2 Works

Data from: High-resolution chemical dissection of a model eukaryote reveals targets, pathways and gene functions

Dominic Hoepfner, Stephen B. Helliwell, Heather Sadlish, Sven Schuierer, Ireos Filipuzzi, Sophie Brachat, Bhupinder Bhullar, Uwe Plikat, Yann Abraham, Marc Altorfer, Thomas Aust, Lukas Baeriswyl, Raffaele Cerino, Lena Chang, David Estoppey, Juerg Eichenberger, Mathias Frederiksen, Nicole Hartmann, Annika Hohendahl, Britta Knapp, Philipp Krastel, Nicolas Melin, Florian Nigsch, Edward J. Oakeley, Virginie Petitjean … & N. Rao Movva
Due to evolutionary conservation of biology, experimental knowledge captured from genetic studies in eukaryotic model organisms provides insight into human cellular pathways and ultimately physiology. Yeast chemogenomic profiling is a powerful approach for annotating cellular responses to small molecules. Using an optimized platform, we provide the relative sensitivities of the heterozygous and homozygous deletion collections for nearly 1800 biologically active compounds. The data quality enables unique insights into pathways that are sensitive and resistant to...

Data from: Identification and evaluation of novel acetolactate synthase inhibitors as antifungal agents

Daryl L. Richie, Katherine V. Thompson, Vivian Prindle, Thomas Aust, Ralph Riedl, Jianshi Tao, Jessica A. Sexton, Thomas Zabawa, Joseph Drumm, Simona Cotesta, Jürg Eichenberger, Sven Schuierer, Nicole Hartmann, N. Rao Movva, John A. Tallarico, Neil S. Ryder & Dominic Hoepfner
High-throughput phenotypic screening against yeast Saccharomyces cerevisiae revealed a series of triazolo-pyrimidine-sulfonamide compounds with broad-spectrum antifungal activity, no significant cytotoxicity, and low protein binding. To elucidate the target of this series we have applied a chemogenomic profiling approach using the S. cerevisiae deletion collection. All compounds of the series yielded highly similar profiles that suggested acetolactate synthase (Ilv2p, catalyzes the first common step in branched chain amino acid biosynthesis) as a possible target. High correlation...

Registration Year

  • 2013

Resource Types

  • Dataset


  • Novartis (Switzerland)