4 Works

Data from: A new specimen of Agorophius pygmaeus (Agorophiidae, Odontoceti, Cetacea) from the Early Oligocene Ashley Formation of South Carolina, USA

Stephen J. Godfrey, Mark D. Uhen, Jason E. Osborne & Lucy E. Edwards
The holotype partial skull of Agorophius pygmaeus (the monotypic form for both the genus Agorophius and the Family Agorophiidae) has been missing for approximately 140 years. Since the discovery of Agorophius pygmaeus, many additional taxa and specimens have been placed in the Family Agorophiidae, only to be reclassified and removed later. This has created confusion as to what is and what is not an agorophiid and a lack of clarity as to what characteristics delimit...

Data from: Systems analysis of adaptive responses to MAP Kinase pathway blockade in BRAF mutant melanoma

Brian J. Capaldo, Devin Roller, Mark J. Axelrod, Alex F. Koeppel, Emanuel F. Petricoin, Craig L. Slingluff, Michael J. Weber, Aaron J. Mackey, Daniel Gioeli & Stefan Bekiranov
Fifty percent of cutaneous melanomas are driven by activated BRAFV600E, but tumors treated with RAF inhibitors, even when they respond dramatically, rapidly adapt and develop resistance. Thus, there is a pressing need to identify the major mechanisms of intrinsic and adaptive resistance and develop drug combinations that target these resistance mechanisms. In a combinatorial drug screen on a panel of 12 treatment-naïve BRAFV600E mutant melanoma cell lines of varying levels of resistance to mitogen-activated protein...

Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar

Morgan R. Gostel, Kiera A. Coy & Andrea Weeks
Developing effective and cost-efficient multilocus nuclear datasets for angiosperm species is a continuing challenge to the systematics community. Here we describe the development and validation of a novel set of 91 nuclear markers for PCR-based target enrichment. Using microfluidic PCR and Illumina MiSeq, we generated nuclear, subgenomic libraries for 96 species simultaneously and sequenced them for a total cost of ca. $6000 USD. Approximately half of these costs include reusable reagents (primers, barcodes, and custom...

Data from: In-solution hybridization for mammalian mitogenome enrichment: pros, cons and challenges associated with multiplexing degraded DNA

Melissa T. R. Hawkins, Courtney A. Hofman, Taylor Callicrate, Molly M. McDonough, Mirian T. N. Tsuchiya, Eliécer E. Gutierrez, Kristofer M. Helgen & Jesús E. Maldonado
Here, we present a set of RNA-based probes for whole mitochondrial genome in-solution enrichment, targeting a diversity of mammalian mitogenomes. This probes set was designed from seven mammalian orders and tested to determine the utility for enriching degraded DNA. We generated 63 mitogenomes representing five orders and 22 genera of mammals that yielded varying coverage ranging from 0 to >5400X. Based on a threshold of 70% mitogenome recovery and at least 10× average coverage, 32...

Registration Year

  • 2015
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Resource Types

  • Dataset
    4

Affiliations

  • George Mason University
    4
  • Smithsonian Conservation Biology Institute
    2
  • University of Virginia
    1
  • Smithsonian Institution
    1
  • University of Maryland, College Park
    1