Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in...

Additional file 1: Table S1. Summary information on the ATAC-seq samples investigated in this study. Table S2. Summary information on the RNA-seq samples investigated in this study. Table S3. The information of download data. Table S4. Comparison with peaks of previous study designed by zhou et al. Table S5. Motif enrichment analysis of shared peaks. Table S6. The information of PERV overlapped peaks. Table S7. The information of GWAS SNPs which located in peak regions....

Additional file 2: Fig. S1. The analysis process and characteristics of peaks. (a) and (b) The ATAC-seq signal enrichment around TSSs and insert size distribution of ATAC-seq for 2 samples (brain_sample1 and brain_sample2). (c) Proportion of tissue-specific peaks that were distal peaks or proximal peaks. (d) Distribution of peak size obtained in this study. Fig. S2. Identifying the functional pathway and tissue specificity via RNA-seq. (a) Hierarchical clustering using gene expression. (b) Functional enrichment of...

With no clear end for the outbreak, identifying the drugs that are effective in COVID-19’s management is of utmost importance to reduce the impact on the general population and the healthcare systems. This is a systematic review and a meta-analysis evaluating the evidence from clinical trials on the effect of colchicine and corticosteroids against COVID-19. In this review, we have systematically searched five databases [(PubMed, Embase, clinicaltrials.gov, ICTRP, CINAHL (EBSCO)]. Cochrane’s data extraction sheet was...

With no clear end for the outbreak, identifying the drugs that are effective in COVID-19’s management is of utmost importance to reduce the impact on the general population and the healthcare systems. This is a systematic review and a meta-analysis evaluating the evidence from clinical trials on the effect of colchicine and corticosteroids against COVID-19. In this review, we have systematically searched five databases [(PubMed, Embase, clinicaltrials.gov, ICTRP, CINAHL (EBSCO)]. Cochrane’s data extraction sheet was...

Climatic variation is a key driver of genetic differentiation and phenotypic traits evolution, and local adaptation to temperature is expected in widespread species. We investigated phenotypic and genomic changes in the native range of the Asian tiger mosquito, Aedes albopictus. We first refine the phylogeographic structure based on genome-wide regions (1,901 double-digest restriction-site associated DNA single nucleotide polymophisms [ddRAD SNPs]) from 41 populations. We then explore the patterns of cold adaptation using phenotypic traits measured...

With the ongoing COVID-19 pandemic and the emergence of various SARS-CoV-2 variants, a comprehensive evaluation of long-term efficacy of antibody response in convalescent individuals is urgently needed. Several longitudinal studies had reported the antibody dynamics after SARS-CoV-2 acute infection, but the follow-up was mostly limited to 1 year or 18 months at the maximum. In this study, we investigated the durability, potency, and susceptibility to immune evasion of SARS-CoV-2-specific antibody in COVID-19 convalescents for 2...

Research on fern ecology has gained attention in the last decade, yet there is a paucity of information on the comparison of ferns communities across continents. This study focused on comparing the ferns community assemblages in some tropical forests of Malaysia and Nigeria, thereby assessing the patterns of species richness (SR) and phylogenetic diversity(PD) in relation to the bioclimatic drivers across the continents. The diversity and taxonomic compositions of ferns were assessed using 180 plots...

Additional file 3: Table S1. The ASVs in co-occurrence pattern of trial 1 stage 4.

Additional file 1: Table S1. Summary information on the ATAC-seq samples investigated in this study. Table S2. Summary information on the RNA-seq samples investigated in this study. Table S3. The information of download data. Table S4. Comparison with peaks of previous study designed by zhou et al. Table S5. Motif enrichment analysis of shared peaks. Table S6. The information of PERV overlapped peaks. Table S7. The information of GWAS SNPs which located in peak regions....

Additional file 2: Fig. S1. The analysis process and characteristics of peaks. (a) and (b) The ATAC-seq signal enrichment around TSSs and insert size distribution of ATAC-seq for 2 samples (brain_sample1 and brain_sample2). (c) Proportion of tissue-specific peaks that were distal peaks or proximal peaks. (d) Distribution of peak size obtained in this study. Fig. S2. Identifying the functional pathway and tissue specificity via RNA-seq. (a) Hierarchical clustering using gene expression. (b) Functional enrichment of...

Additional file 2: Table S4. Proteomic analysis of the intracellular and secretome in P. putida KT2440.

Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in...

The miscibility between hydrophobic and hydrophilic biopolymers has been of significant challenge. This study used a novel simplified chitin modification method to produce phthalic - chitin using phthalic anhydride in a substitution reaction. The FT-IR functional group analysis was used to confirm the substitution reaction. The modified chitin was used as compatibiliser in PLA/starch biocomposite to enhance its properties. The biocomposite was prepared using melt extrusion and compression moulding technique. The biocomposite's morphological, thermomechanical, and...

Additional file 3: Table S1. The ASVs in co-occurrence pattern of trial 1 stage 4.

With the ongoing COVID-19 pandemic and the emergence of various SARS-CoV-2 variants, a comprehensive evaluation of long-term efficacy of antibody response in convalescent individuals is urgently needed. Several longitudinal studies had reported the antibody dynamics after SARS-CoV-2 acute infection, but the follow-up was mostly limited to 1 year or 18 months at the maximum. In this study, we investigated the durability, potency, and susceptibility to immune evasion of SARS-CoV-2-specific antibody in COVID-19 convalescents for 2...

Additional file 2: Table S4. Proteomic analysis of the intracellular and secretome in P. putida KT2440.