2 Works

Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq

Xiaoyin Chen, Yu-Chi Sun, George M. Church, Je Hyuk Lee & Anthony M. Zador
Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices spatial resolution, whereas manual cell picking has low throughput. In situ sequencing could potentially achieve both high spatial resolution and high throughput, but current in situ sequencing techniques are inefficient at reading out cellular barcodes. Here we describe...

Data from: Sweepstake evolution revealed by population-genetic analysis of copy-number alterations in single genomes of breast cancer

Mamoru Kato, Daniel A. Vasco, Ryuichi Sugino, Daichi Narushima & Alexander Krasnitz
Single-cell sequencing is a promising technology that can address cancer cell evolution by identifying genetic alterations in individual cells. In a recent study, genome-wide DNA copy numbers of single cells were accurately quantified by single-cell sequencing in breast cancers. Phylogenetic tree analysis revealed genetically distinct populations, each consisting of homogeneous cells. Bioinformatics methods based on population genetics should be further developed to quantitatively analyse the single-cell sequencing data. We developed a bioinformatics framework that was...

Registration Year

  • 2017

Resource Types

  • Dataset


  • Cold Spring Harbor Laboratory
  • National Cancer Center
  • Harvard Medical School