identify candidates for propagation. Growth rate is one of several important fitness-related traits commonly used in candidate selection,...
University of Miami42
Department of Physical and Technical Sciences2
Columbus State University1
Wright-Patterson Air Force Base1
Consortium for Sustainable Development of the Andean Ecoregion1
University of Michigan-Ann Arbor1
University of Michigan–Ann Arbor1
Natural History Museum of Los Angeles County1