14 Works

Data from: Phylogenomic analysis of transcriptome data elucidates co-occurrence of a paleopolyploid event and the origin of bimodal karyotypes in Agavoideae (Asparagaceae)

Michael R. McKain, Norman Wickett, Yeting Zhang, Saravanaraj Ayyampalayam, W. Richard McCombie, Mark W. Chase, J. Chris Pires, Claude W. DePamphilis, Jim Leebens-Mack & Claude W. De Pamphilis
PREMISE OF THE STUDY: The stability of the bimodal karyotype found in Agave and closely related species has long interested botanists. The origin of the bimodal karyotype has been attributed to allopolyploidy, but this hypothesis has not been tested. Next Generation transcriptome sequence data were used to test whether a paleopolyploid event occurred on the same branch of the Agavoideae phylogenetic tree as the origin of the Yucca-Agave bimodal karyotype. METHODS: Illumina RNAseq data were...

Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq

Xiaoyin Chen, Yu-Chi Sun, George M. Church, Je Hyuk Lee & Anthony M. Zador
Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices spatial resolution, whereas manual cell picking has low throughput. In situ sequencing could potentially achieve both high spatial resolution and high throughput, but current in situ sequencing techniques are inefficient at reading out cellular barcodes. Here we describe...

Data from: Comparative phylogeographic inference with genome-wide data from aggregated population-pairs

Alexander T. Xue & Mike Hickerson
Comparing divergences across multiple sister population-pairs has been a focus in phylogeography since its inception. Initial approaches utilized organelle genetic data and involved qualitative comparisons of phylogenetic patterns to evaluate hypotheses of shared and variable evolutionary responses. This endeavor has progressed with coalescent model-based statistical techniques and advances in next-generation sequencing, yet there remains a need for methods that can exploit aggregated genomic-scale data within a unified analytical framework. To this end, we introduce the...

The chicken pan-genome reveals gene content variation and a promoter region deletion in IGF2BP1 affecting body size

Kejun Wang, Haifei Hu, Yadong Tian, Jingyi Li, Armin Scheben, Chenxi Zhang, Yiyi Li, Junfeng Wu, Lan Yang, Xuewei Fan, Guirong Sun, Donghua Li, Yanhua Zhang, Ruili Han, Ruirui Jiang, Hetian Huang, Fengbin Yan, Yanbin Wang, Zhuanjian Li, Guoxi Li, Xiaojun Liu, Wenting Li, David Edwards & Xiangtao Kang
Domestication and breeding have reshaped the genomic architecture of chicken, but the retention and loss of genomic elements during these evolutionary processes remain unclear. We present the first chicken pan-genome constructed using 664 individuals, which identified an additional ∼66.5 Mb sequences that are absent from the reference genome (GRCg6a). The constructed pan-genome encoded 20,491 predicated protein-coding genes, of which higher expression level are observed in conserved genes relative to dispensable genes. Presence/absence variation (PAV) analyses...

Co-expression of calcium and hERG potassium channels reduces the incidence of proarrhythmic events

Jamie Vandenberg, Sara Ballouz, Melissa M Mangala, Matthew D Perry, Stewart Heitmann, Jesse A Gillis, Adam P Hill & Jamie I Vandenberg
Abstract Aims Cardiac electrical activity is extraordinarily robust. However, when it goes wrong it can have fatal consequences. Electrical activity in the heart is controlled by the carefully orchestrated activity of more than a dozen different ion conductances. Whilst there is considerable variability in cardiac ion channel expression levels between individuals, studies in rodents have indicated that there are modules of ion channels whose expression co-vary. The aim of this study was to investigate whether...

Data from: Genome-wide patterns of transposon proliferation in an evolutionary young hybrid fish

Stefan Dennenmoser, Fritz J. Sedlazeck, Michael C. Schatz, Janine Altmüller, Matthias Zytnicki & Arne W. Nolte
Hybridization can induce transposons to jump into new genomic positions, which may result in their accumulation across the genome. Alternatively, transposon copy numbers may increase through non-allelic (ectopic) homologous recombination in highly repetitive regions of the genome. The relative contribution of transposition bursts versus recombination-based mechanisms to evolutionary processes remains unclear because studies on transposon dynamics in natural systems are rare. We assessed the genome-wide distribution of transposon insertions in a young hybrid lineage (“invasive...

Data from: Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer

Mamoru Kato, Daniel A. Vasco, Ryuichi Sugino, Daichi Narushima & Alexander Krasnitz
Single-cell sequencing is a promising technology that can address cancer cell evolution by identifying genetic alterations in individual cells. In a recent study, genome-wide DNA copy numbers of single cells were accurately quantified by single-cell sequencing in breast cancers. Phylogenetic tree analysis revealed genetically distinct populations, each consisting of homogeneous cells. Bioinformatics methods based on population genetics should be further developed to quantitatively analyse the single-cell sequencing data. We developed a bioinformatics framework that was...

Data from: Long-term evolution on complex fitness landscapes when mutation is weak

David M. McCandlish
Understanding evolution on complex fitness landscapes is difficult both because of the large dimensionality of sequence space and the stochasticity inherent to population-genetic processes. Here I present an integrated suite of mathematical tools for understanding evolution on time-invariant fitness landscapes when mutations occur sufficiently rarely that the population is typically monomorphic and evolution can be modeled as a sequence of well-separated fixation events. The basic intuition behind this suite of tools is that surrounding any...

Data from: Looking at cerebellar malformations through text-mined interactomes of mice and humans

Ivan Iossifov, Raul Rodriguez-Esteban, Ilya Mayzus, Kathleen J. Millen & Andrey Rzhetsky
We have generated and made publicly available two very large networks of molecular interactions: 49,493 mouse-specific and 52,518 human-specific interactions. These networks were generated through automated analysis of 368,331 full-text research articles and 8,039,972 article abstracts from the PubMed database, using the GeneWays system. Our networks cover a wide spectrum of molecular interactions, such as bind, phosphorylate, glycosylate, and activate; 207 of these interaction types occur more than 1,000 times in our unfiltered, multi-species data...

Data from: Genome-wide discovery and characterization of maize long non-coding RNAs

Lin Li, Steven R. Eichten, Rena Shimizu, Katherine Petsch, Cheng-Ting Yeh, Wei Wu, Michael J. Scanlon, Jianming Yu, Patrick S. Schnable, Marja C. P. Timmermans, Nathan M. Springer & Gary J. Muehlbauer
Background: Long noncoding RNAs (lncRNAs) are transcripts that are 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics and expression inheritance pattern of lncRNAs in maize are still largely unknown. Results: By exploiting available public ESTs, maize whole genome sequence annotation and RNA-seq datasets from 30 different experiments, we identified 20,163 putative lncRNA. Of these lncRNAs, more than 90% are predicted to be...

Data from: Tensor analysis reveals distinct population structure that parallels the different computational roles of areas M1 and V1

Jeffrey S. Seely, Matthew T. Kaufman, Stephen I. Ryu, Krishna V. Shenoy, John P. Cunningham & Mark M. Churchland
Cortical firing rates frequently display elaborate and heterogeneous temporal structure. One often wishes to compute quantitative summaries of such structure—a basic example is the frequency spectrum—and compare with model-based predictions. The advent of large-scale population recordings affords the opportunity to do so in new ways, with the hope of distinguishing between potential explanations for why responses vary with time. We introduce a method that assesses a basic but previously unexplored form of population-level structure: when...

Variable and sexually conflicting selection on Silene stellata floral traits by a putative moth pollinator selective agent

Juannan Zhou, Richard J. Reynolds, Elizabeth A. Zimmer, Michele R. Dudash & Charles B. Fenster
Conflicting selection is an important evolutionary mechanism since it impedes directional evolution and helps to maintain phenotypic variation. It can arise when mutualistic and antagonistic selective agents exert opposing selection on the same trait and when distinct phenotypic optima are favored by different fitness components. In this study, we test for conflicting selection through different sexual functions of the hermaphroditic plant, Silene stellata during its early and late flowering season. We find selection is consistently...

The human origin recognition complex (ORC) is essential for pre-RC assembly, mitosis and maintenance of nuclear structure

Hsiang-Chen Chou, Kuhulika Bhalla, Osama-El Demerdesh, Olaf Klingbeil, Kaarina Hanington, Sergey Aganezov, Peter Andrews, Habeeb Alsudani, Kenneth Chang, Christopher R. Vakoc, Michael C. Schatz, Richard W. McCombie & Bruce Stillman
The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an auxin-inducible degradation system, we created stable cell lines capable of ablating ORC2 rapidly, revealing multiple cell division cycle phenotypes. The primary defects in the absence of ORC2 were cells encountering difficulty in initiating DNA...

Data from: Genotype-environment mismatch of kelp forests under climate change

Sofie Vranken, Thomas Wernberg, Armin Scheben, Anita Severn-Ellis, Jacqueline Batley, Philipp Emanuel Bayer, David Edwards, David Wheeler & Melinda Ann Coleman
Climate change is increasingly impacting ecosystems globally. Understanding adaptive genetic diversity and whether it will keep pace with projected climatic change is necessary to assess species’ vulnerability and design efficient mitigation strategies such as assisted adaptation. Kelp forests are the foundations of temperate reefs globally but are declining in many regions due to climate stress. A lack of knowledge of kelps’ adaptive genetic diversity hinders assessment of vulnerability under extant and future climates. Using 4245...

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  • Cold Spring Harbor Laboratory
  • Johns Hopkins University
  • University of Western Australia
  • Central Sericultural Germplasm Resources Centre
  • National Cancer Center
  • Howard Hughes Medical Institute
  • Stanford University
  • Columbia University
  • University of Cologne
  • Department of Plant Biology